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Design of a Non-destructive Fluorescent-image-based Fresh Meat Contamination Inspection System
( Yi-chich Chiu ),( Yi-ning Ho ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.1
Health and safety of meat can be public concerns.Moreover, sanitation monitoring of a processing plant or a processing line is directly related to food safety.This research aims to develop a non-destructive contamination inspection system for livestock fresh food products. Fluorescent image analyzing techniques is used to detect and identify food product contamination of Escherichia coli.Fluorescence spectra data of these food product contamination is established to provide early detection of food safety problems to reduce food safety incidents.The potential of fluorescence spectroscopy was investigated for the non-destructive evaluation of Escherichia coli content and plate count on pork meat surface stored aerobically at 14 °C during. Use of excitation light and emission wavelength 200nm~400nm Excitation (Ex) Emission (Em) Matrix of fluorescence intensity was obtained and fluorescence from Escherichia coli (Ex=295 nm and Em=330 nm) was detected. Microbial spoilage on meat could be detected from fluorescence. On pork meat surface, Escherichia coli content and plate count showed high correlation. Therefore of Escherichia coli content could be a parameter of sanitation monitoring.In the Excitation Emission Matrix of fluorescence intensity, the fluorescence of of Escherichia coli was detected. Main tasks of this study include developing systems and technologies to inspect and etermine whether pork were contaminated by E. coli. Fluorescence detection and analysis systems, is design and developed based on LabVIEW graphical programing language.
Zhe-Xue Quan,Dan-Ning Zeng,Yi-Ping Xiao,노성운,Young-Do Nam,Ho-Won Chang,윤정훈,오희목,배진우 한국미생물학회 2009 The journal of microbiology Vol.47 No.2
A bacterial strain, designated Iso4T, was isolated from the East Sea of Korea and was subjected to a polyphasictaxonomy study including phenotypic and chemotaxonomic characteristics as well as 16S rRNAgene sequence analysis. Cells of the strain were Gram-negative, motile, non-budding, non-stalked, andstrictly aerobic. Strain Iso4T grew optimally at 20°C in the presence of 1~2% (w/v) NaCl and at pH 6.9~7.6. The major respiratory quinone was Q-10 and the major cellular fatty acids were C18:1 ω7c (53.5%),C17:1 ω5c (11.7%), C17:1 ω6c (8.1%), C16:0 (7.8%), C17:0 (4.8%), C15:0 (2.9%), and C16:1 ω5c (2.2%). The DNAG+C content of strain Iso4T was 56.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequencesshowed that strain Iso4T formed a monophyletic clade in the family Hyphomonadaceae, supported by highbootstrap value and was most closely related to the genus Hyphomonas (92~94%), a member of marinebacteria in the family. The phenotypic, genotypic, and chemotaxonomic evidences also suggest strain Iso4Trepresents a novel genus and species in the family Hyphomonadaceae, for which the name Henriciella gen. nov., sp. nov. is proposed. The type strain is Iso4T (=KCTC 12513T =DSM 19595T =JCM 15116T).