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- 저자 : ( Xinglin Zhang ) (검색결과 5 건)
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Identification and Characterization of EDT1 Conferring Drought Tolerance in Rice
Tao Wu,Mingxing Zhang,Hongjia Zhang,Kai Huang,Mojun Chen,Chen Chen,Xue Yang,Zhao Li,Haoyuan Chen,Zhiming Ma,Xunming Zhang,Wenzhu Jiang,Xinglin Du 한국식물학회 2019 Journal of Plant Biology Vol.62 No.1
Basic Leucine Zipper (bZIP) transcription factors(TFs) play important roles in many processes, especially inabiotic stress response in plants. In this study, we characterizeda new gene EHANCED DROUGHT TOLERANCE 1 (EDT1),member of group E of bZIP transcription factor family inrice. The EDT1 protein contains one bZIP domain, oneputative nuclear localization signal (NLS) and six conservedphosphorylation sites. The expression of EDT1 is suppressedby several abiotic stresses, such as cold, droutht, and salt. Corresponding with expression patterns, several stress-associatedcis-acting elements were found in the EDT1 promoter. Theresults of subcellular localization and transactivation abilityanalyses indicated that EDT1 was localized in the nucleusand functioned as a nuclear protein, with its transactivationactivity primarily located in N-terminal. Transgenic riceoverexpressing EDT1 showed drought tolerance that hasbeen significantly improved. Real-time PCR analysis revealedthat some stress-related genes, such as OsbZIP12, SNAC1,OsLEA3, OsbZIP16, OsbZIP10 and OsABI2 were up-regulatedin EDT1 overexpression lines. These results indicate thatEDT1 plays a positive role in drought tolerance and providesvaluable targets for breeding drought-tolerant rice cultivars.
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Jiaqi Zhang,Quanhui Hou,Yanbing Liu,Xinglin Yang 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.118 No.-
The design of catalysts with excellent catalytic activity plays an important role in the field of solid-statehydrogen storage of new energy sources. Herein, a novel hydrangea-like NiO@NiMoO4 composite catalystwas prepared through a facile hydrothermal reaction. Subsequently, NiO@NiMoO4 was doped into MgH2by ball milling to solve the problems of high dehydrogenation temperature and slow desorption kineticsof MgH2. It can be seen from the experimental results that the MgH2 + 10 wt% NiO@NiMoO4 compositestarts to dehydrogenate at about 190 C, which is about 170 C lower than that of pure MgH2. Meanwhile,after complete dehydrogenation, the composites can start to absorb hydrogen below 40 C. Comparedwith pure MgH2, the activation energy of hydrogen absorption and dehydrogenation of the compositedecreased by 47.6 kJ/mol and 46.5 kJ/mol, respectively. In 10th cycle tests, the MgH2 + 10 wt%NiO@NiMoO4 composite still has good cycle stability. After adding a small amount of biomass charcoal,the hydrogen storage capacity can even be maintained above 97%. Furthermore, the characterizationresults show that the in situ generated new species Mo and Mg2Ni/Mg2NiH4 synergistically promotethe adsorption and dissociation of hydrogen. This new synergistic mechanism provides new comprehensiveinsights for improving reversible hydrogen storage in MgH2.
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Zhao Chijun,Jia Xinglin,Pan Yanying,Liao Simeng,Zhang Shuo,Ji Chunxiao,Kuang Guangwei,Wu Xin,Liu Quan,Tang Yulong,Fang Lihua 한국미생물학회 2023 The journal of microbiology Vol.61 No.4
Streptococcus suis serotype 2 (SS2) is an important zoonotic pathogen that can infect humans in contact with infected pigs or their byproducts. It can employ different types of genes to defend against oxidative stress and ensure its survival. The thioredoxin (Trx) system is a key antioxidant system that contributes adversity adaptation and pathogenicity. SS2 has been shown to encode putative thioredoxin genes, but the biological roles, coding sequence, and underlying mechanisms remains uncharacterized. Here, we demonstrated that SSU05_0237-ORF, from a clinical SS2 strain, ZJ081101, encodes a protein of 104 amino acids with a canonical CGPC active motif and an identity 70–85% similar to the thioredoxin A (TrxA) in other microorganisms. Recombinant TrxA efficiently catalyzed the thiol-disulfide oxidoreduction of insulin. The deletion of TrxA led to a significantly slow growth and markedly compromised tolerance of the pathogen to temperature stress, as well as impaired adhesion ability to pig intestinal epithelial cells (IPEC-J2). However, it was not involved in H2O2 and paraquat-induced oxidative stress. Compared with the wild-type strain, the ΔTrxA strain was more susceptible to killing by macrophages through increasing NO production. Treatment with TrxA mutant strain also significantly attenuated cytotoxic effects on RAW 264.7 cells by inhibiting inflammatory response and apoptosis. Knockdown of pentraxin 3 in RAW 264.7 cells was more vulnerable to phagocytic activity, and TrxA promoted SS2 survival in phagocytic cells depending on pentraxin 3 activity compared with the wild-type strain. Moreover, a co-inoculation experiment in mice revealed that TrxA mutant strain is far more easily cleared from the body than the wild type strain in the period from 8–24 h, and exhibits significantly attenuated oxidative stress and liver injury. In summary, we reveal the important role of TrxA in the pathogenesis of SS2.
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HS-Sign: A Security Enhanced UOV Signature Scheme Based on Hyper-Sphere
( Jiahui Chen ),( Shaohua Tang ),( Xinglin Zhang ) 한국인터넷정보학회 2017 KSII Transactions on Internet and Information Syst Vol.11 No.6
For “generic” multivariate public key cryptography (MPKC) systems, experts believe that the Unbalanced Oil-Vinegar (UOV) scheme is a feasible signature scheme with good efficiency and acceptable security. In this paper, we address two problems that are to find inversion solution of quadratic multivariate equations and find another structure with some random Oil-Oil terms for UOV, then propose a novel signature scheme based on hyper-sphere (HS-Sign for short) which directly answers these two problems. HS-Sign is characterized by its adding Oil-Oil terms and more advantages compared to UOV. On the one side, HS-Sign is based on a new inversion algorithm from hyper-sphere over finite field, and is shown to be a more secure UOV-like scheme. More precisely, according to the security analysis, HS-Sign achieves higher security level, so that it has larger security parameters choice ranges. On the other side, HS-Sign is beneficial from both the key side and computing complexity under the same security level compared to many baseline schemes. To further support our view, we have implemented 5 different attack experiments for the security analysis and we make comparison of our new scheme and the baseline schemes with simulation programs so as to show the efficiencies. The results show that HS-Sign has exponential attack complexity and HS-Sign is competitive with other signature schemes in terms of the length of the message, length of the signature, size of the public key, size of the secret key, signing time and verification time.
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Research on the Development of E-commerce for Poverty Alleviation in Wumeng Mountain Area of Yunnan
Hong Runzhang,Huang Congyan,Zhang Lanlan,Gao Xinglin 아시아사회과학학회 2020 Jornal of Asia Social Science Vol.1 No.3
Under the background of poverty alleviation, aiming at realizing the scientific, large-scale and intelligent development of Wumeng Mountain’s industry and the overall revitalization of the countryside, it is important to consider how to make good use of e-commerce tools in Wumeng Mountain, Yunnan, fully understand the importance of e-commerce poverty alleviation, combine the characteristics and advantages of regional industrial development, effectively resolve the constraints in the process of e-commerce poverty alleviation, optimize the combination of different e-commerce poverty alleviation models, innovate the development mechanism, and scientifically implement e-commerce poverty alleviation.
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