기획주제(企劃主題) : 동아시아 한자(漢字),한자교육(漢字敎育)의 현황(現況)과 과제(課題); ?圈識字法與天津市低年級識字?學

( Xin Ying Fu ),( Jing Yi Liu ) 한국한자한문교육학회 2013 漢字 漢文敎育 Vol.30 No.-

지연반복한자교육법(?圈識字)은 천진의 초등학교 저학년의 한자교육에서 매우 중요한 지위를 점하고 있으며, 그 효과 또한 실제적으로 증명된다. 천진에서의 이런 경험은 다른 한자교육법과 비교해 볼 때, 지연반복한자교육법은 아동의 기억현상과 부합되며, 그 효과 또한 매우 높게 나타난다. 저학년 한자교육은 지연반복한자교육법이 핵심이 되며, 다른 기타 한자교육법은 부가 되어, 이러한 교육 경험을 확대시킬 필요가 있다. 이는 집중한자교육의 이념을 구현한 것이나 그 스스로도 독특한 특징을 가지고 있다. “Delayed repetition literacy” stands an important position on literacy teaching in lower grade in Tianjin, and the effects is obvious. Relative to others, this literacy teaching accords with children`s memory rule, and promotes the teaching effects. It embody the ideal of the “focus literacy”, but also has its own characters and so, deserves popularizing.

VSV-G Viral Envelope Glycoprotein Prepared from Pichia pastoris Enhances Transfection of DNA into Animal Cells

( Xin Liu ),( Ying Dong ),( Jingquan Wang ),( Long Li ),( Zhenmin-zhong ),( Yun-pan Li ),( Shao-jun Chen ),( Yu-cai Fu ),( Wen-can Xu ),( Chi-ju Wei ) 한국미생물생명공학회(구 한국산업미생물학회) 2017 Journal of microbiology and biotechnology Vol.27 No.6

Vesicular stomatitis virus G glycoprotein (VSV-G) has been widely used for pseudotyping retroviral, lentiviral, and artificial viral vectors. The objective of this study was to establish a potential approach for large-scale production of VSV-G. To this end, VSV-G was cloned with an N-terminal His-tag into Pichia pastoris expression vector pPIC3.5K. Three clones (Mut^s) containing the VSV-G expression cassette were identified by PCR. All clones proliferated normally in expansion medium, whereas the proliferation was reduced significantly under induction conditions. VSV-G protein was detected in cell lysates by western blot analysis, and the highest expression level was observed at 96 h post induction. VSV-G could also be obtained from the condition medium of yeast protoplasts. Furthermore, VSV-G could be incorporated into Ad293 cells and was able to induce cell fusion, leading to the transfer of cytoplasmic protein. Finally, VSV-G-mediated DNA transfection was assayed by flow cytometry and luciferase measurement. Incubation of VSV-G lysate with the pGL3-control DNA complex increased the luciferase activity in Ad293 and HeLa cells by about 3-fold. Likewise, incubation of VSV-G lysate with the pCMV-DsRed DNA complex improved the transfection efficiency into Ad293 by 10% and into HeLa cells by about 1-fold. In conclusion, these results demonstrate that VSV-G could be produced from P. pastoris with biofunctionalities, demonstrating that large-scale production of the viral glycoprotein is feasible.

The Antihydrolytic Effect and Properties of Crosslinked Polyurethane Containing Natural Dimer Fatty Acids Building Blocks

Xin Liu,Kai Xu,Huan Liu,Hualun Cai,Zien Fu,Ying Guo,Mingcai Chen 한국고분자학회 2012 Macromolecular Research Vol.20 No.6

Four series of cross-linked polyurethanes with polyester polyols as soft segments were prepared by selfcuring of isocyanate-terminated polyurethanes. Polyester polyols herein were derived from different dimer fatty acids weight content, such as dicarboxylic acid leading to various hydrophobicity and steric hindrance. The structure of cross-linked polyurethane was characterized by Fourier transform infrared spectroscopy (FTIR). The surface properties and mechanical and thermal properties of cross-linked polyurethane were investigated using contact meter, tensile tester, differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA). The results indicated that with increasing dimer fatty acid weight content from 0% to 100%, the cross-linked polyurethanes exhibited increased hydrophobicity (water contact angle increased from 91o to 96o), improved water resistance (water uptake decreased from 1.41% to 0.34%), toluene resistance (weight loss decreased from 33.2% to 22.7%), and hydrolytic resistance (weight loss decreased from 0.60% to 0.27%), enhanced mechanical properties (tensile strength increased from 1.3 to 4.8 MPa), and thermal stability (decomposition temperature of 50% weight loss increased from 425 to 462 oC).

Anti-proliferation Effects of Interferon-gamma on Gastric Cancer Cells

Zhao, Ying-Hui,Wang, Tao,Yu, Guang-Fu,Zhuang, Dong-Ming,Zhang, Zhong,Zhang, Hong-Xin,Zhao, Da-Peng,Yu, Ai-Lian Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.9

IFN-${\gamma}$ plays an indirect anti-cancer role through the immune system but may have direct negative effects on cancer cells. It regulates the viability of gastric cancer cells, so we examined whether it affects their proliferation and how that might be brought about. We exposed AGS, HGC-27 and GES-1 gastric cancer cell lines to IFN-${\gamma}$ and found significantly reduced colony formation ability. Flow cytometry revealed no effect of IFN-${\gamma}$ on apoptosis of cell lines and no effect on cell aging as assessed by ${\beta}$-gal staining. Microarray assay revealed that IFN-${\gamma}$ changed the mRNA expression of genes related to the cell cycle and cell proliferation and migration, as well as chemokines and chemokine receptors, and immunity-related genes. Finally, flow cytometry revealed that IFN-${\gamma}$ arrested the cells in the G1/S phase. IFN-${\gamma}$ may slow proliferation of some gastric cancer cells by affecting the cell cycle to play a negative role in the development of gastric cancer.

Collateral Flow in Magnetic Resonance Angiography: Prognostic Value for Vertebrobasilar Stenosis With Stroke Recurrence

Long Yan,Ying Yu,Kaijiang Kang,Zhikai Hou,Min Wan,Weilun Fu,Rongrong Cui,Yongjun Wang,Zhongrong Miao,Xin Lou,Ning Ma 대한신경과학회 2022 Journal of Clinical Neurology Vol.18 No.5

Background and Purpose Intracranial vertebrobasilar atherosclerotic stenosis (IVBAS) is a major cause of posterior circulation stroke. Some patients suffer from stroke recurrence despite receiving medical treatment. This study aimed to determine the prognostic value of a new score for the posterior communicating artery and the P1 segment of the posterior cerebral artery (PCoA-P1) for predicting stroke recurrence in IVBAS. Methods We retrospectively enrolled patients with severe IVBAS (70%–99%). According to the number of stroke recurrences, patients were divided into no-recurrence, single-recurrence, and multiple-recurrences groups. We developed a new 5-point grading scale, with the PCoA-P1 score ranging from 0 to 4 based on magnetic resonance angiography, in which primary collaterals were dichotomized into good (2–4 points) and poor (0 or 1 point). Stroke recurrences after the index stroke were recorded. Patients who did not experience stroke recurrence were compared with those who experienced single or multiple stroke recurrences. Results From January 2012 to December 2019, 176 patients were enrolled, of which 116 (65.9%) had no stroke recurrence, 35 (19.9%) had a single stroke recurrence, and 25 (14.2%) had multiple stroke recurrences. Patients with single stroke recurrence (odds ratio [OR]= 4.134, 95% confidence interval [CI]=1.822–9.380, p=0.001) and multiple stroke recurrences (OR=6.894, 95% CI=2.489–19.092, p<0.001) were more likely to have poor primary collaterals than those with no stroke recurrence. Conclusions The new PCoA-P1 score appears to provide improve predictions of stroke recurrence in patients with IVBAS

BRCA1 Gene Exon 11 Mutations in Uighur and Han Women with Early-onset Sporadic Breast Cancer in the Northwest Region of China

Cao, Yu-Wen,Fu, Xin-Ge,Wan, Guo-Xing,Yu, Shi-Ying,Cui, Xiao-Bin,Li, Li,Jiang, Jin-Fang,Zheng, Yu-Qin,Zhang, Wen-Jie,Li, Feng Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.11

The prevalence of BRCA1 gene mutations in breast cancer differs between diverse ethnic groups. Relatively little information is known about patterns of BRCA1 mutations in early-onset breast cancer in women of Uighur or Han descent, the major ethnic populations of the Xinjiang region in China. The aim of this study was to identify BRCA1 mutations in Uighur and Han patients with early-onset (age <35 years), and sporadic breast cancer for genetic predisposition to breast cancer. For detection of BRCA1 mutations, we used a polymerase chain reaction single-stranded conformation polymorphism approach, followed by direct DNA sequencing in 22 Uighur and 13 Han women with early-onset sporadic breast cancer, and 32 women with benign breast diseases. The prevalence of BRCA1 mutations in this population was 22.9% (8/35) among early-onset sporadic breast cancer cases. Of these, 31.8% (7/22) of Uighur patients and 7.69% (1/13) of Han patients were found to have BRCA1 mutations. In 7 Uighur patients with BRCA1 mutations, there were 11 unique sequence alterations in the BRCA1 gene, including 4 clearly disease-associated mutations on exon 11 and 3 variants of uncertain clinical significance on exon 11, meanwhile 4 neutral variants on intron 20 or 2. None of the 11 BRCA1 mutations identified have been previously reported in the Breast Cancer Information Core database. These findings reflect the prevalence of BRCA1 mutations in Uighur women with early-onset and sporadic breast cancer, which will allow for provision of appropriate genetic counseling and treatment for Uighur patients in the Xinjiang region.

20(S)-Protopanaxadiol Induces Human Breast Cancer MCF-7 Apoptosis through a Caspase-Mediated Pathway

Zhang, Hong,Xu, Hua-Li,Fu, Wen-Wen,Xin, Ying,Li, Mao-Wei,Wang, Shuai-Jun,Yu, Xiao-Feng,Sui, Da-Yun Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.18

20(S)-Protopanaxadiol (PPD), a ginsenoside isolated from Pananx quinquefolium L., has been shown to inhibit growth and proliferation in several cancer cell lines. The aim of this study was to evaluate its anticancer activity in human breast cancer cells. MCF-7 cells were incubated with different concentrations of 20(S)-PPD and cytotoxicity was evaluated by MTT assay. Occurrence of apoptosis was detected by DAPI and Annexin V-FITC/PI double staining. Mitochondrial membrane potential was measured with Rhodamine 123. The Bcl-2 and Bax expression were determined by Western blot analysis. Caspase activity was measured by colorimetric assay. 20(S)-PPD dose-dependently inhibited cell proliferation in MCF-7 cells, with an $IC_{50}$ value of $33.3{\mu}M$ at 24h. MCF-7 cells treated with 20(S)-PPD presented typical apoptosis, as observed by morphological analysis in cell stained with DAPI. The percentages of annexin V-FITC positive cells were 8.92%, 17.8%, 24.5% and 30.5% in MCF-7 cells treated with 0, 15, 30 and $60{\mu}M$ of 20(S)-PPD, respectively. Moreover, 20(S)-PPD could induce mitochondrial membrane potential loss, up-regulate Bax expression and down-regulate Bcl-2 expression. These events paralleled activation of caspase-9, -3 and PARP cleavage. Apoptosis induced by 20(S)-PPD was blocked by z-VAD-fmk, a pan-caspase inhibitor, suggesting induction of caspase-mediated apoptotic cell death. In conclusion, the 20(S)-PPD investigated is able to inhibit cell proliferation and to induce cancer cell death by a caspase-mediated apoptosis pathway.

Non-Doped Organic Light-Emitting Diodes with Saturated Red Emission

Fei Xiao,Bing-xian Shao,Huan-rong Wu,Hui-ying Fu,Xiao-yuan Hou,Xin-dong Gao,Yi-qiang Zhan 한국물리학회 2007 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.50 No.2

Non-doped organic light-emitting diodes with saturated red emission were fabricated using 4-(2-(3,3-dicyanomethylene-5,5-dimethyl-1-cyclohexylidene)vinyl)phenyldi(1-naphthyl)amine (DNP-2CN) or 4-(2-(3,3-dicyanomethylene-5,5-dimethyl-1-cyclohexylidene)vinyl)phenyl(1-naphthyl)phe- nylamine (DPN-2CN) as the emitting layer. Different electron-transporting materials, tris(8-hydroxylquinoline) aluminum (Alq$_3$), 2,2',2''-(1,3,5-phenylene)tris[1-phenyl-1$H$-benzimidazole] (TPBI) and 2-(4-biphenyl)-5-(4-tert-butylphenyl)-1,3,4-oxadiazole (PBD), were introduced into the devices for examining their energy level compatibility of DNP-2CN or DPN-2CN. The device with a structure of ITO/ NPB/ DNP-2CN/ BCP/ Alq$_3$/ LiF/ Al showed red emission with $\lambda_{max}$ at 670 nm (CIE coordinates: $x$ = 0.66, $y$ = 0.33) and a high luminance of 438 cd m$^{-2}$ at a driving voltage of 12 V. The device with a structure of ITO/ NPB/ DPN-2CN/ BCP/ Alq$_3$/ LiF/ Al showed a high brightness of 225 cd m$^{-2}$ at a driving voltage of 12 V with $\lambda_{max}$ at 674 nm (CIE coordinates: $x$ = 0.65, $y$ = 0.33).