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- 저자 : ( Tadashi Toyama ) (검색결과 5 건)
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Chang, Young-Cheol,Toyama, Tadashi,Jung, Kweon,Kikuchi, Shitaro Korean Society of Environmental Health 2006 한국환경보건학회지 Vol.32 No.6
The objective of this paper is to compare the applicability of assimilable organic carbon (AOC) or biodegradable dissolved organic carbon (BDOC) for quantifying biodegradable organic material (BOM) and bio-stability in distribution systems for a variety of finished waters. The study the data is derived from was part of an AWWARF and Tampa Bay Water tailored collaboration project to determine the effect of blending different waters on distribution system water quality. Seven different finished waters were produced from surface, ground, or brackish water on site and fed 18 independent pilot distribution systems (PDSs), either as single finished water or as a blend of several finished waters. AOC and BDOC have often been used as indicators of bacterial regrowth potential in distribution systems. In this study, AOC was the more useful assay of the two for the BOM concentrations observed in the PDSs. BDOC did not distinguish BOM while AOC did at the low BOM levels from many of the advanced treatments (e.g. RO, $O^3/BAC$). AOC in contrast allowed much more meaningful calculations of the consumption or production of AOC as the blends passed through the PDSs even for very low BOM blends. In addition, meaningful trends corresponding to changes in heterophic plate count (HPC) were observed for AOC but not for BDOC. Moreover, AOC stability was associated with waters produced from advanced membrane treatment.
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Chang, Young-Cheol,Toyama, Tadashi,Kikuchi, Shintaro Korean Society for Environmental Sanitary Engineer 2008 대한환경위생공학회지 Vol.13 No.2
The tetrachloroethylene (PCE) dehalogenase of Clostridium bifermentans DPH-1 (a halorespiring organism) was purified, cloned, and sequenced. This enzyme is a homodimer with a molecular mass of ca. 70 kDa and exhibits dehalogenation of dichloroethylene isomers along with PCE and trichloroethylene (TCE). Broad range of substrate specificity for chlorinated aliphatic compounds (PCE, TCE, cis-1,2-dichloroethylene, trans-1,2-dichloroethylene, 1,1-dichloroethylene, 1,2-dichloropropene, and 1,1,2-trichloroethane) for this enzyme was also observed. A mixture of propyl iodide and titanium citrate caused a light-reversible inhibition of enzymatic activity suggesting the involvement of a corrinoid cofactor. A partial sequence (81 bp) of the encoding gene for PCE dehalogenase was amplified and sequenced with degenerateprimers designed from the N-terminal sequence (27 amino acid residues). Southern analysis of C. bifermentans genomic DNA using the polymerase chain reaction product as a probe revealed restriction fragment bands. A 5.0 kb ClaI fragment, harboring the relevant gene (designated pceC) was cloned (pDEHAL5) and the complete nucleotide sequence of pceC was determined. The gene showed homology mainly with microbial membrane proteins and no homology with any known dehalogenase, suggesting a distinct PCE dehalogenase. So, C. bifermentans could play some important role in the initial breakdown of PCE and other chlorinated aliphatic compounds in sites contaminated with mixtures of halogenated substances.
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Yong-Cheol Chang(서평자),Tadashi Toyama(서평자),Shintaro Kikuchi(서평자) 대한환경위생공학회 2008 대한환경위생공학회지 Vol.23 No.2
The tetrachloroethylene (PCE) dehalogenase of Clostridium bifermentans DPH-1 (a halorespiring organism) was purified, cloned, and sequenced. This enzyme is a homodimer with a molecular mass of ca. 70 kDa and exhibits dehalogenation of dichloroethylene isomers along with PCE and trichloroethylene (TCE). Broad range of substrate specificity for chlorinated aliphatic compounds (PCE, TCE, cis-1,2-dichloroethylene, trans-1,2-dichloroethylene, 1,1-dichloroethylene. 1,2-dichloropropene, and 1,1,2-trichloroethane) for this enzyme was also observed. A mixture of propyl iodide and titanium citrate caused a light-reversible inhibition of enzymatic activity suggesting the involvement of a corrinoid cofactor. A partial sequence (81 bp) of the encoding gene for PCE dehalogenase was amplified and sequenced with degenerateprimers designed from the N-terrninal sequence (27 amino acid residues). Southern analysis of C. biiermentsns genomic DNA using the polymerase chain reaction product as a probe revealed restriction fragment bands. A 5.0 kb ClaI fragment, harboring the relevant gene (designated pceC) was cloned (pDEHAL5) and the complete nucleotide sequence of pceC was determined. The gene showed homology mainly with microbial membrane proteins and no homology with any known dehalogenase, suggesting a distinct PCE dehalogenase. So, C. bitermentsns could play some important role in the initial breakdown of PCE and other chlorinated aliphatic compounds in sites contaminated with mixtures of halogenated substances.
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Bioremediation of Crude Oil by White Rot Fungi Polyporus sp. S133
( Kristanti Risky Ayu ),( Tony Hadibarata ),( Tadashi Toyama ),( Yasuhiro Tanaka ),( Kazuhiro Mori ) 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.9
The bioremediation potential of crude oil by Polyporus sp. S133 pre-grown in wood meal was investigated in two separate experiment trials; liquid medium and soil. The effect of three nutrients (glucose, polypeptone, and wood meal), oxygen flow, and some absorbent on the efficiency of the process was also evaluated. Degradation of crude oil in soil was significantly increased with an addition of oxygen flow and some absorbent (kapok and pulp). The highest degradation rate of crude oil was 93% in the soil with an addition of 10% kapok. The present study clearly demonstrates that, if suitably developed, Polyporus sp. S133 could be used to remediate soil contaminated with crude oil.
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Tomoyuki Hayashi,Yoshiro Asahina,Yasuhito Takeda,Masaki Miyazawa,Hajime Takatori,Hidenori Kido,Jun Seishima,Noriho Iida,Kazuya Kitamura,Takeshi Terashima,Sakae Miyagi,Tadashi Toyama,Eishiro Mizukoshi 대한소화기내시경학회 2023 Clinical Endoscopy Vol.56 No.5
Background/Aims: The necessity for pharyngeal anesthesia during upper gastrointestinal endoscopy is controversial. This study aimedto compare the observation ability with and without pharyngeal anesthesia under midazolam sedation. Methods: This prospective, single-blinded, randomized study included 500 patients who underwent transoral upper gastrointestinalendoscopy under intravenous midazolam sedation. Patients were randomly allocated to pharyngeal anesthesia: PA+ or PA– groups (250patients/group). The endoscopists obtained 10 images of the oropharynx and hypopharynx. The primary outcome was the non-inferiorityof the PA- group in terms of the pharyngeal observation success rate. Results: The pharyngeal observation success rates in the pharyngeal anesthesia with and without (PA+ and PA–) groups were 84.0%and 72.0%, respectively. The PA– group was inferior (p=0.707, non-inferiority) to the PA+ group in terms of observable parts (8.33 vs. 8.86, p=0.006), time (67.2 vs. 58.2 seconds, p=0.001), and pain (1.21±2.37 vs. 0.68±1.78, p=0.004, 0–10 point visual analog scale). Suitablequality images of the posterior wall of the oropharynx, vocal fold, and pyriform sinus were inferior in the PA– group. Subgroupanalysis showed a higher sedation level (Ramsay score ≥5) with almost no differences in the pharyngeal observation success rate betweenthe groups. Conclusions: Non-pharyngeal anesthesia showed no non-inferiority in pharyngeal observation ability. Pharyngeal anesthesia may improvepharyngeal observation ability in the hypopharynx and reduce pain. However, deeper anesthesia may reduce this difference.
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