C-reactive protein accelerates DRP1-mediated mitochondrial fission by modulating ERK1/2-YAP signaling in cardiomyocytes

( Suyeon Jin ),( Chan Joo Lee ),( Gibbeum Lim ),( Sungha Park ),( Sang-hak Lee ),( Ji Hyung Chung ),( Jaewon Oh ),( Seok-min Kang ) 생화학분자생물학회 2023 BMB Reports Vol.56 No.12

C-reactive protein (CRP) is an inflammatory marker and risk factor for atherosclerosis and cardiovascular diseases. However, the mechanism through which CRP induces myocardial damage remains unclear. This study aimed to determine how CRP damages cardiomyocytes via the change of mitochondrial dynamics and whether survivin, an anti-apoptotic protein, exerts a cardioprotective effect in this process. We treated H9c2 cardiomyocytes with CRP and found increased intracellular ROS production and shortened mitochondrial length. CRP treatment phosphorylated ERK1/2 and promoted increased expression, phosphorylation, and translocation of DRP1, a mitochondrial fission-related protein, from the cytoplasm to the mitochondria. The expression of mitophagy proteins PINK1 and PARK2 was also increased by CRP. YAP, a transcriptional regulator of PINK1 and PARK2, was also increased by CRP. Knockdown of YAP prevented CRP-induced increases in DRP1, PINK1, and PARK2. Furthermore, CRP-induced changes in the expression of DRP1 and increases in YAP, PINK1, and PARK2 were inhibited by ERK1/2 inhibition, suggesting that ERK1/2 signaling is involved in CRP-induced mitochondrial fission. We treated H9c2 cardiomyocytes with a recombinant TAT-survivin protein before CRP treatment, which reduced CRP-induced ROS accumulation and reduced mitochondrial fission. CRP-induced activation of ERK1/2 and increases in the expression and activity of YAP and its downstream mitochondrial proteins were inhibited by TAT-survivin. This study shows that mitochondrial fission occurs during CRPinduced cardiomyocyte damage and that the ERK1/2-YAP axis is involved in this process, and identifies that survivin alters these mechanisms to prevent CRP-induced mitochondrial damage. [BMB Reports 2023; 56(12): 663-668]

Expert panel consensus recommendations for home blood pressure monitoring in Asia: the Hope Asia Network

Park, Sungha,Buranakitjaroen, Peera,Chen, Chen-Huan,Chia, Yook-Chin,Divinagracia, Romeo,Hoshide, Satoshi,Shin, Jinho,Siddique, Saulat,Sison, Jorge,Soenarta, Arieska Ann,Sogunuru, Guru Prasad,Tay, Jam Nature Publishing Group UK 2018 Journal of human hypertension Vol.32 No.4

<P>Hypertension is the leading cause of mortality throughout Asia. Home blood pressure monitoring has the potential to improve hypertension control and is a useful adjunct to conventional office blood pressure measurements due to its diagnostic accuracy and prognostic value in predicting cardiovascular outcomes. At present, there are no region-specific guidelines addressing the use of home blood pressure monitoring in Asia. Therefore, an expert panel was convened to address the use of home blood pressure monitoring and develop key recommendations to help guide clinical practice throughout the Asia region. The resulting recommendations support the use of home blood pressure monitoring with a validated device as an accurate adjunct for diagnosing hypertension and predicting cardiovascular outcome. Diagnosis and treatment of hypertension should still be guided by conventional office/clinic blood pressure measurements. The expert panel encourages the incorporation of home blood pressure monitoring into local clinical guidelines and offers practical recommendations to ensure continuity of care where a validated home blood pressure device is not available.</P>

[Hepato-Biliary and Pancreas] The Effect of Donor against Recipient One-way HLA Mismatch on Liver Transplantation Outcomes: An Analysis of Korean Organ Transplantation Registry (KOTRY) Database

Sunghae Park 대한외과학회 2022 대한외과학회 학술대회 초록집 Vol.2022 No.5

Cost-effective and time-saving three-dimensional (3-D) printing protocol of intra-abdominal cavity of liver transplantation recipient to prevent large-for-size syndrome

Sunghae PARK,Gyu-Seong CHOI,Jong Man KIM,Sanghoon LEE,Jae-Won JOH,Jinsoo RHU 한국간담췌외과학회 2022 Annals of hepato-biliary-pancreatic surgery Vol.26 No.-

Association of central hemodynamics with estimated 24-h urinary sodium in patients with hypertension

Park, Sungha,Park, Jeong B.,Lakatta, Edward G. Lippincott Williams Wilkins, Inc. 2011 Journal of Hypertension Vol.29 No.8

OBJECTIVE: High salt intake is known to be the most pivotal environmental factor in the pathogenesis of hypertension. However, the association of high sodium intake with central hemodynamics in hypertensive individuals has not been well defined. Here, we determined the association of estimated 24-h urine sodium and potassium excretion estimated from a spot urine analysis with parameters of central pulse wave analysis in 515 hypertensive individuals. METHODS: Fasting spot urine samples were obtained in the early morning after the first void, and estimated 24-h urine sodium and potassium excretion were estimated from measurement of urine sodium, potassium and creatinine. Central hemodynamics and arterial stiffness parameters were assessed via pulse wave analysis of the radial artery. RESULTS: The estimated 24-h sodium and potassium excretion values were 150 ± 40 and 49 ± 10 mEq, respectively. There was a step-wise decrease in pulse pressure amplification with increasing estimated 24-h urine sodium excretion. Multiple linear regression analyses revealed that both estimated 24-h urine sodium excretion and sodium/potassium ratio were independently associated with increases in central pulse pressure, augmented aortic pressure and augmentation index and were inversely associated with pulse pressure amplification. CONCLUSION: The estimated 24-h urinary sodium excretion is independently associated with central hemodynamics. This may provide the basis for prospective interventional studies of epidemiologic scale to determine the potential beneficial effects of reduced salt consumption on central hemodynamics.

Liver Resection in Elderly HCC Patients (≥ 75 years) Is Safe

( Sunghae Park ),( Young Ju Oh ),( Sang Oh Yun ),( Manuel Lim ),( Ji Eun Jeong ),( Eun Sung Jeong ),( Jaehun Yang ),( Jinsoo Rhu ),( Gyu-seong Choi ),( Jong Man Kim ),( Jae-won Joh ) 대한간학회 2021 춘·추계 학술대회 (KASL) Vol.2021 No.1

How is Youth`s Emotion Cultivated in Dance Education? : An Exploratory Study

( Sunghae Park ) 한국체육학회 2016 국제스포츠과학 학술대회 Vol.2016 No.1

Purpose: As emotional problems of youth are getting serious, there has been a growing interest in developing youth’s emotion. However, most of research and programs have focused on the cognitive aspects of emotion and dealt with special populations of youth with behavior problems. In this context, this study is an exploratory study investigating the cultivation of emotion in general youth populations. The purposes of this study are to identify positive emotional experiences of youth in dance education and explore the factors that contribute to the cultivation of youth’s emotion. Research questions are as follows: (a) What are the positive emotional experiences of youth in dance education?, (b) What are the factors that have a positive impact on youth’s emotion? Method: Qualitative research method was conducted for this study. Participants were 16 to 22 years old students (n=20, 9 males and 11 females) who were engaged in dance education lessons. Data were collected by open-ended questionnaires, non-participant observations, and in-depth interviews. Data were analyzed inductively and trustworthiness of data was enhanced through member checks and peer debriefing. Result: Two aspects of youth’s emotional experiences . personal and interpersonal emotions - were classified. The personal emotion included an immediate and instinctive sense of feelings (e.g. pleasure, excitement, and happiness, etc.) and complicated combination of those feelings (e.g. a sense of fulfillment, confidence, and self-examination, etc.). The interpersonal emotion was shown in terms of various layers from empathy with others to a sense of consideration, faith, and duty toward others. There were three main factors promoting youth’s emotional cultivation. Diverse narrative contents, songs, movies, readings, writings, and discussions, with dance techniques enriched youth’s emotional experiences. In addition, appropriate teachers’ indirect teaching behavior and positive interactions with classmates played a crucial role in contributing to the cultivation of youth’s emotion. Conclusion: Dance education can be seen as essential for cultivating youth’s emotion. Furthermore, youth’s emotional cultivation (e.g. caring, cooperation, and responsibility) is closed to character development. For the further research, it will be needed to study the roles of teachers and foundations of dance education programs in order to maximize positive youth’s emotional experiences.

The influence of serum aldosterone and the aldosterone–renin ratio on pulse wave velocity in hypertensive patients

Park, Sungha,Kim, Jin-Bae,Shim, Chi Young,Ko, Young-Guk,Choi, Donghoon,Jang, Yangsoo,Chung, Namsik Lippincott Williams Wilkins, Inc. 2007 Journal of Hypertension Vol.25 No.6

OBJECTIVE: The aldosterone–renin ratio (ARR) is widely used to screen for primary aldosteronism, and may reflect a relative excess of aldosterone secretion compared with renin secretion. Excess aldosterone may have a detrimental effect on vascular stiffness. We therefore hypothesized that ARR and aldosterone are independently correlated with pulse wave velocity (PWV) in hypertensive patients. METHODS: The study consisted of 438 hypertensive patients. Serum aldosterone and plasma renin activity were measured in a sitting position using standard techniques. The PWV was determined by measuring the heart to femoral PWV (hfPWV) and brachial to ankle PWV with a VP-2000 pulse wave unit. RESULTS: Group 1 was defined as patients with ARR of at least 20 (n = 53) with serum aldosterone ≥ 12 ng/dl, while the remainder comprised group 2. Comparisons between the two groups reveal group 1 tended to have higher age, significantly higher proportion of women and higher systolic/diastolic blood pressure. Patients in group 1 also had a significantly higher index of central arterial stiffness (hfPWV 1048 ± 202 vs 978 ± 182, P = 0.010) compared with group 2. Multiple linear regression revealed that aldosterone, but not the ARR, is significantly associated with hfPWV but not brachial to ankle PWV, after controlling for age, systolic blood pressure and heart rate at the time of PWV measurement, body mass index, gender, low-density lipoprotein-cholesterol, triglyceride, high-density lipoprotein-cholesterol, blood pressure medication and statins. CONCLUSIONS: Serum aldosterone is significantly associated with central aortic PWV in hypertensive patients. The results demonstrate a possible role for aldosterone in developing central aortic stiffness and increased PWV in hypertensive patients.

Genetic polymorphism in the pregnancy-associated plasma protein-A associated with acute myocardial infarction

Park, Sungha,Youn, Jong-Chan,Shin, Dong-Jik,Park, Chan-Mi,Kim, Jung-Sun,Ko, Young-Guk,Choi, Donghoon,Ha, Jong-Won,Jang, Yangsoo,Chung, Namsik Lippincott Williams Wilkins, Inc. 2007 Coronary artery disease Vol.18 No.6

BACKGROUND: Pregnancy-associated plasma protein-A (PAPP-A) is a high-molecular-weight, zinc-binding matrix metalloproteinase that is known to be abundantly expressed in ruptured plaques. Previous studies have shown PAPP-A to be a significant marker of plaque instability and cardiovascular events in patients with acute coronary syndromes. Because the activity of PAPP-A may be modulated by genetic variants in the PAPP-A genes, we tried to determine the association of PAPP-A gene with acute myocardial infarction (AMI). METHODS: We analyzed four single nucleotide polymorphisms (SNPs) of PAPP-A gene variants and seven other polymorphisms of cytokine genes that have been reported to have functional significance (RANTES G-403A, MCP1 G-2518A, CRP A2147G, CRP G-717A, AGER G557A, LTA T26A, IL-6 G-572C) for possible association with AMI in 170 unrelated AMI patients and unrelated age-matched controls, respectively. RESULTS: The average age of the study population was 62.2±11.4 years in AMI patients and 62.6±10.4 years in healthy controls. Multiple logistic regression analysis with risk factors such as age, male sex, smoking, hypertension, diabetes mellitus, and dyslipidemia revealed the PAPP-A IVS6+95 C allele to be associated with an increased risk of AMI (dominancy: odds ratio, 2.13; 95% confidence interval, 1.12–4.07; P=0.022; codominancy: odds ratio, 1.89; 95% confidence interval, 1.14–3.16; P=0.015). CONCLUSIONS: We found, for the first time, that PAPP-A IVS6+95 C allele is an independent risk factor for AMI even after adjustment for traditional risk factors. The determination of such genotype contributing to AMI could provide a new tool for identifying high-risk individuals.

Resistant hypertension: consensus document from the Korean society of hypertension

Park Sungha,Shin Jinho,임상현,김광일,Kim Hack-Lyoung,Kim Hyeon Chang,Lee Eun Mi,Lee Jang Hoon,Ahn Shin Young,Cho Eun Joo,Kim Ju Han,Kang Hee-Taik,Lee Hae-Young,Lee Sunki,Kim Woohyeun,Park Jong-Moo 대한고혈압학회 2023 Clinical Hypertension Vol.29 No.-

Although reports vary, the prevalence of true resistant hypertension and apparent treatment-resistant hypertension (aTRH) has been reported to be 10.3% and 14.7%, respectively. As there is a rapid increase in the prevalence of obesity, chronic kidney disease, and diabetes mellitus, factors that are associated with resistant hypertension, the prevalence of resistant hypertension is expected to rise as well. Frequently, patients with aTRH have pseudoresistant hypertension [aTRH due to white-coat uncontrolled hypertension (WUCH), drug underdosing, poor adherence, and inaccurate office blood pressure (BP) measurements]. As the prevalence of WUCH is high among patients with aTRH, the use of out-of-office BP measurements, both ambulatory blood pressure monitoring (ABPM) and home blood pressure monitoring (HBPM), is essential to exclude WUCH. Non-adherence is especially problematic, and methods to assess adherence remain limited and often not clinically feasible. Therefore, the use of HBPM and higher utilization of singlepill fixed-dose combination treatments should be emphasized to improve drug adherence. In addition, primary aldosteronism and symptomatic obstructive sleep apnea are quite common in patients with hypertension and more so in patients with resistant hypertension. Screening for these diseases is essential, as the treatment of these secondary causes may help control BP in patients who are otherwise difficult to treat. Finally, a proper drug regimen combined with lifestyle modifications is essential to control BP in these patients.