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선천성 거대결장증 진단에 필요한 조직화학염색 : Rapid Acetylcholinesterase Staining
설지영,김제룡,김진만,장일성 충남대학교 의학연구소 2001 충남의대잡지 Vol.28 No.2
Hirschsprung's disease is a congenital absence of ganglion cells in the Meissner's and Auerbach's plexuses of the colon. The definitive diagnosis of the disease can only be based on histologic examination of bowel wall biopsies demonstrating aganglionosis. To distinguish Hirschsprung's disease from other causes of contipation and megacolon, it is helpful to use histologic examination with routine hematoxylin and eosin (H&E) staining and acetylcholinesterase (AChE) special histochemical staining techniques. With H&E staining, the difficulties may arise in the hypoganglionic area of the transition zone, or in hypoganglionosis or neuronal intestinal dyspiasia. The AChE technique provides reliable identification of ganglion cells by staining nerve filaments in close proximity to the ganglion cells. However, the conventional AChE technique takes about 2 hours for the examination of specimens, so it is not suitable for intraoperative examination. Using a rapid AChE histochemical staining technique, it is possible to produce staining of cholinergic fibers in 20 minutes. It should enable the surgeon to use it selectively during operation and to support or refute the H&E findings.
Seung Ryong Park,Seung Hyun Sul,Sang Youn Lee 한국유통과학회 2017 KODISA ICBE (International Conference on Business Vol.2017 No.-
Mac Donald, large business of American fast food, had recently opened number 1 branch shop at Ho Chi Minh City, Vietnam to pay attention to Vietnamese franchise market again. Franchise business in the world thinks that opening of branch shop of Mac Donald, representative franchise business, indicates Mac Donald's paying attention of the Vietnamese market to enter new market and to be valuable to domestic franchise business as well. The United States introduced franchise business franchise business system for the first time after the World War Two and then Japan introduced it. South Korea introduced franchise business system in 1979 to develop it and to settle down intellectual properties according to contract culture and to open shop for a short period by using bulk purchase, joint distribution, joint marketing and analysis upon commercial area and location being different from the United States and to raise competitiveness and profits at early stage of brand launching and to be an area of domestic industry for relatively short time. These days, domestic franchise businesses have managed Korean system owing to success of Korean style franchise system to enter foreign market based on experience and know how of Korean style franchise system that the Ministry of Industry, Commerce and Resources, SEMAS and the Association of Franchise Industry of Korea have supported. Korean franchise businesses' entry into overseas market requires wide range of market survey, and time, efforts and money for preliminary preparation to have risks. After investigating culture, customs, living life levels and associated laws and regulations, Korean franchise businesses are allowed to make decision of entry into overseas market. Korean franchise enterprises' entry into the Vietnamese market is still in beginning stage. The Vietnamese market has recorded the highest economic growth among South East Asian counties owing to economic development plan of door opening policy (Đổi m