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        Application of organic-inorganic hybrid composite particle for removal of heavy metal ions from aqueous solution and its toxicity evaluation

        Lee, Soonjae,Kim, Nahae,Cho, Seulki,Ryu, Jae-Chun,Cho, Yoon,Park, Jeong-Ann,Lee, Sang-Hyup,Kim, Juyoung,Choi, Jae-Woo Elsevier 2017 European polymer journal Vol.95 No.-

        <P><B>Abstract</B></P> <P>In this study, we present new organic–inorganic (O-I) hybrid composite particles and their possible application asan adsorbent for removal of various inorganic heavy metal from aqueous phase. The O-I hybrid nanoparticles were first prepared by copolymerization of (3-aminopropyl) triethoxysilane (APTES) and alkoxysilane-functionalized amphiphilic polymer (APAS) precursors which have hydrophobic polypropylene oxide segment and hydrophilic polyethylene oxide segment at the same backbones where triethoxysilane groups are chemically bonded with hydrophobic segment. The O-I hybrid composite particles consisting of silica nanoparticles chemically interconnected by O-I hybrid nanoparticles were prepared using the 2nd hydrolysis-condensation reaction. The characteristics of the hybrid particle thus developed were confirmed by FT-IR (Fourier transform infrared) spectroscopy, <SUP>29</SUP>Si-NMR (nuclear magnetic resonance) spectroscopy, EA (Elemental analysis), TGA (thermogravimetric analysis), SEM (scanning electron microscopy), and cryo-TEM (cryogenic transmission electron microscopy). The O-I hybrid particles exhibited removal of cations (Ni, Cu, Zn, Cd and Pb) from aqueous solution with a considerably higher sorption capacity for Pb than for other cations. The heavy metal removal occurred instantaneously with pH increase. A step wise application tests were conducted to distinguish between heavy metal precipitation at a high pH and adsorption by the developed material. Cytotoxicity tests demonstrated that the developed adsorbent has a weak cytotoxic effect on four different types of human cells.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Amphiphilic polymer precursor is conjugated onto silica nanoparticles. </LI> <LI> The composite removed various heavy metal ions from aqueous solution. </LI> <LI> The composite is a promising adsorbent for hydrophilic and/or hydrophobic pollutants. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

      • KCI등재

        Analysis of Inflammatory Cytokines from the Cecum and Proximal Colon of Mice Infected with Enterotoxigenic Bacteroides fragilis

        Soonjae Hwang,Min Ho Lee,Sun-Yeong Gwon,Seunghyung Lee,Dongju Jung,Ki-Jong Rhee 대한의생명과학회 2013 Biomedical Science Letters Vol.19 No.2

        Enterotoxigenic Bacteroides fragilis (ETBF) causes inflammatory diarrhea in humans and animals and is also implicated in colorectal cancer. ETBF-infected mice exhibit a prominent large intestinal inflammation characterized by neutrophil infiltration and induction of the Th17 response. In this study, we examined differences in the secreted cytokine profile of the cecum and proximal colon of ETBF-infected mice using an antibody array. Of the cytokines examined, we found that the cecal tissues from ETBF-infected mice secreted elevated levels of G-CSF, IL-6, IL-17 and LIX compared to non-toxigenic Bacteroides fragilis (NTBF) and Mock infected mice. The proximal colon tissues from ETBF-infected mice secreted higher levels of G-CSF, IL-6, KC, LIX, MIP-1g and MCP-1. This study demonstrates that the cecum and colon should be considered separately when assays are used to determine immune responsiveness to enteric infections.

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      • KCI등재

        유아특수교사양성기관의 학교현장실습 관련 국내 연구동향 분석

        이순자 ( Lee Soonja ),이정은 ( Lee Chung Eun ) 한국유아특수교육학회 2023 유아특수교육연구 Vol.23 No.3

        교원양성대학의 예비교사 교육에 있어 학교현장실습은 역량과 전문성을 키우는 데 있어 직접적인 기회를 제공하는 과정이다. 학교 현장실습을 강화하기 위한 연구가 그동안 이루어져왔으나, 유아특수교사 양성기관의 학교현장실습 관련 연구는 아직 미흡한 실정이다. 이에 본 연구에서는 유아특수교육 학교현장실습과 관련된 국내 연구를 분석함으로써 동향을 탐색해보고자 하였다. 연도에 제한을 두지 않고 탐색한 결과 총 18편의 연구가 선정되었고, 게재학술지 및 발표연도, 연구대상, 연구방법, 실습기관 환경, 연구주제 및 세부내용에 따라 분석하였다. 분석 결과, 연도별로 연구들이 고르게 분포되어 있었으며, 대체적으로 대학교 4학년에 재학 중인 예비유아특수교사를 포함한 연구가 가장 많았다. 연구방법에 있어서는 질적연구와 양적연구가 비슷한 비율로 나타났다. 실습환경에 있어서는 유치원 특수학급이 가장 많았고 다음으로 특수학교로 나타났다. 연구내용에 있어서는 대체적으로 예비유아특수교사의 현황 및 실태, 인식, 경험 등을 살펴본 연구가 많았으며 그 외 실습 개선 프로그램 또는 실습생에게 피드백을 제공하는 중재 프로그램 연구는 미비한 것으로 나타났다. 이상의 연구 결과를 토대로 예비유아특수교사 학교현장실습후속 연구에 대한 제언을 제시하였다. In the education of pre-service teachers at teacher preparation programs, field-experience is a process that provides direct opportunities to develop competencies and expertises of pre-service teachers. Studies have been conducted to strengthen school field-experiences, however, research related to school field-experiences in Korean early childhood special education programs is still insufficient. Therefore, the current study aimed to explore trends by analyzing Korean studies related to early childhood special education field-experiences. A total of 18 studies were selected as a result of the search without limiting the year and analyzed according to the year of publication, subject, research method, practice institution environment, and research topic. As a result of the analysis, studies were evenly distributed by year, and most studies involved senior pre-school special education teachers in universities. In terms of research method, qualitative and quantitative research appeared at a similar rate, but no experimental research was conducted. In terms of practice environment, kindergarten special classes were the most common, followed by early childhood special schools. Regarding research topics, most studies examined the current status, perceptions, and experiences of pre-service special teachers. However, studies that examined other practicum improvement programs or perceptions of related stakeholders were found to be lacking. Based on the above research results, implications for future studies on field-experience for pre-school special education teachers were suggested.

      • KCI등재

        공공건축의 공간 공유가치를 통한 주민복지 향상에 관한 연구

        황순재 ( Soonjae Hwang ),이은영 ( Eunyoung Lee ),이돈일 ( Donil Lee ) 한국공간디자인학회 2018 한국공간디자인학회논문집 Vol.13 No.3

        (연구배경 및 목적) 시대에 따라 가치소비문화가 트렌드로 자리 잡으면서 공유경제는 미래를 이끌어 갈 메가트렌드로 부상하였다. 이러한 흐름 속에 나타난 공간 공유는 기존의 유휴공간의 문제점을 극복하고자 나타난 새로운 공간 활용법이자 경제적 접근 방법이다. 사용하지 않는 공간 공유를 통해 수요자와 이용자 서로의 이익을 이끌어 냈으며 노후화된 환경, 부족한 시설 등을 다양한 방법으로 해결하고 있다. 도심 내 새로운 시설을 만드는 것은 많은 제약이 따르며, 지속가능성과 재생에 대한 사회적 니즈와도 부합하지 않는다. 이에 공공건축의 공간 공유가치 유형들을 알아보고 그 특성을 도출하여 평가해 향후 유휴공간의 방향을 제시해보고자 한다. (연구방법) 연구방법으로는 공공건축과 공간 공유 관련 논문 및 문헌을 바탕으로 선행연구를 분석하여 공공건축과 공간 공유의 요소를 알아보고 이를 재정립하여 공공건축의 공간 공유가치 유형과 특성을 도출한다. 행정공간계와 교육공간계 별로 각각 2곳, 총 4곳의 유휴공간 활용법을 분석하고, 공간에 나타나는 특성들을 평가하여 공간 공유가치 요소들의 순위와 그 중요도를 알아보고자 한다. (결과) 가치유형으로 환경, 사회, 경제, 문화가치로 총 4가지가 도출되었으며, 세부 특성으로는 지속가능성, 이용성, 개방성, 공공성, 접근성, 경제성, 다양성, 지역성의 8가지가 도출되었다. 대상지의 사례분석 결과 공간 공유가치유형 중에서 환경가치가 가장 높게 나왔으며, 사회가치, 경제가치, 문화가치 순으로 측정되었다. 공간 공유가치 특성에서는 지속가능성과 이용성이 가장 높게 나왔으며, 개방성, 공공성, 접근성, 경제성이 뒤를 이었다. 그리고 전체적으로 지역성과 다양성의 점수가 가장 낮게 평가되었다. (결론) 합리적 가치 구매 중심의 소비문화 확산으로 현대 사회에 대한 공공의 역할은 더욱 대두되고 있으며, 다양한 기능과 목적을 수행하는 공공건축 활용에 대한 사회의 요구 또한 증가했다. 공공건축물은 행정의 본래 기능에서 나아가 사회가 요구하는 다양한 공공의 가치를 실현해야 하며 서비스를 통한 지역주민의 복지 향상의 역할을 담당하고 있다. 본 연구는 공공건축의 공간 공유가치를 유형화 및 정의하며 그에 따른 특성 세부요소를 도출하여 공간 공유가치를 발굴하고 평가하며 공간의 잠재력과 가능성에 대한 평가 지침 및 공공건축 공간의 총체적 방향성 제시에 목적을 둔다. 이에 연구를 통하여 도출된 공공건축의 공간 공유가치와 특성을 기초하여 향후 공공건축의 활용 방안과 잠재력을 재평가하며 기존 공공건축물의 활용성을 제고할 필요가 있다. (Background and Purpose) As the culture of value consumption has become a trend, the shared economy has emerged as a megatrend to lead the future. Spatial sharing in this trend is a new way of using space and an economic approach that has emerged to overcome the problems of traditional idle space. Sharing unused space has brought benefits to consumers and users, and questions of aging environment and inadequate facilities are being resolved in various ways. Creating new facilities in the city center is subject to many restrictions and does not meet social needs for sustainability and regeneration. Therefore, we will explore the types of space sharing values in public buildings, derive and evaluate their characteristics, and present the direction of unused space in the future. (Method) As a research method, a prior study was analyzed based on papers and literature related to public architecture and space sharing to discover and redefine the elements of public architecture and space sharing to derive the types and the characteristics of spatial sharing in public buildings. We will analyze two places for idle space utilization, four places for each administrative and educational service system, and we will evaluate the characteristics of space to rank and determine the importance of shared value factors. (Results) We derived a total of four types of values: environmental, social, economic and cultural values; with eight detailed characteristics: sustainability, usability, openness, publicity, accessibility, economics, diversity and geography. The site case analysis showed the highest environmental value among space sharing value types, measured in order of social value, economic value and cultural value. In terms of the value characteristics of shared space, sustainability and usability were the highest, followed by openness, publicity, accessibility and economic feasibility. And, overall, the score for regionalism and diversity was the lowest. (Conclusions) The role of the public in modern society is becoming more important with the spread of consumption culture centered on rational value purchasing, and society's demand for the use of public buildings to fulfill various functions and objectives has also increased. Public buildings must realize the various public values demanded by society, in addition to their original function of administration, and are responsible for improving the welfare of local residents with their services. This study is designed to model and define the value of spatial sharing in public buildings, to derive detailed characteristics, to explore and evaluate the value of spatial sharing, and to assess the potential and potential value of space to the public. Therefore, it is necessary to reevaluate future utilization methods, the potential of public buildings based on space sharing values and the characteristics of public buildings derived through research in order to enhance the usability of existing public buildings.

      • KCI등재

        인터넷을 통한 도움 행위에서 이타 성격과 성 역할에 대한 탐색적 연구

        이규동(Gyudong Lee),이재은(Jaeeun Lee),권순재(Soonjae Kwon) 한국인터넷전자상거래학회 2010 인터넷전자상거래연구 Vol.10 No.4

        In general, people tried to help others regardless of their personal benefits. This self-sacrificing behavior is observed not only in the offline world, but also on the Internet. Because the Internet has become such an important part of everyday life in many countries, helping behavior on the Internet is a routine occurrence. However, research on the positive side of the Internet such as helping is insufficient. Although many types of helping exist, we focus on helping behaviors targeted at strangers. Because helping stranger is a self-sacrificing behavior, research on this domain provides useful insights into individuals’ psychological aspects. More specifically, this paper investigates the effects of altruistic traits, empathy, distress, and the gender of potential helper on helping stranger. This study has employed two stages of experimental approach based on scenarios to explain helping behavior and compare it in online vs. offline surroundings. The results reveal the followings: 1) altruistic traits and empathy are correlated positively with helping male targets online and 2) distress and bystanders’ gender are related to helping female targets offline. This paper explains the reasons that people help strangers in online and off-line contexts, from the perspectives of social and evolutionary psychologies. Theoretical contributions and practical implications are discussed.

      • Comparison of Glycosylation Patterns and Activities of Native and CHO cell-expressed Human alpha1-Antitrypsins

        Kyung Jin Lee,Sang Mee Lee,Jin Young Gil,Ohsuk Kwon,Jin Young Kim,Soonjae Park,Hye-Shin Chung,Doo-Byoung Oh 한국당과학회 2012 한국당과학회 학술대회 Vol.2012 No.1

        Human alpha-1-antitrypsin (α1AT) is a glycoprotein with protease inhibitor activity protecting tissues from degradation. Patients with inherited α1AT deficiency are treated with native α1AT (nAT) purified from human plasma. In the present study, recombinant α1AT (rAT) was produced in Chinese hamster ovary (CHO) cells and their glycosylation patterns, inhibitory activity and in vivo half-life were compared with those of nAT. A peptide mapping analysis employing a deglycosylation reaction confirmed full occupancy of all three glycosylation sites and the equivalency of rAT and nAT in terms of the protein level. N-glycan profiles revealed that rAT contained 10 glycan structures ranging from bi-antennary to tetra-antennary complex-type glycans while nAT displayed six peaks comprising majorly bi-antennary glycans and a small portion of tri-antennary glycans. In addition, most of the rAT glycans were shown to have only core α(1,6)-fucose without terminal fucosylation, whereas only minor portions of the nAT glycans contained core and Lewis X-type fucose. As expected, all sialylated glycans of rAT were found to have α(2,3)-linked sialic acids, which was in sharp contrast to those of nAT, which had mostly α(2,6)-linked sialic acids. However, the degree of sialylation of rAT was comparable to that of nAT, which was also supported by an isoelectric focusing gel analysis. Despite the differences in the glycosylation patterns, both α1ATs showed nearly equivalent inhibitory activity in enzyme assays and serum half-lives in a pharmacokinetic experiment. These results suggest that rAT produced in CHO cells would be a good alternative to nAT derived from human plasma.

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