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      • KCI등재

        Report on 30 unrecorded bacterial species of the phylum Firmicutes isolated from Korea in 2016

        Nahar, Shamsun,Lee, Do-Hoon,Bae, Jin-Woo,Im, Wan-Taek,Jahng, Kwang Yeop,Joh, Kiseong,Kim, Wonyong,Lee, Soon Dong,Yi, Hana,Cha, Chang-Jun The National Institute of Biological Resources 2018 Journal of species research Vol.7 No.1

        During the course of investigation of indigenous prokaryotic species in Korea, a total of 30 bacterial strains belonging to the phylum Firmicutes were isolated from diverse environmental sites such as soil, avian feces, wastewater treatment plants, fermented vegetables, seawater, algae, sea cucumber, octopus and tidal flat sediment. Phylogenetic analysis based on 16S rRNA gene sequences revealed that each strain showed high sequence similarity (${\geq}98.7%$) to the closest type strain and formed a robust phylogenetic clade with the most closely related species in the phylum Firmicutes. To date, there is no official record of these 30 species in Korea. Therefore, we report 26 species of 12 genera in the order Bacillales and 4 species of 4 genera in the order Lactobacillales which have not been reported in Korea. Morphological and biochemical characteristics, isolation sources and NIBR deposit numbers are described in the species descriptions.

      • KCI등재후보

        Report on 30 unrecorded bacterial species of the phylum Firmicutes isolated from Korea in 2016

        Shamsun Nahar,차창준,배진우,임완택,장광엽,조기성,김원용,이순동,이하나,이도훈 국립생물자원관 2018 Journal of species research Vol.7 No.1

        During the course of investigation of indigenous prokaryotic species in Korea, a total of 30 bacterial strains belonging to the phylum Firmicutes were isolated from diverse environmental sites such as soil, avian feces, wastewater treatment plants, fermented vegetables, seawater, algae, sea cucumber, octopus and tidal flat sediment. Phylogenetic analysis based on 16S rRNA gene sequences revealed that each strain showed high sequence similarity (≥98.7%) to the closest type strain and formed a robust phylogenetic clade with the most closely related species in the phylum Firmicutes. To date, there is no official record of these 30 species in Korea. Therefore, we report 26 species of 12 genera in the order Bacillales and 4 species of 4 genera in the order Lactobacillales which have not been reported in Korea. Morphological and biochemical characteristics, isolation sources and NIBR deposit numbers are described in the species descriptions.

      • KCI등재

        Lichen-Associated Bacterium, a Novel Bioresource of Polyhydroxyalkanoate (PHA) Production and Simultaneous Degradation of Naphthalene and Anthracene

        ( Shamsun Nahar ),( Min-hye Jeong ),( Jae-seoun Hur ) 한국미생물 · 생명공학회 2019 Journal of microbiology and biotechnology Vol.29 No.1

        Lichens are generally known as self-sufficient, symbiotic life-forms between fungi and algae/cyanobacteria, and they also provide shelter for a wide range of beneficial bacteria. Currently, bacterial-derived biodegradable polyhydroxyalkanoate (PHA) is grabbing the attention of many researchers as a promising alternative to non-degradable plastics. This study was conducted to develop a new method of PHA production using unexplored lichen-associated bacteria, which can simultaneously degrade two ubiquitous industrial toxins, anthracene and naphthalene. Here, 49 lichen-associated bacteria were isolated and tested for PHA synthesis. During the GC-MS analysis, a potential strain of EL19 was found to be a 3-hydroxyhexanoate (3-HHx) accumulator and identified as Pseudomonas sp. based on the 16S rRNA sequencing. GC analysis revealed that EL19 was capable of accumulating 30.62% and 19.63% of 3-HHx from naphthalene and anthracene, respectively, resulting in significant degradation of 98% and 96% of naphthalene and anthracene, respectively, within seven days. Moreover, the highly expressed phaC gene verified the genetic basis of PHA<sub>mcl</sub> production under nitrogen starvation conditions. Thus, this study strongly supports the hypothesis that lichen-associated bacteria can detoxify naphthalene and anthracene, store energy for extreme conditions, and probably help the associated lichen to live in extreme conditions. So far, this is the first investigation of lichen-associated bacteria that might utilize harmful toxins as feasible supplements and convert anthracene and naphthalene into eco-friendly 3-HHx. Implementation of the developed method would reduce the production cost of PHA<sub>mcl</sub> while removing harmful waste products from the environment.

      • KCI등재

        Oscillatory shearing behavior of rocket leaves powder incorporated dough

        Abdulwahab Salem Almusallam,Jasim Ahmed,Shamsun Nahar,Siby Chacko 한국유변학회 2016 Korea-Australia rheology journal Vol.28 No.2

        Dough blended with rocket leaves powder was subjected to small and large amplitude oscillatory shears. Small amplitude oscillatory shear data were fitted to a discrete relaxation model of elastic solids and to a critical gel model. The small amplitude relaxation spectrum was thereafter used to calculate the LAOS predictions of various large deformation models. The LAOS theoretical calculations using the Phan-Thien model showed good agreement with the first harmonic stress data, and only qualitative agreement with the third and the fifth harmonic stress values. Lissajous curves showed dissimilarity in shape between the experimental data and Phan-Thien model. The network model of Sim et al. (2003). Did not have the butterfly shape displayed in the Phan-Thien model, but it provided a worse fit to stress harmonics than the Phan- Thien model. An improved damage function was proposed, where time effect on network damage was taken into consideration, and fits to stress harmonics and to Lissajous stress-strain curves were significantly improved.

      • Role of the HPV DNA Test in Follow-up of Treated Cervical Intraepithelial Neoplasia in Bangladesh

        Nessa, Ashrafun,Rashid, Mohammad Harun Ur,Jahan, Munira,Ferdous, Noor-E,Nahar, Pervin Akhter Shamsun,Chowdhury, Afroza Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.19

        Background: Cervical cancer is a major public health problem in Bangladesh. Persistence of high risk human papillomavirus (HRHPV) influences the progression of the disease, with an important role in followup for cervical intraepithelial neoplasia (CIN). Objective: To establish application of high risk HPV DNA test in the follow-up of women after treatment of CIN. Materials and Methods: This cross-sectional and hospital based study was carried out among 145 CIN treated women during the previous six months to three years at the colposcopy clinic of Bangabandhu Sheikh Mujib Medical University, Dhaka, between January 2011 and June 2012. Pap smear and HPV samples were collected and colposcopy was performed to find out the persistence of the disease. Cervical samples obtained were tested for HPV DNA using the Hybrid Capture II (HC-II) test. A cervical biopsy was collected whenever necessary. The results were compared to assess the efficacy of different methods during follow up such as Pap smear, HPV test and colposcopy. Results: Mean age of the recruited women (n=145) was 33.6 (${\pm}7.6$), mean age of marriage was 16.8 (${\pm}2.9$) and mean age of 1st delivery was 18.8 (${\pm}3.5$) years. More than half had high grade CIN before treatment and 115 (79.3%) women were managed by LEEP and 20.7% were managed by cold coagulation. Among the 145 treated women, 139 were negative for HPV DNA and six of them (4.1%) were HPV positive. Sensitivity of Pap smear (40.0) and HPV DNA test (40.0) was poor, but specificity was quite satisfactory (>93.0) for all the tests. Conclusions: The high risk HPV DNA test can be an effective method of identifying residual disease. It can be added to colposcopy and this should be applied to all treated women attending for their first or second post-treatment follow-up visit at 6 months to one year, irrespective of the grade of treated CIN.

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