간내 담석과 동반된 간내 담도암에서의 p53과 Ki-ras의 발현

김명환,이성구,서동완,이승규,남승우,박능화,민영일,김연석,심기남,공경엽 대한소화기학회 1997 대한소화기학회지 Vol.30 No.5

Background/Aims: Hepatolithiasis or primary intrahepatic stones may be accompanied later by intrahepatic cholangiocarcinoma. This cancerous lesion is frequently associated with atypical hyperplastic epithelium that was suspected of being precancerous. To investigate the Ki-ras or p53 mutation may play a role in carcinogenesis, and to determine whether atypical hyperplastic epithelium may be precancerous, this study was performed in intrahepatic cholangiocarcinomas associated with hepatolithiasis. Methods: We examined 12 patients with intrahepatic cholangiocarcinomas associated with hepatolithiasis. Ki-ras point mutations were assessed by direct DNA sequencing. Expressions of p53 protein were immunohistochemically assessed. The overexpression of p53 and point mutation of Ki-ras were examined in normal, hyperplastic, atypical hyperplastic, and can- cerous tissues separately. Results: The expressions of p53 protein were detected in 4 (33%) of 12 cholangiocarcinomas and Ki-ras point mutations at codon 12 were found in 2 (17%) of 12 cholangiocarcinomas. In those two carcinoma cases which contained the mutant sequence of Ki-ras, the same point mutation from wild type GGT (glycine) to GAT (aspartic acid) was. found in the associated atypical hyperplastic epithelium. However, none of the normal and hyperplastic epithelium harbored Ki-ras or p53 mutations. Conclusions: The overexpression of p53 may play a part in the carcinogenesis of some intrahepatic cholangiocarcinomas associated with hepatolithiasis, whereas the role of Ki-ras mutations in those cases is dubious. However, Ki-ras point mutation at codon 12 may be responsible for either cancer or atypical hyperplastic epithelium associated with hepatolithiasis in certain cases, suggesting atypical hyperplastic epithelium may give rise to carcinoma.

연약지반중의 흙막이구조물의 거동해석에 관한 연구

안종필,기완서,오영신 조선대학교 국토개발연구소 1997 국토개발연구 Vol.17 No.1

Recently, a rapid economical development and industrialization require large amount of land of industrial estate and expansion of citis. Therefore the construction of large and deep basements takes place in built up areas where the braced excavation for earth retaining structures causes many problems such as settlement and damages of nearby buildings and underground utilities. This study deals with the behavior of the retaining earth structures and the ground movement around it based on the measuring results which were obtained from the two ground sites. The results of elasto-plastic wall design program involving the effect of lateral flow pressure showed a good agreement with the output of finite difference computer program which covers the behavior of wall as the ground movement around it.

강우시 불포화 성토사면의 안정해석

심태섭,기완서,김선학,최순범 朝鮮大學校 建設技術硏究所 2005 建設技術硏究 Vol.25 No.1

This study conducted the indoor slope model test which reproduces the rainfall after building up the artificial slope by considering the construction condition of the actual slope in order to understand the characteristics of the change of the quantitative value such as the pore water pressure, dangerous rainfall volume etc, in accordance with the superior seepage of the unsaturated banking slope in the time of rainfall. With this result. the change of the pore water pressure caused by the rainfall seepage, the estimation of time for the slope degradation and of the dangerous rainfall volume, the change of the seepage line, the characteristics of the change in safety rate by using the simplified Janbu's method (1968) have been analyzed and studied. The abstract for this study is as follows: 1. Since the soil within the slope is in the unsaturated state in the early period after the rainfall started, it is started at the pore water pressure of the negative value(-) in the measuring point around the outer layer and of the positive value(+) in the deep area. As the rainfall permeates into the slope, it become saturated, therefore, the pore water pressure also increases which lead to the degradation. After that, it tends to be gradually diminished. The pore water pressure had shown relatively considerable increase right before the degradation, and 50~150 minutes before the slope degradation happens, it had shown sharp increase. 2. The safety rate is sharply decreased when reached the slope layer after being started its activity at the slope line area; the time to the complete degradation of the slope layer by being the 1.0 for the safety rate after the degradation at the slope line area started is displayed short as the rainfall intensity strengthens, and longer as the slope inclination becomes gentler. Likewise, the time to the complete degradation from the slope line to the slope layer is relatively short for the slope built by the 8M than by the one built by the SW.

PBT에 의한 얕은기초의 지지력 및 침하량 산정연구

심태섭,기완서,주승완,김선학 朝鮮大學校 建設技術硏究所 2002 建設技術硏究 Vol.22 No.1

A stable and economical bearing force estmate and the upper load condition and foundation ground characteristics are very important in a case of designing shallow foundation. A typicial field test method which get the coefficient of subgrade reaction to estmate bearing capacity of the earthand sand ground foundation and settlement of the soil and rock foundation with most direct method of the field test methods to estimate bearing capacity, is plate bearing test(PBT). So this study refer's to the calculation characteristics of the bearing capacity and settlement according to plate bearing method of the A field and B field which is practiced in the soil of weathered gneiss and soil reclamation ground, and refers to the reasonable settlement calculation method and bearing capacity estmate and safety examination method suitable to the soil of wethered rock and soil reclamation ground according to the plate bearing test result when we examine the structure safety of same design term by awllowable bearing capacity calculation in the point of view of bearing capacity and settlement. Therefore in the methods to determine yield bearing capacity that is applied to allowable bearing capacity calculation when we design shallow foundation and examine safety by plate bearing test, P-S analysis, logP-logS analysis and logP-S analysis are proper methods and it is proper to examine safety by the point of bearing capacity in the soilground to be able t o find the yield point in the Load-Settlement behavior curve.

강우시 침투영향을 고려한 사면안정해석

심태섭,기완서,김선학,최순범 朝鮮大學校 建設技術硏究所 2007 建設技術硏究 Vol.27 No.2

Most of current slope safety analyses consider only the effect of the rise of underground water level. However, because the infiltration of surface water into the ground is believed to have significant effects on slope stability, the present study performed slope safety analysis in case of the rise of underground water level, the infiltration of surface water, and both resulting from rainfall, and examined their effects. For this study, we selected Slope A and B, at which slope failure had happened due to rainfall, and made slope safety analysis in consideration of the fall of wetting front and the rise of underground water level resulting from rainfall. Based on the results of the analysis, we made comparative analysis on the relation between the effect of infiltration on rainfall and the stability of the slopes and proposed a desirable slope safety analysis method. The conclusions of this study are as follows. 1. In slope safety analysis in consideration of the effect of infiltration resulting from rainfall, the variation of safety factor was different depending on whether to consider the fall of wetting front, the rise of underground water level. or both. 2. The decrease of the slope safety factor caused by the fall of wetting front on rainfall was somewhat faster than that caused by the rise of underground water level. This suggests that slope failure caused by rainfall is affected more by the fall of wetting front than by the rise of underground water level. 3. When both the fall of wetting front and the rise of underground water level were considered at the same time, the slope safety factor decreased faster than when either the fall of wetting front or the rise of underground water level was considered. Accordingly, it is considered desirable to consider both the fall of wetting front and the rise of underground water level in slope safety analysis that takes into account the effect of infiltration resulting from rainfall.

Geotextile 利用效果에 관한 硏究

鄭永同,奇完敍 조선대학교 국토개발연구소 1989 국토개발연구 Vol.9 No.1

In construction of embankment over soft strata in road and river, Geotextile has been increasingly used recently after identified that it was very economical and effective as seperater, reinforceing material, and filter of soft ground. This study deals with the effects of bearing capacity, permeability and stability by computing using STABR program of Modified Simplified Bishop method. The STABR program is applied to Hyunsan River and Hakkyo River embankments for the stability analysis in cases of with and without Geotextile. The conclusions are as follows; 1) The increase of safety of river embankment is not so much in case of with Geotextile. 2) From the results of permeability varying procedure analysis for the embankment construction of Sapkyo dyke. it reveals about 100 times as decreased as initial permeability coefficient in one year.

지반굴착시 J.S.P 공법에 의한 지반보강 효과에 관한 연구

심태섭,기완서,주승완 조선대학교 국토개발연구소 1997 국토개발연구 Vol.17 No.1

Recently, in order to utilize of underground space, a large scales underground excavation work including large building basements and subway construction have a tendency to increase rapidly in urban areas. This study deals with the horizontal displacement, Bending Moment, Shearing Force of the Propped retaining walls, based on the measuring results which were obtained from two excavation construction stations, and for the purpose of reinforcement of ground and cut-off of ground water behind the Propped retaining wall, high pressure jet grouting is widely used. Therefor the purpose of this paper is to investigate the effects of jet grouting on ground reinforcement and cut-off the ground water behind Propped retaining walls. The results of permeability improved ground were 1×10^-3cm/sec smaller than those of the original ground and the high pressure jet grouting hassufficient effects on reinforcement of ground.

다짐도에 따른 화강풍화토의 역학적 특성

심태섭,기완서,주승완,김선학 朝鮮大學校 建設技術硏究所 2002 建設技術硏究 Vol.22 No.1

In this study do we collect the Wethered Granite Soil of Gwang-ju Pung -am district and Youm-ju-dong Gwang-ju world cup stadium construction field and identify physical character according to basic physical test and summerged the sample of 85% degree of compaction in the dry side and wet side in a water tank for ten days on the basis of the sample of 80%, 85%, 90% degree of compaction and Optimum Moisture Ratio in the Optimum Moisture Ratio state in order to exam permeability and compression character of the Weathered Granite Soil according to the compaction state of retaining wall, abutment bankfill banking. We perform falling head permeability test and them standard compaction test and calculate coefficient of permeability and consolidation constant according to the test result. We have a mind to clear permeability and compression character of the Weathered Granite Soil of this study area according to relation curve of the calculated coefficient of permeability, consolidation constant and void ratio, degree of compaction. Therefore compression constant, according to the compaction energy of Optimum Moisture Ratio, is in inverse proportion linearally in the Weathered Granite Soil of WGS-1 and WGS-2 and coefficient of permeability increase directly in a range of void ratio e=0.627∼0.779. e=0.6.57∼0.800.

An Endogenous Proteinacious Inhibitor for S-Adenosyl-L-methionine-dependent Transmethylation Reactions; Identification of S-Adenosylhomocysteine as an Integral Part

Seo, Dong-Wan,Han, Jeung-Whan,Hong, Sung-Youl,Paik , Woon-Ki,Lee, Hyang-Woo The Pharmaceutical Society of Korea 1999 Archives of Pharmacal Research Vol.22 No.3

A proteinacious inhibitor with a molecular weight of 1,600 Da which inhibits S-adenosyl-L-methionine-dependent transmethylation reactions was purified from porcine liver to homogeneity by procedures including boiling, Sephadex G-25 column chromatography and repeated HPLC. Employing both Nuclear Magnetic Resonance (NMR) and Fast Atom Bombardment-Mass (FAB-Mass) spectroscopy, S-adenosylhomocysteine was conclusively identified as an integral part of the inhibitor. The purified S-adenosylhomocysteine was competitive with S-adenosyl-L-methionine with Ki value of $6.3{\times}10^{-6}$ M towards protein methylase II.

An Endogenous Proteinacious Inhibitor for S-Adenosyl-L-methionine-dependent Transmethylation Reactions : Identification of S-Adenosylhomocysteine as an Integral Part

Seo, Dong Wan,Han, Jeung Whan,Hong, Sung Youl,Paik, Woon Ki,Lee, Hyang Woo 성균관대학교 약학연구소 1999 成均藥硏論文集 Vol.11 No.-

A proteinacious inhibitor with a molecular weight of 1,600 Da which inhibits S-adenosyl-L-methionine-dependent transmethylation reactions was purified from porcine liver to homogeneity by procedures including boiling, Sephadex G-25 column chromatography and repeated HPLC. Employing both Nuclear Magnetic Resonance (NMR) and Fast Atom Bombardment-Mass (FAB-Mass) spectroscopy, S-adenosylhomocysteine was conclusively identified as an integral part of the inhibitor. The purified S-adenosylhomocysteine was competitive with S-adenosyl-L-methionine with Ki value of 6.3 +10^-6 M towards protein methylase II.