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Rongjun Chen,Yongrong Liao,Shengbin Liu,Yunyun Jiang,Changqiong Hu,Xuewei Zhang,Xufeng Cao,Zhengjun Xu,Xiaoling Gao,Lihua Li,Jianqing Zhu 한국유전학회 2017 Genes & Genomics Vol.39 No.1
Dirigent (DIR) and DIR-like family genes were involved in lignification or in the response to pathogen infection and abiotic stress in plants. Little is known to us about how rice DIR genes respond to adverse conditions. In this study, we reported genome-wide analysis of 49 DIR or DIR-likes genes in rice. The 49 OsDIRs or OsDIR-likes were tandem arranged into ten clusters. The phylogenetic analysis indicated that the 49 rice DIR and DIR-like genes cluster into five distinct subfamilies, DIR-a and four DIRlike subfamilies (DIR-b/d, and DIR-g, DIR-c, DIR-e). Meta-analysis of microarray gene expression datas indicated that all the OsDIRs or OsDIR-likes were expressed almost at the same level but with different patterns: most OsDIRs or OsDIR-likes were expressed exclusively in stigma and ovary and were induced by IAA and BAP; several genes were induced by trans-zeatin (tZ) and DMSO; 23 OsDIRs or OsDIR-likes were responded to abiotic stress. Our analysis also showed that most of these genes could respond to abiotic stresses, which contained cis-regulatory elements. The present study will provide a useful reference for further functional analysis of the DIR genes in rice.
Rongjun Chen,Xun Wu,Mengliang Tian,Yubi Huang 한국유전학회 2011 Genes & Genomics Vol.33 No.5
The global gene expression changes in response to low nitrogen stress in leaf tissues of two maize inbred lines(Mo17 and Hz4) with contrasting low nitrogen tolerance was analyzed using the Affymetrix maize genome array. Our results showed 887 genes involved in a variety of biological processes were responsive to low nitrogen treatment in Mo17, out of which 384 were up-regulated and 503 were down-regulated. In Hz4the expression of 1108 genes was changed, of which 696 were up-regulated and 412 genes were down-regulated. The gene expression profiles also revealed 1799 genes differentially expressed (910 and 889 genes with higher level of expression in Hz4 and Mo17, respectively) between the two lines under low nitrogen conditions. These results explain at the transcript level why Mo17 is more sensitive than Hz4 under low nitrogen stress. In addition to previously reported nitrogen response genes (nitrate transporter, nitrite reductase, nitrate reductase,ferredoxin) we found many new nitrogen response genes (early light-inducible protein,uroporphyrinogen methyltransferas e,phosphoenolpyruvate carboxylase, tonoplast intrinsic protein,sesquiterpene cyclase). Our results not only provide new insights on the molecular mechanisms of nitrogen stress, but also serve as a valuable resource to researchers who aim to improve the efficiency of maize nitrogen use.
Genome-wide analysis and environmental response profiling of SOT family genes in rice (Oryza sativa)
Rongjun Chen,Yunyun Jiang,Jiali Dong,Xin Zhang,Hongbo Xiao,Zhengjun Xu,Xiaoling Gao 한국유전학회 2012 Genes & Genomics Vol.34 No.5
Sulphotransferase (SOT) catalyses the transfer of a sulphonate group from 3'-phosphoadenosine 5'-phosphosulphate (PAPS)to an appropriate hydroxyl group of various substrates with the parallel formation of 3'-phosphoadenosine 5'-phosphate (PAP). Although several SOTs have been identified and characterized in mammalian, their role in plant is still unclear. In this study, we report genome-wide comprehensive expression analysis of 35 putative SOT genes in rice. The 35 OsSOTs were tandemly arranged into six clusters. The phylogenetic analysis showed that there were 7 subfamilies of OsSOTs and 11 putatively conserved motifs. Six OsSOTs might be pseudogenes,25 have the two motifs which were involved in PAPS binding regions I and IV. Microarray data indicated that all the OsSOTs were expressed almost at the same level but with different patterns: most OsSOTs were expressed exclusively in stigma and ovary and induced by IAA and BAP, several genes were induced by tZ and DMSO and 11 OsSOTs were response to abiotic stress. Further analysis showed that these 11 genes contained cis-regulatory elements responding to abiotic stresses.
Compressed Sensing-based Multiple-target Tracking Algorithm for Ad Hoc Camera Sensor Networks
( Xu Lu ),( Lianglun Cheng ),( Jun Liu ),( Rongjun Chen ) 한국인터넷정보학회 2018 KSII Transactions on Internet and Information Syst Vol.12 No.3
Target-tracking algorithm based on ad hoc camera sensor networks (ACSNs) utilizes the distributed observation capability of nodes to achieve accurate target tracking. A compressed sensing-based multiple-target tracking algorithm (CSMTTA) for ACSNs is proposed in this work based on the study of camera node observation projection model and compressed sensing model. The proposed algorithm includes reconfiguration of observed signals and evaluation of target locations. It reconfigures observed signals by solving the convex optimization of L1-norm least and forecasts node group to evaluate a target location by the motion features of the target. Simulation results show that CSMTTA can recover the subtracted observation information accurately under the condition of sparse sampling to a high target-tracking accuracy and accomplish the distributed tracking task of multiple mobile targets.
Fujun Cao,Ping Yin,Jiang Zhang,Hou Chen,Rongjun Qu 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.4
Nanoplates of cobalt phosphonate poly-Co3(LH2)2(LH3)2(H2O)4 2H2O (denoted as poly-CoAedp, L = 1- aminoethylidenediphosphononic acid anion) with two-dimensional structure has been successfully synthesized under hydrothermal conditions and applied in the removal of Pb(II) and Hg(II) ions from aqueous solutions. Poly-CoAedp has exhibited a good performance for heavy metal removal, especially for Pb(II). The effects of different experimental parameters on the competitive adsorption capacities were investigated. The research results suggest that the nanoplates of poly-CoAedp have great potential applications in environmental protection, and the good adsorption ability make it a good promising candidate material for lead removal.
( Yaodong Liu ),( Sibtain Ahmed ),( Yaowei Fang ),( Meng Chen ),( Jia An ),( Guang Yang ),( Xiaoyue Hou ),( Jing Lu ),( Qinwen Ye ),( Rongjun Zhu ),( Qitong Liu ),( Shu Liu ) 한국미생물생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.4
Chitin deacetylase (CDA) inhibitors were developed as novel antifungal agents because CDA participates in critical fungal physiological and metabolic processes and increases virulence in soil-borne fungal pathogens. However, few CDA inhibitors have been reported. In this study, 150 candidate CDA inhibitors were selected from the commercial Chemdiv compound library through structure-based virtual screening. The top-ranked 25 compounds were further evaluated for biological activity. The compound J075-4187 had an IC50 of 4.24 ± 0.16 μM for AnCDA. Molecular docking calculations predicted that compound J075-4187 binds to the amino acid residues, including active sites (H101, D48). Furthermore, compound J075-4187 inhibited food spoilage fungi and plant pathogenic fungi, with minimum inhibitory concentration (MIC) at 260 μg/ml and minimum fungicidal concentration (MFC) at 520 μg/ml. Therefore, compound J075-4187 is a good candidate for use in developing antifungal agents for fungi control.