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RISS 인기검색어
- 검색키워드
- 저자 : Mengliang Tian (검색결과 2 건)
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Rongjun Chen,Xun Wu,Mengliang Tian,Yubi Huang 한국유전학회 2011 Genes & Genomics Vol.33 No.5
The global gene expression changes in response to low nitrogen stress in leaf tissues of two maize inbred lines(Mo17 and Hz4) with contrasting low nitrogen tolerance was analyzed using the Affymetrix maize genome array. Our results showed 887 genes involved in a variety of biological processes were responsive to low nitrogen treatment in Mo17, out of which 384 were up-regulated and 503 were down-regulated. In Hz4the expression of 1108 genes was changed, of which 696 were up-regulated and 412 genes were down-regulated. The gene expression profiles also revealed 1799 genes differentially expressed (910 and 889 genes with higher level of expression in Hz4 and Mo17, respectively) between the two lines under low nitrogen conditions. These results explain at the transcript level why Mo17 is more sensitive than Hz4 under low nitrogen stress. In addition to previously reported nitrogen response genes (nitrate transporter, nitrite reductase, nitrate reductase,ferredoxin) we found many new nitrogen response genes (early light-inducible protein,uroporphyrinogen methyltransferas e,phosphoenolpyruvate carboxylase, tonoplast intrinsic protein,sesquiterpene cyclase). Our results not only provide new insights on the molecular mechanisms of nitrogen stress, but also serve as a valuable resource to researchers who aim to improve the efficiency of maize nitrogen use.
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Shipeng Yang,Qiwen Zhong,Jie Tian,Lihui Wang,Mengliang Zhao,Li Li,Xuemei Sun 한국유전학회 2018 Genes & Genomics Vol.40 No.10
In recent years, Jerusalem artichoke has received widespread attention as a novel source of sugar, biofuel, and animal feed. Currently, only few gDNA-SSRs derived from sunflower were verified in the Jerusalem artichoke; therefore, it is particularly important to develop SSR primer markers that belonged to Jerusalem artichoke resources. Using EST data to develop EST-SSR markers is simple and effective. In order to understand the general characteristics of SSR markers in Jerusalem artichoke EST sequences and accelerate the use of SSR markers in Jerusalem artichoke research. This study used 40,370 sequenced unigene fragments and MISA software to identify SSR loci. The 48 pairs of EST-SSR primers assessed for the identification of 45 varieties of Jerusalem artichoke. Cluster, genetic diversity parameters and AMOVA analysis was conducted using the genetic similarity coefficient, revealing genetic differences between 48 genetic material. A total of 1204 SSR loci were identified with 13 different types of repeats, distributed among 1020 EST sequences, of which trinucleotide repeats were the most common, accounting for 38.21% of the total SSR loci. Among the 44 repeat motifs, AG/CT, AAG/ CTT, and ATC/ATG motifs had the highest frequencies, accounting for 22.45, 14.71, and 7.84% of all motifs, respectively. From these sequences, 48 pairs of EST-SSR primers were designed, and 22 primer pairs for loci with high polymorphism were selected to analyze the genetic diversity of 45 Jerusalem artichoke germplasm sources. The results indicated that the variation range of the effective number of alleles for 22 primers ranged between 1.7502 and 4.5660. The Shannon’s information index ranged between 0.6200 and 1.6423. The variation range of PIC ranged between 0.3121 and 0.6662 with an average of 0.5184. Cluster analysis was conducted using the genetic similarity coefficient, revealing significant genetic differences between Asian and European genetic material. Cluster analysis revealed a relationship between the genotypes and geographic origins of the Jerusalem artichoke. The results of AMOVA as well as the genetic identity and genetic distance in the Jerusalem artichoke population showed that there presented certain genetic heterogeneity in Jerusalem artichoke genetic structure of 45 samples from seven different geographic populations. The Jerusalem artichoke EST-SSR marker system established in this study provides an effective molecular marker system for future research focused on Jerusalem artichoke genetic diversity and the breeding of new varieties.
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