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나환식 ( Hwan Sik Na ),김진영 ( Jin Young Kim ),박학재 ( Hak Jae Park ),최경철 ( Gyeong Cheol Choi ),양수인 ( Soo In Yang ),이지헌 ( Ji Heon Lee ) 한국식품저장유통학회(구 한국농산물저장유통학회) 2013 한국식품저장유통학회지 Vol.20 No.3
국내에서 주로 재배되는 무화과 품종인 도후인(Dauphine)2종, 봉래시(Horaish)2종, 바나네(Banane) 1종을 대상으로 이화학적 특성을 분석한 결과, 무화과의 수분은 85.37~87.28%, 회분 0.38~0.43%, 조지방 0.20~0.26%, 조단백질 0.39~0.81%로 품종별 차이는 보이지 않았으며 조섬유는 바나네가 7.55±0.13%로 다른 품종 5.12~7.60%에 비해 다소 높은 결과를 보였다. 무기성분 함량은 모든 품종에서 K>Ca>Mg>Na순으로 나타났으며 전체 함량의 경우 도후인 품종이 타 품종에 비해 조금 높은 결과를 보였다. 바나네 품종과 봉래시 품종의 총 유리아미노산 조성은 glutamic acid>proline>aspartic acid 순으로 높은 분포를 보였으며, 전체 함량의 51.68~58.71%를 차지하고 있으며, 도후인 품종의 경우 glutamic acid>aspartic acid>alanine이 전체의 약 50%를 차지하고 있었다. 또한 기억력 감퇴 예방 등 학습 능력에 도움을 주는 기능성 물질인 γ-aminobutyric acid(GABA) 함량은 바나네에서 26.69mg/100g으로 상당히 높은 함량을 보였다. 총 폴리페놀 함량은 도후인이 198.91~261.64mg/kg, 봉래시 품종이 169.90~174.33mg/kg, 바나네 품종이 211.07mg/kg으로 나타났으며 총 플라보노이드 함량은 바나네(84.95mg/kg), 도후인(60.47~68.71mg/kg), 봉래시(44.12~44.60mg/kg)순으로 바나네가 가장 높았으며 품종간의 차이를 보였따. 플라보노이드성분 중 quercetin과 kaempferol은 각각 2.40~3.54mg/kg, 1.09~1.16mg/kg으로 분석되었다. In this study, the proximate compositions, minerals, free amino acids, total polyphenol and flavonoids of figs were analyzed according to their cultivars. The proximate compositions showed moisture at 85.37~87.28%, crude ash at 0.38~0.43%, crude lipid at 0.20~0.26%, crude protein at 0.39~0.81%, carbohydrate at 11.23~13.66% and crude fiber at 5.12~7.55% (dry base). The amounts of the following minerals in the figs were highest, in this order: K > Ca > Mg > Na. The total polyphenol contents were highest in this order: Dauphine (198.91~261.64mg/kg), Banane (211.07 mg/kg) and Horaish (169.90~174.33 mg/kg). The total flavonoid contents were highest in this order. Banane (84.52 mg/kg) > Dauphine (60.47~68.71 mg/kg) > Horaish (44.12~44.60mg/kg). The quercetin contents were highest in this order. Dauphine, 2.40~3.54 mg/kg; Banane, 3.54 mg/kg; and Horaish, 2.40~2.75 mg/kg; but the flavonoid contents were lowest in this order. Dauphine, 1.11~1.16 mg/kg; Banane, 1.14 mg/kg; and Horaish, 1.09~1.11 mg/kg. The free amino acid content of the figs was 199.70~328.77 mg/100g; their essential free amino acid contents, 46.45~67.46 mg/100 g; and their GABA (γ-aminobutyric acid) contents, 13.57~26.69 mg/100 g.
Hwa-Young Seo,Gyeong-Ju Lee,In-Su Hwang,Mi-Sook Na,Yong-Seok Choi 한국응용곤충학회 2018 한국응용곤충학회 학술대회논문집 Vol.2018 No.10
Metcalfa pruinosa is the agricultural pest, first reported in Seoul, Gyeong-gi, Gyeongnam, Korea in 2009. This pests are difficult to control due to the wide range of hosts such as beans, apples, ginseng, pears, persimmon, and peaches. This study was carried out to investigate the ecological characteristics of M. pruinosa in pepper cultivation area. As a result of the density of M. pruinosa survey in Chungnam, Korea in 2018, the density of wintering-eggs, nymph, adult was the largest in Dang-jin area. And the results of the ecological characteristcs survey of M. pruinosa in red pepper cultivation area of Korea, M. pruinosa didn’t enter a red pepper field. In addition, the possibility of using three kinds of plants as attraction plants was examined in order to control environmentally friendly M. pruinosa. Among them, the greatest number of M. pruinosa nymph were attracted to Sunflower and Green perilla during nymph stage.
( Gyeong Been Lee ),( Jienny Lee ),( Yong Woo Sohn ),( Na Yeon Gu ),( Hee Ryang Kim ),( Jeong Su Byeon ),( Hyung Seon Jeon ),( Jong Duck Jang ),( Young Jin Yang ),( In Soo Cho ),( Sang Ho Cha ) 한국예방수의학회(구 한국수의공중보건학회) 2015 예방수의학회지 Vol.39 No.3
Bone fractures are most often seen in racetrack horses because of the high level of intensity in racing. These issues are the main cause of decreased performance in racehorses. Mesenchymal stem cells (MSCs) have been explored to improve intra-articular therapy in racehorses. MSCs are essential for the repair and regeneration of damaged tissues. In this study, the effect of intra-articular injection of MSCs in racehorses was investigated. Before accessing the MSC therapy, synovial fluids were obtained from the fracture site of racehorses, and adipose tissue was collected for MSC isolation. Using the MSC specific marker, adipose tissue-derived MSCs were identified. The racehorses received intra-articular injection of autologous MSCs (or allogeneic) (3 × 107 cells/3 mL). After 1 or 2 weeks, synovial fluids were collected from racehorses. To test the effect of MSC injection using ELISA, we analyzed inflammatory factors from the untreated samples compared to MSC-treated samples of racehorses. The level of pro-inflammatory factors (interleukin-1β and prostaglandin E2) was significantly decreased in synovial fluids of MSC-injected racehorses, compared to before accessing the MSC therapy, whereas, the level of anti-inflammatory factor (interleukin-10) was higher than prior to accessing the MSC therapy. Further studies are needed to investigate the anti-inflammatory mechanism of MSC in racehorses.
Lee, Jienny,Byeon, Jeong Su,Lee, Keum Sil,Gu, Na-Yeon,Lee, Gyeong Been,Kim, Hee-Ryang,Cho, In-Soo,Cha, Sang-Ho Springer-Verlag 2016 Veterinary research communications Vol.40 No.1
<P>Mesenchymal stem cells (MSCs) have the ability to differentiate into multi-lineage cells, which confers great promise for use in regenerative medicine. In this study, canine adipose MSCs (cAD-MSCs) were isolated from canine adipose tissue. These cells clearly represented stemness (Oct4, Sox2, and Nanog) and differentiation potential into the mesoderm (adipocytes, chondrocytes, and osteoblasts) at early passages. The aim of this study was to evaluate the effects of hypoxia on the differentiation potential into mesoderm, and the expression of anti-apoptotic genes associated with cell survival for the optimal culturing of MSCs. We observed that the proliferation of the cAD-MSCs meaningfully increased when cultured under hypoxic condition than in normoxic condition, during 7 consecutive passages. Also, we found that hypoxia strongly expressed anti-senescence related genes such as HDAC1 (histone deacetylase 1), DNMT1 (DNA (cytosine-5)-methyltransferase 1), Bcl-2 (inhibitor of apoptosis), TERT (telomerase reverse transcriptase), LDHA (lactate dehydrogenase A), SLC2A1 (glucose transporter), and DKC1 (telomere holoenzyme complex) and differentiation potential of cAD-MSCs into chondrocytes, than seen under the normoxic culture conditions. We also examined the multipotency of hypoxic conditioned MSCs using quantitative real-time RT-PCR. We found that the expression levels of stemness genes such as Oct-4, Nanog, and Sox-2 were increased in hypoxic condition when compared to the normoxic condition. Collectively, these results suggest that hypoxic conditions have the ability to induce proliferation of MSCs and augment their chondrogenic potential. This study suggests that cell proliferation of cAD-MSC under hypoxia could be beneficial, when considering these cells for cell therapies of canine bone diseases.</P>
Lee, Min-Kyung,Park, Hee-Jung,Kwon, Sung-Ho,Jung, Ye-Jin,Lyu, Ha-Na,Lee, Do-Gyeong,Song, Na-Young,Choi, Hong-Keun,Park, Sangkyu,Baek, Nam-In,Lee, Youn-Hyung 한국응용생명화학회 2013 Applied Biological Chemistry (Appl Biol Chem) Vol.56 No.1
Principal components of aquatic plants responsible for inhibiting the growth of Microcystis aeruginosa, a blue-green alga (cyanobacterium) were determined. Methanol extracts of Brasenia schreberi showed strong inhibitory activity against the growth of the cyanobacterium. Two purified flavonoids were afforded through repeated column chromatographies. The compounds were determined as quercetin (1) and quercetin 3-O-(6"-galloyl)-${\beta}$-D-glucopyranoside, tellimoside (2) by the interpretation of spectroscopic data including NMR, MS, and IR. Compounds 1 and 2 were first isolated from Brasenia schreberi. In addition, compound 2 showed very strong inhibitory activity against the growth of M. aeruginosa.
Highly Pathogenic Avian Influenza A(H5N6) in Domestic Cats, South Korea
Lee, KyungHyun,Lee, Eun-Kyoung,Lee, HyunKyoung,Heo, Gyeong-Beom,Lee, Yu-Na,Jung, Ji-Youl,Bae, You-chan,So, ByungJae,Lee, Youn-Jeong,Choi, Eun-Jin U.S. Department of Health and Human Services * Cen 2018 Emerging Infectious Diseases Vol.24 No.12
<P>In December 2016, highly pathogenic avian influenza (HPAI) infection with systemic pathologic lesions was found in cats in South Korea. Genetic analyses indicated that the feline isolates were similar to HPAI H5N6 viruses isolated in chicken farms nearby. This finding highlights the need for monitoring of domestic mammals during HPAI outbreaks.</P>
Evaluation of the zoonotic potential of multiple subgroups of clade 2.3.4.4 influenza A (H5N8) virus
Lee, Yu-Na,Lee, Eun-Kyoung,Song, Byung-Min,Heo, Gyeong-Beom,Woo, Sang-Hee,Cheon, Sun-Ha,Lee, Youn-Jeong 3M Company 2018 Virology Vol.516 No.-
<P><B>Abstract</B></P> <P>Clade 2.3.4.4 H5N8 highly pathogenic avian influenza viruses (HPAIVs) have spread worldwide. Phylogenetic analysis identified two genetic groups of the H5N8 HPAIVs in South Korea; group A evolved further into four subgroups. Here, we examined the zoonotic potential, both in vivo and in vitro, of genetically distinct subgroups of H5N8 HPAIVs isolated in South Korea. When compared with other subgroups, A/mallard/Korea/H2102/2015 (H2102) virus caused relatively severe disease in mice at high doses. In ferrets, all H5N8 viruses replicated restrictively in the respiratory tract and did not induce significant clinical signs of influenza infection. <I>In vitro</I> studies, all viruses displayed a hemagglutinin phenotype that was poorly adapted for infection of mammals, although the H2102 virus exhibited higher replication kinetics at 33°C than the others. Although H5N8 HPAIVs have not yet acquired all the characteristics required for adaptation to mammals, their ability to evolve continuously underscores the need for timely risk assessment.</P> <P><B>Highlights</B></P> <P> <UL> <LI> H2102 HPAIV (H5N8) caused relatively severe disease in mice at high doses. </LI> <LI> All H5N8 viruses replicated restrictively in the respiratory tract of ferrets. </LI> <LI> All H5N8 viruses displayed a HA phenotype that was poorly adapted to mammals. </LI> <LI> H2102 HPAIV (H5N8) showed higher replication kinetics at 33°C than other strains. </LI> <LI> H5N8 HPAIVs have not yet acquired all features required for mammalian adaptation. </LI> </UL> </P>
Characterization and Genomic Analysis of Novel Bacteriophage ΦCS01 Targeting Cronobacter sakazakii
( Gyeong-hwuii Kim ),( Jaegon Kim ),( Ki-hwan Kim ),( Jin-sun Lee ),( Na-gyeong Lee ),( Tae-hyun Lim ),( Sung-sik Yoon ) 한국미생물생명공학회(구 한국산업미생물학회) 2019 Journal of microbiology and biotechnology Vol.29 No.5
Cronobacter sakazakii is an opportunistic pathogen causing serious infections in neonates. In this study, a bacteriophage ΦCS01, which infects C. sakazakii, was isolated from swine feces and its morphology, growth parameters, and genomic analysis were investigated. Transmission electron microscopy revealed that ΦCS01 has a spherical head and is 65.74 nm in diameter with a 98.75 nm contracted tail, suggesting that it belongs to the family Myoviridae. The major viral proteins are approximately 71 kDa and 64 kDa in size. The latent period of ΦCS01 was shown to be 60 min, and the burst size was 90.7 pfu (plaque-forming units)/ infected cell. Bacteriophage ΦCS01 was stable at 4-60°C for 1 h and lost infectivity after 1 h of heating at 70°C. Infectivity remained unaffected at pH 4-9 for 2 h, while the bacteriophage was inactivated at pH < 3 or >10. The double-stranded ΦCS01 DNA genome consists of 48,195 base pairs, with 75 predicted open reading frames. Phylogenetic analysis is closely related to that of the previously reported C. sakazakii phage ESP2949-1. The newly isolated ΦCS01 shows infectivity in the host bacterium C. sakazakii, indicating that it may be a promising alternative to antibacterial agents for the removal of C. sakazakii from powdered infant formulas.