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Ku, Bo Mi,Yune, Young Phil,Lee, Eun Shin,Hah, Young-Sool,Park, Jae Yong,Jeong, Joo Yeon,Lee, Dong Hoon,Cho, Gyeong Jae,Choi, Wan Sung,Kang, Sang Soo The Korean Society of Developmental Biology 2013 발생과 생식 Vol.17 No.4
Transforming growth factor (TGF) family is well known to induce the chondrogenic differentiation of mesenchymal stem cells (MSC). However, the precise signal transduction pathways and underlying factors are not well known. Thus the present study aims to evaluate the possible role of C2 domain in the chondrogenic differentiation of human mesenchymal stem cells. To this end, 145 C2 domains in the adenovirus were individually transfected to hMSC, and morphological changes were examined. Among 145 C2 domains, C2 domain of protein kinase C eta ($PKC{\eta}$) was selected as a possible chondrogenic differentiation factor for hMSC. To confirm this possibility, we treated $TGF{\beta}3$, a well known chondrogenic differentiation factor of hMSC, and examined the increased-expression of glycosaminoglycan (GAG), collagen type II (COL II) as well as $PKC{\eta}$ using PT-PCR, immunocytochemistry and Western blot analysis. To further evaluation of C2 domain of $PKC{\eta}$, we examined morphological changes, expressions of GAG and COL II after transfection of $PKC{\eta}$-C2 domain in hMSC. Overexpression of $PKC{\eta}$-C2 domain induced morphological change and increased GAG and COL II expressions. The present results demonstrate that $PKC{\eta}$ involves in the TGF-${\beta}3$-induced chondrogenic differentiation of hMSC, and C2 domain of $PKC{\eta}$ has important role in this process.
Caffeine inhibits cell proliferation and regulates PKA/GSK3β pathways in U87MG human glioma cells
Ku, Bo Mi,Lee, Yeon Kyung,Jeong, Joo Yeon,Ryu, Jinhyun,Choi, Jungil,Kim, Joon Soo,Cho, Yong Woon,Roh, Gu Seob,Kim, Hyun Joon,Cho, Gyeong Jae,Choi, Wan Sung,Kang, Sang Soo Springer-Verlag 2011 Molecules and cells Vol.31 No.3
Caffeine Inhibits Cell Proliferation and Regulates PKA/GSK3β Pathways in U87MG Human Glioma Cells
Bo Mi Ku,Yeon Kyung Lee,Joo Yeon Jeong,Jinhyun Ryu,Jungil Choi,Joon Soo Kim,Yong Woon Cho,Gu Seob Roh,Hyun Joon Kim,Gyeong Jae Cho,Wan Sung Choi,Sang Soo Kang 한국분자세포생물학회 2011 Molecules and cells Vol.31 No.3
Caffeine is the most commonly ingested methylxanthine and has anti-cancer effects in several types of cancer. In this study, we examined the anti-cancer effects of caffeine on gliomas, both in vitro and in vivo. In vitro, caffeine treatment reduced glioma cell proliferation through G_0/G_1-phase cell cycle arrest by suppressing Rb phosphorylation. In addition, caffeine induced apoptosis through caspase-3activation and poly(ADP-ribose) polymerase (PARP) cleavage. Caffeine also phosphorylated serine 9 of glycogen synthase kinase 3 beta (GSK3β). Pretreatment with H89, a pharmacological inhibitor of protein kinase A (PKA), was able to antagonize caffeine-induced GSK3β^(ser9) phosphorylation,suggesting that the mechanism might involve a cAMP-dependent PKA-dependent pathway. In vivo, caffeine-treated tumors exhibited reduced proliferation and increased apoptosis compared with vehicle-treated tumors. These results suggest that caffeine induces cell cycle arrest and caspase-dependent cell death in glioma cells,supporting its potential use in chemotherapeutic options for malignant gliomas.
Ryu, Jinhyun,Ku, Bo Mi,Lee, Yeon Kyung,Jeong, Joo Yeon,Kang, Seokmin,Choi, Jungil,Yang, Yeongae,Lee, Dong Hoon,Roh, Gu Seob,Kim, Hyun Joon,Cho, Gyeong Jae,Choi, Wan Sung,Kim, Nayoung,Kang, Sang Soo Potamitis Press 2011 Anticancer research Vol.31 No.12
<P>High invasiveness of glioma cells is one of the reasons that patients with malignant glioma have a poor prognosis. Resveratrol, a plant compound abundant in the peel of grapes, has been suggested as a potential cancer chemopreventive agent. Therefore, we investigated the effect of resveratrol on glioma cell invasion.</P>
Jeong, Joo-Yeon,Ku, Bo-Mi,Lee, Yeon-Kyung,Ryu, Jin-Hyun,Choi, Jung-Il,Kim, Joon-Soo,Cho, Yong-Woon,Roh, Gu-Seob,Kim, Hyun-Joon,Cho, Gyeong-Jae,Choi, Wan-Sung,Kang, Sang-Soo The Korean Society for Integrative Biology 2011 Animal cells and systems Vol.15 No.3
In the present study, we examined the effects of caffeine on food intake and body weight, and pro-opiomelanocortin (POMC) and agouti-related protein (AgRP) expression in the hypothalamus. Rats were administered intraperitoneally with 100 mg/kg caffeine (a high, non-toxic dose) or saline during the light phase. Intraperitoneal administration of caffeine induced a significant reduction in food intake and body weight 12 hr after treatment. In addition, POMC expression was significantly increased and AgRP expression was decreased in the arcuate nucleus (Arc) after caffeine treatment. These results demonstrate that administration of caffeine up-regulates POMC expression and down-regulates AgRP expression in the Arc, suggesting that the activation of the hypothalamic POMC neurons and inhibition of the AgRP neurons might play a role in the regulation of food intake and body weight by caffeine.
( Ghee Hwan Kim ),( Mi Jeong Kang ),( Keum Han Noh ),( Do Gyeong Oh ),( Won Ku Kang ),( Hye Gwang Jeong ),( Kwang Youl Lee ),( Han Gun Kim ),( Hyung Sik Kim ),( Tae Cheon Jeong ) 영남대학교 약품개발연구소 2015 영남대학교 약품개발연구소 연구업적집 Vol.25 No.-
To investigate the nephrotoxic potential of melamine (MEL) and cyanuric acid (CA) in male Sprague-Dawley rats,7-d repeated-dose studies were performed. The experimental groups of MEL100 and CA100 were orally administered with MEL and CA at 100 mg/kg/d for 7 d, respectively. In groups dosed with MEL-CA mixtures, melamine and cyanuric acid (1:1) were simultaneously administered at 4, 20, or 100 mg/kg/d for 7 d (i.e., MEL-CA4, MEL-CA20, or MEL-CA100, respectively). Body weights were not markedly affected in MEL100, CA100, and MEL-CA4 groups, but significantly reduced in MEL-CA 20 and 100 rats. Most parameters deter-mined in sera and tissues were not markedly altered in MEL100, CA100, and MEL-CA4-treated rodents, However, BUN, creatinine, total protein, and kidney weights were significantly increased in MEL-CA20-and MEL-CA100-treated animals. Renal histopathologic findings also revealed signs of toxicity, including tubular dilatation, crystal deposition, granulomatous tubu-lo-interstitial inflammation, and tubular necrosis with regeneration. Data suggested that the combination of MEL and CA might be responsible for observed nephrotoxicity that was not seen following individual exposure to either MEL or CA alone. Subsequently, the concentra-tions of MEL and CA were determined in serum, urine, and kidney tissues by using liquid chromatography-mass spectrometry. Toxicokinetic studies indicated that MEL or CA alone might be eliminated almost completely within 24 h after dosing showing no accumulation in kidney. However, the combined MEL-CA dose produced marked accumulation of chemicals in blood and kidneys. These results suggested that combined MEL and CA might produce renal toxicity due to significant chemical accumulation in kidney accompanied by low excretion.
( Ghee Hwan Kim ),( Mi Jeong Kang ),( Keum Han Noh ),( Do Gyeong Oh ),( Won Ku Kang ),( Hye Gwang Jeong ),( Kwang Youl Lee ),( Han Gun Kim ),( Hyung Sik Kim ),( Tae Cheon Jeong ) 전남대학교 약품개발연구소 2014 약품개발연구지 Vol.23 No.-
To investigate the nephrotoxic potential of melamine (MEL) and cyanuric acid (CA) in male Sprague-Dawley rats, 7-d repeated-dose studies were performed. The experimental groups of MEL100 and CAl00 were orally administered with MEL and CA at 100 mg/kg/d for 7 d, respectively. In groups dosed with MEL-cA mixtures, melamine and cyanuric acid (1:1) were simultaneously administered at 4, 20, or 100 mg/kg/d for 7 d (i.e., MEL-CA4, MEL-CA20, or MEL-CA 100, respectively). Body weights were not markedly affected in MEL 100, CA 100, and MEL-CA4 groups, but significantly reduced in MEL-CA 20 and 100 rats. Most parameters determined in sera and tissues were not markedly altered in MEL 100, CA 100, and MEL-CA4-treated rodents. However, BUN, creatinine, total protein, and kidney weights were significantly increased in MEL-CA20- and MEL-CA100-treated animals. Renal histopathologic findings also revealed signs of toxicity, including tubular dilatation, crystal deposition, granulomatous tubulo-interstitial inflammation, and tubular necrosis with regeneration. Data suggested that the combination of MEL and CA might be responsible for observed nephrotoxicity that was not seen following individual exposure to either MEL or CA alone. Subsequently, the concentrations of MEL and CA were determined in serum, urine, and kidney tissues by using liquid chromatography-mass spectrometry. Toxicokinetic studies indicated that MEl or CA alone might be eliminated almost completely within 24 h after dosing showing no accumulation in kidney. However, the combined MEL-CA dose produced marked accumulation of chemicals in blood and kidneys. These results suggested that combined MEL and CA might produce renal toxicity due to significant chemical accumulation in kidney accompanied by low excretion.
돼지에서 감염의 조기진단 지표로서의 Haptoglobin 적용에 관한 연구
문진산,남향미,구복경,주이석,정석찬,김종염,박용호,Moon, Jin-san,Nam, Hyang-mi,Ku, Bok-gyeong,Joo, Yi-seok,Jung, Suk-chan,Kim, Jong-yeom,Park, Yong-ho 대한수의학회 1996 大韓獸醫學會誌 Vol.36 No.3
Sera of pigs with clinically normal and infectious conditions were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) to demonstrate the specific changes in protein profile. In the sera from pigs with infection, haptoglobin with a 40KDa protein was found to be increased as compared to that of sera from normal pigs. As a rapid detection method for monitoring infections at large-scale farms, one of acute phase protein, haptoglobin, was selected to compare the concentrations between infectious and non-infectious conditions. Haptoglobin concentrations were low in pigs with clinically normal conditions but significantly increased in pigs with Aujesky's disease, hog cholera and parvo-virus infection. The studies provide that haptoglobin can be used as an indicator to monitor infections early at farm level.
돼지 단독균 인공감염 및 항생제 치료에 따른 Haptoglobin치의 변화
문진산,남향미,구복경,주이석,정석찬,김종염,박용호,Moon, Jin-san,Nam, Hyang-mi,Ku, Bok-gyeong,Joo, Yi-seok,Jung, Suk-chan,Kim, Joang-yeom,Park, Yong-ho 대한수의학회 1996 大韓獸醫學會誌 Vol.36 No.3
The changes of concentration of serum haptoglobin, body temperature, hematological result and antibody titers were compared in pigs given intravenously injection of E rhusiopathiae and control pigs. Pigs administered with E rhusiopathiae injections showed acute septicemia and arthritis. Also, statistically significant changes observed in the total white cell counts and body temperature. An increased haptoglobin concentration in serum was detected after E rhusiopathiae infection, but not during antibiotics treatment. The results indicated that measurement of the concentration of serum haptoglobin may be useful in the early diagnosis of infectious disease, such as Erysipelas.