MUC16 facilitates cervical cancer progression via JAK2/STAT3 phosphorylation‑mediated cyclooxygenase‑2 expression

Hui Shen,Meng Guo,Lu Wang,Xinyue Cui 한국유전학회 2020 Genes & Genomics Vol.42 No.2

Objectives MUC16 (mucin 16, also known as CA-125, cancer antigen 125, carcinoma antigen 125, or carbohydrate antigen 125) has been predicted as tumor biomarker for therapy. We determined to investigate effects and regulatory mechanism of MUC16 on cervical tumorigenesis. Methods Expression levels of MUC16 in cervical cancer cell lines was analyzed via qRT-PCR (quantitative real-time polymerase chain reaction). Knockdown of MUC16 was conducted via shRNA (Short hairpin RNA) transfection. MTT and colony formation assays were used to investigate effect of MUC16 on cell proliferation. Wound healing assay was utilized to detect migration and transwell assay to detect invasion. The underlying mechanism was demonstrated via western blot analysis. Results MUC16 was elevated in cervical cancer cell lines. MUC16 knockdown inhibited cell proliferation, invasion and migration. Gain- and loss-of functional assays revealed that over-expression of MUC16 activated Janus Kinase 2 (JAK2)/ signal transducer and activator of transcription 3 (STAT3) via phosphorylation, thus facilitating cyclooxygenase-2 (COX-2) expression, while knockdown of MUC16 demonstrated the reverse effect on JAK2/STAT3 activation and COX-2 expression. Moreover, inhibition of JAK2/STAT3 attenuated the regulation of MUC16 on COX-2. Conclusions MUC16 enhanced proliferation and invasion of cervical cancer cells via JAK2/STAT3 phosphorylation-mediated cyclooxygenase-2 expression, suggesting the potential therapeutic target ability of MUC16 to treat cervical cancer.

A novel design method for improving collapse resistances of multi-story steel frames with unequal spans using steel braces

Zheng Tan,Wei-hui Zhong,Bao Meng,Shi-chao Duan,Hong-chen Wang3,Xing-You Yao,Yu-hui Zheng 국제구조공학회 2023 Steel and Composite Structures, An International J Vol.47 No.2

The bearing capacities resisted by the two-bay beams of multi-story planar frames with unequal spans under column removal scenarios differ considerably owing to the asymmetric stress on the left and right beams connected to the failed column and cause the potential for beams with larger span-to-depth ratios to be unable to exert effectively, which is disadvantageous for resisting the vertical load in unequal-span frame structures. To address this problem, the structural measure of adding braces to the weak bays of multi-story unequal-span frames was proposed, with the objective of achieving a coordinated stress state in two-bay beams with unequal spans, thereby improving the collapse resistance of unequal-span frame structures. Before conducting the numerical simulation, the modeling methods were verified by previous experimental results of two multi-story planar frames with and without steel braces. Thereafter, the effects of the tensile and compressive braces on the collapse behavior of the frame structures were elucidated. Then, based on the mechanical action laws of the braces throughout the collapse process, a detailed design method for improving the collapse resistance of unequal-span frame structures was proposed. Finally, the proposed design method was verified by using sufficient example models, and the results demonstrated that the design method has good application prospects and high practical value.

Inhibition of Microcystis aeruginosa by the Extracellular Substances from an Aeromonas sp

( Yu Mei Liu ),( Ming Jun Chen ),( Meng Hui Wang1 ),( Rui Bao Jia ),( Li Li ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.9

Growth of Microcystis aeruginosa could be inhibited significantly within 24 h by the extracellular substances prepared from Aeromonas sp. strain FM. During the treatment, the concentration of extracellular soluble carbohydrates increased significantly in algal culture. Morphological and ultrastructural changes in M. aeruginosa cells, including breakage of the cell surface, secretion of mucilage, and intracellular disorganization of thylakoids, were observed. HPLC-MS analysis showed that the extracellular substances of Aeromonas sp. strain FM were a mixture of free amino acids, tripeptides, and clavulanate. Among these, the algaelysis effects of lysine and clavulanate were confirmed.

Detection of HF-ERW Status by Neural Network on Imaging

Hui-Feng Wang,Jing Cao,Xiangmo Zhao,Xiao-Meng Wang,Gui-Ping Wang 한국정밀공학회 2017 International Journal of Precision Engineering and Vol.18 No.7

To achieve online testing of high-frequency electric resistance welding (HF-ERW) tube quality, forecasting models were established for welding defect conditions with collected high-speed images of the joint melting phenomenon, based on a radial basis function neural network (RBFNN). Firstly, the dimensions of the collected image samples were deduced by principal component analysis (PCA). Then, the reduced-dimension image samples were set as inputs of both BPNN (back-propagation neural network) and, for comparison, RBFNN, which were trained so that the model parameters were optimized. Finally, the testing sample set was identified by trained networks. The experimental results show that RBFNN had better generalization ability for HF-ERW images than BPNN, which meant that the recognition rate of low-heat input status reached 100%, while the recognition rate of overheating input status reached 97.67%. They also show that the welding quality detection system based on a neural network is very effective and has a strong guiding significance for welding quality control.

Integrated Analysis Method of Thin-Walled Turbine Blade Precise Machining

Hui Wang,Lijiang Huang,Chao Yao,Meng Kou,Wenyu Wang,Bohao Huang,Weizhen Zheng 한국정밀공학회 2015 International Journal of Precision Engineering and Vol. No.

Conventional turbine blade machining technology has to face some challenges on production efficiency, quality and environmental impact. So a new machining technical solution for thin-walled turbine blade is presented. Technical problems associated with the precise machining of turbine blade are discussed in detail. On the basis of analysis with existing demerits, we propose a new fixture optimization and precise machining schema for turbine blade. In the proposed technical methodology, geometric locating tolerance analysis and FEM based structural deformation prediction methods are integrated into one framework to improve the machining performance of thin-walled turbine blade. An automatic machining station of turbine blade is also developed. Promising technical capability of this system is demonstrated and validated by real machining experiments.

The role of the genomic mutation signature and tumor mutation burden on relapse risk prediction in head and neck squamous cell carcinoma after concurrent chemoradiotherapy

Wang Hui-Ching,Moi Sin-Hua,Chan Leong-Perng,Wu Chun-Chieh,Du Jeng-Shiun,Liu Pei-Lin,Chou Meng-Chun,Wu Che-Wei,Huang Chih-Jen,Hsiao Hui-Hua,Pan Mei-Ren,Chen Li-Tzong 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Personalized genetic profiling has focused on improving treatment efficacy and predicting risk stratification by identifying mutated genes and selecting targeted agents according to genetic testing. Therefore, we evaluated the role of genetic profiling and tumor mutation burden (TMB) using next-generation sequencing in patients with head and neck squamous cell carcinoma (HNSC). The relapse mutation signature (RMS) and chromatin remodeling mutation signature (CRMS) were explored to predict the risk of relapse in patients with HNSC treated with concurrent chemoradiotherapy (CCRT) with platinum-based chemotherapy. Patients in the high RMS and CRMS groups showed significantly shorter relapse-free survival than those in the low RMS and CRMS groups, respectively (p < 0.001 and p = 0.006). Multivariate Cox regression analysis showed that extranodal extension, CCRT response, and three somatic mutation profiles (TMB, RMS, and CRMS) were independent risk predictors for HNSC relapse. The predictive nomogram showed satisfactory performance in predicting relapse-free survival in patients with HNSC treated with CCRT.

Algicidal Activity of a Dibenzofuran-Degrader Rhodococcus sp

( Meng Hui Wang ),( Peng Peng ),( Yu Mei Liu ),( Rui Bao Jia ),( Li Li ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.2

Rhodococcus sp. strain p52, a previously isolated dibenzofuran degrader, could effectively inhibit the growth of cyanobacteria, including species of Microcystis, Anabaena, and Nodularia. When strain p52 was inoculated at the concentration of 7.7×107 CFU/ml, 93.5% of exponentially growing Microcystis aeruginosa (7.3×106 cells/ml initially) was inhibited after 4 day. The threshold concentration for its algicidal activity against M. aeruginosa was 7.7×106 CFU/ml. Strain p52 exerted algicidal effect by synthesizing extracellular substances, which were identified as trans-3-indoleacrylic acid, DL-pipecolic acid, and L-pyroglutamic acid. The effective concentrations of trans-3-indoleacrylic acid and DL-pipecolic acid against M. aeruginosa were tested to be 0.5 mg/l and 5 mg/l, respectively.

Study on Importance Function for Particle Filter

Liu Lu,Meng Yang,Shu Geng,Shu-fen Wang,Yong-hui Wang 보안공학연구지원센터 2015 International Journal of Multimedia and Ubiquitous Vol.10 No.2

As an important nonlinear filter theory, particle filter is a heated issue in domestic and foreign researches. The option of importance density is one of the key steps of particle filter algorithm. The proper option of importance density can minish the negative influence of filter algorithm caused by degeneracy problem. This paper introduces several widely-used options of importance density systemically, and analyzes their features and applied perspectives respectively. The paper also advances a comprehensive method of importance density, analyzes its technical features, explores the adjudgement and improvement of this method based on various performance, and finally puts forward the necessary further study according to the engineer requirements.

BiOCl/SnS2 Core-Shell Photocatalyst for the Degradation of Organic Pollutants

Hui Meng,Tingting Wang,Hongbin Chen,Yizhu Liu,Xiang Yu,Yi Zhu,Yuanming Zhang 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2016 NANO Vol.11 No.8

BiOCl/SnS2 core-shell heterojunction is prepared by a facile and economic hydrothermal method. The obtained BiOCl/SnS2 heterojunction displays high photocatalytic activity in the degradation of Rhodamine B (RhB). The high performance is explained by the fast separation of the photoinduced electron–hole pairs promoted by the heterojunction. The cycle life of the heterojunction is also improved compared with individual BiOCl or SnS2.

The MDM2 SNP309T>G Polymorphism Increases Bladder Cancer Risk among Caucasians: a Meta-analysis

Wang, Huai-Gao,Wu, Qing-Yun,Zhou, Hui,Peng, Xin-Sheng,Shi, Meng-Jie,Li, Jie-Mei,Zhou, Yan-Fang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.13

Published studies have evaluated associations between the MDM2 SNP309T>G polymorphism and bladder cancer susceptibility. However, these generated inconsistent results. The aim of the present investigation was to quantify the strength of association between MDM2 SNP309T>G polymorphism and bladder cancer risk by conducting a meta-analysis. We searched PubMed and Embase for related studies that had been published in English before April 1, 2014 and associations were assessed by summarizing the odds ratios (ORs) with the corresponding 95% confidence intervals (CIs). Five case-control studies with a total of 972 cases and 1,012 controls were finally identified to be eligible for the meta-analysis. Overall, the results indicated that there was no significant association between the MDM2 SNP309T>G polymorphism and bladder cancer risk (for the allele model G vs. T: OR=1.08, 95% CI 0.85-1.36, p=0.54; for the co-dominant model GG vs. TT: OR=1.20, 95% CI 0.74-1.93, p=0.46; for the dominant model GG+GT vs. TT: OR=0.98, 95% CI 0.80-1.20, p=0.83; for the recessive model GG vs. GT+TT: OR=1.20, 95% CI 0.83-1.74, p=0.33). However, on subgroup analysis by ethnicity, significant associations were found in Caucasians in three models (for the allele model G vs. T: OR=1.41, 95% CI 1.10-1.81, p=0.006; for the co-dominant model GG vs. TT: OR=2.16, 95% CI 1.28-3.63, p=0.004; for the recessive model GG vs. GT+TT: OR=2.06, 95% CI 1.31-3.22, p=0.002). In summary, the present meta-analysis provides evidence that the genotype for the MDM2 SNP309T>G polymorphism may be associated with genetic susceptibility to bladder cancer among Caucasians.