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Lídia Da Silva Pereira,Thaynã Amanda Melo Souza,Rafael Walter,Cláudia Pombo Sudré,Layrana De Azevedo Dos Santos,Gabriel Bonan Taveira,Álan Chrisley Maracahipes,Virginia Silva Carvalho,Celso Shiniti Na 한국원예학회 2021 Horticulture, Environment, and Biotechnology Vol.62 No.3
Diseases caused by phytopathogenic microorganisms are diffi cult to control and can aff ect plants at diff erent stages of theirdevelopment. Several resistance genes and antimicrobial peptides (AMPs) have been identifi ed and related to the resistanceprocess of Capsicum . In recent years, studies have shown that peppers, especially the accession UENF1381, present resistanceagainst phytopathogenic microorganisms. This work aimed identify and characterize AMPs of the leaf and root from Capsicumannuum L. UENF1381 and to analyze the inhibitory activity of the AMPs on diff erent enzyme families and valuated theinhibitory activity on Colletotrichum scovillei. Initially, self-fertilized pepper seeds were cultured for 45 days in glass fl askscontaining ½-MS medium. Then, leaves were inoculated with inoculum of Xanthomonas euvesicatoria (10 8 CFU mL −1 )or water (control). Leaf and root samples were collected at 12, 24 and 48 h after inoculation to extraction. The activity ofextracts on diff erent enzyme families was analyzed by incubating the enzymes (trypsin, α-amylase and β-1,3-glucanase)with 30 μg mL −1 of the obtained extracts. The eff ect of extracts on fungi was also tested. It was identifi ed that protein of leafand root extracts from C. annuum L. UENF1381 presented a majority of bands with a low molecular mass (6–14 kDa). Weobserved that all leaf and root extracts signifi cantly inhibited trypsin and α-amylase activity and able to signifi cantly inhibitC. scovillei growth. With this work we hope to contribute to the use of peptides as potential molecules in microbial controland in the development of new Capsicum cultivars resistant to microorganisms.