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Lídia Da Silva Pereira,Thaynã Amanda Melo Souza,Rafael Walter,Cláudia Pombo Sudré,Layrana De Azevedo Dos Santos,Gabriel Bonan Taveira,Álan Chrisley Maracahipes,Virginia Silva Carvalho,Celso Shiniti Na 한국원예학회 2021 Horticulture, Environment, and Biotechnology Vol.62 No.3
Diseases caused by phytopathogenic microorganisms are diffi cult to control and can aff ect plants at diff erent stages of theirdevelopment. Several resistance genes and antimicrobial peptides (AMPs) have been identifi ed and related to the resistanceprocess of Capsicum . In recent years, studies have shown that peppers, especially the accession UENF1381, present resistanceagainst phytopathogenic microorganisms. This work aimed identify and characterize AMPs of the leaf and root from Capsicumannuum L. UENF1381 and to analyze the inhibitory activity of the AMPs on diff erent enzyme families and valuated theinhibitory activity on Colletotrichum scovillei. Initially, self-fertilized pepper seeds were cultured for 45 days in glass fl askscontaining ½-MS medium. Then, leaves were inoculated with inoculum of Xanthomonas euvesicatoria (10 8 CFU mL −1 )or water (control). Leaf and root samples were collected at 12, 24 and 48 h after inoculation to extraction. The activity ofextracts on diff erent enzyme families was analyzed by incubating the enzymes (trypsin, α-amylase and β-1,3-glucanase)with 30 μg mL −1 of the obtained extracts. The eff ect of extracts on fungi was also tested. It was identifi ed that protein of leafand root extracts from C. annuum L. UENF1381 presented a majority of bands with a low molecular mass (6–14 kDa). Weobserved that all leaf and root extracts signifi cantly inhibited trypsin and α-amylase activity and able to signifi cantly inhibitC. scovillei growth. With this work we hope to contribute to the use of peptides as potential molecules in microbial controland in the development of new Capsicum cultivars resistant to microorganisms.
Antimicrobial peptides of the genus Capsicum: a mini review
Oliveira Arielle Pinheiro Bessiatti Fava,Resende Larissa Maximiano,Rodrigues Rosana,de Oliveira Mello Érica,Taveira Gabriel Bonan,de Oliveira Carvalho André,Gomes Valdirene Moreira 한국원예학회 2022 Horticulture, Environment, and Biotechnology Vol.63 No.4
Antimicrobial peptides (AMPs) have been characterized in various organs and plant species. However, few studies have investigated AMPs in the genus Capsicum , especially their biological activities and mechanism of action. AMPs are small proteins isolated primarily from plants and animals. AMPs are of great interest to researchers because of their potent antimicrobial activity due to their ability to interact with cell membranes. While some AMPs participate in innate immune response by providing a rapid line of defense against infection, others may bolster plant defense against certain pests and pathogens. The genus Capsicum contains a myriad of AMPs involved in plant defense with the potential for new antimicrobial molecules. In recent years, our group and others have isolated and characterized AMPs from seeds, fruits, leaves and roots of Capsicum plants. Herein, we describe the diff erent AMP families, like defensins, lipid transfer proteins (LTPs), thionins, proteinase inhibitors, and others, present in the genus Capsicum . We also discuss the current knowledge of their antimicrobial activity, mechanism of action, and possible uses as antimicrobial molecules and in plant biotechnology.
Antifungal Activity of Silver Salts of Keggin-Type Heteropolyacids Against Sporothrix spp.
Luciana Da Silva Mathias,João Carlos De Aquino Almeida,Luis César Passoni,Cristiani Miranda David Gossani,Gabriel Bonan Taveira,Valdirene Moreira Gomes,Olney Vieira-Da-Motta 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.4
Sporotrichosis is a chronic and subacute mycosis causing epidemiological outbreaks involving sick cats and humans in southeastern Brazil. The systemic disease prevails in cats and in humans, with the symptoms restricted to the skin of immunocompetent individuals. Under these conditions, the prolonged treatment of animals and cases of recurrence justify the discovery of new treatments for sporotrichosis. This work addresses the antifungal activity of silver salts of Keggin-type heteropolyacid salts (Ag-HPA salts) such as Ag3[PW12O40], Ag6[SiW10V2O40], Ag4[SiW12O40] and Ag3[PMo12O40] and interactions with the antifungal drugs itraconazole (ITC), terbinafine (TBF) and amphotericin B (AMB) on the yeast and mycelia forms of Sporothrix spp. Sporothrix spp. yeast cells were susceptible to Ag-HPA salts at minimum inhibitory concentration (MIC) values ranging from 8 to 128 μg/ml. Interactions between Ag3[PW12O40] and Ag3[PMo12O40] with itraconazole and amphotericin B resulted in higher antifungal activity with a reduction in growth and melanization. Treated cells showed changes in cell membrane integrity, vacuolization, cytoplasm disorder, and membrane detachment. Promising antifungal activity for treating sporotrichosis was observed for the Ag-HPA salts Ag3[PMo12O40] and Ag3[PW12O40], which have a low cost, high yield and activity at low concentrations. However, further evaluation of in vivo tests is still required.