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백창렬,이동건,최정현,정현화,조유경,박훈준,이승훈,박윤희,이교영,민우성,김춘추,신완식 대한감염학회 2001 감염 Vol.33 No.4
As a result of the enlarging pool of unvaccinated children and young adults, there has been an increase in measles in our countries. In these situation, it has been reported that measles associated pneumoinia is easily complicated with fatal respiratory failure, espycially in immunocompromised patients. Herein we report the case of lethal measles pneumonia after allogenic hematopoietic stem cell transplantation in adults proven by autopsy. Recently, one case of measles was encountered in 39-year-old female patients after allogenic bone marrow transplanted case (chronic myelogenous leukemia), who progressed into interstitial pneumonia pattern, despite treatment including antibiotics, immunoglobulin. The patient died of giant cell pneumonia compatible with that of measles which was comfirmed in the section of necropsy lung specimen. (Korean J Infect Dis 33:301∼309, 2001)
이원식,정태훈,김교선,이국희,유덕종,손명원 대한내과학회 1986 대한내과학회지 Vol.30 No.2
The diagnostic value of transbronchial lung biopsy(TBLB) through fiberoptic bronchoscope was studied in 61 patients with diffuse or localized lung disease in which routine clinical and laboratory examinations revealed no diagnostic clue. By the technique adequate tissue specimen for diagnosis was obtainable in 28 of 33 patients (78.8 percent) and 25 of 28(89.3 percent) with localized and diffuse lung diseases, respectively. A histological diagnosis was possible in more than 75 percent of the patients with lung cancer proved by various means. On the other hand, in patients with tuberculous lesions, diffuse or localized, the incidence of positive diagnosis was less than a half. The complications among 61 patients underwent the TBLB were pneumothorax in one and hemoptysis in three, which subsided spontaneously. These facts suggest that the TRLB is a relatively safe, and more useful in the diagnosis of cancer of the lung than in inflammatory disease.
이승훈,서용칠,구교진,현창택 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1
The purpose of this study is to propose an efficient design-VE job plan and applicable methods in each phase of design process. This is for more effective and practical use of VE in design phase. This study focuses on differences of the VE target selection phase and function analysis phase in each phase of design process. In the phase of preliminary design, conceptual sketch, size, use, or major spaces of project are selected for VE target and function assessment step is skipped because present cost and function cost cannot be produced easily in early in early phase of design. At the end of preliminary design phase, function review phase is added which consists of function satisfactory indices review step and unsatisfactory function removal step.
새로운 LacZ Gene Fusion 및 Gene Cartridge Vector의 개발에 관한 연구
박교선,이영훈,박충웅,최창진 圓光大學校 基礎自然科學硏究所 1987 基礎科學硏究誌 Vol.6 No.2
In this study, a new lacZ gene vector, plasmid PGEM3-lacZ was constucted, which could be used for the study of the gene regulation and the function of proteins. Plasmid pMC1871 was digested with restriction endonuclease Pst I and Sma I to obtain a DNA fragment with the lacZ-gene(3091 bp). Recombinant plasmid pGEM3-lacZ was constructed by inserting the lacZ gene DNA(Pst I-Sma I cut DNA; 3091 bp) of pMC1871 into the Pst I and Hinc Ⅱ sites of plasmid pGEM3. After then, Recombinant plasmid pGEM3-lacZ DNA was transformated into E. coli HB 101. pGEM3-lacZ DNA/HB 101 was screened on the NZY plate with ampicillin and X-gal(5-Bromo-4-Chloro-3-Indolyl-β-D-Galactoside) by selecting blue colonies. The restriction map of pGEM.3-1acZ DNA was constructed by the following restriction enzymes :Bgl I, Xbal I, Bam HI, EcoR I, Hind Ⅲ, Kpn I, Pst I, Sma Ⅰ, Sal Ⅰ. From the restriction map, the lacZ DNA was confirmed to be present at the expected position in the plasmid pGEM3-lacZ. pGEM3-lacZ/HB101 gives about 10 times more lacZ DNA than pMC 1871/HB101.
비정상 질확대경검사 소견을 보이는 환자에서 환상투열요법의 직접적 적용에 관한 연구
우헌탁,이철민,박교훈,조용균,최훈,김복린,이홍균 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.2
Objective: Large Loop Excision of the Transformation Zone (LLETZ) has been widely used for evaluating cervical dysplasia/cancer. We performed this study to evaluate if colposcopically suspected cervical high-grade dysplasia or cancer could be directly evaluated and treated with LLETZ. Methods: 93 women who were scheduled for colposcopic evaluation because of an abnormal cervical cytology report were included in the study in Sanggye Paik Hospital from Jan to Dec 2001. Mean age of the study population was 38.6±9.0. colposcopy directed punch biopsy was not performed and histologic diagnosis was made by LLETZ. Sensitivity and specificity of colposcopic evaluation was calculated and compared with those of cervical cytology. Results: On histopathological examination by LLETZ, 11(11.8%) patients had invasive cancers including 3(3.2%) adenocarcinomas. The sensitivity and specificity of colposcopy for the detection of cervical dysplasia or cancer was 71.7% and 72.3% respectively, wile those of cytology was 56.5% and 74.5% respectively. Conclusion: LLETZ may be adequately performed for the diagnosis and treatment of cervical dysplasia/cancer based on the results of pap smear and colposcopy without punch biopsy. Cost benefit might be anticipated while cost-effectiveness analysis is necessary in the future.
Lee, Hyungbeen,Lee, Sang Won,Lee, Gyudo,Lee, Wonseok,Nam, Kihwan,Lee, Jeong Hoon,Hwang, Kyo Seon,Yang, Jaemoon,Lee, Hyeyoung,Kim, Sangsig,Lee, Sang Woo,Yoon, Dae Sung The Royal Society of Chemistry 2018 Nanoscale Vol.10 No.2
<P>Here, we demonstrate a powerful method to discriminate DNA mismatches at single-nucleotide resolution from 0 to 5 mismatches (<I>χ</I>0 to <I>χ</I>5) using Kelvin probe force microscopy (KPFM). Using our previously developed method, we quantified the surface potentials (SPs) of individual DNA-capped nanoparticles (DCNPs, ∼100 nm). On each DCNP, DNA hybridization occurs between ∼2200 immobilized probe DNA (<I>p</I>DNA) and target DNA with mismatches (<I>t</I>DNA, ∼80 nM). Thus, each DCNP used in the bioassay (each <I>p</I>DNA-<I>t</I>DNA interaction) corresponds to a single ensemble in which a large number of <I>p</I>DNA-<I>t</I>DNA interactions take place. Moreover, one KPFM image can scan at least dozens of ensembles, which allows statistical analysis (<I>i.e.</I>, an ensemble average) of many bioassay cases (ensembles) under the same conditions. We found that as the <I>χ</I>n increased from <I>χ</I>0 to <I>χ</I>5 in the tDNA, the average SP of dozens of ensembles (DCNPs) was attenuated owing to fewer hybridization events between the <I>p</I>DNA and the <I>t</I>DNA. Remarkably, the SP attenuation <I>vs.</I> the <I>χ</I>n showed an inverse-linear correlation, albeit the equilibrium constant for DNA hybridization exponentially decreased asymptotically as the <I>χ</I>n increased. In addition, we observed a cascade reaction at a 100-fold lower concentration of <I>t</I>DNA (∼0.8 nM); the average SP of DCNPs exhibited no significant decrease but rather split into two separate states (no-hybridization <I>vs.</I> full-hybridization). Compared to complementary <I>t</I>DNA (<I>i.e.</I>, <I>χ</I>0), the ratio of no-hybridization/full-hybridization within a given set of DCNPs became ∼1.6 times higher in the presence of tDNA with single mismatches (<I>i.e.</I>, <I>χ</I>1). The results imply that our method opens new avenues not only in the research on the DNA hybridization mechanism in the presence of DNA mismatches but also in the development of a robust technology for DNA mismatch detection.</P>