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엄기동(Khee Dong Eom),정병화(Byung Hwa Jung),정봉철(Bong Chul Chung),William Slikker Jr,박종세(Jong Sei Park) 대한약학회 1992 약학회지 Vol.36 No.3
The metabolic profile of doxylamine, N,N-dimethyl-2-[1-phenyl-1-(2-pyridinyl)ethoxy] ethanamine, was determined in the human urine. The free fractions of extracts were obtained without hydrolysis, and the conjugated fractions of extracts were obtained with enzyme hydrolysis using beta-glucuronidase/arylsulfatase from Helix pomatia. The mixture of acetic anhydride/pyridine (10 : 1, v : v) was used to derivatize the urinary extracts and then analyzed by gas chromatography and mass selective detector. N-desmethyldoxylamine, doxylamine carboxylic acid, desaminohydroxydoxylamine, N, N-didesmethyldoxylamine, N-acetyl conjugates of N-desmethyl and N, N-didesmethyldoxylamine, quarternary ammonium N-glucuronide of doxylamine, N-desmethyldoxylamine N-glucuronide and unchanged doxylamine were detected in the human urine obtained after oral treatment with doxylamine succinate. N-methyl-alpha-hydroxy-2-[1-phenyl-1-(2-pyridinyl)ethoxy] ethanamine, which can be a key intermediate of this metabolism, was tentatively identified by the interpretation of its mass spectrum. In this study, we proposed the metabolic pathway of doxylamine in the human on the basis of our data of the identified metabolites of doxylamine.
GC/MS에 의한 뇨중 내인성스테로이드의 정량에 관한 연구
박종세,정봉철,엄기동,장현경,유영숙 대한내분비학회 1991 Endocrinology and metabolism Vol.6 No.3
The seventeen endogeneous steroids from human urine were simultaneously analyzed by selected ion monitoring method of GC/MS. Urine samples were collected twenty-four hours from sixty-eight normal males, thirty-four normal females and eight normal children. Urinary steroids were extracted by using XAD-2 resin, hydrolyzed with -glucuronidase / arylsulfatase from Helix pormatia andquantitatively derivatized by N-methyl-N-trimethylheptafluorobutyramide / trimethylsilylimidazole / trimethylchlorosilane mixture in order to be detected on the GC / MS. The observed concentrations of seventeen endogeneous steroids in human urine were in the range of 0.01~300 g/ml. The ratio of their precursor and product metabolite of endogeneous steroids were shown in the range of 0.05~23.5. This study will be extended further to determine normal reference values for steroid in urine for Korean. Once normal reference values are obtained, the comparison data of the quantitative results of endogeneous steroids in human urine will be used as a part of diagnostic tool for endocrine diseases. (J Kor Soc Endoornol 6:238~255, 1991)
정병화(Byung Hwa Jung),엄기동(Khee Dong Eom),유영숙(Young Soo Yoo),정봉철(Bong Chul Chung),박종세(Jong Sei Park) 대한약학회 1992 약학회지 Vol.36 No.1
The metabolic profile of triprolidine, 2-[1-(4-methylphenyl)-3-(1-pyrrolidinyl-1-propenyl)] pyridine, was determined in rat urine and bile. The free fractions of urinary and biliary extracts were obtained without hydrolysis, and the conjugated fractions of extracts were obtained with enzyme hydrolysis using beta-glucuronidase from Escherichia coli. The mixture of N-methyl-N-trimethylsilyltrifluoroacetamide/trimethylsilyl choloride(100 : 1, v/v) was used to derivatize the extracts and then anaylzed by gas chromatography/mass spectrometry. Hydroxymethyltriprolidine, hydroxytriprolidine, triporolidine carboxylic acid, dihydroxytriprolidine 1, dihydroxytriprolidine 2, oxotriprolidine carboxylic acid and unchanged triprolidine were detected in rat urine and bile, which were obtained after oral treatment with triprolidine hydrochloride. The maximum urinary excretion rate of triprolidine and hydroxymethyltriprolidine which were extracted from free fraction was at 1 to 2 hours after drug administration . Hydroxymethyltriprolidine was detected in conjugated fraction, and the maximum urinary excretion rate of that metabolite was at 2 to 3 hours in rat. In rat bile analysis, triprolidine was detected only in free fraction and its biliary excretion rate showed the maximum within 30 minutes after drug administration and decreased continuously thereafter. The excretion percentage of triprolidine and hydroxymethyltriprolidine to the initial dose of the parent drug in bile and urine of rats were all low.
박종세,정봉철,정병화,엄기동 한국응용약물학회 1993 Biomolecules & Therapeutics(구 응용약물학회지) Vol.1 No.2
The metabolic profile of triprolidine, 2-[(4-methylphenyl)-3-(1-pyrrolidinyl-1-propenyl)] pyridine, was determined. Urinary extracts obtained with enzyme hydrolysis were derivatized with MSTFA/TMSCI (N-methyl-N-trimethylsilyl trifluoroacetamide/trimethylchlorosilane) and analyzed by GC/MSD. In human urine, which were obtained after the oral administration with triprolidine, hydroxymethyltriprolidine, triprolidine carboxylic acid, oxotriprolidine carboxylic acid and unchanged triprolidine were detected. The maximum urinary excretion rate of triprolidine and hydroxymethyltriprolidine which were extracted from human urine was at 2 to 4 hours after the drug administration. Triprolidine and hydroxymethyl triprolidine were identified by comparison with authentic standards in chromatographic and mass spectral properties. Triprolidine carboxylic acid was detected as a major metabolite of its metabolites in the urine. Oxotriprolidine carboxylic acid and triprolidine carboxylic acid were tentatively identified by the interpretation of its mass spectral patterns. These data suggest that in human, hydroxylation of either the benzyl or pyrrolidine ring can occur during triprolidine elimination.
Kang, Homan,Yim, Joonhyuk,Jeong, Sinyoung,Yang, Jin-Kyoung,Kyeong, San,Jeon, Su-Ji,Kim, Jaehi,Eom, Khee Dong,Lee, Hyunmi,Kim, Hye-In,Jeong, Dae Hong,Kim, Jong-Ho,Lee, Yoon-Sik American Chemical Society 2013 ACS APPLIED MATERIALS & INTERFACES Vol.5 No.24
<P>To impart a desired optical property to metal nanoparticles (NPs) suitable for surface-enhanced Raman scattering (SERS) applications, it is crucial to assemble them in two or three dimensions in addition to controlling their size and shape. Herein, we report a new strategy for the synthesis and direct assembly of Ag NPs on silica nanospheres (AgNPs-SiNS) in the presence of poly(ethylene glycol) (PEG) derivatives such as PEG-OH, bis(amino)-PEGs (DA-PEGs), and <I>O</I>,<I>O</I>′-bis(2-aminopropyl)PEG (DAP-PEG). They exhibited different effects on the formation of Ag NPs with variable sizes (10–40 nm) and density on the silica surface. As the molecular weight (MW) of DA-PEGs increased, the number of Ag NPs on the silica surface increased. In addition, DAP-PEG (MW of 2000), which has a 2-aminopropyl moiety at both ends, promoted the most effective formation and assembly of uniform-sized Ag NPs on a silica surface, as compared to the other PEG derivatives with the same molecular weight. Finally, we demonstrated that AgNPs-SiNS bearing 4-fluorobenzenethiol on its surface induced the strong SERS signal at the single-particle level, indicating that each hybrid particle has internal hot spots. This shows the potential of AgNPs-SiNS for SERS-based sensitive detection of target molecules.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/aamick/2013/aamick.2013.5.issue-24/am404435d/production/images/medium/am-2013-04435d_0008.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/am404435d'>ACS Electronic Supporting Info</A></P>