《 難經 》의 臟腑虛實에 따른 鍼灸補瀉法에 關한 硏究 : 體質針 原理에 關한 硏究(Ⅰ)

윤종화,김주경,손성철 대한침구학회 2001 대한침구의학회지 Vol.18 No.6

Obejective : Based on 《sixty-ninth Nan》 (《難經·六十九難》) the interpromoting of the five element's balance method to discuss following "when deficiency than should strengthen mother and when excessive than eliminate son" theories. Method : This strengthening and eliminating method is based on Jang-bu organ's balance method when "east is excessive (liver excessive (肝實 )) and west is deficiency (lung deficiency (肺虛))" then "eliminate the south and strengthen the north "'s method. Results : The seventy-fifth Nan (《難經·七十五難》) explains" son effects mother's excessive and mother effects son's deficiency " theory where it balance method of the inter-overacting five elements controlling Jang-bu organ's differentiations. The eighty-first Nan (《難經·八十一難》) explains strengthening-eliminating method of "lung excessive and liver deficiency." Since there are two different perspective of seventy-fifth and eighty-first Nan, we must compare and discuss to make right point of view. Conclusion : the treatment method in 《Nan kyoung sixty ninth nan》 could be understood as a view of five element constitutional theory (五行體質理論), the treatment method in 《Nan kyoung seventyty-fifth nan》 of eleminating fire and strengthning water in case of liver excess and lung defficiency and the treatment method in 《Nan kyoung eighty-first nan》 of strengthning liver and eleminating lung in case of lung excess and liver defficiency could be understood as a view of the yin-yan constitutional theory (陰陽體質理論).

인삼 Peroxidase(PgPrx3) 유전자의 분리 및 연초의 형질전환

손화,심주선,김세영,노영덕,김무성,양덕춘 경희대학교식량자원개발연구소 2005 硏究論文集 Vol.24 No.-

Peroxidase는 항산화에 관련된 효소이며 식물의 환경스트레스와 성장에 관련되는 중요한 유전자이다. 본 실험에서 사용된 Peroxidase Ⅲ의 cDNA는 Panax ginseng C.A. Meyer 잎에서 추출하였으며 PgPrx3라 명명하였다. 이 gene의 ORF영역은 1,065 bP이고 355개의 아미노산을 가지고 있다. BioEdit 프로그램을 이용하여 PgPrx3와 다른 식물의 peroxidase gene과 비교한 결과 상동성을 가지고 있었으며, Spinacia oleracea(70%), Vigna angulais(71%), Nicotiana tabacum(69%) and Linum usitatissimum(65%). 그중 Vigna angularis의 상동성이 가장 높았다. 담배에 본 유전자를 형질전환시켜 유전자의 특성을 검정하기 위하여 35S/35S/AMV/peroxidas/Tnos를 벡터 pRD400에 재조합 후 아그로박테리아를 이용하여 담배에 도입시켰다. 그리고 PCR를 이용하여 유전자 PgPrx3가 담배에 도입된 것을 확인하였으며, RT-PCR로 정상적으로 PgPrx3 유전자가 전사되어 RNA를 생성하고 있음을 확인하였다. A peroxidase (E.C. 1.11.1.7) is very important enzyme as antioxidants. The function of this gene is connected with growth and environmental stress of plant. A class Ⅲ peroxidase cDNA was isolated from the leaf of Panax ginseng C.A. Meyer and named as PgPrx3 which is consisted of an ORF (open reading frame) of 1,065 bp and an amino acid of 355 residues. BioEdit software was used to compare the PgPrx3 amino acid sequence with other plants that showed homologies with Spinacia oleracea (70%), Vigna angularis (71%), Nicotiana tabacum (69%) and Linum usitatissimum (65%). The peroxidase of Vigna angulatis was the most homologous with ginseng. The chimeric PgPrx3 gene, 35S/35S/AMV/peroxidas/ Tnos, was constructed in the binary vector pRD400. Agrobacterium tumefaciens strain LBA4404 harboring disarmed Ti-plasmid was used to transform Nicotiana tabacum L. using the leaf disc. Incorporation of the chimeric gene PgPrx3 into plants were confirmed by PCR analysis of genomic DNA and RT-PCR analysis of mRNA from transgenic tobacco. The PgPrx3 gene from ginseng was stably expressed in transgenic tobacco plants.

노인환자와 중년환자의 병원음식 배식량에 대한 만족도와 섭취율 비교연구

손주현,천종희 한국식생활문화학회 2002 韓國食生活文化學會誌 Vol.17 No.5

The purpose of the study was to compare the satisfaction level on hospital meal size and actual intake rate of hospital foods between elderly and middle aged patients. Sixty one middle aged and one hundered thirty two elderly patients were surveyed on the foods served in the hospital-rice, soup, meat/fish, vegetable, kimchi and daily products. Compared to the middle aged patients, the more elderly thought the serving size of rice was too big(p<0.05), and those of soup, meat/fish and kimchi tended to be too big. There was no difference in the satisfaction level on the serving size of vegetable dish between two age groups. · In actual intake rates of hospital meal there were no significant differences between the elderly and middle aged patients. However, the elderly male ate significantly(p<0.05) less amount of rice than the middle aged male and the elderly female ate significantly(p<0.01) less amount of meat/fish then the middle aged female. Satisfaction levels and actual intake rates were significantly correlated in all food items.

의도적 재식술을 이용한 치관-치근 파절의 치험례

손주효,최형준,손흥규 대한소아치과학회 2000 大韓小兒齒科學會誌 Vol.27 No.2

파절의 종류는 치관파절, 동시에 나타난 치관-치근파절이 있고 치수노출에 따라 단순파절과 복잡파절로 나뉜다. 치아파절이 치은 연하로 침범하여 치아장축을 따라 나타난 경우나 치근의 1/3이상을 넘어선 경우 발치가 원칙이다. 그러나 치근쪽 파절편을 교정적으로 정출시켜서 수복시켜 주는 방법과 인위적으로 발치하여 적절하게 수복 가능한 위치로 재식시켜주는 방법을 선택적으로 이용할 수 있다. 본 증례에선 치아외상으로 상악 중절치가 치관-치근 파절된 혼합치열기의 환아에서 의도적 재식술을 이용하여 발치 후 즉일 근관치료와 역충전 후 레진수복을 시행하여 치료하였기에 보고하는 바이다. 주요어 : 의도적 재식술, 치관-치근 파절, 치은 연하 침범

國語聲調硏究의 現況과 課題

孫周一 又石大學校 1983 論文集 Vol.5 No.-

The conclusion of this study are: 1. Korean language tone is level-pitch register system with two tonemes. 2. The characteristics of Korean language tone is not true tonal language but word-pitch system. 3. Tone is not used in SOHAKEUNHAI(小學諺解: 1587) and tonal system is not appeared 17C. before or after. 4. The correlation between middle Korean tone and Kyung Sang(慶尙) dialect tone is "Both tone is the same but is not now." The detail problems are contained each chapter.

노인환자와 풍년환자의 병원 음식 만족도 비교 분석

손주현,천종희 한국식생활문화학회 2001 韓國食生活文化學會誌 Vol.16 No.5

The purpose of this study was to analyze the satisfaction level on hospital foods in elderly and middle aged patients. The subjects were selected among the patients treated in the Inha University hospital during July of 1999. One hundred thirty five elderly patients aged over sixty(68 men, 67 women) and sixty five patients aged in forties(35 men, 30 women) were surveyed through questionnaires in terms of satisfaction level on hospital foods and the food preferences. Satisfaction on general taste, saltiness, serving temperature, appearance, texture and variety of hospital foods was not significantly different according to age. However, elderly men showed significantly higher satisfaction level than elderly women in terms of general taste of hospital foods(p<0.01). The preferences of cooking method for meat, fish, vegetable and the preferred kinds of kimchi and milk & milk products were not significantly different between the two age groups. However, elderly women significantly preferred sauted meat to broiled meat compared to elderly men(p<0.05).

미백물질로써 폴리페놀 화합물의 분리 및 화학구조

김주덕,손애량 東國專門大學 1999 金龜論叢 Vol.7 No.1

우리는 미백제 개발 연구의 일환으로 한국산 지유로부터 새로운 procyanidin류를 분리하였고, thiolysis, desulfurization, methylation, tannase 처리 및 기기 분석에 의해 구조를 결정한 결과 flavan-3-ol 이량체에 gallate한 분자가 결합된 procyandin B-3 3-O-gallat였다. 이 화합물의 효소저해 효과를 관찰한 결과 tyrosinasesms 100μM에서 약 50%의 저해 효과가 있었다.

더덕의 효율적인 재분화 및 뿌리발달

심주선,조숙녀,손화,김무성,노영덕,김세영,양덕춘 경희대학교식량자원개발연구소 2005 硏究論文集 Vol.24 No.-

더덕은 식용, 관상용, 생약재료로 쓰이는 방향성 식물이다. 본 연구는, 종자의 기내배양을 통하여 식물체 재분화와 뿌리발달을 실험했다. 종자의 발아는 GA_(3) 100 mg·L(-1)처리와 저온처리 시 매우 양호하였다. 더덕의 기내에서 shoot의 형성은 2 mg·L^(-1) BA, 2 mg·L(-1) NAA가 첨가된 MS 기본배지에서 가장 양호하였으며, 줄기의 절편체와 비교하여 잎 절편체에서 재분화가 잘되었다. 또한 뿌리의 발육은 계속적인 계대배양에 의해서 양호하였는데 shoot의 길이를 1 cm 정도 남긴 뿌리를 연속적으로 4회 계대배양에서 뿌리가 주근으로 발육하였으며 길이는 약 3 cm 및 직경은 0.5 cm의 정상적인 뿌리를 얻을 수 있었다. Codonopsis lanceolata is widely used as edibles, ornamental plant and crude drug material. This study was carried out to confirm the ratio of germination and efficient differentiation of shoot and root in vitro. The germination of Codonopsis lanceolata seed was excellent in the medium with GA_(3) 100 mg·L(-1) and chilling treatment. The shoot formation of Codonopsis lanceolata was good at the MS medium with 2 mg·L(-1) BA and 2 mg·L(-1) NAA. According to inoculum part, ratio of re-differentiation was higher from the leaf explant compared with the stem explant. For root development, successive subculture with 1 cm shoot of upper part from root was efficient. Roots of Codonopsis lanceolata cultured in vitro system was grown like normal root with length of 3 cm and thickness of 0.5 cm at the 4 successive subcultures.

정상 인체피부섬유모세포종에 대한 벤조산의 독성효과

전주원,손영우,서부일,한두석 대한본초학회 2004 大韓本草學會誌 Vol.19 No.1

Objective : The aim of this study was to evaluate cytotoxic effect of benzoic acid on normal human skin fibroblast cell line , Detroit 551. Methods : The cytotoxicity was determined by colorimetric assays such as MTT assay or XTT assay. The microscopy was carried out to observe Detroit 551 cell line. Cells were incubated with various concentrations of benzoic acid in CQ incubator controlled with 37 c for 48 hours. Results : The cytotoxic effect of benzoic acid on Detroit 551 did not show a positive significance at 50 uM and 100 uM benzoic acid by MTT assay or X T T assay compared with control. MTTB and XTTg were 900.6 uM and 974.6 uM, respectively. In microscopic study, Detroit 551 treated with benzoic acid showed a slightly decreased cell number, while the morphological changes of cell was not showed. Conclusion : Above the results, the cytotoxicity of benzoic acid was midtoxicity on normal human skin fibroblasts cell line. Detrit 551

쌍태아에서 제 1태아의 분만 후 제 2태아의 지연분만 : 중례보고

김주환,서민정,유희정,노권일,조대현,박정규,이정헌,조성남,손영수 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.3

With the widespread use of fertility medications and techniques of in vitro fertilization, multiple gestation has become epidemic in modern obstetric practice. Delivery of the initial fetus in a multiple gestation usually is followed by delivery of the subsequent fetus or fetuses shortly thereafter. However in rare circumstances, the delivery is delayed for days due to disappearance of uterine contraction after delivery of the first fetus. When uterine activity ceases after the delivery of the first neonate, a policy of nonintervention may be considered in case of an immature or very premature delivery, in order to achieve a gestational age for the remaining fetus(es) more compatible with neonatal survival. We report a case of twin gestation with prolongation of the delivery interval between the twins for 46 days.