백화사설초 메탄올 추출물에 의한 HL-60 細胞 枯死過程에서의 cell cycle 關聯因子의 活性變化 硏究

한세희,이종범,문구,문석재,원진희,박래길,이종덕 대한한방종양학회 2000 대한한방종양학회지 Vol.6 No.1

Objecives: Hedyotis diffusa is used to treat cancer in traditional Korea Medicine. So this study was carried out to examine the expression of cell cycle related genes in HL-60 cells undergoing apoptosis by the methanol extract of Hedyotis diffusa. Methods: 1. HL-60 cells were treated with various concentrations (from 200 to 50㎍/ml)of metnanol extract and H20 extract (200 ㎍/ml) of hedyotis diffusa. After 48 h later, the cells were tested for viability by MTT assay. 2. The HL-60 cells were treated with 200 ㎍/ml of methanol extract for the indicated periods. The whole cell lysates were prepared and analyzed by westem blotting using anti-p53 antibody. 3. The nuclear extract were prepared and analyed by western blotting using anti-p21 antibody, anti-p27 antibody, anti-cyclen A antibody, anti-cylin E antibody and anti-CDK2 antinbody. Results: 1. The methanol extract of Hedyotis diffusa induced the death of HL-60 cells in a dose dependent manner. 2. The methanol extract of Hedyotis diffusa makedly decreased the level of p21/Cipl and cyclin A in a time dependent manner. 3. The methanol extract of Hedyotis diffusa markedly increased tje ;eve; pf p27/Kip and cyclin E in a time dependent mammer. 4.The methanol extract of Hedyotis diffusa markedly did not affect the level of CDK2. Conclusions: These results provide evidence that expression of cell cycle related genes in HL-6- cells undergoing apoptosis by the methanol extract of Hedyotis diffusa mainly results from decreased level of p21/cipl and increased level of p27/Kipl of the cell cycle related genes.

교정선의 종류, 결찰방법, 타액의 유무에 따른 마찰력의 비교연구

한정숙,이진우,차경석 대한치과교정학회 2001 대한치과교정학회지 Vol.31 No.2

치아에 부착된 bracket이 교정선을 따라 이동할때 필연적으로 bracket과 교정선, 결찰재 사이에 마찰력이 발생된다. 이에 저자는 동일한 bracket내에서 교정선의 종류, 결찰방법, 건조와 타액상태에 따른 마찰력 차이, 타액의 윤활제로서의 기능을 살펴보기 위해 본 연구를 시행하게 되었다. 본 실험에 사용된 bracket은 .018"× .025" slot의 상악 견치용 standard edgewise bracket(RMO. USA)이며, 교정선은 .016" , .016" × .022" 크기의 Cobalt-chromium(Elgiloy . RMO. USA), Nickel-titanium(Ni-Ti) (ORTHOLLOY : Goldstar Cable Co. KOREA), Beta- titanium(TMA : ORMCO Co., USA)의 2가지형태의 3종을 이용하여 활주할때의 마찰력을 만능시험기(Instron, M 1000 EC)를 사용하여 계측하여 다음과 같은 결론을 얻었다. 1.각각의 동일조건하에서 교정전의 재질에 따른 마찰력은 Co-Cr, Ni-Ti, β-Ti순으로 증가하였다. 단, 타액상태에서 elastomeric으로 결찰한 .016" 군에서는 예외이다. 2.각 조건하(건조/타액상태, .016" /.016" × .022" ) 에서 결찰방법에 따른 마찰력은 elastomeric보다 stainless steel 결찰에서 더 크게 나타났다(p<0.05). 3.각각의 교정선과 결찰방법에서 타액 유무에 따른 마찰력은 stainless steel로 결찰한 .016" Co-Cr, Ni-Ti, β-Ti는 타액상태에서 감소하나, stainless steel로 결찰한 .016" × .022" Co-Cr, Ni-Ti, β-Ti는 타액상태에서 증가하였다(p<0.05). 4.각 조건하(건조/타액상태, elastomeric/stainless steel결찰) .016" 과 .016" × .O22" 교정선 사이의 마찰력은 .016" × .022" 교정선에서 증가하였다(p<0.05). The purpose of this study was fourfold - to evaluate the general laws of friction applied to orthodontic conditions, to compare archwire materials under these controlled conditions, to compare ligation method, and to measure the effect of the artificial saliva on friction with these materials Three wire alloys (Cobalt-chromium, Nickel-titanium, Beta-titanium) in two size wires (.016" ,.016" × .022" ) were examined respect to the bracket (0.18 × .025" standard), and two ligature material (stainless steel, elastomeric) in dry and wer conditions The result were as follows, 1.The order of frictional force against alloy materials was Co-Cr (lowest), Ni-Ti, and β -Ti(highest) - with the exception of elastomeric ligation under wet conditions. 2.S.S. ligation gave rise to significantly greater friction than elastomeric ligation did. 3.Testing in the presence of saliva, rather than in dry conditions, decreased the frictional force for S.S. ligation with .016" Co-Cr, Ni-Ti, β -Ti. but, increased the frictional force for 5.5. ligation with .016" × .022" Co-Cr, Ni-Ti, β-Ti. 4.016" ×.022 " wire generated more friction than .016" wire.

建築構造計算의 電算化에 관한 硏究 : -C. M? V 算定을 中心으로-

한진석 忠州大學校 1986 한국교통대학교 논문집 Vol.20 No.1

Users of PERSONAL COMPUTER with easy process and low cost are increasing today. Then this study develop a series of program for Structural Design calculation under consideration of the following demands; 1. To make the calculation under the philosophy and judgement of designer and the consideration in the midst of calculation process possible. 2. To make the check and change of input data and reruning easy. 3. To represent the output data. Especially, this study deals with a part of C.M.V,REPORT that a preparing step for structural design.

XML기반 전자상거래 프레임워크 기술

한흥구,박진석,윤순영,최성 호서대학교 반도체제조장비국산화연구센터 2001 반도체장비학술심포지움 Vol.2001 No.-

담화분석의 방법모색과 실제

한진석 大田大學校 1984 論文集 Vol.3 No.1

하부팽창형 임프란트의 유한요소 분석 및 골형성에 대한 연구

한석환,김영규,민경기,조완석,권종진 大韓顎顔面成形再建外科學會 2003 Maxillofacial Plastic Reconstructive Surgery Vol.25 No.3

The aim of this study was to evaluate by finite element analysis influence of investigated expanding implant on stress distribution and to observe biocompatibility, bone formation feature and change in the bone contact area around the explanding implant through experimental animal study. FEA models of the mandible were constructed based on three-dimensional graphic data of the bone structure around an osseointegrated implant, analysis of stress distribution followed by 100N vertical, 50N horizontal was done. And in the animal study, expanding implant were placed in the mandibles of eight Korean mongrel dogs and analyzed the results with histomorphometric measurements. In finite element analysis, compressive stresses are produced at the bone tissue surrounding the implant, and the contact area between the implant and the bone tissue is increased, which result in increased resistance to horizontal and vertical pressure loads. In the histomorphometric analysis, the measured ratio of the contact surface and bon density between implant and bone was similar to those of the controlled group from 6 weeks. It is thought that increased horizontal and vertical resistance is a great help to the immediate loading on expanding implant and that there needs standardizing quantitative measurement of implant stability and osseointegration to determine whether or not to use the functional loading and to reduce the total treatment period reliably by placing functional loading on each implant based on both bone quality and implant stability.

연어관계를 통한 어휘덩어리 학습으로 영어 유창도의 달성

한진석 대전대학교 인문과학연구소 2001 人文科學論文集 Vol.31 No.-

타우로우루소데옥시콜린산이 흰쥐의 적출심장에서 허혈 및 재관류 손상에 미치는 영향

한석희,이우용,박진혁,이선미 성균관대학교 약학연구소 1999 成均藥硏論文集 Vol.11 No.-

Abstract - In this study, the effects of tauroursodeoxycholic acid (TUDCA) on ischemia/ reperfusion injury were investigated on isolated heart perfusion models. Hearts were perfused with oxygenated Krebs-Henseleit solution (pH 7.4, 37℃) on a Langendorff apparatus. After equilibration, isolated hearts were treated with TUDCA 100 and 200μM or vehicle (0.02% DMSO) for 10 min before the onset of ischemia in single treatment group. In 7 day pretreatment group, TUDCA 50, 100 and 200 mg/kg body weight were given orally for 7days before operation. After global ischemia (30 min), ischemic hearts were reperfused for 30 min. The physiological (i.e. heart rate, left ventricular developed pressure, coronary flow, double product, time to contracture formation) and biochemical (lactate dehydrogenase; LDH) parameters were evaluated. In vehicle-treated group, time to contracture formation was 810 sec during ischemia, LVDP was 34.0 mmHg at the endpoint of reperfusion and LDH activity in total reperfusion effluent was 34.3 U/L. Single treatment with TUDCA did not change the postischemic recovery of cardiac function, LDH and time to contracture compared with ischemic control group. TUDCA pretreatment showed the tendency to decrease LDH release and to increase time to contracture and coronary flow. Our findings suggest that TUDCA does not ameliorate ischemia/reperfusion-induced myocardial damage.

학습자중심 영어교육과 학습자 및 교사의 역할

한진석 대전대학교 인문과학연구소 1999 人文科學論文集 Vol.28 No.-

스텝이송을 이용한 미세구멍가공

한진욱,이응숙,정윤교 國立 昌原大學校 産業技術硏究所 1995 産技硏論文集 Vol.9 No.-

Micro drilling is one of the most important machining types and its importance becomes more and more increased in the whole field of industry. Micro drilling, however, has few the case of practical application, because it requests high techniques; manufacturing micro drill, treating, chip, producing precise hole shape and progressing machining efficiency. Micro drilling has a technical problem; drill breakage from drill breakage from the lack of drill stiffness and the interruption of chip. It is, therefore, essential to select of proper cutting conditions and of step fed for the method solving the lack of stiffness and the interruption of chip. Especially, study on step feed is requested more than the present. The purpose of this paper is to investigate experimentally about cutting conditions which affect on tool life and round errors and to estimate about the efficiency of step feed.