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Cryobanking of Semen, Embryo and Oocytes for Animal Industry
Ming-Che Wu,Yung-Yu Lai,Ing-Haur Hwang 한국동물생명공학회(구 한국동물번식학회) 2011 발생공학 국제심포지엄 및 학술대회 Vol.2011 No.1
Genetic resources of buffalo, cattle, goat, sheep, deer, rabbit, pig, chicken, duck, goose, turkey and swan in Taiwan are conserved in living form or frozen genetic materials. Preserving farm animals in living form must conserve a considerable number of mature individuals with reproductive potential, and continued funding and breeding space are necessary. Based on the afore‐mentioned restraints and consideration of risk diversification, living animals are preserved in Taiwan Livestock Research Institute (TLRI) branches and breeding stock reproduction grounds throughout Taiwan. Genetic materials preserved in frozen form include germ cell (sperm, egg and embryo), tissue, somatic cell, cell line, DNA and gene pool. Those preserved for long term more than 10 years are conserved in liquid nitrogen storage tank at ‒185 to ‒196℃. Animal industry on utilization of farm animal genetics emphasizes the importance of in situ conservation and considers ex situ conservation as an essential complementary activity to in situ. In situ and ex situ utilization are complementary, not mutually exclusive. The exact strategy of germplasm cryobanking will clearly depend on the conservation objectives of TLRI. In situ and ex situ strategies differ in their capacity to achieve the different bio‐utilization objectives. Based on the objectives for bio‐utilization and conservation, the existing national technical capacity and infrastructure for cryoconservation, and amount of capital to invest in developing and maintaining a gene bank for food animal genetic resources (FAnGR), each country should determine for which FAnGR, if any, national cryobanking program should be undertaken. The health and sanitary issues of animals that must be considered when establishing and operating gene banks for animal genetic resources to help prevent the conservation of potentially dangerous pathogens along with the valuable genetic material in the ultra‐low temperature cell repository. Bio‐utilization of material stored in the bio‐bank must eventually be thawed and used to create new animals and therefore, national organization and annotation of the stored material is critical to ensure its proper utilization. Stock animals are the source of semen, embryo and oocytes are usually privately owned. This ownership may or may not change during the gene banking process, but the terms of agreements between bio‐banks and donors must be explicitly defined. Taiwan Animal Germplasm Center now conserves genetic resources of 77 livestock breeds and strains, including 19 native and 38 foreign species as well as 20 new breeds produced by inbreeding in poultry or breed‐crossing with the artificial insemination in livestock. Cryobanking of semen of native animals is for genetic diversity but also for risk management to long‐term global food security. Ex situ conservation program involving in vitro storage of germplasm cryobanking can contribute to ensure that it will be available to allow livestock keepers and animal breeders to confront future changes in animal production and economic environments.
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(Yang Do Choi,(Jong Joo Cheong,(Ing Yu Hwang,(Jong Seob Lee,(Sang Ik Song,(Min Kyun Kim,(Song Yion Yeu,(Choon Kyun Jung,(Yeon Jong Koo,(Jong Tae Song,(Hak Soo Seo 한국응용생명화학회 2001 Journal of Applied Biological Chemistry (J. Appl. Vol.44 No.3
NTR1 gene of Brassica campestris L.ssp.perkinensis encodes a floral nectary-specific methyltransferase. In this study, the NTRl cDNA was expressed in E. coli to examine the enzymatic characteristics of the protein product. The GST-NTR1 fusion protein was p
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( Song Ee Park ),( Min Suk Kwon ),( Jae Hyun Tae ),( Hee Jun Kim ),( Ing Yu Hwang ),( Jung Sun Jang ),( Eun Kyung Park ) 대한내과학회 2013 대한내과학회 추계학술대회 Vol.2013 No.1
A 52-year-old female was diagnosed with ITP at our hospital in February 1987. Her Platelet count was 4×109/L at diagnosis. She was initially treated splenectomy in April 1987. Since 1987, she was intermittently administered with oral prednisolone. However, No response in her platelet count was observed. September 2009. Blood test indicated severe thrombocytopenia with a platelet count of 4×109/L, a white blood cell count of 22.39×109/L and a hemoglobin level of 12.9 g/dL. The patient was treated with intravenous immunoglobulin (IVIG, 1g/kg/day for 2 days). IVIG treatment was performed 11 times. Oral prednisone was also restarted at a dose of 1 mg /kg/day. However, No response in her platelet count was observed. Treatment with the thrombopoietin receptor (TP069O-R) agonist, eltrombopag at 50 mg/day. Despite the four months the administration Eltombopag, she fell back into the reaction after only 170,000 platelet count is 100,000 or more. Subsequently. another TPO-R agonist, romiplostim, was administered (at a starting dose of 1 μg/kg/week) in place of eltrombopag. Because of the poor response, weekly increases in the dose of romiplostim were required. An increase in the patient`s platelet count was seen at dose 3.7 μg/kg/week. The platelet continued to rapidly increase during the following weeks, up to a maximum count of 352×109/L. During treatment with romiplostim, the patient experienced no specific side effects.
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S-445 : Primary B-lymphoblastic lymphoma of gallbladder diagnoseed by laparosscopic cholecystectomy
( Song Ee Park ),( Jae Hyun Tae ),( Min Suk Kwon ),( Hee Jun Kim ),( Eun Kyung Park ),( Ing Yu Hwang ),( Jung Sun Jang ) 대한내과학회 2013 대한내과학회 추계학술대회 Vol.2013 No.1
The patient was a 72-year-old man who presented for evaluation of painless hematuria which he stated had been present for at least 1month. At the time of presentation, the patient did not report any systemic symptoms and had no fever, weight loss or dysuria. Computed tomography (CT) also showed several enhancing, sessile polyps in the gall bladder (1.5 cm and smaller). No associated stone or biliary dilation. Since no other abnormality was evident, we performed laparoscopic cholecystectomy. He was diagnosed as having B-cell lymphoblastic lymphoma (B-LBL) after surgical resection of his gall bladder (GB). A gall bladder, measuring 7.5×4.0 cm. The serosal surface is smooth and glistening. The mucosa shows four sessile polypoid lesions, measuring 1.8×1.5 cm, 1.1×0.9 cm, 0.7×0.7 cm, and 0.8×0.7 cm. The surface of the lesions is whitish and firm. No surface ulceration or hemorrhage is noted. The tumor was B-LBL LCA (+), CD20 (focal+), CD79a (+), CD3 (+), CD45RO (+), Bcl-2 (+), Ki-67 (30%). After the surgery, metastaric work-up including CT scan of the chest, positron emission tomography (PET) and bone marrow biopsy was performed. The PET revealed an increased fluorodeoxyglucose-uptake in the left maxilla, left mandible, right humerus, T12 and lt mandible mass. The clinical course suggested GB tumor was growth of the B-LBL. The definitive pathological diagnosis was B-LBL, stage IV. Systemic chemotherapy was started and subsequent response in size of the lt mandible mass. Since lymphomas of GB are exceedingly rare, the differential diagnosis would be difficult.
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