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Jeon, Hyang,Kim, Sung-Su,Kim, Yoon-Suk,Park, Yil-Sung,Kim, Yong-Hae,Choi, Sun-Ju,Kim, Soo-Kie,Kim, Tae-Ue Korean Society for Biochemistry and Molecular Biol 1998 Journal of biochemistry and molecular biology Vol.31 No.6
Echinomycin-7 is an echinomycin derivative, Smethylated sulfonium perchlorate of echinomycin. We studied the in vitro cytotoxicity and in vivo antitumor activity of echinomycin-7 against P388 leukemia cells and compared the results with echinomycin. With respect to the cytotoxic effects, echinomycin-7 had cell line-dependent $IC_{50}$ values while echinomycin had similar values to several tumor cell lines. Also, in vivo antitumor activities were observed in tumor-bearing mice treated with both agents, which showed that echinomycin-7 had a broad therapeutic dose range. We also observed the apoptosis on leukemia cells treated with echinomycin-7 which exihibited the ladder pattern of DNA on electrophoresis. In addition to apoptosis, echinomycin-7 arrested $G_1/S$ phases of the cell cycle at the same time. We then examined the signaling pathway of echinomycin-7-induced apoptosis and showed that ERK of the MAP kinase family was activated and translocated into the nucleus by echinomycin-7 stimulation. This study suggests that echinomycin-7 acts as an antitumor agent through in vitro cytotoxicity and has in vivo antitumor activity against leukemia cells, and that the echinomycin-7- induced apoptosis might involve signal transduction via MAP kinases.
Jeon, Hyo Jeong,Shin, Hea-Jin,Choi, Jeong Jin,Hoe, Hyang-Sook,Kim, Hyun-Kyu,Suh, Se Won,Kwon, Suk-Tae Elsevier 2004 FEMS microbiology letters Vol.237 No.1
<P><B>Abstract</B></P><P>The crystal structure of NAD<SUP>+</SUP>-dependent DNA ligase from <I>Thermus filiformis</I> (<I>Tfi</I>) revealed that the protein comprised four structural domains. In order to investigate the biochemical activities of these domains, seven deletion mutants were constructed from the <I>Tfi</I> DNA ligase. The mutants <I>Tfi</I>-M1 (residues 1–581), <I>Tfi</I>-M2 (residues 1–448), <I>Tfi</I>-M3 (residues 1–403) and <I>Tfi</I>-M4 (residues 1–314) showed the same adenylation activity as that of wild-type. This result indicates that only the adenylation domain (domain 1) is essential for the formation of enzyme–AMP complex. It was found that the zinc finger and helix-hairpin-helix (HhH) motif domain (domain 3) and the oligomer binding (OB)-fold domain (domain 2) are important for the formation of enzyme–DNA complex. The mutant <I>Tfi</I>-M1 alone showed the activities for in vitro nick-closing and in vivo complementation in <I>Escherichia coli</I> as those of wild-type. These results indicate that the BRCT domain (domain 4) of <I>Tfi</I> DNA ligase is not essential for the enzyme activity. The enzymatic properties of <I>Tfi</I>-M1 mutant (deleted the BRCT domain) were slightly different from those of wild-type and the nick-closing activity of <I>Tfi</I>-M1 mutant was approximately 50% compared with that of wild-type.</P>
Keratinocyte모델에서 발암성 이환방향족아민의 종(species)간 독성 감수성
전향숙,Jeon, Hyang-Suk 한국식품연구원 1999 食品技術 Vol.12 No.2
조리가공 중 생성되는 주요 발암성 이환방향족아민(heterocyclic aromatic amine)인 Trp-P-1 및 Trp-P-2가 human 및 rat keratinocytes에 대해 나타내는 세포 독성을 colony expansion법의 의해 조사, 비교하였다. 특히 Trp-P-2는 human keratinocytes에 대해서는 독성을 나타내지 않은데 반해 rat keratinocytes(계대수 2-5)에 대해서는 독성을 나타내는 선택성을 나타내었다. 이러한 Trp-P-2의 종(species)간 독성감수성 차이가 대사 효소계 활성이나 mutagenic activation상의 차이에 기인되는지를 살펴본 결과, CYP4501As 및 독성감수성 차이가 크게 나타났던 human 및 rat keratinocytes의 microsome에서 거의 같았다. 이와 같은 결과는 CYP4501A1 및 CYP1B1의 mRNA의 발현정도를 northernblot에 의해 살펴보았던 결과에서도 일치하였다. 반면 Trp-P-2의 대사활성화 및 해독화에 관여하는 효소인 N.O-acetyltransferase(NAT)활성은 rat keratinocytes보다 human keratinocytes에서 높았다. 일반적으로 독성물질의 해독화에 관여하는 glutathione S-transferase(GST) 또한 rat keratinocytes보다 human keratinocytes에서 높게 나타났다. Trp-P-2가 mutagenic metabolite로 활성화되는 정도를 salmonella microsome microsuspension assay로 살펴본 결과, 독성 감수성 차이가 크게 나타났던 human 및 rat keratinocytes간의 활성은 비슷한 것으로 나타났다. DNA 및 단백질 adduct형성능의 경우, human 및 rat keratinocytes간 DNA adduct형성능에는 차이가 없었고, 단백질 adduct형성능의 경우만 Trp-P-2에 대한 독성감수성 정도가 컸던 rat keratinocytes가 다른 세포들에 비해 크게 나타났다. 이상의 결과를 종합해 볼 때, CYP1A- 또는 CYP1B1-관련 마이크로솜 효소활성이나 mutagenic activation은 human 및 rat keratinocytes간에 나타났던 독성 감수성의 차이를 설명할 수 없으며, 해독화에 관여하는 효소활성이 종간 관찰되었던 독성 감수성의 차이에 더 중요한 역할을 하는 것으로 보인다.
( Yejoo Jeon ),( Eun Sun Jang ),( Yun Suk Choi ),( Jin-wook Kim ),( Sook-hyang Jeong ) 대한간학회 2016 Clinical and Molecular Hepatology(대한간학회지) Vol.22 No.3
Background/Aims: Glypican-3 (GPC3) protein is highly expressed in hepatocellular carcinoma (HCC) tissue. It has been suggested as a diagnostic biomarker, but its inconsistent performance means that it requires further assessment. We therefore investigated the diagnostic value of the plasma GPC3 level compared to the alpha-fetoprotein (AFP) level as a diagnostic biomarker of HCC. Methods: We enrolled 157 consecutive patients with newly diagnosed HCC and 156 patients with liver cirrhosis (LC) as the control group. GPC3 plasma levels were measured using two commercially available enzyme-linked immunosorbent assays (ELISAs, named as Assay 1 and 2), and AFP levels were measured using an enzyme-linked chemiluminescent immunoassay. The diagnostic accuracy was analyzed using the receiver operating characteristics (ROC) curve. Results: Plasma GPC3 levels in HCC patients were very low (0-3.09 ng/mL) in Assay 1, while only 3 of the 157 patients (1.9%) showed detectable GPC3 levels in Assay 2. The median GPC3 level was not significantly elevated in the HCC group (0.80 ng/mL) compared with the LC group (0.60 ng/mL). The area under the ROC curve (AUC) for GPC3 was 0.559 in Assay 1. In contrast, the median AFP level was significantly higher in HCC (27.72 ng/mL) than in LC (4.74 ng/mL), with an AUC of 0.729. Conclusions: The plasma level of GPC3 is a poor diagnostic marker for HCC, being far inferior to AFP. The development of a consistent detection system for the blood level of GPC3 is warranted. (Clin Mol Hepatol 2016;22:359-365)
( Yejoo Jeon ),( Yun Suk Choi ),( Eun Sun Jang ),( Jin Wook Kim ),( Sook-hyang Jeong ) 대한간학회 2017 Gut and Liver Vol.11 No.1
Background/Aims: α-Fetoprotein (AFP) is normally < 10 ng/mL in adults without malignancy or liver regeneration. However, hereditary or nonhereditary persistence of AFP in healthy adults may be encountered in clinical practice. This study describes four cases of persistent AFP elevation in healthy adults and investigates mutations in key transcription regulatory regions of the AFP gene as potential drivers of AFP overexpression. Methods: Four healthy adults with persistently elevated AFP levels (12.1 to 186.1 ng/mL) for >1 year, and 20 controls with low AFP levels (<0.61 to 2.9 ng/mL) were included in the study. AFP levels were collected from the families of two of the patients. We sequenced five regions that are critical for AFP expression: a promoter, two enhancers, and two silencers. Results: One of the two cases in which family information was represented is the first case of hereditary persistence of AFP in South Korea. Mutations related to AFP overexpression were not found in the transcription regulatory regions among the four patients. Conclusions: Persistent AFP elevation is a heterogeneous condition with or without a hereditary pattern and may be caused by factors outside of transcription regulatory region changes. Further research on the mechanism of AFP elevation is needed. (Gut Liver 2017;11:136-141)
협력중심 자기결정 교수모델을 활용한 진로교육 프로그램이 초등학교 통합학급 학생의 진로자기효능감, 자기결정력 및 전환목표달성에 미치는 영향
전민지 ( Jeon Min Ji ),이숙향 ( Lee Suk-hyang ) 한국정서행동장애학회(구 한국정서·행동장애아교육학회) 2021 정서ㆍ행동장애연구 Vol.37 No.1
본 연구에서는 협력중심 자기결정 교수모델을 활용한 진로교육 프로그램을 개발함으로써 본 프로그램이 초등학교 통합학급 학생의 진로자기효능감, 자기결정력 및 전환목표달성에 미치는 영향을 알아보고자 하였다. 또한, 초등학교 5학년과 중학교 진학을 앞두고 있는 6학년 학생들 간의 학년에 따른 차이가 있는지도 알아보고자 하였다. 본 연구를 위해 서울에 위치한 초등학교에 재학 중인 통합학급 학생 39명(비장애학생 37명, 장애학생 2명)이 참여하였고, 통합학급 실과와 창의적 체험활동 시간에 총 11회기에 걸쳐 프로그램이 진행되었다. 협력중심자기결정 교수모델을 활용한 진로교육 프로그램에 참여한 비장애학생의 진로자기효능감, 자기결정력 변화는 통계적으로 유의하지 않은 것으로 나타났으나 학년별 점수 변화의 차이는 통계적으로 유의하였다. 장애학생의 경우 진로자기효능감에서는 모두 긍정적 변화를 보였고, 자기결정력에서는 1명만 점수가 증가하였다. 또한 전환목표를 달성한 통합학급 학생 수와 목표달성 학생 수의 비율이 점차 증가하였다. 이러한 연구결과를 바탕으로 초등학교 통합학급 학생을 위한 진로교육 프로그램과 관련하여 향후 연구에 대한 논의 및 의의를 제시하였다. The purpose of this study is to examine the effects of career education programs using the Collaboration-based Instruction Model for Self-Determination (CIMSD), which was designed for inclusive elementary students on their career self-efficacy, self-determination, and transition goal-attainment along with differences in the effects across grade levels. The participants of this study were 5th and 6th grade students (a total of 39 students including 37 students without disabilities and 2 students with disabilities) of C elementary school located in Seoul. This study used a one-group pretest-posttest design. The program was implemented a total of 11 sessions by researcher. The results of this study are as follows: First, the students without disabilities in the inclusive elementary class showed a small positive change in career self-efficacy. However, this difference was not statistically significant. Analyzing the difference of score change by grade of students without disabilities showed a statistically significant positive change. The two students with disabilities showed positive change in career self-efficacy. Second, the students without disabilities in the inclusive elementary class showed a small positive change in self-determination. However, this difference was not statistically significant. Analyzing the difference of score change by grade of students without disabilities showed a statistically significant positive change. One student with disability showed positive change in self-determination, but the other student with disability showed negative change in self-determination. Third, as the program progressed, the number of students who achieved the transition goal and the ratio of students who achieved the goal in inclusive elementary class gradually increased. Based on the results, discussions and implications were provided for future studies related to effective career education programs.