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Fubao Liu,Xiao Liu,Biwu Zhu,Hui Yang,Gang Xiao,Mingyue Hu 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.6
High strain-rate rolling (≥ 10 s−1) has been successfully employed to produce AZ31 magnesium alloy sheets to a high reductionof 82% with a fine grain structure in a single pass. The rolled sheets inevitably undertake various forming processessuch as press and deep forming in the fabrication stage. To investigate the formability, the Erichsen tests were carried out onhigh strain rate rolled AZ31 magnesium alloy sheets at room temperature. The relationship among mechanical properties,microstructure and formability were investigated. The crack behavior during Erichsen tests was also studied. It was foundthat the formability was related to the lowest in-plane tensile strengthen and dislocation density of initial microstructure. The competitive relationship between micro-crack and twinning/shear bands would also influence the formability of highstrain rate rolled magnesium alloy sheets. Deformed shear band with substaintial twins prohibits the crack extension andenhances strengthen of materials in front of crack tip.
劉慧軍 한국중국문화학회 2000 中國學論叢 Vol.9 No.-
5·4운동은 중국 역사상 위대한 사상변혁운동이다. 5·4운동이 제창한 "立人"사상은 당시뿐만 아니라 현재에 있어서도 중국문화의 발전과 변화에 중요한 의의를 지니고 있다. 그러나 여러 가지 원인 때문에 5·4정신은 잊혀졌다. 본고는 정치와 문화적 측면에서 5·4정신이 잊혀지게 된 원인을 검토한 바, 다음 3가지가 주된 원인으로 작용하였다고 분석하였다. 첫째, 중국전통문화의 뿌리가 확고하여 민주사상이 단시간 내에 확립될 수 없었다. 둘째, 정치의 막강한 흡인력이 신문화운동 발전에 영향을 미쳐 지식인이 문화적 사고를 포기하여, 중국의 사상계몽이 시작되자마자 중단되었다. 셋째, 5·4운동 후 권력자의 남용이 5·4정신을 잊혀지게 하는 중요한 원인이 되었다.
Array-based Nano-amplification Technique Was Applied in Detection of Hepatitis E Virus
Liu, Hui-Hui,Cao, Xuan,Yang, Yong,Liu, Ming-Gui,Wang, Ye-Fu Korean Society for Biochemistry and Molecular Biol 2006 Journal of biochemistry and molecular biology Vol.39 No.3
A rapid method for the detection of Hepatitis E Virus (HEV) was developed by utilizing nano-gold labeled oligonucleotide probes, silver stain enhancement and the microarray technique. The 5'-end -$NH_2$ modified oligonucleotide probes were immobilized on the surface of the chip base as the capture probe. The detection probe was made of the 3'-end -SH modified oligonucleotide probe and nano-gold colloid. The optimal concentrations of these two probes were determined. To test the detection sensitivity and specificity of this technique, a conservative fragment of the virus RNA was amplified by the RT-PCR/PCR one step amplification. The cDNA was hybridized with the capture probes and the detection probes on microarray. The detection signal was amplified by silver stain enhancement and could be identified by naked eyes. 100 fM of amplicon could be detected out on the microarray. As the results, preparation of nano-gold was improved and faster. Development time also was shortened to 2 min. Thus, considering high efficiency, low cost, good specificity and high sensitivity, this technique is alternative for the detection of HEV.
Yang Hui-Hui,Jiang Hui-Ling,Tao Jia-Hao,Zhang Chen-Yu,Xiong Jian-Bing,Yang Jin-Tong,Liu Yu-Biao,Zhong Wen-Jing,Guan Xin-Xin,Duan Jia-Xi,Zhang Yan-Feng,Liu Shao-Kun,Jiang Jian-Xin,Zhou Yong,Guan Cha-Xi 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-
Necroptosis is the major cause of death in alveolar epithelial cells (AECs) during acute lung injury (ALI). Here, we report a previously unrecognized mechanism for necroptosis. We found an accumulation of mitochondrial citrate (citratemt) in lipopolysaccharide (LPS)-treated AECs because of the downregulation of Idh3α and citrate carrier (CIC, also known as Slc25a1). shRNA- or inhibitor–mediated inhibition of Idh3α and Slc25a1 induced citratemt accumulation and necroptosis in vitro. Mice with AEC-specific Idh3α and Slc25a1 deficiency exhibited exacerbated lung injury and AEC necroptosis. Interestingly, the overexpression of Idh3α and Slc25a1 decreased citratemt levels and rescued AECs from necroptosis. Mechanistically, citratemt accumulation induced mitochondrial fission and excessive mitophagy in AECs. Furthermore, citratemt directly interacted with FUN14 domain-containing protein 1 (FUNDC1) and promoted the interaction of FUNDC1 with dynamin-related protein 1 (DRP1), leading to excessive mitophagy-mediated necroptosis and thereby initiating and promoting ALI. Importantly, necroptosis induced by citratemt accumulation was inhibited in FUNDC1-knockout AECs. We show that citratemt accumulation is a novel target for protection against ALI involving necroptosis.
Jun-Jie LIU,Liu-Yang LU,Hui-Hui LIU,Ya-She LI,Xu SU,Bai-Zhong ZHANG,Xi-Ling CHEN 한국곤충학회 2019 Entomological Research Vol.49 No.8
The greenbug, Schizaphis graminum Rondani is a major pest species of wheat crops. In this study, a transcriptome sequencing, and the expression of the 12 genes related to insecticide resistance were conducted in S. graminum. The sequencing and subsequent bioinformatics analysis outputed 46,593 unigenes, among which 28,289 unigenes were annotated to corresponding functions by blasting with high homologous genes in database, giving annotation rate of 60.72%. To gain insight into the mechanism of insecticide resistance, the expression of the 12 genes related to insecticide resistance for S. graminum was investigated. The expression level of aminopeptidase N (AN), cytochrome P450 (CYP), acetylcholinesterase 1 (AC), catalase (CAT), cytochrome c oxidas (CCC), GABA receptor (GABA), glutathione S-transferase (GST) were highest in the apterous nymphs among different developmental stages; The expression level of AN, CBL, CYP, CA, SD, and GST were relatively more abundant in the abdomen compared to head and throax. The results could give out the key information about the relationship between the expression of these genes in different developmental stages, tissues, treatments and metabolism of insecticides. These genes that were co-up-regulated significantly in third instar nymphs of S. graminum induced by imidacloprid, were consistent with their putative involvement in insecticide resistance. This provides most comprehensive transcriptome data for S. graminum to further study and managenment.
Liu, S.,Hui, K.S.,Hui, K.N.,Jadhav, V.V.,Xia, Q.X.,Yun, J.M.,Cho, Y.R.,Mane, R.S.,Kim, K.H. Pergamon Press 2016 ELECTROCHIMICA ACTA Vol.188 No.-
Porous microspheres copper cobalt carbonate hydroxides (Cu<SUB>x</SUB>Co<SUB>2@?x</SUB>CH) pseudocapacitive electrode material comprised of nanoplates via a facile hydrothermal method were presented. Significantly, the crystalline structure, morphology and electrochemical performance of the Cu<SUB>x</SUB>Co<SUB>2@?x</SUB>CH can be readily manipulated by varying the Cu/Co molar ratios. Among various stoichiometries of Cu<SUB>x</SUB>Co<SUB>2@?x</SUB>CH porous microspheres studied, Cu<SUB>1.79</SUB>Co<SUB>0.21</SUB>CH consisted of nanoplates with a mean thickness of @?35nm showed a high specific capacitance of 789Fg<SUP>@?1</SUP> at a current destiny of 1Ag<SUP>@?1</SUP> and good rate ability. Furthermore, the optimized Cu<SUB>1.79</SUB>Co<SUB>0.21</SUB>CH electrode also exhibited remarkable high cycling stability, ca 77.5% after 3000 charge/discharge cycles at current density of 5Ag<SUP>@?1</SUP>. An asymmetric device was constructed from optimized Cu<SUB>1.79</SUB>Co<SUB>0.21</SUB>CH materials on nickel foam (NF) as cathode electrode and graphene on NF as anode electrode in a 6M KOH electrolyte. The asymmetric Cu<SUB>1.79</SUB>Co<SUB>0.21</SUB>CH/NF//graphene/NF supercapacitor device delivered a specific capacitance of 60.5Fg<SUP>@?1</SUP> in a potential range of 0@?1.6V. A high energy density of 21.5Whkg<SUP>@?1</SUP> was achieved at the power density of 200Wkg<SUP>@?1</SUP>. More significantly, the designed device exhibited excellent cycling stability with 73.3% capacity retention after 5000 cycles.
Liu, Hui,Wang, Sixin,Zhang, Dongyan,Wang, Jing,Zhang, Wei,Wang, Yamin,Ji, Haifeng Asian Australasian Association of Animal Productio 2020 Animal Bioscience Vol.33 No.1
Objective: This study was conducted to determine the effects of dietary supplementation with Pediococcus acidilactici (P. acidilactici) ZPA017 as a probiotic on reproductive performance, fecal microbial flora and serum indices in sows during late gestation and lactation. Methods: A total of 94 sows (Large White×Yorkshire, average 4.50 parities) were randomly allotted to two dietary treatments: control diet and the diet supplemented with P. acidilactici ZPA017 (2.40×10<sup>9</sup> colony-forming unit/kg of diets). The study started at d 90 of gestation and conducted until d 28 of lactation. Results: Compared to sows fed the control diet, supplementation of P. acidilactici ZPA017 increased the number of weaning piglets, weaning weight of litter and piglets, survival rate of piglets at weaning (p<0.05), and decreased diarrhea rate of piglets in lactation (p<0.05). Dietary P. acidilactici ZPA017 increased fecal Lactobacillus populations (p = 0.030) and reduced fecal Escherichia coli and Staphylococcus aureus populations (p<0.05) of sows at weaning. Moreover, the supplementation of P. acidilactici ZPA017 increased serum concentrations of immunoglobulin G, immunoglobulin A and total protein (p<0.05), while decreased serum haptoglobin concentration and alanine aminotransferase activity (p<0.05) of sows at weaning. Conclusion: Administration of P. acidilactici ZPA017 in diets during late gestation and lactation had positive effects on the reproductive performance, intestinal microflora balance and immunity of sows.
Liu, Shude,Hui, Kwan San,Hui, Kwun Nam,Li, Hai-Feng,Ng, Kar Wei,Xu, Jincheng,Tang, Zikang,Jun, Seong Chan The Royal Society of Chemistry 2017 Journal of Materials Chemistry A Vol.5 No.36
<▼1><P>Cycling stability of an asymmetric supercapacitor is enhanced using hierarchical porous NiGa2O4 nanosheets as cathode electrode.</P></▼1><▼2><P>Rational design of the composition and electrochemically favorable structural configuration of electrode materials are highly required to develop high-performance supercapacitors. Here, we report our findings on the design of interconnected NiGa2O4 nanosheets as advanced cathode electrodes for supercapacitors. Rietveld refinement analysis demonstrates that the incorporation of Ga into NiO leads to a larger cubic lattice parameter that promotes faster charge-transfer kinetics, enabling significantly improved electrochemical performance. The NiGa2O4 electrode delivers a specific capacitance of 1508 F g<SUP>−1</SUP> at a current density of 1 A g<SUP>−1</SUP> with a capacitance retention of 63.7% at 20 A g<SUP>−1</SUP>, together with excellent cycling stability after 10 000 charge–discharge cycles (capacitance retention of 102.4%). An asymmetric supercapacitor device was assembled by using NiGa2O4 and Fe2O3 as cathode and anode electrodes, respectively. The ASC delivers a high energy density of 45.2 W h kg<SUP>−1</SUP> at a power density of 1600 W kg<SUP>−1</SUP> with exceptional cycling stability (94.3% cell capacitance retention after 10 000 cycles). These results suggest that NiGa2O4 can serve as a new class cathode material for advanced electrochemical energy storage applications.</P></▼2>