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Fubao Liu,Xiao Liu,Biwu Zhu,Hui Yang,Gang Xiao,Mingyue Hu 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.6
High strain-rate rolling (≥ 10 s−1) has been successfully employed to produce AZ31 magnesium alloy sheets to a high reductionof 82% with a fine grain structure in a single pass. The rolled sheets inevitably undertake various forming processessuch as press and deep forming in the fabrication stage. To investigate the formability, the Erichsen tests were carried out onhigh strain rate rolled AZ31 magnesium alloy sheets at room temperature. The relationship among mechanical properties,microstructure and formability were investigated. The crack behavior during Erichsen tests was also studied. It was foundthat the formability was related to the lowest in-plane tensile strengthen and dislocation density of initial microstructure. The competitive relationship between micro-crack and twinning/shear bands would also influence the formability of highstrain rate rolled magnesium alloy sheets. Deformed shear band with substaintial twins prohibits the crack extension andenhances strengthen of materials in front of crack tip.
劉慧軍 한국중국문화학회 2000 中國學論叢 Vol.9 No.-
5·4운동은 중국 역사상 위대한 사상변혁운동이다. 5·4운동이 제창한 "立人"사상은 당시뿐만 아니라 현재에 있어서도 중국문화의 발전과 변화에 중요한 의의를 지니고 있다. 그러나 여러 가지 원인 때문에 5·4정신은 잊혀졌다. 본고는 정치와 문화적 측면에서 5·4정신이 잊혀지게 된 원인을 검토한 바, 다음 3가지가 주된 원인으로 작용하였다고 분석하였다. 첫째, 중국전통문화의 뿌리가 확고하여 민주사상이 단시간 내에 확립될 수 없었다. 둘째, 정치의 막강한 흡인력이 신문화운동 발전에 영향을 미쳐 지식인이 문화적 사고를 포기하여, 중국의 사상계몽이 시작되자마자 중단되었다. 셋째, 5·4운동 후 권력자의 남용이 5·4정신을 잊혀지게 하는 중요한 원인이 되었다.
Array-based Nano-amplification Technique Was Applied in Detection of Hepatitis E Virus
Liu, Hui-Hui,Cao, Xuan,Yang, Yong,Liu, Ming-Gui,Wang, Ye-Fu Korean Society for Biochemistry and Molecular Biol 2006 Journal of biochemistry and molecular biology Vol.39 No.3
A rapid method for the detection of Hepatitis E Virus (HEV) was developed by utilizing nano-gold labeled oligonucleotide probes, silver stain enhancement and the microarray technique. The 5'-end -$NH_2$ modified oligonucleotide probes were immobilized on the surface of the chip base as the capture probe. The detection probe was made of the 3'-end -SH modified oligonucleotide probe and nano-gold colloid. The optimal concentrations of these two probes were determined. To test the detection sensitivity and specificity of this technique, a conservative fragment of the virus RNA was amplified by the RT-PCR/PCR one step amplification. The cDNA was hybridized with the capture probes and the detection probes on microarray. The detection signal was amplified by silver stain enhancement and could be identified by naked eyes. 100 fM of amplicon could be detected out on the microarray. As the results, preparation of nano-gold was improved and faster. Development time also was shortened to 2 min. Thus, considering high efficiency, low cost, good specificity and high sensitivity, this technique is alternative for the detection of HEV.
Yang Hui-Hui,Jiang Hui-Ling,Tao Jia-Hao,Zhang Chen-Yu,Xiong Jian-Bing,Yang Jin-Tong,Liu Yu-Biao,Zhong Wen-Jing,Guan Xin-Xin,Duan Jia-Xi,Zhang Yan-Feng,Liu Shao-Kun,Jiang Jian-Xin,Zhou Yong,Guan Cha-Xi 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-
Necroptosis is the major cause of death in alveolar epithelial cells (AECs) during acute lung injury (ALI). Here, we report a previously unrecognized mechanism for necroptosis. We found an accumulation of mitochondrial citrate (citratemt) in lipopolysaccharide (LPS)-treated AECs because of the downregulation of Idh3α and citrate carrier (CIC, also known as Slc25a1). shRNA- or inhibitor–mediated inhibition of Idh3α and Slc25a1 induced citratemt accumulation and necroptosis in vitro. Mice with AEC-specific Idh3α and Slc25a1 deficiency exhibited exacerbated lung injury and AEC necroptosis. Interestingly, the overexpression of Idh3α and Slc25a1 decreased citratemt levels and rescued AECs from necroptosis. Mechanistically, citratemt accumulation induced mitochondrial fission and excessive mitophagy in AECs. Furthermore, citratemt directly interacted with FUN14 domain-containing protein 1 (FUNDC1) and promoted the interaction of FUNDC1 with dynamin-related protein 1 (DRP1), leading to excessive mitophagy-mediated necroptosis and thereby initiating and promoting ALI. Importantly, necroptosis induced by citratemt accumulation was inhibited in FUNDC1-knockout AECs. We show that citratemt accumulation is a novel target for protection against ALI involving necroptosis.
Jun-Jie LIU,Liu-Yang LU,Hui-Hui LIU,Ya-She LI,Xu SU,Bai-Zhong ZHANG,Xi-Ling CHEN 한국곤충학회 2019 Entomological Research Vol.49 No.8
The greenbug, Schizaphis graminum Rondani is a major pest species of wheat crops. In this study, a transcriptome sequencing, and the expression of the 12 genes related to insecticide resistance were conducted in S. graminum. The sequencing and subsequent bioinformatics analysis outputed 46,593 unigenes, among which 28,289 unigenes were annotated to corresponding functions by blasting with high homologous genes in database, giving annotation rate of 60.72%. To gain insight into the mechanism of insecticide resistance, the expression of the 12 genes related to insecticide resistance for S. graminum was investigated. The expression level of aminopeptidase N (AN), cytochrome P450 (CYP), acetylcholinesterase 1 (AC), catalase (CAT), cytochrome c oxidas (CCC), GABA receptor (GABA), glutathione S-transferase (GST) were highest in the apterous nymphs among different developmental stages; The expression level of AN, CBL, CYP, CA, SD, and GST were relatively more abundant in the abdomen compared to head and throax. The results could give out the key information about the relationship between the expression of these genes in different developmental stages, tissues, treatments and metabolism of insecticides. These genes that were co-up-regulated significantly in third instar nymphs of S. graminum induced by imidacloprid, were consistent with their putative involvement in insecticide resistance. This provides most comprehensive transcriptome data for S. graminum to further study and managenment.
Hui Liu,Yongxia Wei,Long Wang,Xian Wu 보안공학연구지원센터 2015 International Journal of u- and e- Service, Scienc Vol.8 No.3
Soil erosion is one of the most widespread ecological problems with the most serious damage worldwide. In the present study, the influence of soil erosion thickness on soybean yield of black soil in Northeast China is studied. With the pot experiment as the method, this study investigates the influence by peeling the surface black soil layer at 0, 5, 10, 20, 25, and 30 cm to simulate different soil erosion thicknesses, establishes a quantitative model of soybean yield to explore the response of soybean yield to soil erosion thickness. The 4-D-optimum orthogonal design (416-A) is adopted to conduct the coupling experiment of water and fertilizers under erosion thicknesses of 0 and 15 cm so that the coupling modes of water and fertilizers under the two soil erosion thicknesses are established. The results show that soil erosion thickness has a significant influence on soybean growth, that is, with the increase in the soil erosion thickness, soybean plant height, pods and grains per plant all reduce, and soybean yield decreases in a Z-shaped curve, while yield reduction rate increases in a S-shaped curve, respectively. Water, along with N, P, and K fertilizers, affect soybean yield, but the intensity varies with soil erosion thickness. Under the soil erosion thickness of 0 cm, the order is: W>P>N>K. While under the soil erosion thickness of 15 cm, the order is: N>W>P>K. With the increase in soil erosion thickness and the decrease in soil fertility, the effect of irrigation reduces, whereas that of fertilization increases. The research results can provide the theoretical basis for soil erosion prevention and control in the black soil region.
Liu, Shude,Yin, Ying,San Hui, Kwan,Hui, Kwun Nam,Lee, Su Chan,Chan Jun, Seong Elsevier 2019 ELECTROCHIMICA ACTA Vol.297 No.-
<P><B>Abstract</B></P> <P>Rational design of electrode structures has been recognized as an effective strategy to improve the electrochemical performance of electrode materials. Herein, we demonstrate an integrated electrode in which nickel hydroxide (Ni(OH)<SUB>2</SUB>) nanosheets are deposited on both sides of chemical vapor deposition-grown graphene on Ni foam, which not only effectively optimizes electrical conductivity of Ni(OH)<SUB>2</SUB>, but also accommodates the structural deformation associated with the large volume change upon cycling. The synthesized Ni(OH)<SUB>2</SUB>/graphene/Ni(OH)<SUB>2</SUB>/Ni foam electrode exhibits a high specific capacity of 991 C g<SUP>−1</SUP> at a current density of 1 A g<SUP>−1</SUP>, which is higher than the theoretical specific capacity of additive sum of Ni(OH)<SUB>2</SUB> and graphene, and retains 95.4% of the initial capacity after 5000 cycles. A hybrid supercapacitor is constructed by using Ni(OH)<SUB>2</SUB>/graphene/Ni(OH)<SUB>2</SUB>/Ni foam as the positive electrode and activated carbon on Ni foam as the negative electrode, which achieves a maximum energy density of 49.5 W h kg<SUP>−1</SUP> at a power density of 750 W kg<SUP>−1</SUP>, and excellent cycling lifespans with 89.3% retention after 10000 cycles at 10 A g<SUP>−1</SUP>.</P>
Study on Importance Function for Particle Filter
Liu Lu,Meng Yang,Shu Geng,Shu-fen Wang,Yong-hui Wang 보안공학연구지원센터 2015 International Journal of Multimedia and Ubiquitous Vol.10 No.2
As an important nonlinear filter theory, particle filter is a heated issue in domestic and foreign researches. The option of importance density is one of the key steps of particle filter algorithm. The proper option of importance density can minish the negative influence of filter algorithm caused by degeneracy problem. This paper introduces several widely-used options of importance density systemically, and analyzes their features and applied perspectives respectively. The paper also advances a comprehensive method of importance density, analyzes its technical features, explores the adjudgement and improvement of this method based on various performance, and finally puts forward the necessary further study according to the engineer requirements.