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Mingle Wang,Jing Zhuang,Zhongwei Zou,Qinghui Li,Huahong Xin,Xinghui Li 한국식물학회 2017 Journal of Plant Biology Vol.60 No.5
Dehydration-responsive element-binding protein(DREB) transcription factors play key roles in plant stresssignal transduction pathways. We herein describe the functionsof a Camellia sinensis DREB transcription factor (CsDREB)in response to abiotic stress. Subcellular localization analysesindicated that the CsDREB localizes to the nucleus. CsDREBexpression in C. sinensis leaves was induced by heat, cold,drought, high salinity, H2O2, and exogenous abscisic acid(ABA). Additionally, CsDREB showed no transcriptionalactivation in Saccharomyces cerevisiae. Transgenic Arabidopsisthaliana plants overexpressing CsDREB exhibited enhancedtolerance to salt and drought stresses. The overexpression ofCsDREB in A. thaliana plants resulted in the up-regulatedexpression of ABA-dependent stress-induced genes (i.e.,AtRD29B, AtRAB18, AtABI1, and AtABI2) and ABAindependentstress-induced genes (i.e., AtCOR15a andAtRD29A). Furthermore, an analysis of the CsDREB promotersequence revealed the presence of several abiotic and bioticstress-related motifs, along with the developmental stageandtissue-specific elements. An examination of thetransient expression of the CsDREB promoter in Nicotianabenthamiana leaves revealed that the promoter is highlyresponsive to ABA and methyl jasmonate. Collectively, theseresults suggest that CsDREB may increase plant tolerance tosalt and drought stresses via both ABA-dependent and ABAindependentpathways.
( Kok-ann Gwee ),( Paul Bergmans ),( Jinyong Kim ),( Bogdana Coudsy ),( Angelia Sim ),( Minhu Chen ),( Lin Lin ),( Xiaohua Hou ),( Huahong Wang ),( Khean-lee Goh ),( John A Pangilinan ),( Nayoung Kim 대한소화기기능성질환·운동학회(구 대한소화관운동학회) 2017 Journal of Neurogastroenterology and Motility (JNM Vol.23 No.2
Background/Aims There is a need for a simple and practical tool adapted for the diagnosis of chronic constipation (CC) in the Asian population. This study compared the Asian Neurogastroenterology and Motility Association (ANMA) CC tool and Rome III criteria for the diagnosis of CC in Asian subjects. Methods This multicenter, cross-sectional study included subjects presenting at outpatient gastrointestinal clinics across Asia. Subjects with CC alert symptoms completed a combination Diagnosis Questionnaire to obtain a diagnosis based on 4 different diagnostic methods: self-defined, investigator`s judgment, ANMA CC tool, and Rome III criteria. The primary endpoint was the level of agreement/ disagreement between the ANMA CC diagnostic tool and Rome III criteria for the diagnosis of CC. Results The primary analysis comprised of 449 subjects, 414 of whom had a positive diagnosis according to the ANMA CC tool. Rome III positive/ANMA positive and Rome III negative/ANMA negative diagnoses were reported in 76.8% and 7.8% of subjects, respectively, resulting in an overall percentage agreement of 84.6% between the 2 diagnostic methods. The overall percentage disagreement between these 2 diagnostic methods was 15.4%. A higher level of agreement was seen between the ANMA CC tool and self-defined (374 subjects [90.3%]) or investigator`s judgment criteria (388 subjects [93.7%]) compared with the Rome III criteria. Conclusion This study demonstrates that the ANMA CC tool can be a useful for Asian patients with CC. (J Neurogastroenterol Motil 2017;23:262-272)