Study on the Carbohydrate and Isozyme Changes during Fresh Tap Root Formation and Thickening in Radish (Raphanus sativus L)

Wei Wang,Zange Jing,Yan Wang,Yiqin Gong,Liwang Liu 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

Nuciferine modulates the gut microbiota and prevents obesity in high-fat diet-fed rats

Wang Yueping,Yao Weifan,Li Bo,Qian Shiyun,Wei Binbin,Gong Shiqiang,Wang Jing,Liu Mingyan,Wei Minjie 생화학분자생물학회 2020 Experimental and molecular medicine Vol.52 No.-

Gut microbiota dysbiosis has a significant role in the pathogenesis of metabolic diseases, including obesity. Nuciferine (NUC) is a main bioactive component in the lotus leaf that has been used as food in China since ancient times. Here, we examined whether the anti-obesity effects of NUC are related to modulations in the gut microbiota. Using an obese rat model fed a HFD for 8 weeks, we show that NUC supplementation of HFD rats prevents weight gain, reduces fat accumulation, and ameliorates lipid metabolic disorders. Furthermore, 16S rRNA gene sequencing of the fecal microbiota suggested that NUC changed the diversity and composition of the gut microbiota in HFD-fed rats. In particular, NUC decreased the ratio of the phyla Firmicutes/Bacteroidetes, the relative abundance of the LPS-producing genus Desulfovibrio and bacteria involved in lipid metabolism, whereas it increased the relative abundance of SCFA-producing bacteria in HFD-fed rats. Predicted functional analysis of microbial communities showed that NUC modified genes involved in LPS biosynthesis and lipid metabolism. In addition, serum metabolomics analysis revealed that NUC effectively improved HFD-induced disorders of endogenous metabolism, especially lipid metabolism. Notably, NUC promoted SCFA production and enhanced intestinal integrity, leading to lower blood endotoxemia to reduce inflammation in HFD-fed rats. Together, the anti-obesity effects of NUC may be related to modulations in the composition and potential function of gut microbiota, improvement in intestinal barrier integrity and prevention of chronic low-grade inflammation. This research may provide support for the application of NUC in the prevention and treatment of obesity.

Short-Term Forecasting for Harbor Waterway Currents Speeds

Cheng Gong,Yan Lv,Chunjiang Zhang,Xiyuan Wang,Wei Huangfu,Zhongshan Zhang 보안공학연구지원센터 2014 International Journal of Multimedia and Ubiquitous Vol.9 No.12

The ocean currents speeds in the harbor waterway are directly related to the ability of the ship to in or out the harbor. Accurately predict the speeds can assist the ship to choose the right time for sailing. To solve this problem, we chose two models of linear and non-linear prediction. We had set sensors in Qinhuangdao for a long time, then using the collected data for training. Our test is using a lot of random data to train and predict with different steps and orders. The results show that both methods can use less original data to train the model, and finally achieve preferably prediction. According to the characteristics of Qinhuangdao harbor, Auto-Regressive (AR) model is more appropriate than Support Vector Regression (SVR) model.

Abnormal Seedlings Emerged during Embryo Rescue and Its Remedy for Seedless Grape Breeding

Wei Ji,Zhiqian Li,Wenkong Yao,Peijie Gong,Yuejin Wang 한국원예학회 2013 원예과학기술지 Vol.31 No.4

The abnormal seedlings, a common physiological anomalies, emerged during embryo rescue severely restricted grape breeding. To enhance the efficiency of the seedless grape breeding by reducing the production of abnormal seedlings in the course of embryo rescue, we investigated the effects of genotype, media type, embryo style, pre-chilling on the deformity rate of the abnormal seedlings during embryo rescue. The abnormal seedlings were firstly classified into seven categories based on their morphology. Our results indicated that the emergence of abnormal seedlings was highly dependent on the female parent genotype. Polyembryony was advantageous to diminish the number of abnormal plantlets and the germination rate of embryo was 100%. We also found that pre-chilling treatment could reduce the number of abnormal plantlets and promote the embryo germination. The abnormal plantlets were reduced significantly by the addition of ZnSO₄ 10 μmol・L<SUP>-1</SUP> or mashed-banana 500 mg・L<SUP>-1</SUP> to either embryo development or germination media. Transferring the abnormal seedlings onto the suitable fresh media in 4 weeks after embryo germination provided an effective way to transform them into normal seedlings.

Paleostress reconstructions and stress regimes in the Nanchuan region of Sichuan Basin, South China: implications for hydrocarbon exploration

Wei Ju,Jilin Wang,Huihuang Fang,Yunpeng Gong,Shanjin Zhang 한국지질과학협의회 2017 Geosciences Journal Vol.21 No.4

The Longmaxi shales are the major hydrocarbon source rocks for Sichuan Basin and adjacent regions in China. Tectonic stress field is important in controling the migration and accumulation of shale gas. In the present study, natural fractures in the Nanchuan region of Sichuan Basin were extensively studied and analyzed with the Rotational Optimization Method (ROM) to reconstruct paleostress fields and their transformation processes. The dominant strikes of natural fractures were in the NW-SE, NESW, NWW-SEE and NEE-SWW directions with high-angle oblique or vertical (70~90°) dip angles. Paleostress reconstructions indicated that the Late Yanshanian and Late Himalayan stress fields controlled the tectonics in the Nanchuan region. The orientation of the maximum principal stress (σ1) during the Late Yanshanian and Late Himalayan period was ~NW-SE and ~NE-SW trending, respectively. In addition, the majority of stress regimes during the two tectonic stages were strike-slip regimes (SS). Remote effects of the westward subduction of the paleo-Pacific plate activated the Xuefengshan tectonic zone and generated the Late Yanshanian paleostress field. Later, the change in the stress field was due to the variation of tectonic activity intensity in the Xuefengshan and Dabashan tectonic zones. The paleotectonic stress fields and their transformation processes comprehensively influenced the migration and accumulation of hydrocarbons in the Nanchuan region.

Efficient Synthesis of Functionalized 1-oxo-1-phenyl-2-acetic Acids through Ru(II)-catalyzed Transfer Hydrogenation

Wang, Xiaowei,Yan, Yunnan,Gong, Binwei,Tang, Xiaobo,Li, Qiu,Meng, Yanqiu,Xu, H. Eric,Yi, Wei Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.10

Adenovirus-mediated Expression of Both Antisense Ornithine Decarboxylase and S-adenosylmethionine Decarboxylase Induces G₁ Arrest in HT-29 Cells

Gong, Lei,Jiang, Chunying,Zhang, Bing,Hu, Haiyan,Wang, Wei,Liu, Xianxi Korean Society for Biochemistry and Molecular Biol 2006 Journal of biochemistry and molecular biology Vol.39 No.6

To evaluated the effect of recombinant adenovirus Ad-ODC-AdoMetDCas which can simultaneously express both antisense ornithine decarboxylase (ODC) and S-adenosylmethionine decarboxylase (AdoMetDC) on cell cycle distribution in colorectal cancer cell and investigated underlying regulatory responses, human colorectal cancer cells HT-29 were cultured in RPMI 1640 medium and infected with Ad-ODC-AdoMetDCas. Cell cycle progression was detected by flow cytometry analysis. The expression levels of cell cycle regulated proteins were measured by Western blot analysis. The mRNA level of cyclin D1 was measured by RT-PCR. And a luciferase reporter plasmid of cyclin D1 promoter was constructed to observe the effect of Ad-ODC-AdoMetDCas on cyclin D1 promoter activity. The results showed that recombinant adenovirus Ad-ODC-AdoMetDCas significantly induced $G_1$ arrest, decreased levels of cyclin D1 protein and mRNA and suppressed the promoter activity. Ad-ODC-AdoMetDCas also inhibited nuclear translocation of $\beta$-catenin. In conclusion, downregulation of ODC and AdoMetDC mediated by Ad-ODC-AdoMetDCas transfection induces $G_1$ arrest in HT-29 cells and the arrest was associated with suppression of cyclin D1 expression and inhibition of $\beta$-catenin nuclear translocation. As a new anticancer reagent, the recombinant adenovirus Ad-ODC-AdoMetDCas holds promising hope for the therapy of colorectal cancers.

Inequalities in Awareness and Attitude towards HPV and Its Vaccine between Local and Migrant Residents Who Participated in Cervical Cancer Screening in Shenzhen, China

Wei Lin,Yueyun Wang,Zhihua Liu,Bin Chen,Shixin Yuan,Bo Wu,Lin Gong 대한암학회 2020 Cancer Research and Treatment Vol.52 No.1

Purpose A cross-sectional survey was conducted to evaluate the differences on awareness and attitude towards human papillomavirus (HPV) and its vaccine between local and migrant residents who participated in cervical cancer screening in Shenzhen, China. Materials and Methods A total of 9,855 females sampled from healthcare institutions in 20 street blocks through the Cervical Cancer Prevention Network were surveyed in this study by a self-administered questionnaire. Multivariate logistic regression was conducted to explore the role of the hukou and resident status in the willingness to receive HPV vaccination. Results Local residents had a relatively higher awareness of HPV (62.0% vs. 35.6% vs. 29.9%, p < 0.001) and its vaccine (35.3% vs. 15.4% vs. 14.8%, p < 0.001), as well as a higher willingness to receive HPV vaccination (68.5% vs. 62.5% vs. 56.2%, p < 0.001) than non-permanent residents and floating population. Except for age, education level, marital status, monthly income, having daughter(s), and heard of HPV and its vaccine, the hukou and resident status significantly associated with the willingness to receive HPV vaccination (local residents vs. floating population: odds ratio, 1.216; 95% confidence interval, 1.057 to 1.398). None significant difference on the associated factors was found between local residents and internal migrants (p for interactions > 0.05). Conclusion Inequalities in awareness and attitude towards HPV and its vaccine existed between local and migrant residents in Shenzhen. The hukou and resident status did impact on the willingness to receive HPV vaccination, therefore, it is critical to implement effective health education campaigns on HPV and its vaccine among internal migrants.

Evaluating the monthly and interannual variation of net primary production in response to climate in Wuhan during 2001 to 2010

Wei Gong,Lunche Wang,Aiwen Lin,Miao Zhang 한국지질과학협의회 2012 Geosciences Journal Vol.16 No.3

As the difference between photosynthesis, or gross primary productivity (GPP), and autotrophic respiration (RA), net primary productivity (NPP) is a key component of the terrestrial carbon cycle. The temporal and spatial response of NPP to climate change is thus one of the most important aspects in the study of climate-vegetation relationship. In this study, we developed a new method to estimate NPP accurately by finding a linear relationship between solar radiation and photosynthetically active radiation (PAR) and improving maximum light use efficiency (LUE) of vegetation, which could be adopted and used in other regions of the world. We utilize normalized difference vegetation index (NDVI) datasets of Moderate Resolution Imaging Spectroradiometer (MODIS) from 2001 to 2010 and geographic information system (GIS) techniques to reveal the monthly and interannual change of NPP in Wuhan, China. We also applied the lagged cross-correlation analysis method to study the delayed and continuous effects on monthly and interannual variations of NPP to climatic factors (air temperature, precipitation, total radiation and sunshine percentage). The result showed that precipitation and total radiation were the major climatic factors influencing monthly variation of NPP, and sunshine percentage mostly determined the interannual variation of NPP for different vegetation. Monthly NPP showed significant positive correlation with total radiation of that month, and the effect could persist for one month; significant positive one month lagged correlation was also observed between monthly variation of NPP and precipitation, and the influences of changing climate on NPP would last for two months

Overproduction of Glutathione by L-Cysteine Addition and a Temperature-shift Strategy

Gong-Yuan Wei,Da-Hui Wang,Jian Chen 한국생물공학회 2008 Biotechnology and Bioprocess Engineering Vol.13 No.3

The present study investigated the effects of three constituent amino acids on glutathione production in flask culture of Candida utilis. Although L-glutamic acid and glycine had little impact on cell growth and glutathione biosynthesis, L-cysteine positively influenced glutathione production, despite inhibiting cell growth when it was added prior to stationary phase. Adding 8 mmol/L of L-cysteine to the culture broth at 16 h boosted glutathione production by 91%, increasing the intracellular glutathione content by 106% compared to untreated controls. A temperature-shift strategy, in which we shifted batch and fed-batch cultures of C. utilis from 30 to 26℃, also significantly enhanced glutathione production. Applying both strategies (i.e. adding 20 mmol/L L-cysteine and shifting the temperature from 30 to 26℃) at 33 h enhanced the glutathione concentration and the intracellular glutathione content to 1,312 mg/L and 3.75%, respectively, during fed-batch cultivation (glucose feeding at a constant rate of 18.3 g/h). The average specific glutathione production rate under this condition was 129% higher than that of the control without strategy.