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Lee Young Jeong,Kang Hyun Tag,Choi Ji Ho,Moon Ji Eun,Lee Youngjun,Ha Tae Kyung,Lee Ho Dong 대한수면학회 2021 sleep medicine research Vol.12 No.2
Background and Objective Few clinical studies have investigated the accuracy of non-contact monitoring devices for vital signs during sleep and sleep architecture in adults with sleep-disordered breathing (SDB). The purpose of this study was to assess the accuracy of a contactless monitoring device for 1) heart rate, respiratory rate, and body temperature during sleep and 2) sleep architecture in adults with SDB.Methods Thirty-five consecutive adults, who visited a tertiary university hospital due to suspected SDB, underwent a complete physical examination and standard (level 1) polysomnography plus body temperature measurement with a contactless monitoring device (HoneyCube System).Results A total of 30 subjects (mean age = 46.43 ± 12.9 years; male: female = 22: 8) were finally included, and five subjects were excluded due to inadequate data in this study. The intraclass correlation coefficient values of heart rate, respiratory rate, and body temperature measured using the contactless monitoring device were 0.91 (95% confidence interval [CI]: 0.892, 0.928), 0.937 (95% CI: 0.919, 0.954), and 0.918 (95% CI: 0.895, 0.941), respectively. The mean kappa value for sleep architecture was 0.562 (95% CI: 0.529, 0.596).Conclusions The contactless monitoring device showed good (almost perfect) agreement in terms of heart rate, respiratory rate, and body temperature and moderate agreement in sleep architecture with contact measurements. These results suggest that the HoneyCube System is a good candidate device for sleep monitoring at home and in multiple accommodations.
Matrix Metalloproteinase-8 Plays a Pivotal Role in Neuroinflammation by Modulating TNF-α Activation
Lee, Eun-Jung,Han, Jeong Eun,Woo, Moon-Sook,Shin, Jin A.,Park, Eun-Mi,Kang, Jihee Lee,Moon, Pyong Gon,Baek, Moon-Chang,Son, Woo-Sung,Ko, Young Tag,Choi, Ji Woong,Kim, Hee-Sun The American Association of Immunologists, Inc. 2014 JOURNAL OF IMMUNOLOGY Vol.193 No.5
<P>Matrix metalloproteinases (MMPs) play important roles in normal brain development and synaptic plasticity, although aberrant expression of MMPs leads to brain damage, including blood–brain barrier disruption, inflammation, demyelination, and neuronal cell death. In this article, we report that MMP-8 is upregulated in LPS-stimulated BV2 microglial cells and primary cultured microglia, and treatment of MMP-8 inhibitor (M8I) or MMP-8 short hairpin RNA suppresses proinflammatory molecules, particularly TNF-α secretion. Subsequent experiments showed that MMP-8 exhibits TNF-α–converting enzyme (TACE) activity by cleaving the prodomain of TNF-α (A<SUP>74</SUP>/Q<SUP>75</SUP>, A<SUP>76</SUP>/V<SUP>77</SUP> residues) and, furthermore, that M8I inhibits TACE activity more efficiently than TAPI-0, a general TACE inhibitor. Biochemical analysis of the underlying anti-inflammatory mechanisms of M8I revealed that it inhibits MAPK phosphorylation, NF-κB/AP-1 activity, and reactive oxygen species production. Further support for the proinflammatory role of microglial MMP-8 was obtained from an in vivo animal model of neuroinflammatory disorder. MMP-8 is upregulated in septic conditions, particularly in microglia. Administration of M8I or MMP-8 short hairpin RNA significantly inhibits microglial activation and expression/secretion of TNF-α in brain tissue, serum, and cerebrospinal fluid of LPS-induced septic mice. These results demonstrate that MMP-8 critically mediates microglial activation by modulating TNF-α activity, which may explain neuroinflammation in septic mouse brain.</P>
Detection of Allexiviruses in the Garlic Plants in Korea
Lee, Eun-Tag,Koo, Bong-Jin,Jung, Ji-Hue,Chang, Moo-Ung,Kang, Sang-Gu The Korean Society of Plant Pathology 2007 Plant Pathology Journal Vol.23 No.4
The genomes of different allexiviruses were isolated and cloned from virus-infected garlic plants (Allium sativum), which were collected from farm fields in the southern provinces in Korea. The partial nucleotide sequences of the genomes from different allexiviruses were clearly identified in the virus-infected garlic plants. The cloned partial genomes of viruses in garlic plants showed a greater than 90% homology to previously identified allexiviruses and classified into species of GarV-A, -B, -C, -D, -E, and -X, demonstrating that species of allexivirus found in the other countries in the world are also widely distributed in the garlic plants in Korea.
Lee, Eun-Tag,Kim, Sang-Dal 한국응용생명화학회 1999 Journal of Applied Biological Chemistry (J. Appl. Vol.42 No.1
An extracellular chitinase of the selected strong antifungal microorganism, Serratia sp. 3095, was purified by salting out, affinity adsorption, Sepadex G-100 gel fitration, Sepadex G-75 gel fitration and DEAE Sepadex A-50 chromatography. The molecular weight of the purified chitinase was estimated to be 62,000 dalton by SDS-PAGE. Optimal pH and temperature of the chitinase were pH 7.5 and 45, respectively. The enzyme retained more than 80% of the activity between pH 5.5 and pH 10.5, and below $50^{\circ}C$ but was unstable above $60^{\circ}C$, below pH 5.0. The activity of the chitinase was inhibited about 60% by $Sn^{2+}$, 40% by $Hg^{2+}$ and $Ag^+$, 70% by AHA, 40% by iodoacetate, 35% by thiourea and p-CMB, but stabilized by SDS. $K_m$ value of the purified chitinase was 3.68 mg/ml for colloidal chitin. The chitinase from Serratia sp. 3095 showed antifungal activity to Fusariurm solani.
유기인계 살충제 중독증에서의 혈청 Amylase 활성도
이수기 ( Lee Su Gi ),이은경 ( Lee Eun Gyeong ),정지인 ( Jeong Ji In ),정정희 ( Jeong Jeong Hui ),조준탁 ( Jo Jun Tag ),이진관 ( Lee Jin Gwan ) 대한내과학회 1993 대한내과학회지 Vol.44 No.4
Dressel 등이 anticholinesterase 살충제 중독으로 인한 임상적인 급성 췌장염을 보고한 이래 몇몇 증례가 있었고, 최근에는 Lankish 등에 의해 급성 췌장염이 보고되었다. 이에 본원에서도 이약물 중독으로 내원한 환자를 대상으로 췌장의 병발을 조사하여 다음과 같은 결론을 얻었다. 1) 유기인계 살충제 중독증 환자 31예중 17예(55%)에서 혈청 amylase가 정상치를 초과하였고 이 17예 중 5예(16%)에서 혈청 amylase가 500 U/dl 이상으로 급성 췌장염의 가능성을 시사하였다. 그러나 복통이나 장폐색등의 증세는 나타나지 않았다. 2) 급성 췌장염의 가능성을 보인 환자 5예에서 복부 초음파를 실시했는데, 1예에서 췌장의 체부와 미부에 부종 소견을 보였고 치료후 부종이 소실되었으며, 4예에서는 정상소견을 보였다. 3) 상승된 혈청 amylase는 3일이내에 거의 대부분 정상화되었으며 1예에서만 4일째 정상화되었다. 4) 의식 변화가 있으면서 기계 호흡이 필요했던 중증 중독증 환자들에게서 평균 혈청 amylase 423 U/dl로 제일 높았고, 혈청 amylase 500 U/dl 이상으로 급성 체장염의 가능성을 시사한 것도 4예로 가장 많았다. 이상의 결과로 보아 이 약물 중독 환자의 반 수 이상에서 혈청 amylase치가 정상 범위를 초과하였고 1/6정도의 환자에서 500 U/dl를 초과하여 임상적 췌장염의 가능성을 시사하였다. 따라서 우리나라 농촌 지역에서 흔히 발생하는 유기인제 중독증 환자에 있어, 특히 중증 환자 치료 중에 장 폐색증이나 복막염의 병발이 있을 경우 췌장염 발생에 관심을 가져야 할 것으로 생각된다. Organophosphate insecticides are used allover the world and are household items in the rural area of the Korea. Because of their easy accessability, their ingestion either accidentally or for a suicidal purpose is common in the Korea. Acute pancreatitis occuring as a complication of an anticholinesterase insecticide intoxication was first reported by Dressel et al(1979) and has since been reported by other investigators. Among them, pancreatitis with unresponsiveness followed by pseudocyst, or painless hemorrhagic pancreatitis only menifested by ileus and abcominal distension was founded after intoxication. We had serial serum amylase determination and clinical evaluation on 31 patients of organophosphate intoxication. The results were as follows. In the 31 patients of poisonous organophosphate insecticide, serum amylase was elevated in the 17 patients and was assumed to show pancreatic involvement. Btu, abdominal pain or ileus was absent. In 5 of 17 patients, serum amylase level was more than 500 U/dl and suggested to evoke acute pancreatitis. All of the 5 patients receive upper abdominal ultrasonography. In one patient, there was an edematious change in the body and tail of the pancreas and normalized in the following study. Others were normal. Severely affected patients who needed respiratory assistance and had mental change, mean serum amylases value was the hightest and the possibility of acute pancreatitis was the greatest. In our study, forfunately, all the patients discharged without complication of acute pancreatitis. Therefore, in the patients of organophosphate insecticide intoxication, if ileus or suspicious peritonitis is presents, we must consider the development of acute pancreatitis.