miR-133a-3p and miR-145-5p co-promote goat hair follicle stem cell differentiation by regulating NANOG and SOX9 expression

Wang Jian,Wu Xi,Zhang Liuming,Wang Qiang,Sun Xiaomei,Ji Dejun,Li Yongjun 아세아·태평양축산학회 2024 Animal Bioscience Vol.37 No.4

Objective: Hair follicle stem cells (HFSCs) differentiation is a critical physiological progress in skin hair follicle (HF) formation. Goat HFSCs differentiation is one of the essential processes of superior-quality brush hair (SQBH) synthesis. However, knowledge regarding the functions and roles of miR-133a-3p and miR-145-5p in differentiated goat HFSCs is limited. Methods: To examine the significance of chi-miR-133a-3p and chi-miR-145-5p in differentiated HFSCs, overexpression and knockdown experiments of miR-133a-3p and miR145-5p (Mimics and Inhibitors) separately or combined were performed. NANOG, SOX9, and stem cell differentiated markers (β-catenin, C-myc, Keratin 6 [KRT6]) expression levels were detected and analyzed by using real-time quantitative polymerase chain reaction, western blotting, and immunofluorescence assays in differentiated goat HFSCs. Results: miR-133a-3p and miR-145-5p inhibit NANOG (a gene recognized in keeping and maintaining the totipotency of embryonic stem cells) expression and promote SOX9 (an important stem cell transcription factor) expression in differentiated stem cells. Functional studies showed that miR-133a-3p and miR-145-5p individually or together overexpression can facilitate goat HFSCs differentiation, whereas suppressing miR-133a-3p and miR-145- 5p or both inhibiting can inhibit goat HFSCs differentiation. Conclusion: These findings could more completely explain the modulatory function of miR-133a-3p and miR-145-5p in goat HFSCs growth, which also provide more understandings for further investigating goat hair follicle development.

Efficient Mean-Shift Tracking Using an Improved Weighted Histogram Scheme

( Dejun Wang ),( Kai Chen ),( Weiping Sun ),( Shengsheng Yu ),( Hanbing Wang ) 한국인터넷정보학회 2014 KSII Transactions on Internet and Information Syst Vol.8 No.6

An improved Mean-Shift (MS) tracker called joint CB-LBWH, which uses a combined weighted-histogram scheme of CBWH (Corrected Background-Weighted Histogram) and LBWH (likelihood-based Background-Weighted Histogram), is presented. Joint CB-LBWH is based on the notion that target representation employs both feature saliency and confidence to form a compound weighted histogram criterion. As the more prominent and confident features mean more significant for tracking the target, the tuned histogram by joint CB-LBWH can reduce the interference of background in target localization effectively. Comparative experimental results show that the proposed joint CB-LBWH scheme can significantly improve the efficiency and robustness of MS tracker when heavy occlusions and complex scenes exist.

WHEN AN $\mathfrak{S}$-CLOSED SUBMODULE IS A DIRECT SUMMAND

Wang, Yongduo,Wu, Dejun Korean Mathematical Society 2014 대한수학회보 Vol.51 No.3

It is well known that a direct sum of CLS-modules is not, in general, a CLS-module. It is proved that if $M=M_1{\oplus}M_2$, where $M_1$ and $M_2$ are CLS-modules such that $M_1$ and $M_2$ are relatively ojective (or $M_1$ is $M_2$-ejective), then M is a CLS-module and some known results are generalized.

WHEN AN S-CLOSED SUBMODULE IS A DIRECT SUMMAND

Yongduo Wang,Dejun Wu 대한수학회 2014 대한수학회보 Vol.51 No.3

It is well known that a direct sum of CLS-modules is not, in general, a CLS-module. It is proved that if M = M1 ⓧ M2, where M1 and M2 are CLS-modules such that M1 and M2 are relatively ojective (or M1 is M2-ejective), then M is a CLS-module and some known results are generalized.

The enhancement of oxygen sensitivity of ZnO macropore film by functionalizing with azo pigment

Peng, Liang,Zhai, Jiali,Wang, Dejun,Zhang, Yu,Zhao, Qidong,Wang, Ping,Pang, Shan,Fan, Zhiyong,Xie, Tengfeng Korean Society of Photoscience 2009 Photochemical & photobiological sciences Vol.8 No.6

Azo-ZnO hybrid films were prepared by functionalizing the ZnO macropore films with azo pigment (1,1'-(biphenyl-4,4'-diylbis(diazene-2,1-diyl))dinaphthalen-2-ol). Scanning electronic microscopy, current-voltage, UV-Vis absorption spectroscopy and Kelvin probe were used to characterize these films. The oxygen sensing characteristics of hybrid films and pure ZnO film were measured under the irradiation of UV light. The results show that the sensitivity of hybrid film is about 500 times higher than that of pure ZnO film. The high sensitivity to oxygen of hybrid films is mainly attributed to the increasing of the photo-generated electron concentration. Enhanced photo-induced charge separation after functionalization is confirmed by the pattern of transient photovoltage. Our results demonstrate that the functionalization with azo pigment is a promising approach to enhance the oxygen sensitivity of ZnO under the irradiation of UV light.

Superwetting TiO2-decorated single-walled carbon nanotube composite membrane for highly efficient oil-in-water emulsion separation

Yahong Sun,Ruiguang Zhao,Quanyong Wang,Yuanyuan Zheng,Gongrang Li,Dejun Sun,Tao Wu,Yujiang Li 한국화학공학회 2020 Korean Journal of Chemical Engineering Vol.37 No.11

With the advantages of one-dimensional hollow structure, high porosity and prominent mechanical strength, single-walled carbon nanotubes (SWCNTs) have been extensively utilized to improve conventional filtration membranes for oil/water separation. Their intrinsic hydrophobicity, however, adversely affects the anti-fouling performance of the SWCNT membrane. Herein, a super-hydrophilic and underwater super-oleophobic hierarchical modified membrane with enhanced permeability and anti-fouling property was fabricated using the vacuum-assisted filtration technique by synergistically assembling SWCNTs and titanium dioxide (TiO2) nanoparticles on a cellulose acetate membrane. Highly dispersed SWCNTs were obtained by carboxylating treatment of agglomerate SWCNTs. The controlled stacking of SWCNTs fibers and a controllable amount of TiO2 rendered a modified membrane with high porosity and hierarchical structure, leading to an ultrahigh water flux up to 4,777.07 L·m2·h1, and excellent separation performance with efficiency greater than 99.47%. Most importantly, the membrane exhibited excellent anti-fouling ability during ten cycles with the aid of the super-wetting property of TiO2 nanoparticles. The results indicated that coating TiO2 nanoparticles on SWCNTs modified the surface topography of the obtained SWCNT/TiO2 membrane, which improved hydrophilicity, permeability and anti-fouling property, manifesting attractive potential applications in oil/water separation.

PV2JAVA: Automatic Generator of Security Protocol Implementations Written in Java Language from the Applied PI Calculus Proved in the Symbolic Model

Bo Meng,Yitong Yang,Jinli Zhang,Jintian Lu,Dejun Wang 보안공학연구지원센터 2016 International Journal of Security and Its Applicat Vol.10 No.11

In order to get the security protocol implementations written in programming language from formal languages in secure way, firstly, the model of implementation generation from security protocol implementations written in formal language is presented; Apart from that, an automatic generator PV2JAVA is developed, which can transform security protocol implementations written in the Applied PI calculus proved in the symbolic model into security protocol implementations written in Java language ; Finally, the method of software testing is used to provide a strong confidence in the correctness of the automatic generator PV2JAVA through five typical security protocols.

Identification and evaluation of reference genes for gene expression analysis in the weevil pest Pagiophloeus tsushimanus using RT-qPCR

Chen Cong,Li Shouyin,Zhu Han,Fan Binqi,Wang Yan,Hao Dejun 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.2

Pagiophloeus tsushimanus is a newly and specialist wood-boring beetle of Cinnamomum camphora in China. RTqPCR is an accurate quantitative method to quantify target genes expression, which relies on suitable reference genes for data normalization. Reference genes must to be stably expressed under specific experimental conditions. No suitable reference genes of P. tsushimanus have been reported so far. Therefore, it is necessary to identify and evaluate suitable reference genes for the study of functional genes of this pest. In this research, the expression stability of eight candidate reference genes (RPS3, 18S rRNA, GAPDH, TBP, RPL10, UBQ, GST, and RPS27A) were systematically evaluated in P. tsushimanus by five algorithms (geNorm, BestKeeper, NormFinder, delta C q , and RefFinder) under different developmental stages, various tissues, and insects reared on different plants, and validated by the olfactory key gene odorant binding protein 33 (PtsuOBP33). The results showed that three stable reference genes combination were necessary for quantitative analysis of target gene. RPS3, RPL10, and UBQ were the optimal reference genes combination for gene expression analysis of developmental stages, while RPL10, RPS3, and 18S rRNA were recommended for different tissues, and 18S rRNA, TBP, and RPS3 were recommended for insects reared on different plants. The results indicated that suitable reference genes should be screened out for gene expression analysis under different conditions. This paper systematically analyzed and obtained suitable reference genes in P. tsushimanus for the first time, which would contribute to the functional analysis of genes and the in-depth mining of genetic resources in it.

Development and Validation of a Prognostic Nomogram Based on Clinical and CT Features for Adverse Outcome Prediction in Patients with COVID-19

Zheng Yingyan,Xiao Anling,Yu Xiangrong,Zhao Yajing,Lu Yiping,Li Xuanxuan,Mei Nan,She Dejun,Wang Dongdong,Geng Daoying,Yin Bo 대한영상의학회 2020 Korean Journal of Radiology Vol.21 No.8

Objective: The purpose of our study was to investigate the predictive abilities of clinical and computed tomography (CT) features for outcome prediction in patients with coronavirus disease (COVID-19). Materials and Methods: The clinical and CT data of 238 patients with laboratory-confirmed COVID-19 in our two hospitals were retrospectively analyzed. One hundred sixty-six patients (103 males; age 43.8 ± 12.3 years) were allocated in the training cohort and 72 patients (38 males; age 45.1 ± 15.8 years) from another independent hospital were assigned in the validation cohort. The primary composite endpoint was admission to an intensive care unit, use of mechanical ventilation, or death. Univariate and multivariate Cox proportional hazard analyses were performed to identify independent predictors. A nomogram was constructed based on the combination of clinical and CT features, and its prognostic performance was externally tested in the validation group. The predictive value of the combined model was compared with models built on the clinical and radiological attributes alone. Results: Overall, 35 infected patients (21.1%) in the training cohort and 10 patients (13.9%) in the validation cohort experienced adverse outcomes. Underlying comorbidity (hazard ratio [HR], 3.35; 95% confidence interval [CI], 1.67–6.71; p < 0.001), lymphocyte count (HR, 0.12; 95% CI, 0.04–0.38; p < 0.001) and crazy-paving sign (HR, 2.15; 95% CI, 1.03–4.48; p = 0.042) were the independent factors. The nomogram displayed a concordance index (C-index) of 0.82 (95% CI, 0.76–0.88), and its prognostic value was confirmed in the validation cohort with a C-index of 0.89 (95% CI, 0.82–0.96). The combined model provided the best performance over the clinical or radiological model (p < 0.050). Conclusion: Underlying comorbidity, lymphocyte count and crazy-paving sign were independent predictors of adverse outcomes. The prognostic nomogram based on the combination of clinical and CT features could be a useful tool for predicting adverse outcomes of patients with COVID-19.

Hospitalization Due to Asthma Exacerbation: A China Asthma Research Network (CARN) Retrospective Study in 29 Provinces Across Mainland China

Jiangtao Lin,Bin Xing,Huaping Tang,Lan Yang,Yadong Yuan,Yuhai Gu,Ping Chen,Xiaoju Liu,Jie Zhang,Huiguo Liu,Changzheng Wang,Wei Zhou,Dejun Sun,Yiqiang Chen,Zhuochang Chen,Mao Huang,Qichang Lin,Chengpin 대한천식알레르기학회 2020 Allergy, Asthma & Immunology Research Vol.12 No.3

Purpose: Details of patients hospitalized for asthma exacerbation in mainland China are lacking. To improve disease control and reduce economic burden, a large sample survey among this patient population is indispensable. This study aimed to investigate the clinical characteristics and outcomes of such patients. Methods: A retrospective study was conducted on patients hospitalized for asthma exacerbation in 29 hospitals of 29 regions in mainland China during the period 2013 to 2014. Demographic features, pre-admission conditions, exacerbation details, and outcomes were summarized. Risk factors for exacerbation severity were analyzed. Results: There were 3,240 asthmatic patients included in this study (57.7% females, 42.3% males). Only 28.0% used daily controller medications; 1,287 (39.7%) patients were not currently on inhaled corticosteroids. Acute upper airway infection was the most common trigger of exacerbation (42.3%). Patients with severe to life-threatening exacerbation tended to have a longer disease course, a smoking history, and had comorbidities such as hypertension, chronic obstructive pulmonary disease (COPD), and food allergy. The multivariate analysis showed that smoking history, comorbidities of hypertension, COPD, and food allergy were independent risk factors for more severe exacerbation. The number of patients hospitalized for asthma exacerbation varied with seasons, peaking in March and September. Eight patients died during the study period (mortality 0.25%). Conclusions: Despite enhanced education on asthma self-management in China during recent years, few patients were using daily controller medications before the onset of their exacerbation, indicating that more educational efforts and considerations are needed. The findings of this study may improve our understanding of hospital admission for asthma exacerbation in mainland China and provide evidence for decision-making.