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Fuhui Cui,Junjie Wang1,Xiangdong Gan,Dawei Yu,Ilya Vladimirovich Okulov,Qinghua Tian,Xueyi Guo 대한금속ᆞ재료학회 2023 METALS AND MATERIALS International Vol.29 No.2
Liquid Mg is capable of quickly eroding the refractory Ni-based superalloy and selectively dissolve Ni, which opens up anew avenue for the recycling of superalloy scraps for comprehensive metal reclamation. This study aims at investigating theunderlining mechanism and kinetics of the selective dissolution of Ni from Inconel 718 (one type of Ni-based superalloy)with liquid Mg, by experimenting at 750, 800, and 850 °C for various amounts of time in an inert atmosphere. This selectivedissolution process is also known as liquid metal dealloying. Results show that a permeable dealloyed layer was formedresulting from the preferential dissolution of Ni over other metallic elements (e.g., Fe, Cr, Nb, Mo, and Ti). At the dealloyingforefront, the grain boundaries of the matrix γ phase were preferentially eroded by liquid Mg. The opposite concentrationgradients of Ni (4–9 wt%) and Mg (12–18 wt%) cross the dealloyed layer suggested their counter diffusion. A higher heatingtemperature substantially increased the dealloying rate.
( Pei Pei Li ),( Dong Dong Zhang ),( Xiao Juan Wang ),( Xiao Fen Wang ),( Zong Jun Cui1 ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.1
A cellulose-degrading composite microbial system containing a mixture of microbes was previously shown to demonstrate a high straw-degrading capacity. To estimate its potential utilization as an inoculant to accelerate straw biodegradation after returning straw to the field, two cellulose-degrading composite microbial systems named ADS3 and WSD5 were inoculated into wheat straw-amended soil in the laboratory. The microbial survival of the inoculant was confirmed by a denaturing gradient gel electrophoresis (DGGE) analysis, whereas the enhancement of straw degradation in soil was assessed by measuring the mineralization of the soil organic matter and the soil cellulase activity. The results indicated that most of the DGGE bands from ADS3 were detected after inoculation into straw-amended autoclaved soil, yet only certain bands from ADS3 and WSD5 were detected after inoculation into straw-amended non-autoclaved soil during five weeks of incubation; some bands were detected during the first two weeks after inoculation, and then disappeared in later stages. Organic matter mineralization was significantly higher in the soil inoculants ADS3 and WSD5 than in the uninoculated controls during the first week, yet the enhanced degradation did not persist during the subsequent incubation. Similar to the increase in soil organic matter, the cellulase activity also increased during the first week in the ADS3 and WSD5 treatments, yet decreased during the remainder of the incubation period. Thus, it was concluded that, although the survival and performance of the two inoculants did not persist in the soil, a significant enhancement of degradation was present during the early stage of incubation.
Wang, Juan,Liu, Haoming,Wang, Haili,Cui, Mingxun,Jin, Qing,Jin, Tie,Cui, Fushun,Cui, Taihua,Liang, Chengyun,Kim, Bumsik,Li, Guanhao 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.4
An protease from Actinidia arguta for improving meat tenderness was purified, characterized from wild A. arguta fruit by ammonium sulfate precipitation, Sephdex G-25 gel filtration chromatography, and DEAE Sepharose Fast Flow ion exchange chromatography, and its activity was investigated. The purified protease was subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis to obtain a single band of protease. The protease was purified successfully, and found to have a molecular weight of 23.8 kDa (mass spectrometry). The specific activity of the purified protease reached 53,428 U/mg with a 25.5-fold purification factor and 9% activity recovery. Based on N-terminal sequencing results, the A. arguta protease was derived from the class of actinidia proteases that have an N-terminal sequence of VLPDY VDWRS AGAVV. The protease was effective for tenderizing beef and decomposing actomyosin, suggesting the potential application for improving meat tenderness.
Juan Wang,Haoming Liu,Haili Wang,Mingxun Cui,Qing Jin,Tie Jin,Fushun Cui,Taihua Cui,Cheng Yun Liang,김범식,Guanhao Li 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.4
An protease from Actinidia arguta for improving meat tenderness was purified, characterizedfrom wild A. arguta fruit by ammonium sulfate precipitation, Sephdex G-25 gel filtration chromatography,and DEAE Sepharose Fast Flow ion exchange chromatography, and its activity was investigated. Thepurified protease was subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis toobtain a single band of protease. The protease was purified successfully, and found to have a molecularweight of 23.8 kDa (mass spectrometry). The specific activity of the purified protease reached 53,428U/mg with a 25.5-fold purification factor and 9% activity recovery. Based on N-terminal sequencingresults, the A. arguta protease was derived from the class of actinidia proteases that have an Nterminalsequence of VLPDY VDWRS AGAVV. The protease was effective for tenderizing beef anddecomposing actomyosin, suggesting the potential application for improving meat tenderness.
An Brain Image Segmentation Method based on Non-local MRF
Zhongyuan Cui,Feng Wang,Jin Wang 보안공학연구지원센터 2014 International Journal of Signal Processing, Image Vol.7 No.5
Brain image segmentation is one of the most important parts of clinical diagnostic tools. However, accurate segmentation of brain images is a very difficult task due to the noise, inhomogeneity and sometimes deviation in brain images. Wells model incorporates the brain image segmentation and inhomogeneity correction into one framework to overcome influences from the intensity inhomogeneity and obtain good segmentation performance. However, the classical Wells model did not take spatial information into account, so its segmentation results are sensitive to the noise. In order to overcome this limitation, the MRF theory and the nonlocal information are used to construct a nonlocal Markov Random Field. With this nonlocal MRF, the improved Wells method can obtain much better segmentation results. The experimental results show that our method is robust to the noise and is able to simultaneously keep the image edge and slender topological structure very well.
Cui, Hui-Wang,Kuo, Shiao-Wei 한국유변학회 2014 Korea-Australia rheology journal Vol.26 No.2
In this study, we synthesized a poly(N-isopropylacrylamide) (PNIPAm) through the polymerization of N-isopropylacrylamide in distilled water with azodiisobutyronitrile as the initiator and a bisadenine-functionalized poly(ethylene oxide) (A-PEO-A) from the reaction of adenine with a difunctionalized toluenesulfonyl-PEO. When blended together in distilled water, PNIPAm and A-PEO-A formed supramolecular aggregates stabilized through complementary multiple hydrogen bonds between the amide groups of PNIPAm and the adenine units of A-PEO-A. Agrawal integral equation and rheometry revealed the rheological kinetics of supramolecular assemblies, which were influenced significantly by the spherical micelles, large associated aggregates of spherical micelles, network structures, and toroid structures formed in aqueous solutions.
Studies on the Hypoglycemic Effect of ginseng Polypeptide
Wang, B.X.,Yang, M.,Jin, Y.L.,Cui, X.Y.,Wang, Y. The Korean Society of Ginseng 1990 Journal of Ginseng Research Vol.14 No.2
The ginseng Polypeptide (GPP) Isolated from the root of Panax ginseng C.A. Meyer was domonstrated to decrease the levels of blood sugar and hepatic glycogen when injected intravenoilsly to rats at a doses of 50-200 mg/kg without affecting blood total lipid. When mice were injected slibclitaneollsly daily at a dose of 50 and 100 mg/kg for 7 successive days. GPP was also found to decrease blood sligar and hepatic glycogen. In addition, GPP was found to decrease variolls experimental hypergly cemias induced by injection of adrenaline, glilcose and alloxan. GPP exhibited inhibiting effect on the glut rogen enhancement indllced by glucose, but strenthening effect on the glycogen decrease indliced by adrenaline. When the levels of blood total lipid and lilrer glycogen were increased by T alloxan. GPP was shown to inhibit these changes except its lowering blood sugar. The toxicity of GPP is very low, LD50 was found to be 1.62 $\pm$ 0.130 g/kg for iv.
Role of Nucleation-Promoting Factors in Mouse Early Embryo Development
Wang, Qiao-Chu,Liu, Jun,Wang, Fei,Duan, Xing,Dai, Xiao-Xin,Wang, Teng,Liu, Hong-Lin,Cui, Xiang-Shun,Sun, Shao-Chen,Kim, Nam-Hyung Cambridge University Press 2013 Microscopy and microanalysis Vol.19 No.3
<B>Abstract</B><P>During mitosis nucleation-promoting factors (NPFs) bind to the Arp2/3 complex and activate actin assembly. JMY and WAVE2 are two critical members of the NPFs. Previous studies have demonstrated that NPFs promote multiple processes such as cell migration and cytokinesis. However, the role of NPFs in development of mammalian embryos is still unknown. Results of the present study show that the NPFs JMY and WAVE2 are critical for cytokinesis during development of mouse embryos. Both JMY and WAVE2 are expressed in mouse embryos. After injection of JMY or WAVE2 siRNA, all embryos failed to develop to the morula or blastocyst stages. Moreover, using fluorescence intensity analysis, we found that the expression of actin decreased, and multiple nuclei were observed within a single cell indicating that NPFs-induced actin reduction caused the failure of cell division. In addition, injection of JMY and WAVE2 siRNA also caused ARP2 degradation, indicating that involvement of NPFs in development of mouse embryos is mainly through regulation of ARP2/3-induced actin assembly. Taken together, these data suggested that WAVE2 and JMY are involved in development of mouse embryos, and their regulation may be through a NPFs-Arp2/3-actin pathway.</P>