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이사겸,김명진,김창섭,김대진 경희대학교 사회체육연구소 1996 體育科學論叢 Vol.- No.9
This Study attempted to seek a method for revitalizing the community sports for al related to the sports facilities in order to encourage sports activity according with the regional property. In order to make al well-balanced distribution and untilization of sports for all facilities in Korea a comprehensive evaluation for the demand and trend in the future should be carried out. The expansion of sport facilities for the pubilc, private and one's place of work should be induced and a joint system with sport facilities for schools should be settled. The continuous investment and interacting is more important for the harmonious develolpment in each community than that of unbalanced investment.
Transcriptional Regulation of Glutathione Synthetase in the Fission Yeast Schizosaccharomyces pombe
Chang-Jin Lim,Su-Jung Kim,Hong-Gyum Kim,Byung-Chul Kim,박은희 한국분자세포생물학회 2004 Molecules and cells Vol.18 No.2
Glutathione (GSH), an important antioxidant involved in the stress response, is synthesized in two sequential reactions involving glutamylcysteine synthetase (GCS), followed by glutathione synthetase (GS). Expression of the unique GS gene in the fission yeast Schizosaccharomyces pombe was previously found to be regulated by nitric oxide and by L-buthionine-(S,R)- sulfoximine (BSO), a specific inhibitor of GCS. In this work, expression of S. pombe GS gene is shown to be induced by menadione (MD), which generates superoxide. The responsible DNA sequence between -365 and -234 bp from the translation start site, was convinced using five GS-lacZ fusion plasmids. Expression of GS gene is also induced by low glucose, fructose and disaccharides, apparently dependent on Pap1 protein; GS mRNA increases in low concentrations of glucose in wild type S. pombe but not in Pap1-negative cells. Although nonfermentable carbon sources such as acetate and ethanol stimulate expression of GS gene, they also arrest the growth of the yeast cells. These results indicate that the biosynthesis of glutathione is regulated by superoxide radicals and carbon source limitation.
Kim Hong-Gyum,Kim Byung-Chul,Kim Kyunghoon,Park Eun-Hee,Lim Chang-Jin The Microbiological Society of Korea 2004 The journal of microbiology Vol.42 No.4
In a previous study, a gst gene was isolated from the fission yeast Schizosaccharomyces pombe. This gene was dubbed gstI, and was characterized using the gstI -lacZ fusion plasmid pYSH2000. In this work, four additional fusion plasmids, pYSHSDl, pYSHSD2, pYSHSD3 and pYSHSD4, were constructed, in order to carry (respectively) 770, 551, 358 and 151 bp upstream regions from the translational initiation point. The sequence responsible for induction by aluminum, mercury and hydrogen peroxide was located in the range between -1,088 and -770 bp upstream of the S. pombe gstI gene. The same region was identified to contain the nucleotide sequence responsible for regulation by Papl, and has one putative Papl binding site, TTACGTAT, located in the range between $-954\~-947$ bp upstream of the gstI gene. Negatively acting sequences are located between -1,088 and -151 bp. These findings imply that the Papl protein is involved in basal and inducible transcription of the gstI gene in the fission yeast S. pombe.
Kim, Su-Jung,Kim, Hong-Gyum,Kim, Byung-Chul,Kim, Kyunghoon,Park, Eun-Hee,Lim, Chang-Jin The Microbiological Society of Korea 2004 The journal of microbiology Vol.42 No.3
Transcriptional regulation of the Schizosaccharomyces pombe y-glutamylcysteine synthetase (GCS) gene was examined using the two GCS-lacZ fusion plasmids pUGCS101 and pUGCS102, which harbor 607 bp and 447 bp upstream regions, respectively. The negatively-acting sequence was located in the -607 - -447 bp upstream region of the GCS gene. The upstream sequence responsible for induction by menadione(MD) and L-buthionine-(S, R)-sulfoximine (BSO) resides in the -607 - -447 bp region, whereas the sequence which codes for nitric oxide induction is located within the -447 bp region, measured from the translational initiation point. Carbon source-dependent regulation of the GCS gene appeared to be dependent on the nucleotide sequence within -447 bp region. The transcription factor Papl is involved in the induction of the GCS gene by MD and BSO, but not by nitric oxide. Induction of the GCS gene occurring due to low glucose concentration does not depend on the presence of Pap1. These data imply that induction by MD and BSO may be mediated by the Pap1 binding site, probably located in the -607 - -447 region, and also that the nitric oxide-mediated regulation of the S. pombe GCS gene may share a similar mechanism with its carbon-dependent induction.
웹 애플리케이션 기반 UI 패턴 모델 개발과 효용성에 관한 연구
김창겸 ( Chang Gyum Kim ),유승연 ( Seong Yeon Yu ),임재현 ( Jae Hyun Lim ),신택규 ( Tack Kyu Shin ),유병인 ( Byung In Yu ) LGCNS 엔트루정보기술연구소 2009 Entrue Journal of Information Technology Vol.8 No.1
최근 국내외 소프트웨어 개발 프로젝트의 특성인 잦은 요구사항의 변경, 단축된 비즈니스 라이프사이클, 비용절감의 압력등과 맞물려 생산성을 높이기 위한 개발 접근방식이 요구되었으며, 이에 소프트웨어의 재사용 및 높은 생산성을 제공할 수 있는 대표적인 방법이 디자인 패턴이다. 본 연구는 웹 애플리케이션 시스템 개발에 실제 적용 가능한 UI 디자인 패턴모델을 개발하기 위해 20개의 웹 애플리케이션 시스템을 표본으로 추출하여 1000여 개의 단위업무화면을 분석하였다. 또한 개발한 UI 패턴 모델을 실무 전문가(UI 디자이너, 개발자)들에게 이해도, 사용 적합성, 생산성 측면에서 검증하여 실무에 적용 가능한 모델임을 객관적으로 증명하였다. Here has been increase in the needs for a new development method in order to satisfy the conditions of software development projects such as frequent changes in demands, reduced business lifecycle, reduction in costs and so on. The representative method that can provide reuse and high productivity of softwares is the design pattern. This study analyzed about 1000 sample pages from 20 web application systems to develop a UI design pattern model that can be applied to development of web application systems. Also, the experts` (UI designers, developers) evaluation on the model objectively proved that it is applicable in all areas such as understanding, compatibility and productivity effect.