A型 인플루엔자에 對한 血淸疫學的 調査

白承福,張起峰,崔憲 國立保健硏究院 1973 국립보건연구원보 Vol.10 No.-

한국에서 유행하는 �냑鵝뵉퓌竄叢�관한 혈청역학적 연구

오대규,심재철,백승복,최강원,정의범,정태화,장우현,윤형희,오희복 대한감염학회 1987 감염 Vol.19 No.4

A seroepidemiological survey was performed to estimate the prevalence of leptospirosis in Korean population. The study population consisted of 967 randomly selected people from 17 counties out of 5 provinces. Macroscopic slide agglutination test was used for serology of leptospirosis. 117 sera out of 967 were positive (11.7%) for leptospirosis. Kang Won Province showed highest positive rate (17.1%), followed by Kyunggi (16.2%), Chung Book (10.2%), Kyung Book (6.2%), Chun Nam Province (2.2%), Hoeng Sung County showed highest prevalence rate (26.2%) and Chang Sung County Showed lowest prevalence (0%). There was no difference in prevalence rate between male and female. But older people (41∼60 years) showed higher prevalence rate than the younger people (21∼30 years). Some activities, including weeding in the field or grave and working barefoot in the rice field were associated with higher prevalence. History of recent flu-like symptoms suggestive of leptospirosis (chill, headache, cough, walking difficulty, dyspnea, diarrhea, conjunctival suffusion) was more common in antibody positive group. Leptospirosis is wide spread infection throughout Korea, though considerable variance in prevalence rate was noted.

Sparganum mansoni의 腹腔 및 腹壁皮下寄生 各 1例

심명석,서동엽,장석효,김홍용,백인욱,이혁상,백낙환 인제대학교 1982 仁濟醫學 Vol.3 No.2

Clinical experience with a case of sparganosis in the abdominal wall, and another one in the abdominal cavity is reported. A living larva of the Sparganum mansoni was removed from the subcutaneous tissue of the abdominal wall of the 62 year-old female. Biopsy of a nodule from the serosal surface of the 22-year old male revealed a eosinophilic granulomatous lesion, which contained its dead larva.

Biodistribution of 99mTc Labeled Integrin Antagonist

장범수,Seung-Hee Park,InSooShin,Jin-Soo Maeng,Chang H. Paik 한국독성학회 2013 Toxicological Research Vol.29 No.1

The selective targeting of an integrin αvβ3 receptor using radioligands may enable the assessment of angiogenesis and integrin αvβ3 receptor status in tumors. The aim of this research was to label a peptidomimetic integrin αvβ3 antagonist (PIA) with 99mTc(CO)3 and to test its receptor targeting properties in nude mice bearing receptor-positive tumors. PIA was reacted with tris-succinimidyl aminotriacetate (TSAT) (20 mM) as a PIA per TSAT. The product, PIA-aminodiacetic acid (ADA), was radiolabeled with [99mTc(CO)3(H2O)3]+1,and purified sequentially on a Sep-Pak C-18 cartridge followed by a Sep-Pak QMA anion exchange cartridge. Using gradient C-18 reverse-phase HPLC, the radiochemical purity of 99mTc(CO)3-ADA-PIA (retention time, 10.5 min) was confirmed to be > 95%. Biodistribution analysis was performed in nude mice (n = 5 per time point) bearing receptor-positive M21 human melanoma xenografts. The mice were administered 99mTc(CO)3-ADA-PIA intravenously. The animals were euthanized at 0.33, 1, and 2 hr after injection for the biodistribution study. A separate group of mice were also co-injected with 200 μg of PIA and euthanized at 1 hr to quantify tumor uptake. 99mTc(CO)3-ADA-PIA was stable in phosphate buffer for 21 hr, but at 3 and 6 hr, 7.9 and 11.5% of the radioactivity was lost as histidine, respectively. In tumor bearing mice,99mTc(CO)3-ADA-PIA accumulated rapidly in a receptor-positive tumor with a peak uptake at 20 min, and rapid clearance from blood occurring primarily through the hepatobiliary system. At 20 min, the tumor-toblood ratio was 1.8. At 1 hr, the tumor uptake was 0.47% injected dose (ID)/g, but decreased to 0.12% ID/g when co-injected with an excess amount of PIA, indicating that accumulation was receptor mediated. These results demonstrate successful 99mTc labeling of a peptidomimetic integrin antagonist that accumulated in a tumor via receptor-specific binding. However, tumor uptake was very low because of low blood concentrations that likely resulted from rapid uptake of the agent into the hepatobiliary system. This study suggests that for 99mTc(CO)3-ADA-PIA to be useful as a tumor detection agent, it will be necessary to improve receptor binding affinity and increase the hydrophilicity of the product to minimize rapid hepatobiliary uptake.

Biodistribution of <SUP>99m</SUP>Tc Labeled Integrin Antagonist

Beom-Su Jang,Seung-Hee Park,In Soo Shin,Jin-Soo Maeng,Chang H. Paik 한국독성학회 2013 Toxicological Research Vol.29 No.1

The selective targeting of an integrin αvβ3 receptor using radioligands may enable the assessment of angiogenesis and integrin αvβ3 receptor status in tumors. The aim of this research was to label a peptidomimetic integrin αvβ3 antagonist (PIA) with <SUP>99m</SUP>Tc(CO)3 and to test its receptor targeting properties in nude mice bearing receptor-positive tumors. PIA was reacted with tris-succinimidyl aminotriacetate (TSAT) (20 mM) as a PIA per TSAT. The product, PIA-aminodiacetic acid (ADA), was radiolabeled with [<SUP>99m</SUP>Tc(CO)3(H2O)3]<SUP>+1</SUP>, and purified sequentially on a Sep-Pak C-18 cartridge followed by a Sep-Pak QMA anion exchange cartridge. Using gradient C-18 reverse-phase HPLC, the radiochemical purity of <SUP>99m</SUP>Tc(CO)3-ADA-PIA (retention time, 10.5 min) was confirmed to be > 95%. Biodistribution analysis was performed in nude mice (n = 5 per time point) bearing receptor-positive M21 human melanoma xenografts. The mice were administered <SUP>99m</SUP>Tc(CO)3-ADA-PIA intravenously. The animals were euthanized at 0.33, 1, and 2 hr after injection for the biodistribution study. A separate group of mice were also co-injected with 200 μg of PIA and euthanized at 1 hr to quantify tumor uptake. <SUP>99m</SUP>Tc(CO)3-ADA-PIA was stable in phosphate buffer for 21 hr, but at 3 and 6 hr, 7.9 and 11.5% of the radioactivity was lost as histidine, respectively. In tumor bearing mice, <SUP>99m</SUP>Tc(CO)3-ADA-PIA accumulated rapidly in a receptor-positive tumor with a peak uptake at 20 min, and rapid clearance from blood occurring primarily through the hepatobiliary system. At 20 min, the tumor-toblood ratio was 1.8. At 1 hr, the tumor uptake was 0.47% injected dose (ID)/g, but decreased to 0.12% ID/g when co-injected with an excess amount of PIA, indicating that accumulation was receptor mediated. These results demonstrate successful <SUP>99m</SUP>Tc labeling of a peptidomimetic integrin antagonist that accumulated in a tumor via receptor-specific binding. However, tumor uptake was very low because of low blood concentrations that likely resulted from rapid uptake of the agent into the hepatobiliary system. This study suggests that for <SUP>99m</SUP>Tc(CO)3-ADA-PIA to be useful as a tumor detection agent, it will be necessary to improve receptor binding affinity and increase the hydrophilicity of the product to minimize rapid hepatobiliary uptake.

Biodistribution of ^99mTc Labeled Integrin Antagonist

Jang, Beom-Su,Park, Seung-Hee,Shin, In Soo,Maeng, Jin-Soo,Paik, Chang H. Korean Society of ToxicologyKorea Environmental Mu 2013 Toxicological Research Vol.29 No.1

The selective targeting of an integrin ${\alpha}_v{\beta}_3$ receptor using radioligands may enable the assessment of angiogenesis and integrin ${\alpha}_v{\beta}_3$ receptor status in tumors. The aim of this research was to label a peptidomimetic integrin ${\alpha}_v{\beta}_3$ antagonist (PIA) with $^{99m}Tc(CO)_3$ and to test its receptor targeting properties in nude mice bearing receptor-positive tumors. PIA was reacted with tris-succinimidyl aminotriacetate (TSAT) (20 mM) as a PIA per TSAT. The product, PIA-aminodiacetic acid (ADA), was radiolabeled with $[^{99m}Tc(CO)_3(H_2O)_3]^{+1}$, and purified sequentially on a Sep-Pak C-18 cartridge followed by a Sep-Pak QMA anion exchange cartridge. Using gradient C-18 reverse-phase HPLC, the radiochemical purity of $^{99m}Tc(CO)_3$-ADA-PIA (retention time, 10.5 min) was confirmed to be > 95%. Biodistribution analysis was performed in nude mice (n = 5 per time point) bearing receptor-positive M21 human melanoma xenografts. The mice were administered $^{99m}Tc(CO)_3$-ADA-PIA intravenously. The animals were euthanized at 0.33, 1, and 2 hr after injection for the biodistribution study. A separate group of mice were also co-injected with 200 ${\mu}g$ of PIA and euthanized at 1 hr to quantify tumor uptake. $^{99m}Tc(CO)_3$-ADA-PIA was stable in phosphate buffer for 21 hr, but at 3 and 6 hr, 7.9 and 11.5% of the radioactivity was lost as histidine, respectively. In tumor bearing mice, $^{99m}Tc(CO)_3$-ADA-PIA accumulated rapidly in a receptor-positive tumor with a peak uptake at 20 min, and rapid clearance from blood occurring primarily through the hepatobiliary system. At 20 min, the tumor-to-blood ratio was 1.8. At 1 hr, the tumor uptake was 0.47% injected dose (ID)/g, but decreased to 0.12% ID/g when co-injected with an excess amount of PIA, indicating that accumulation was receptor mediated. These results demonstrate successful $^{99m}TC$ labeling of a peptidomimetic integrin antagonist that accumulated in a tumor via receptor-specific binding. However, tumor uptake was very low because of low blood concentrations that likely resulted from rapid uptake of the agent into the hepatobiliary system. This study suggests that for $^{99m}Tc(CO)_3$-ADA-PIA to be useful as a tumor detection agent, it will be necessary to improve receptor binding affinity and increase the hydrophilicity of the product to minimize rapid hepatobiliary uptake.

Covalent attachment of polystyrene on multi-walled carbon nanotubes via nitroxide mediated polymerization

Chang, J. H.,Lee, Y. W.,Kim, B. G.,Kim, H.-K.,Choi, I. S.,Paik, H.-J. V.S.P. 2007 COMPOSITE INTERFACES -ZEIST- Vol.14 No.5-6

QUALITY ASSURANCE IN RADIOPHARMACEUTICALS

Paik, Chang H. 대한핵의학회 1984 핵의학 분자영상 Vol.18 No.1

Optimal Lysine:DE Ratio for Growing Pigs of Different Sexes

Chang, W.H.,Kim, J.D.,Xuan, Z.N.,Cho, W.T.,Han, In K.,Chae, B.J.,Paik, In K. Asian Australasian Association of Animal Productio 2000 Animal Bioscience Vol.13 No.1

This study was conducted to evaluate changes in the lysine to digestible energy (DE) ratio on performance, apparent ileal and fecal nutrient digestibilities as well as blood urea nitrogen (BUN), and to estimate optimal lysine:DE ratios for growing pigs of different sexes. A total of 150 pigs ($(Landrace{\times}Yorkshire){\times}Duroc$, 16.78 kg average body weight, 75 barrows and 75 gilts) was randomly allotted into a $2{\times}3$ (sex by diet) factorial design. Three diets were formulated to contain a crude protein level of 19%, a DE level of 3.5 Mcal/kg with three lysine:DE ratios of 3.2 (low), 3.5 (middle) and 3.8 (high) g lysine/Mcal DE per kg diet for both barrows and gilts throughout the study. With increasing dietary lysine:DE ratio, the average daily gain (ADG) of barrows decreased but there was no significant difference among treatments (p>0.05). However, ADG was significantly higher in gilts fed the diet containing the high lysine:DE ratio (p<0.05), followed by the middle and low lysine:DE ratio dietary groups. No significant effects of lysine:DE ratios on feed intake (ADFI) and feed conversion (F/G) were observed for barrows and gilts during overall period (p>0.05), while the optimal F/G was found in barrows fed diets of low and in gilts fed high lysine:DE ratio. Blood urea nitrogen had a positive relationship with growth rate. The results showed that the optimal lysine:DE ratios were 3.2 and 3.8 g lysine/Mcal DE per kg diet for barrows and gilts of 16 to 57 kg body weight, respectively.

Optimal Threonine:Lysine Ratio for Growing Pigs of Different Sexes

Chang, W.H.,Lee, J.H.,Heo, K.N.,Paik, I.K.,Han, In K. Asian Australasian Association of Animal Productio 2000 Animal Bioscience Vol.13 No.12

This study was conducted to investigate the effects of threonine:lysine ratios on growth performance, apparent nutrient digestibility and blood urea nitrogen (BUN) concentration, and to estimate the optimal threonine:lysine ratios for growing barrows and gilts. A total of 150 pigs (Landrace${\times}$Yorkshire${\times}$Duroc, $16.75{\pm}0.42kg$ average body weight, 75 barrows and 75 gilts) was randomly allotted into six treatments in a $2{\times}3$ factorial design. Six diets were formulated to contain 1.12% lysine for barrows and 1.33% lysine for gilts with three threonine:lysine ratios (50, 60 and 70%) for both barrows and gilts. Throughout the whole experimental period (16 to 56 kg body weight), there was no interaction between sex and dietary threonine:lysine ratio in average daily gain (ADG), average daily feed intake (ADFI) and feed conversion rate (FCR). Between sexes, there was a clear sex-effect showing better growth performance of barrows. Barrows consumed more feed (p<0.01) and grew faster (p<0.01) than gilts. For barrows, there was a trend to improved ADG and FCR with increasing threonine:lysine ratio. For gilts, there was a trend to improved ADG and FCR up to threonine:lysine ratio of 60%, but not significant. There was no interaction between sex and threonine:lysine ratio in nutrient digestibilities of growing pigs except for crude ash (CA). Between sexes, there were differences in nutrient digestibilities, except for calcium for which gilts showed higher a digestibility (p<0.01). Among dietary threonine:lysine ratios, there were no differences in nutrient digestibilities. Mean values of essential amino acids (EAA), non-essential amino acids (NEAA) and total amino acids (TAA) digestibilities were not affected by sex and dietary threonine:lysine ratio. There was no evidence of an interaction between sexes and dietary threonine:lysine ratio. Between sexes, total BUN concentration was lower in gilts than barrows (p<0.05). It was concluded that a 70 and 60% dietary threonine:lysine ratio for barrows (1.12% lysine) and gilts (1.33% lysine) tended to result in better growth performances and nutrient utilization and lower BUN concentration than other threonine:lysine ratios.