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Park Dong-Geun,Ha Eun-Su,Kang Byungcheol,Choi Iseul,Kwak Jeong-Eun,Choi Jinho,Park Jeongwoong,이우정,Kim Seung Hwan,Kim Soon Han,이주훈 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.1
These days, bacterial detection methods have some limitations in sensitivity, specificity, and multiple detection. To overcome these, novel detection and identification method is necessary to be developed. Recently, NGS panel method has been suggested to screen, detect, and even identify specific foodborne pathogens in one reaction. In this study, new NGS panel primer sets were developed to target 13 specific virulence factor genes from five types of pathogenic Escherichia coli, Listeria monocytogenes, and Salmonella enterica serovar Typhimurium, respectively. Evaluation of the primer sets using singleplex PCR, crosscheck PCR and multiplex PCR revealed high specificity and selectivity without interference of primers or genomic DNAs. Subsequent NGS panel analysis with six artificially contaminated food samples using those primer sets showed that all target genes were multi-detected in one reaction at 108 -105 CFU of target strains. However, a few false-positive results were shown at 106 -105 CFU. To validate this NGS panel analysis, three sets of qPCR analyses were independently performed with the same contaminated food samples, showing the similar specificity and selectivity for detection and identification. While this NGS panel still has some issues for detection and identification of specific foodborne pathogens, it has much more advantages, especially multiple detection and identification in one reaction, and it could be improved by further optimized NGS panel primer sets and even by application of a new real-time NGS sequencing technology. Therefore, this study suggests the efficiency and usability of NGS panel for rapid determination of origin strain in various foodborne outbreaks in one reaction.
Realistic and aggregated exposure assessment of Korean men and women to color make-up products
Jung, Kyungeun,Choi, Misoo,Hong, Seungphil,Park, Byungcheol,Park, Ilyong,Shin, Jeonghyun,Lee, Taewon,Park, Gyeong-Hun,Kim, Myunghwa Elsevier 2018 Food and chemical toxicology Vol.118 No.-
<P><B>Highlights</B></P> <P> <UL> <LI> Our study provided realistic exposure data based on actual use of facial color makeup products, providing fundamental elements of exposure assessments, including amount of use, frequency of use, and various socioeconomic factors. </LI> <LI> Koreans were revealed to use smaller amounts of the color makeup products than Americans and Europeans, except for lip gloss/balm/stick, eyeliner, and eyebrow pencil. </LI> <LI> In addition, Koreans showed to use color cosmetics less frequently. </LI> </UL> </P>
Choi, Danbi,Ahn, Byungcheol,Kim, Se Hyun,Hong, Kipyo,Ree, Moonhor,Park, Chan Eon American Chemical Society 2012 ACS APPLIED MATERIALS & INTERFACES Vol.4 No.1
<P>The effects of spin speed and an amorphous fluoropolymer (CYTOP)-patterned substrate on the crystalline structures and device performance of triisopropylsilylethynyl pentacene (TIPS-PEN) organic field-effect transistors (OFETs) were investigated. The crystallinity of the TIPS-PEN film was enhanced by decreasing the spin speed, because slow evaporation of the solvent provided a sufficient time for the formation of thermodynamically stable crystalline structures. In addition, the adoption of a CYTOP-patterned substrate induced the three-dimensional (3D) growth of the TIPS-PEN crystals, because the patterned substrate confined the TIPS-PEN molecules and allowed sufficient time for the self-organization of TIPS-PEN. TIPS-PEN OFETs fabricated at a spin speed of 300 rpm with a CYTOP-patterned substrate showed a field-effect mobility of 0.131 cm<SUP>2</SUP> V<SUP>–1</SUP> s<SUP>–1</SUP>, which is a remarkable improvement over previous spin-coated TIPS-PEN OFETs.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/aamick/2012/aamick.2012.4.issue-1/am201074n/production/images/medium/am-2011-01074n_0007.gif'></P>
( Chanhee Nam ),( Minkee Park ),( Misoo Choi ),( Eunhee Choi ),( Byungcheol Park ),( Myunghwa Kim ),( Seung Phil Hong ) 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.2
Background: Low level laser therapy(LLLT) using light emitting diode(LED) is a non-thermal and non-invasivetreatment which stimulates cell activities and functions through a photobiomodulative effect. Objectives: We performed a prospective, randomized, double-blinded, comparative trial to evaluate the efficacy and safety of two LLLT devices using monochromatic 660 nm emitted red or polychromatic 411-777 nm emitted white LED on the facial wrinkles. Methods: A total of 52 adult female subjects with more than grade 3 of lateral periocular wrinkle were randomly divided into two groups, red LED and white LED group.The face of subjects was eradiated with 5 J of red LED or white LED for 12 weeks. Results: In both groups of red LED and white LED, the wrinkle measurement from skin replica on the bilateral periorbital area improved significantly from baseline at week 12. Red LED group showed slightly better improvement, but there were no statistical differences. In investigator``s assessment, the wrinkle grade showed nostatistical differences within groups as well as between groups. In subject’s global assessment, the mean improvement score of red LED group was significantly higher than that of the white LED group. Total 12 cases of adverse reactions were reported during the study period, but most of them were mild and self-limited. There were no statistical differences between two groups. Conclusion: Monochromatic 660 nm red LED could be an effective and tolerable treatment option for wrinkles.