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Bacillus amyloliquefaciens k 의 생육과 α - Amylase 생육에 대한 Tunicamycin 의 영향
전촌학조,김기철,산기진수,고월소 한국농화학회 1981 Applied Biological Chemistry (Appl Biol Chem) Vol.24 No.4
The effects of tunicamycin (TM) on the growth and α-amylase productivity of B. amyleliucfaciens K were studied. The minimal growth inhibitory concentration was 0.25㎍/㎖ and its α-amylase was stable up to 50℃. When the saking culture with 1㎍/㎖ of Tunicamycin oaused the change of cell shape from form rod to irregular circular form and the mycelium lysis. the grow th of His-. TM mutant obtained by treatment of TM and ultraviolet ray was similar to that of the parent strain, but the productivity of α-amylase, protease, and RXase was lower than that of the parent.
Gene Expression Profiling for Toxicological Assessment of Manufactured Nanoparticles in Rat Lung
Katsuhide Fujita,Yasuo Morimoto,Akira Ogami,Toshihiko Myojyo,Isamu Tanaka,Manabu Shimada,Wei-Ning Wang,Shigehisa Endoh,Kunio Uchida,Hiroaki Tao,Masaharu Inada,Tetsuya Nakazato,Moritaka Tamura,Kazuhiro 환경독성보건학회 2008 한국독성학회 심포지움 및 학술발표회 Vol.- No.-
Tang, Ning,Araki, Yuki,Tamura, Kenji,Dong, Lijun,Zhang, Xuemei,Liu, Qiuhua,Ji, Ruonan,Kameda, Takayuki,Toriba, Akira,Hayakawa, Kazuichi Korean Society for Atmospheric Environment 2009 Asian Journal of Atmospheric Environment (AJAE) Vol.3 No.1
Airborne particulates were collected in three different size fractions (>7 ${\mu}m$; 2.1-7 ${\mu}m$; < 2.1 ${\mu}m$) by using Andersen low-volume air samplers at three sites in Tieling city, Liaoning Province, a typical local city in northeast China, in every season during the period from July 2003 to May 2004. Nine polycyclic aromatic hydrocarbons (PAHs) and seven nitropolycyclic aromatic hydrocarbons (NPAHs) in the airborne particulates were determined by HPLC with fluorescence and chemiluminescence detection, respectively. The mean total concentrations of the nine PAHs and seven NPAHs were highest at The mixed residential and light industrial site, and lowest at the residential site near the suburbs. At all sites, more than 70% of the total PAHs and more than 60% of the total NPAHs were found in the finest particulate (< 2.1 ${\mu}m$) fraction. Both cluster analysis and several diagnostic ratios showed that the major contributors of PAHs and NPAHs in airborne particulates were coal combustion systems such as domestic stoves and coal boilers in all seasons in Tieling city.
Bacillus amyloliquefaciens k의 생육(生育)과 ${\alpha}-Amylase$ 생산(生産)에 대한 Tunicamycin의 영향(影響)
김기철,산기진수,고월소,전촌학조,Kim, Ki-Cheul,Yamaski, Makari,Takatsuki, Akira,Tamura, Gakuzo 한국응용생명화학회 1981 Applied Biological Chemistry (Appl Biol Chem) Vol.24 No.4
Bacillus amyloliquefaciens K의 생육(生育)과 ${\alpha}-amylase$ 생산(生産)에 대한 Tunicamycin (TM)의 영향(影響)을 검토(檢討)한 결과(結果) 최소생육저지농도(最少生育沮止濃度)는 $0.25{\mu}\textrm{g}/m\ell$이고 생산(生産)된 ${\alpha}-amylase$의 내열성(耐熱性)은 $50^{\circ}C$이었다. TM 첨가량(添加量)이 $1{\mu}\textrm{g}/m\ell$인 액체배지(液體培地)에서 10시간(時間) 진탕배양(振湯培養)할 경우 균(菌)의 형태(形態)는 간형(桿形)에서 부정형(不定形)의 구형(球形)으로 변형(變形)되었으며 24시간배양(時間培養)으로 용해(溶解)되었다. TM와 자외선의 처리로 분리(分離)한 his-, $TM^{\tau}$ 변이주(變異株)의 생육(生育)은 친주(親株)와 같은 경향(傾向) 이었으나 ${\alpha}-amylase$, protease, 및 RNase의 생산량(生産量)은 떨어지는 경향(傾向)이다. The effects of tunicamycin (TM) on the growth and ${\alpha}-amylase$ productivity of B. amyloliquefaciens K were studied. The minimal growth inhibitory concentration was $0.25{\mu}\textrm{g}/m\ell$ and its ${\alpha}-amylase$ was stable up to $50^{\circ}C$. When the saking culture with $1{\mu}\textrm{g}/m\ell$ of Tunicamycin caused the change of cell shape from form rod to irregular circular form and the mycelium lysis. the grow th of His-, $TM^{\tau}$ mutant obtained by treatment of TM and ultraviolet ray was similar to that of the parent strain, but the productivity of ${\alpha}-amylase$, protease, and RNase was lower than that of the parent.
Nozue Goro,Fujiwara Hidenori,Hamamoto Satoru,Kiss Takayuki,Tsutsumi Miwa,Oura Masaki,Ishikawa Tetsuya,Motouri Azusa,Suzuki Shintaro,Tamura Ryuji,Sekiyama Akira 한국물리학회 2023 새물리 Vol.73 No.12
We have performed Ce M4,5-edge X-ray absorption (XAS) and Ce 3d-4f resonance photoemission (RPES) spectroscopies on quasicrystal approximant Au59.2Ga25.7Ce15.1. The 3d94f1 final state originating from the itinerant 4f electronic states is negligible in the Ce M4,5-edge XAS. The Ce 3d-4f RPES spectrum shows the relatively large the spectral weight of the 4f0 final state to that of the 4f1 final state, reflecting the weak hybridization between the localized 4f orbitals and the conduction bands. The localized 4f electronic states in Au59.2Ga25.7Ce15.1 are revealed by both spectroscopies.