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        Effects of Olaquindox and Cyadox on Immunity of Piglets Orally Inoculated with Escherichia coli

        Ding, Mingxing,Yuan, Zonghui,Wang, Yulian,Zhu, Huiling,Fan, Shengxian Asian Australasian Association of Animal Productio 2005 Animal Bioscience Vol.18 No.9

        A 2${\times}$3 factorial arrangement of treatments was used to determine the effects of olaquindox and cyadox on immune response of Landrace${\times}$Large-White geld piglets that had been orally given 10$^{10}$ CFU of Escherichia coli (E. coli, O$_{139}$:K$_{88}$). Factors included (1) E. coli inoculation or control, and (2) no antimicrobials, 100 mg/kg olaquindox and 100 mg/kg cyadox in the basal diet respectively. E. coli inoculums were orally administered 7 days after the diets were supplemented with olaquindox and cyadox. The effects of the two antimicrobials were assessed in terms of: (1) average daily gain (ADG), (2) systemic immune response (the number of white blood cells and lymphocytes, leukocyte bactericidal capacity, lymphocyte proliferation response to PHA, immunoglobulin concentrations, and total serous hemolytic complement activity), and (3) intestinal mucosal immunity including the number of intraepithelial lymphocytes (IELs) and immunoglobulin A secreting cells (ASCs) in the intestinal lamina propria. E. coli inoculation reduced ADG (p<0.05) during the period of d 0 to d 14 after the challenge while the antimicrobial supplementations improved ADG (p<0.01) during the experiment. ADG in cyadox-supplemented pigs was higher (p<0.05) than that in olaquindox-supplemented pigs. The antimicrobials decreased IEL and ASC counts in the jejunum and ileum (p<0.01) while E. coli inoculation caused them to increase (p<0.01). Jejunal ASCs in the cyadox-supplemented pigs were lower (p<0.05) than those in the olaquindox-supplemented. E. coli elicited increase (p<0.05) in white blood cell counts, leukocyte bactericidal capacity, lymphocyte proliferation rate, serous IgA concentrations, and serous hemolytic complement activity. The antimicrobials decreased the measured systemic immune parameters, but not significantly (p>0.05). The data suggest that olaquindox and cyadox suppress E. coli-induced immune activation, especially intestinal mucosal immune activation, which may be involved in the observed growth promotion.

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        Oviposition preference and offspring performance of Apoderus dimidiatus (Coleoptera: Atelabidae) between Rosa multiflora and Rubus ichangensis

        Xie Meng,Du Jinying,Yang Kunqi,Song Shaoting,Lv Zonghui,Xu Huailiang,Wen An Xiang,Wang Qin,Jiang Yanzhi,Zhu Guangxiang,Wu Jiayun,Yao Yongfang,Ni Qingyong 한국곤충학회 2021 Entomological Research Vol.51 No.2

        Leaf-rolling weevils (Coleoptera: Atelabidae) are considered to be specialists that oviposit only on one or two plant species. However, Apoderus dimidiatus can oviposit on at least five species. We aimed to determine whether A. dimidiatus females have different preferences for oviposition substrates, and whether differences in physical factors, nutrition and parasitism affected their choices. We observed the number of cradles, hatched larvae, pupae, emerging adults and parasitism rate during one breeding season. Weevils were given access to two host plant species, Rosa multiflora and Rubus ichangensis, and the leaf size and nutrient contents of the two plant species were investigated. We found that nutritional content, especially soluble carbohydrate, correlated with oviposition preference of weevils, and the more nutritious plant species, R. multiflora, was the preferred host in our experiment. Weevils can also use Ru. ichangensis and other plant species as hosts, likely to relieve the high parasitic pressure when laying eggs on a single host species.

      • KCI등재

        In Vitro Development and Transfer of Resistance to Chlortetracycline in Bacillus subtilis

        Menghong Dai,Junjie Lu,Yulian Wang,Zhenli Liu,Zonghui Yuan 한국미생물학회 2012 The journal of microbiology Vol.50 No.5

        The present criteria and rules controlling the approval of the use of probiotics are limited to antibiotic resistance patterns and the presence of antibiotic resistance genes in bacteria. There is little information available in the literature regarding the risk of the usage of probiotics in the presence of antibiotic pressure. In this study we investigated the development and transfer of antibiotic resistance in Bacillus subtilis selected in vitro by chlortetracycline in a stepwise manner. Bacillus subtilis was exposed to increasing concentrations of chlortetracyclineto induce in vitro resistance to chlortetracycline, and the minimal inhibitory concentrations were determinedfor the mutants. Resistant B. subtilis were conjugated with Escherichia coli NK5449 and Enterococcus faecalis JH2-2 using the filter mating. Three B. subtilis tetracycline resistant mutants (namely, BS-1, BS-2, and BS-3)were derived in vitro. A tetracycline resistant gene, tet (K),was found in the plasmids of BS-1 and BS-2. Three conjugates (BS-1N, BS-2N, and BS-3N) were obtained when the resistant B. subtilis was conjugated with E. coli NK5449. The conjugation frequencies for the BS-1N, BS-2N, and BS-3N conjugates were 4.57×10-7, 1.4×10-7, and 1.3×10-8,respectively. The tet(K) gene was found only in the plasmids of BS-1N. These results indicate that long-term use of probiotics under antibiotic selection pressure could cause antibiotic resistance, and the resistance gene could be transferred to other bacteria. The risk arising from the use of probiotics under antibiotic pressure should be considered in the criteria and rules for the safety assessment of probiotics.

      • KCI등재

        A Case Study on the Construction Optimization Decision Scheme of Urban Subway Tunnel Based on the TOPSIS Method

        Bo Wu,Ming Lu,Wei Huang,Yangbing Lan,Yufang Wu,Zonghui Huang 대한토목학회 2020 KSCE JOURNAL OF CIVIL ENGINEERING Vol.24 No.11

        Because of the harsh environment around city subways and the typically poor geological conditions, it is often difficult to ensure the stability of a tunnel, which makes it prone to collapses and other accidents. The influence of multiple factors on tunnel stability was analyzed under the same geological conditions and environmental factors by using a three-dimensional numerical simulation. Taking into account the objective environment and manual operation and other factors, construction sequence, support closure time, and reinforcement area and parameters were chosen as the main factors affecting the tunnel stability. Finally, based on ground deformation, tunnel convergence deformation, and surrounding soil stress as evaluation indicators, a fuzzy analytic hierarchy process (FAHP)-gray correlation degree-technique for order performance by similarity to ideal solution (TOPSIS) model was established. It was found that the higher the relative closeness of the construction parameter combination, the greater the tunnel stability. The best combination of construction parameters was applied to the Tiantongyuan Station project of Beijing Metro Line 17. All geological conditions being equal, reasonable selection of construction parameters can ensure optimal tunnel construction stability, reduce construction risks, and support sustainable tunnel development. In addition, the FAHP-gray correlation degree-TOPSIS method achieved multi-index evaluation of tunnel stability, which provides practical guidance for the construction of similar projects.

      • KCI등재

        Influence of Nb on the hot corrosion behaviors of a Fe-Cr-Ni heat-resistant steel weld metal in the Na2SO4-NaCl molten salt at 700 ℃

        Zhang Xu,Wan Jinchu,Zhu liang,Zhou Hui,Yang Zonghui 대한금속·재료학회 2023 METALS AND MATERIALS International Vol.29 No.12

        A multiple gas tungsten arc welding was performed on the fabrication of the Fe–Cr–Ni-based weld metal. Microstructuresand hot corrosion behaviors of the non-equilibrium weld metals with different Nb contents in 75 wt%Na2SO4–25 wt%NaClmolten salt at 700 °C have been investigated. A bilayer oxide film composed of an inner Cr2O3layer and an outer Fe2O3layer formed on the surface of the weld metals. The mass gain rate and the thickness of the surface oxide film were enlargedwith Nb addition, which could be attributed to the rapid inward diffusion of O through the cracks resulting from the Nboxide. Oxidation and sulfidation occur in the matrix, resulting in a large internal oxidation zone (IOZ) in the weld metal. Nb seemly promoted the sulfidation reaction due to a short-circuit diffusion of S through the interface of the Nb(C, N)and matrix and made the internal corrosion products to be distributed along the interdendritic zone of the weld metal. Anapparent Cr-dealloyed layer appears in the IOZ of the weld metal. Dealloying was the detrimental form of hot corrosion ofFe–Cr–Ni-based weld metal exposed to molten salts containing chloride salts. Alloying with 0.8 wt% of Nb further acceleratedthe dealloying and resulted in a profound attack.

      • KCI등재

        Pharmacokinetics/Pharmacodynamics models of veterinary antimicrobial agents

        Wanhe Luo,Dongmei Chen,Mengru Wu,Zhenxia Li,Yanfei Tao,Qianying Liu,Yuanhu Pan,Wei Qu,Zonghui Yuan,Shuyu Xie 대한수의학회 2019 Journal of Veterinary Science Vol.20 No.5

        Misuse and abuse of veterinary antimicrobial agents have led to an alarming increase in bacterial resistance, clinical treatment failure, and drug residues. To address these problems, consistent and appropriate dosage regimens for veterinary antimicrobial agents are needed. Pharmacokinetics/Pharmacodynamics (PK/PD) models have been widely used to establish rational dosage regimens for veterinary antimicrobial agents that can achieve effective prevention and treatment of bacterial diseases and avoid the development of bacterial resistance. This review introduces building methods for PK/PD models and describes current PK/PD research progress toward rational dosage regimens for veterinary antimicrobial agents. Finally, the challenges and prospects of PK/PD models in the design of dosage regimens for veterinary antimicrobial agents are reviewed. This review will help to increase awareness of PK/PD modeling among veterinarians and hopefully promote its development and future use.

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