LncRNA A2M-AS1 lessens the injury of cardiomyocytes caused by hypoxia and reoxygenation via regulating IL1R2

Xue‑Lian Song,Fei‑Fei Zhang,Wen‑Jing Wang,Xin‑Ning Li,Yi Dang,Ying‑Xiao Li,Qian Yang,Mei‑Jing Shi,Xiao‑Yong Qi 한국유전학회 2020 Genes & Genomics Vol.42 No.12

Background: Myocardial ischemia and reperfusion injury (MI/RI) is a complex pathophysiological process, which can lead to severe myocardial injury. The long noncoding RNA alpha-2-macroglobulin antisense RNA 1 (A2M-AS1) has been revealed to be abnormally expressed in MI, However, its function in MI and the potential mechanism are still unclear. Objective: To evaluate the functional role of A2M-AS1 in hypoxia/reoxygenation (H/R)-induced neonatal cardiomyocytes and its potential molecular mechanism. Methods: Dataset GSE66360 was obtained from GEO database for analyzing the RNA expression of A2M-AS1 and interleukin 1 receptor type 2 (IL1R2). KEGG pathway enrichment analysis of the genes that co-expressed with A2M-AS1 was performed. Human neonatal cardiomyocytes were subjected to H/R to construct in vitro models. QRT-PCR and Western blot were adopted to test the levels of mRNA and protein. The viability and apoptosis of cardiomyocytes were tested by CCK-8 and flow cytometry assays, respectively. Results: The expression of A2M-AS1 was notably downregulated in H/R-treated cardiomyocytes. Overexpression of A2M-AS1 can notably enhance the cell viability of H/R-damaged cardiomyocytes, whereas knockdown of A2M-AS1 showed the opposite outcomes. Besides, a negative correlation was showed between A2M-AS1 and IL1R2 expression. In H/R-treated cardiomyocytes, overexpression of IL1R2 weakened the promoting proliferation and anti-apoptosis effects caused by overexpressing A2M-AS1, however, IL1R2-knockdown abolished the anti-proliferation and pro-apoptosis effects caused by silencing A2M-AS1. Conclusion: This study demonstrates the potential regulatory role of A2M-AS1/ IL1R2 axis in cardiomyocytes suffered from H/R, and provides insight into the protection of MI/RI.

Combined transcriptomic and proteomic analysis of flubendiamide resistance in Plutella xylostella

Li Jing‐Jing,Jin Ming‐Hui,Wang Nian‐Meng,Yu Qi‐Tong,Shang Ze‐Yu,Xue Chao‐Bin 한국곤충학회 2020 Entomological Research Vol.50 No.10

Diamondback moth (DBM), Plutella xylostella, is an important pest of crucifers worldwide. The extensive use of diamide insecticides has led to DBM resistance in the world, and this presents a serious threat to vegetable production. In the present study, transcriptomic and proteomic analyses were combined to investigate the potential flubendiamide‐resistance mechanism in DBM. The lab‐selected (Rh) and field‐collected (Rb) flubendiamide‐resistant lines of P. xylostella with resistance ratios of 1889.92‐fold and 1250.97‐fold, respectively, were used, as well as a lab‐reared flubendiamide‐susceptible line (S). Compared with the S group, the transcriptomic analysis revealed 151 upregulated and 287 downregulated gene messengers in the Rh group and 432 upregulated and 565 downregulated gene messengers in the Rb group. The most frequently enriched pathways of differentially expressed genes (DEGs) were mainly involved in metabolic pathways. Metabolism related genes, including two P450, two ABC transporters, and three trypsins, were upregulated in the Rh line. Additionally, some P450 genes, trypsin, juvenile hormone (JH), and mucin genes were also upregulated in the Rb line. In proteomic analysis comparisons with the S group, there were 78 upregulated and 90 downregulated proteins in the Rh group and 221 upregulated and 155 downregulated proteins in the Rb group. Further analyses found that three CYP and 11 CYP proteins were over‐expressed in Rh and Rb lines, respectively. Four glutathione S‐transferase (GST) and four UGTs were over‐expressed in Rb line. So, we deduced that the detoxification metabolism may be the main mechanism of flubendiamide resistance in P. xylostella.

Efficient extraction and separation of vanadium and chromium in high chromium vanadium slag by sodium salt roasting-(NH4)2SO4 leaching

Jing Wen,Tao Jiang,Yingzhe Xu,Jing Cao,Xiangxin Xue 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.71 No.-

A novelty process based on sodium salt roasting-(NH4)2SO4 leaching was proposed to extract vanadiumand chromium in high chromium vanadium slag (HCVS). V2O5 and Cr2O3 was then prepared. The effectsof roasting and leaching conditions on vanadium and chromium extraction behavior were studiedsystematically and completely. Vanadium precipitation conditions and chromium reduction conditionswere optimized further. 94.6% vanadium and 96.5% chromium were extracted when HCVS and Na2CO3were mixed in the molar ratio of n(Na2CO3)/n(V2O3 + Cr2O3) of 2.5, then leached in 30 g/L (NH4)2SO4solution. 94.8% vanadium was precipitated as ammonium polyvanadate (APV) just by adjusting theleaching liquid pH at 4.5, almost all chromium was remained in liquid, achieving the efficient separationof vanadium and chromium. Chromium was then recovered by reduction and precipitation. More than99% chromium was reduced when Na2S2O5 was added in m(Na2S2O5)/m[Cr(VI)] above 3. By roasting thedeposits of vanadium and chromium respectively, 91.49% V2O5 and 89.89% Cr2O3 were obtained. Thesupernatant after vanadium and chromium extraction containing NH4+ could be recycled as the newleaching medium with some new (NH4)2SO4 added, which greatly reduced the discharge of ammonianitrogenwastewater and made the whole process more environmentally friendly.

Soil Ph and Ammonium Concentrations Affect Acid Soil Microbial Nitrification Process

Jing Che,Xue Qiang Zhao,Xue Zhou,Zhong Jun Jia,Ren Fang Shen 한국토양비료학회 2014 한국토양비료학회 학술발표회 초록집 Vol.2014 No.6

Prognostic Role of Circulating Tumor Cells in Patients with Pancreatic Cancer: a Meta-analysis

Ma, Xue-Lei,Li, Yan-Yan,Zhang, Jing,Huang, Jing-Wen,Jia, Hong-Yuan,Liu, Lei,Li, Ping Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.15

Background: Isolation and characterization of circulating tumor cells (CTCs) in patients suffering from a variety of different cancers have become hot biomarker topics. In this study, we evaluated the prognostic value of CTCs in pancreatic cancer. Materials and Methods: Initial literature was identified using Medline and EMBASE. The primary data were hazard ratios (HRs) with 95% confidence intervals (CIs) of survival outcomes, including overall survival (OS) and progression free survival/recurrence free survival (PFS/RFS). Results: A total of 9 eligible studies were included in this meta-analysis, published between 2002 and 2013. The estimated pooled HR and 95%CI for OS for all studies was 1.64 (95%CI 1.39-1.94, p<0.00001) and the pooled HR and 95%CI for RFS/DFS was 2.36 (95%CI 1.41-3.96, p<0.00001). The HRs and 95%CIs for OS and RFS/DFS in patients before treatment were 1.93 (95%CI 1.26-2.96, p=0.003) and 1.82 (95%CI 1.22-2.72, p=0.003), respectively. In patients receiving treatment, the HRs and 95%CI for OS and RFS/DFS were 1.37 (95%CI 1.00-1.86, p=0.05) and 1.89 (95%CI 1.01-3.51, p=0.05), respectively. Moreover, the pooled HR and 95%CI for OS in the post-treatment group was 2.20 (95%CI 0.80-6.02, p=0.13) and the pooled HR for RFS/DFS was 8.36 (95%CI 3.22-21.67, p<0.0001). Conclusions: The meta-analysis provided strong evidence supporting the proposition that CTCs detected in peripheral blood have a fine predictive role in pancreatic patients especially on the time point of post-treatment.

Diagnostic Accuracy of 18F-FDG-PET in Patients with Testicular Cancer: a Meta-analysis

Zhao, Jing-Yi,Ma, Xue-Lei,Li, Yan-Yan,Zhang, Bing-Lan,Li, Min-Min,Ma, Xue-Lei,Liu, Lei Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.8

Objective: Fluorine-18-fluorodeoxyglucose positron emission tomography (18F-FDG-PET) is a new technique for identifying different malignant tumors using different uptake values between tumor cells and normal tissues. Here we assessed the diagnostic accuracy of 18F-FDG-PET in patients with testicular cancer by pooling data of existing trials in a meta-analysis. Methods: PubMed/MEDLINE, Embase and Cochrane Central Trials databases were searched and studies published in English relating to the diagnostic value of FDG-PET for testicular cancer were collected. The summary receiver operating characteristic (SROC) curve was used to examine the FDG-PET accuracy. Results: A total of 16 studies which included 957 examinations in 807 patients (median age, 31.1 years) were analyzed. A meta-analysis was performed to combine the sensitivity and specificity and their 95% confidence intervals (CIs), from diagnostic odds ratio (DOR), positive likelihood ratios (PLR), negative likelihood ratio (NLR). SROC were derived to demonstrate the diagnostic accuracy of FDG-PET for testicular cancer. The pooled sensitivity and specificity were 0.75 (95% confidence interval (CI), 0.70-0.80) and 0.87 (95% CI, 0.84-0.89), respectively. The pooled DOR was 35.6 (95% CI, 12.9-98.3). The area under the curve (AUC) was 0.88. The pooled PLR and pooled NLR were 7.80 (95% CI, 3.73-16.3) and 0.31 (95% CI, 0.23-0.43), respectively. Conclusion: In patients with testicular cancer, 18F-FDG-PET demonstrated a high SROC area, and could be a potentially useful tool if combined with other imaging methods such as MRI and CT. Nevertheless, the literature focusing on the use of 18F-FDG-PET in this setting still remains limited.

The Analysis on the Development of South Korean Cruise Tourism of Chinese Tourist Market

Sun, Jing,Jin, Xue,Zheng, Xiaoyi 한중경제문화학회 2015 한중경제문화연구 Vol.4 No.-

As the World Tourism Organization points out that cruise tourism is the best tour product for people to enjoy the diverse experience within the shortest terms. With the constant development of cruise tourism through the years, it has gained the attention of the South Korea. This study analyzes the general information of the cruise ports in South Korea, Chinese cruise tourists to South Korea. To solve problems in the development of cruise tourism of South Korea, this study brings forward to the countermeasures including the developing specialized cruise ports, increasing variety of tour products and strengthening train programs.

Bronchopulmonary Infection of Lophomonas blattarum

Jian Xue,Ying-Li Li,Xue-Mei Yu,Dai-Kun Li,Ming-Fang Liu,Jing-Fu Qiu,Jian-Jiang Xue 대한기생충학열대의학회 2014 The Korean Journal of Parasitology Vol.52 No.5

Elastography for Breast Cancer Diagnosis: a Useful Tool for Small and BI-RADS 4 Lesions

Liu, Xue-Jing,Zhu, Ying,Liu, Pei-Fang,Xu, Yi-Lin Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.24

The present study aimed at evaluating and comparing the diagnostic performance of B-mode ultrasound (US), elastography score (ES), and strain ratio (SR) for the differentiation of breast lesions. This retrospective study enrolled 431 lesions from 417 in-hospital patients. All patients were examined with both conventional ultrasound and elastography. Two experienced radiologists reviewed ultrasound and elasticity images. The histopathologic result obtained from ultrasound-guided core biopsy or operation excisions were used as the reference standard. Pathologic examination revealed 276 malignant lesions (64%) and 155 benign lesions (36%). A cut-off point of 4.15 (area under the curve, 0.891) allowed significant differentiation of malignant and benign lesions. ROC (receiver-operating characteristic) curves showed a higher value for combination of B-mode ultrasound and elastography for the diagnosis of breast lesions. Conventional ultrasound combined elastography showed high sensitivity, specificity, and accuracy for group II lesions (10mm<lesion diameter ${\leq}20mm$). Elastography combined with conventional ultrasound show high specificity and accuracy for differentiation of benign and malignant breast lesions. Elastography is particularly important for the diagnosis of BI-RADS 4 and small breast lesions.

축산지역의 오염부하량 산정 및 배출특성에 관한 연구

설경휘 ( Jing-hui Xue ),윤춘경 ( Chun-gyeong Yoon ),류제하 ( Je-ha Ryu ),최재호 ( Jae-ho Choi ),조문수 ( Moon-soo Cho ) 한국농공학회 2013 한국농공학회 학술대회초록집 Vol.2013 No.-

축산분뇨는 고농도의 유기물과 영양염류를 함유하고 있어 자원화에 유리한 점을 지니고 있으나, 미처리된 상태로 수계에 유입되면 하천의 수질악화 및 호소의 부영양화를 유발하여 용수의 질적 저하를 초래한다. 뿐만 아니라 축산분뇨가 무단 폐기될 경우 토양오염 및 지하수의 오염을 유발하여 악취 및 해충의 번식으로 인한 전반적인 생활환경을 악화시키게 되며 사회환경 및 경제에 악영향을 초래하게 된다. 따라서, 본 연구에서는 축산지역에서 배출되는 오염부하량의 배출특성 및 배출량을 파악하고자 축사시설이 많이 분포하는 유역을 선정하여, 해당 유역의 축종별 발생량 및 농도 등의 배출 특성을 조사하고 발생/배출 부하량을 산정하여 수질오염총량관리에 필요한 기초자료를 제시하고자 한다. 대상유역은 경기도 여주군의 간매천 유역 일대이며 축산계 배출부하량을 산정하기 위해 축산시설의 퇴ㆍ액비 배출량, 배출농도 및 슬러지 발생량 등의 자료를 조사하였다. 또한 하천의 하류부의 각 측정데이터를 이용하여 유달율을 산정하였으며 이를 이용하여 유달부하량을 산정하였다. 산정결과 간매천 유역에서 총배출부하량은 BOD 192.6kg/Day, T-N 113.8kg/Day, T-P가 12.1kg/Day로 조사되었다. 이 중, 축산지역에서 배출되는 부하량은 BOD 78.5kg/Day, T-N 15.0kg/Day로 전체의 41%(BOD기준)를 차지하였으며 T-P의 경우에는 매우 미비한 것으로 나타났다. 유달부하량의 경우는 BOD 4.68kg/Day, T-N 15.0kg/Day, T-P가 0.7kg/Day를 하고 유달율의 경우는 BOD 2.43%, T-N 13.19%, T-P 5.75%로 산정하였다.