Evaluation of Different Inoculation Methods for Screening of Sclerotinia rot and Phytophthora blight in Perilla Germplasm

Tania Afroz,이호선,전영아,성정숙,이주희,Awraris Derbie Assefa,노재정,황애진,허온숙,노나영,이재은,이명철 한국작물학회 2019 Journal of crop science and biotechnology Vol.22 No.2

Sclerotinia rot and Phytophthora blight are devastating diseases of perilla that can cause huge loss of perilla production. Screening disease resistant genetic resources is necessary to develop disease-resistant cultivars and conduct related research. The objective of this study was to find suitable inoculation methods for screening of Sclerotinia rot and Phytophthora blight in perilla germplasm. Three different inoculation methods [i.e. detached leaf (agar plug placed on start point of vein, 1/3 point of leaf vein and front side, back side), stem tip, and soil drenching] with three-growth stages (two, four & six-leaf stage) at two different temperatures (20 & 25oC) were assayed for their effectiveness for screening using IT 229019 and Sang-yeop perilla germplasm. Based on disease lesion, 20oC in two-leaf stage with detached leaf method (start point of vein) was found to be an efficient method for screening of Sclerotinia rot whereas 25oC in two-leaf stage with stem tip was an effective method for screening of Phytophthora blight in perilla germplasm. However, stem tip method was laborious as it required more time. Therefore, detached leaf method (start point of vein and back side) was a simple and rapid method that could be preferably used for screening of Sclerotinia rot and Phytophthora blight in perilla germplasm.

Evaluation of Bioassay Methods to Assess Bacterial Soft Rot Resistance in Radish Cultivars

Tania Afroz(타니아 아프로즈),Onsook Hur(허온숙),Nayoung Ro(노나영),Jae-eun Lee(이재은),Aejin Hwang(황애진),Bichsaem Kim(김빛샘),Awraris Derbie Assefa(아와리스 데비 아세파),Ju Hee Rhee(이주희),Jung Sook Sung(성정숙),Ho-sun Lee(이호선),Bum-So 한국생명과학회 2021 생명과학회지 Vol.31 No.7

세균성 무름병 균[Pectobacterium carotovorum subsp. carotovorum (Pcc)]에 의해서 일어나는 무름병은 아시아 국가에서 재배되는 무에 있어서 심각한 질병 중의 하나로 알려져 있다. 이번 연구의 목적은 상업적으로 시판되는 무 품종의 세균성 무름병의 저항성에 대한 효율적인 생물검정법을 확립하고자 하였다. 첫째, 무 품종에 대해 세균성 무름병의 효율적인 생물검정 방법을 조사하였다. 6개의 무 품종을 다양한 조건[두 가지 온도(25℃와 30℃), 3가지 접종방법(관주, 분무, 침지), 두 발생단계(2와 4잎 단계)]으로 조사하였다. 연구 결과는 무름병균 1×106 cfu/ml 농도를 분무한 4잎 단계와 30℃에서 배양한 생물검정 방법이 무 품종에 대해 가장 효율적인 방법임을 알 수 있었다. 둘째, 41개의 무 품종에 5가지 세균(KACC 10225, KACC 10343, KACC 10421, KACC 10458, KACC 13953)을 접종하여 저항성을 조사하였다. KACC 10421가 세균성 무름병의 감수성 및 저항성 질병 정도를 가장 잘 나타냈다. 41개의 무 품종 중 13개는 무름병 균에 대해 중도 저항성을 나타냈고 28개는 감수성을 나타냈다. 이 연구에서 중도저항성 무 품종은 세균성 무름병 저항성 육종을 위한 저항성 자원으로 활용 가능하고 육종가, 농민, 연구자, 최종 소비자에 의해서 다양한 목적을 위해 사용 가능할 것으로 사료된다. Bacterial soft rot, caused by Pectobacterium carotovorum subsp. carotovorum (Pcc), is one of the destructive diseases of radish (Raphanus sativus) in Asian countries. The objective of this study was to establish an efficient bioassay method for the evaluation of bacterial soft rot resistance in commercial radish cultivars. First, an efficient bioassay method for examining resistance to bacterial soft rot in commercial radish cultivars was investigated. Six commercial radish cultivars were tested under various conditions: two temperatures (25℃ and 30℃), three inoculations methods (drenching, spraying, and root dipping), and two growth stages (two- and four-leaf stages). The results suggested that spraying with 1×106 cfu/ml of bacterial inoculums during the four-leaf stage and incubating at 30℃ could be the most efficient screening method for bacterial soft rot resistance in commercial radish cultivars. Second, we investigated the degree of resistance of 41 commercial radish cultivars to five Pcc isolates, namely KACC 10225, KACC 10343, KACC 10421, KACC 10458, and KACC 13953. KACC 10421 had the strongest susceptibility in terms of moderately resistant disease response to bacterial soft rot. Out of the 41 radish cultivars, 13 were moderately resistant to this pathogen, whereas 28 were susceptible. The moderately resistant radish cultivars in this investigation could serve as resistance donors in the breeding of soft rot resistance or could be used to determine varietal improvement for direct use by breeders, scientists, farmers, researchers, and end customers.

Lamang tapai: the ancient Malay food in Minangkabau tradition

Tania Yovani 한국식품연구원 2019 Journal of Ethnic Foods Vol.6 No.-

Lamang tapai is a traditional food which consists of lemang and tapai that are used in various traditional ceremonies of Minangkabau people, mainly in West Sumatra. However, lemang and tapai are also known as traditional foods of other tribes or regions in Southeast Asia, and their cooking method is still very ancient and depends on the state of nature. These two points show that lemang and tapai can be used as historical evidence for ancient human life in Southeast Asia, which is Proto-Malay and Deutero-Malay. Minang people believe that cooking technique of lemang was first introduced by Sheikh Burhanuddin. Lemang is incomplete if it is not eaten together with tapai, so they are likened to a man and a woman by Minang people. Lemang itself describes the togetherness of Minang people because its making process is always done together. There are several taboos that must be obeyed in making lemang and tapai. Lamang tapai are also used as gifts when visiting other people’s homes, for example, when visiting in-laws or manjapuik marapulai ceremony. However, there is no symbolic meaning behind the obligatory existence of lamang tapai at traditional ceremonies. On the other hand, lemang and tapai are famous for their unique taste produced by the chemical components in their ingredients. In this article, the origin of lemang and tapai, the philosophy and presentation of lemang in the traditions of the Minangkabau people, and the flavor features of lemang and tapai from a scientific perspective are discussed.

In Vitro Fungicide Sensitivity of Alternaria spp. Isolated from Cruciferous Crops to Commonly Used Fungicides

Tania Afroz,Md. Aktaruzzaman,Byung Sup Kim 한국농약과학회 2018 한국농약과학회 학술발표대회 논문집 Vol.2018 No.4

Remote sensing based forest canopy opening and their spatial representation

Tania Fernández Vargas,Trejo Vázquez Irma,Aguirre Gómez Raúl 한국산림과학회 2021 Forest Science And Technology Vol.17 No.4

The use of remote sensing in natural resource management is an easily accessible input for obtaining detailed information on the ground and landscape. There is a wide range of procedures to analyze the forest canopy through satellite images. The purpose of this work is to obtain a map of forest opening with remote sensing by relating several vegetation indices, Kauth-Thomas transformation and texture filters, to a Landsat 8OLI image. A factor analysis was made to evaluate the contribution of these variable to identify the opening of the forest cover, yielding a r2 ¼ 76%. The results show that the Modified Soil Adjusted Vegetation Index (MSAVI), Soil Adjusted Vegetation Index (SAVI), and brightness factor have the best correlation (0.225–0.216 component coefficient). The resulting model was reclassified into five categories of forest opening and associated with land use data from the National Institute of Statistics and Geography (INEGI-M exico). Thus, 95% of human settlements have a canopy opening between medium and very high, the crops areas 72%, and the low deciduous forest with secondary shrub vegetation 100% of the opening. Coniferous and mixed forests have a low to very low canopy opening 46% and 55%, respectively of their surface. The forests with secondary vegetation, both shrub and arboreal, present greater openness than the same forests in the primary state. Verification of the spatial representation data of canopy opening was made by comparing 94 hemispheric photographs with 94 sites located in open areas obtaining an r ¼ 0.57. This work offers a simple and straightforward methodology, easily replicable in different types of vegetation using free satellite imagery. Hence, it is a helpful tool for decision-makers when considering the general status of conservation of forest systems and their spatial distribution.

Considering conceptual orientations of coach education research

Tania Cassidy,Clifford Mallett,Richard Tinning 한국코칭능력개발원 2008 International Journal of Coaching Science Vol.2 No.2

A Series of Novel Esters of Capsaicin Analogues Catalyzed by Candida antarctica Lipases

Tania Diaz-Vidal,Luis Carlos Rosales-Rivera,Juan C. Mateos-Díaz,Jorge A. Rodríguez 한국생물공학회 2020 Biotechnology and Bioprocess Engineering Vol.25 No.1

Capsaicin analogues are typically synthetized by condensation of the amide group of vanillylamine with a fatty acid derivative. The enzyme of choice to perform this reaction is Candida antarctica lipase B; however, this enzyme is unable to react with the phenolic substituents of the vanillyl ring. So far, this can only be achieved chemically, resulting in capsaicin esters with higher lipophilicity and less irritation than the parent compound. In need of searching for new capsaicin derivatives, we investigated the transesterification of the phenolic OH group of capsaicin and several capsaicin analogues by Candida antarctica lipase A (CALA) with vinyl esters. Capsaicin esters were successfully synthetized with a reaction yield of 80.6% and 57.5% with vinyl butyrate and vinyl laurate, respectively. When the reactions were performed with capsaicin analogues of different acyl chain lengths, CALA exhibited a noticeable transesterification preference for medium-length capsaicin analogues. In an attempt to explore the catalytic limits of CALA, we performed the transesterification of capsaicin from Capsicum oleoresin. The results showed similar transesterification yields to those obtained with semi-pure capsaicin. Within our knowledge, this is the first report to achieve the synthesis of phenolic OH esters of capsaicin and capsaicin analogues by enzymatic means.

Numerical and laboratory investigations of electrical resistance tomography for environmental monitoring

Tania Dhu,Graham Heinson 한국지구물리탐사학회 2004 지구물리와 물리탐사 Vol.7 No.1

New Insights on the Mechanisms Affecting Fertility in Men with Non-Seminoma Testicular Cancer before Cancer Therapy

Tania R. Dias,Ashok Agarwal,Peter N. Pushparaj,Gulfam Ahmad,Rakesh Sharma 대한남성과학회 2020 The World Journal of Men's Health Vol.38 No.2

Purpose: Patients with non-seminoma testicular cancer (NSTC) cancer can be subfertile or infertile, and present reduced sperm quality, but the underlying mechanisms are unknown. The aim of this study was to compare the sperm proteome of patients with NSTC, who cryopreserved their sperm before starting cancer treatment, with that from healthy fertile men.Materials and Methods: Semen volume, sperm motility and sperm concentration were evaluated before the cryopreservation of samples from patients with NSTC (n=15) and the control group (n=15). Sperm proteomic analysis was performed by liquid chromatography-tandem mass spectrometry and the differentially expressed proteins (DEPs) between the two groups were identified using bioinformatic tools.Results: A total of 189 DEPs was identified in the dataset, from which five DEPs related to sperm function and fertilization were selected for validation by Western blot. We were able to validate the underexpression of the mitochondrial complex subunits NADH:Ubiquinone Oxidoreductase Core Subunit S1 (NDUFS1) and ubiquinol-cytochrome C reductase core protein 2 (UQCRC2), as well as the underexpression of the testis-specific sodium/potassium-transporting ATPase subunit alpha-4 (ATP1A4) in the NSTC group. Conclusions: Our results indicate that sperm mitochondrial dysfunction may explain the observed decrease in sperm concentration, total sperm count and total motile count in NSTC patients. The identified DEPs may serve as potential biomarkers for the pathophysiology of subfertility/infertility in patients with NSTC. Our study also associates the reduced fertilizing ability of NSTC patients with the dysregulation of important sperm molecular mechanisms.

NITROUS OXIDE BIOFILTRATION USING GOAT-MANURE BASED PELLETIZED COMPOST AS FILTER MEDIA

Tania Surya Utami,Irwan Warta,M. Nasikin,Heri Hermansyah 한국생물공학회 2010 한국생물공학회 학술대회 Vol.2010 No.10