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Pranav R. Buch,Pradeep Sarkate,Gowri K. Uggini,Isha Desai,Suresh Balakrishnan 한국조직공학과 재생의학회 2017 조직공학과 재생의학 Vol.14 No.2
Epimorphic regeneration in vertebrates involves the restoration of lost tissue or organs through the formation of a regeneration blastema and occurs through a complex interaction of a number of molecular signaling pathways. Of the many effectors of successful tail regeneration in the lizard Hemidactylus flaviviridis, one crucial pathway is the cyclooxygenase-2 (COX-2) mediated PGE2 signaling pathway. The current study was aimed at understanding whether COX-2 signaling plays any role in the expression of Wnt/b-Catenin signaling components during regenerative outgrowth in H. flaviviridis. Etoricoxib—selective inhibitor of the inducible isoform of COX-2—was administered to lizards orally. We tested the expression of b-Catenin during wound epidermis and blastema stages in the regenerating tail and found a reduction in its expression in response to drug treatment. Further, it was observed that the expression of canonical Wnt ligands was greatly altered due to COX-2 inhibition. Our results provide evidence of a cross-talk between the COX-2 induced PGE2 pathway and Wnt/b-Catenin signaling in the regenerating lizard tail. An understanding of the interaction among various signaling pathways will help elucidate the mechanism underlying epimorphosis in lizards, the only amniotes capable of appendage regeneration.
Pranav D. Ingole,Jignesh G. Rajguru,Nilima J. Budhraja,Ramakrishna S. Shenoi,Jui S. Karmarkar,Rahul N. Dahake 대한구강악안면외과학회 2022 대한구강악안면외과학회지 Vol.48 No.2
Various techniques are well documented to obtain anatomic reduction, such as reduction forceps, manual reduction, or a combination of these methods. However, these techniques have inherent drawbacks. We propose a new intra-operative technique for anatomic reduction using screw-wire traction for open reduction and internal fixation of mandibular fractures.
Pranav H. Nakhate,Chandrakanth R. Gadipelly,Nandkumar T. Joshi,Kumudini V. Marathe 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.69 No.-
Present study investigated the development and use of copper-doped zinc oxide as catalysts to facilitate degradation of real textile wastewater by ozonation. Effect of various operating parameters like catalyst dosage, pH, and ozone dosage was examined. The Cu-doped ZnO removes 89% of COD within 30 min of reaction whereas tertiary-butyl alcohol inhibited COD reduction at 10 mM concentration under the optimised conditions, indicating that catalytic ozonation follows hydroxyl (OH) radical method for COD reduction. MATLAB ODE45 function was used to draw kinetic plots where catalytic ozonation shows six times higher rate constant. Catalytic ozonation has been successfully implemented at pilot-scale.
Pranav SASIDHARAN,Hyunjin KIM,Okkyoung CHOI,Byoung-In SANG 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Chain elongation (CE) technology, biological conversion to medium chain carboxylic acids (MCCAs) from short chain organic compounds, has gained more attraction recently due to the production of value-added products from organic waste. In the CE process, it has always been a bottleneck that the low selectivity of MCCAs compared with short chain acetate and/or butyrate. In this study cell growth and metabolite composition of Caproiciproducens galactitolivorans cultivated under high hydrogen (H₂) partial pressure were compared to those obtained from the control. The supplement of hydrogen led to a significant improvement in the final metabolite production ratio (n-caproate/acetate [mol C/㏖ C]) compared to the control (from 1.90 to 2.78). Hydrogen enhanced the reducing power for n-caproate production by altering the electron flow from ferredoxin to NADH, resulting in reduced carbon flow towards acetate production. Cultivation of Caproiciproducens galactitolivorans using hydrogen as an electron donor could be considered as a feasible alternative to improve the n-caproate production.
Pranav Chauhan,Soubhagya Ranjan Pradhan,Annada Das,Pramod Kumar Nanda,Samiran Bandyopadhyay,Arun K. Das 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.2
Objective: Terminalia arjuna plant, specially its leaves, bark, and roots, are widely used in traditional herbal medicine due to presence of bioactive components and being a rich source of natural antioxidants. But its fruit has not been used for any such purposes despite its potential to retard oxidation. Hence, the antioxidant potential of Arjuna fruit extract (AFE) in retarding lipid and protein oxidation of raw ground pork was evaluated during refrigerated storage for 9 days. Methods: The AFEs were prepared using different solvents viz. ethanol (EH), water, ethanol: water (60:40) and methanol:hot water (60:40). The AFEs were analysed for total phenolic content (TPC), 2, 2-diphenyl-1-picrylhydrazyl radical scavenging activity and reducing power. Water extract (WE) and ethanol-water extract (EH-WE) were selected and incorporated at 1.0% into freshly minced pork meat and compared with a synthetic antioxidant, in retarding lipid and protein oxidation during storage. Results: The TPC in AFEs using different solvents ranged from 11.04 to 16.53 mg gallic acid equivalents/g and extracts exhibited appreciable scavenging activity ranging from 50.02% to 58.62%. Arjuna extracts significantly (p<0.05) improved the colour score of meat samples by reducing the formation of metmyoglobin during storage. Both the AFEs (WE and EH-WE) significantly (p<0.05) lowered the thiobarbituric acid reactive substances value, peroxide formation and formation of protein carbonyls in raw pork than control sample during storage. Upon sensory evaluation of all samples, it was found that AFE treatment could prolong the storage period of meat samples, without influencing the colour and odour score, up to 6 days. Conclusion: AFEs used at 1% improved the oxidative stability, colour and odour score and prolonged the refrigerated shelf life of ground pork up 6 days. Therefore, AFE could be explored as an alternative natural antioxidant in retarding lipid and protein oxidation in meat products.