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Pranav SASIDHARAN,Hyunjin KIM,Okkyoung CHOI,Byoung-In SANG 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Chain elongation (CE) technology, biological conversion to medium chain carboxylic acids (MCCAs) from short chain organic compounds, has gained more attraction recently due to the production of value-added products from organic waste. In the CE process, it has always been a bottleneck that the low selectivity of MCCAs compared with short chain acetate and/or butyrate. In this study cell growth and metabolite composition of Caproiciproducens galactitolivorans cultivated under high hydrogen (H₂) partial pressure were compared to those obtained from the control. The supplement of hydrogen led to a significant improvement in the final metabolite production ratio (n-caproate/acetate [mol C/㏖ C]) compared to the control (from 1.90 to 2.78). Hydrogen enhanced the reducing power for n-caproate production by altering the electron flow from ferredoxin to NADH, resulting in reduced carbon flow towards acetate production. Cultivation of Caproiciproducens galactitolivorans using hydrogen as an electron donor could be considered as a feasible alternative to improve the n-caproate production.