내분비계 교란물질의 검출계를 이용한 γ-HCH의 미생물에 의한 중간대사산물에 대한 내분비계교란 활성의 평가

이행석,박주석,조은민,문명숙,太田明德,류재천 대한상하수도학회 2003 상하수도학회지 Vol.17 No.1

To develop an efficient degradation system for Endocrine disruptors (EDs), it is necessary to have a good system to evaluate rapidly and accurately endocrine-disrupting activities of suspected chemicals and their degradation products. We previously constructed a co-expression system of GAL4 DNA binding domain (DBD)-human estrogen receptorβ ligand binding domain (hERβ LBD) and Gal4p transcriptional activation domain (TAD)-co-activator SRC1 in Saccharomyces cerevisiae with a chromosome-integrated lacZ reporter gene under the control of CYC1 promoter and GAL4 binding site (Upstream Activating Sequence, UAS). Expression of this reporter gene was dependent on the presence of estrogen or endocrine disruptors in the culture medium. Furthermore, the extent of transcriptional activation by those chemicals correlated with their estrogenic activities measured by other assay systems, indicating that this assay system is efficient and reliable for measuring estrogenic activity. We applied this assay system to measure estrogenic activity of microbial degradation products of γ-hexachlorocyclohexane (γ-HCH) by Sphingomonas paucimobilis. Among the γ-HCH metabolites, 2,5-dichlorohydroquinone (2,5-DCHQ) and chlorohydroquinone (CHQ) had similar estrogenic activities to the original chemical, but hydroquinone (HQ), a metabolite at later stage, had no activity at the concentration of 10^-4M, showing the necessity of evaluation of intermediate metabolites in microbial degradation systems.

施設園藝地에서 有效硼素 含量에 대한 土壤有機物의 相對 寄與度

李在奭,최명자,정재식 대구효성가톨릭대학교 1997 연구논문집 Vol.56 No.2

우리나라 시설원예지에서 유효불소 함량에 대한 토양유기물의 상대 기여도를 구명하고자 경기, 충남, 경북, 제주도에서 채취한 시설원예지 토양을 공시하여 얻은 결과는 다음과 같다. 1. 시설원예지의 유효불소 함량은 최저 0.17ppm, 최고 2.24ppm이었고, 육지토양이 0.93±0.62 ppm이었고 제주도 토양은 0.41±0.20ppm이었다. 2. 토양유기물의 함량은 최저 0.46%, 최고 15.20%이었으며, 평균함량은 육지토양이 1.93±1.21%이었고, 제주도 토양은 13.4±1.95%로 육지토양보다 높았다. 3. 표토의 유기물 함량과 유효불소함량 간에 육지토양은 고도의 유의성(r=0.6440**)있는 정상관이었으나, 제주도 토양은 유의성(r=0.5024)이 없었다. 4. 토양유기물이 1단위 증가함에 따라 붕소의 함량은 육지토양이 1.1596 ppm, 제주도 토양은 0.0392 ppm씩 통계적으로 증가하는 경향이었다. 5. 유효붕소 함량에 대한 토양유기물의 기여도는 유기물의 함량이 적은 육지토양에서 높았고, 유기물의 함량이 많은 제주도 토양은 낮았다 This study was conducted to identify the contribution ratio of soil organic matter for an available boron content in plastic film house soils which were obtained from the soils of Gyeong-gi, Chung Nam, Gyeong Buk and Jeju areas. The results are summarized as follows; 1. Available boron content in plastic film house soils was from 0.07 ppm to 2.24 ppm, average content was 0.93±0.62 ppm(inland soils) and 0.41±0.20ppm(Jeju soil), respectively. 2. The content of organic matter was from 0.46% to 15.20% average content was 1.93±1.21%(inland soil) and 13.4±1.95%(Jeju soil), respectively. 3. The inland soils between organic matter content and available boron content showed a highly positive correlation coefficiency (r=0.6440**), while Jeju soil was positive correlation coefficiency (r=0.5024). 4. The content of available horon, as increasing 1 unit of soil organic matter, was increased as 1.1596 ppm in inland soils, while it was increased as 0.0392 ppm in Jeju soil. 5. The contribution ratio of soil organic matter for avaiable boron content was high in inland soils, which have low content of ofganic matter, while it was low in Jeju soil containing a high content of organic matter.

양성 갑상선 결절에서 티록신 억제 요법의 효과

윤석기,김철희,김영선,변동원,서교일,유명희 대한내분비학회 2000 Endocrinology and metabolism Vol.15 No.4·5

무 種子의 發芽와 生育에 미치는 産性비의 影響

이재석,최명자,정재식,이영선 대구효성가톨릭대학교 응용과학연구소 1997 응용과학연구논문집 Vol.6 No.1

황산과 질산을 3:1용량비율(V/V)로 혼합한 인공산성비(pH 2.0∼pH 4.0)처리가 무(Raphanus sativus L.) 종자의 발아, 유근과 배축의 생장, 세근, 근모의 발생 등에 미치는 영향을 조사하여 얻은 결과는 다음과 같다. 1. 종자의 발아율과 발아세는 대조구(pH 6.3)에 비하여 pH 3.0과 pH 4.0은 비슷한 경향이었으나, pH 2.0은 발아가 지연되고 불균일하였으며, pH 1.0은 전혀 발아가 되지 않았다. 2. 유근과 배축의 생장은 대조구(pH 6.3)에 비하여 pH 3.0과 pH 4.0은 차이가 없었으나, pH 2.0은 극히 불량하였으며 산성비는 배축보다 유근의 신장을 더 저해시켰다. 3. pH 2.0의 산성비는 치상후 3일째부터 유근과 배축이 서서히 굽어지고 세근과 근모가 전혀 발생되지 않았다. 4. pH 2.0의 산성비는 6일째부터 유근이 누렇게 되고 자엽이 연녹색으로 변색되었으며 엽록소가 거의 생성되지 않았다. 5. 산성이 강한 용액일수록 자엽, 배축, 유근 등의 조직이 일찍 물러지고 괴사되는 현상이 나타났다. The effects of acid rain on the germination, and growth of radical and hypocotyl of radish are studied. The inhibition effect of simulated acid rain of pH3.0 and pH4.0 on seed germination was not significant as compared to control treatment(pH6.3), while that pH2.0 reduced both germination rate and germination speed significantly. No seed germination was observed in the group treated with pH1.0. Growth of radical and hypocotyl of the seedlings treated with pH3.0 and pH4.0 was not inhibited when compared to that of control group(pH 6.3). Growth of radical and hypocotyl of the seedlings treated with pH2.0 was severly inhibited which resulted in gradual abnormal hypocotyl bending. Forthmore, no fine roots and root hairs were formed on these seedlings. Simulated acid rain of pH2.0 cused radicle chlorosis, and decolorization of cotylendons to pale green which might has been due to lack of chlorophyll synthesis.

항진균성 항생물질을 생산하는 Streptoverticillium sp. NA-4083의 분리 및 동정

임대석,윤상균,이명섭,윤원호,김창한 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.6

전국 각지의 토양에서 441균주의 방선균을 분리한 결과 Trichophyton mentagrophytes, Trichophyton rubrum, Nanniza otae 및 Pyricularia oryzae 등의 진균에 광범위한 항진균활성을 나타내는 방선균 NA-4803을 선발하여 그 균주의 동정을 실시하였다. 분리균주 NA-4803은 Gram 양성세균 4균주, Gram 음성세균 2균주, 효모 2균주, 곰팡이 5균주, 조류 1균주에 대해 약한 항균활성을 보이는 반면, 곰팡이 Trichophyton mentagrophytes, Trichophyton rubrum, Nanniza otae 및 Pyricularia oryzae에 대해서는 비교적 강한 항진균활성을 나타내었다. 분리균주 NA-4803은 생리적 특성, 항균활성, lectithinase 및 lipolytic 활성, 각종 유기물의 분해력, 항생물질에 대한 저항성, 온도, pH 및 화학저해제의 첨가에 따른 생육, 질소원의 이용성 및 탄소원의 이용성 등에 있어서 cluster group F에 속하며 Cluster No. 58에 해당하는 Streptoverticillium blastmyceticum과 가장 유사하게 비교·동정되었으나 nitrate 환원성의 양성반응, L-tyrosine 분해력의 음성반응 및 cephaloridine에 대한 저항성에 음성반응을 보였고, 탄소원 중 L-rhamnose와 inulin을 이용하는 것으로 보아 Streptoverticillium blastmyceticum과 다른 특성을 나타내었다. 따라서 분리균주 NA-4803은 Streptoverticillium sp. NA-4803으로 동정되었다. The aim of the present research program was to develop a strain of actinomycetes producing antifungal substance. Soil samples were collected from various sites in Korea and a number of actinomycetes were isolated from the soil samples by applying selective agar for actinomycetes. Among over 440 isolates, a strain (NA-4803) producing antifungal substance against Trichophyton spp. Nannizzia otae and Pyricularia oryzae was selected. The strain NA-4803 was identified as strain similar to Streptoverticillium blastmyceticum with respect to morphological and physiological characteristics, lecithinase and lipolytic activity, degradation of organic compounds, resistance to antibiotics and utilization of carbon and nitrogen sources. But it showed some differences such as positive reaction of nitrate reduction, negative reaction of L-tyrosine degradation, resistance to cephaloridine, and utilization of I rhamnose and inulin. The strain NA-4803 was named as Streptoverticillium sp. NA-4803.

造景樹木의 葉內 硼素含量分布

李在奭,崔明子,金貞淑 대구효성 가톨릭대학교 1994 연구논문집 Vol.48 No.1

The distribution of boron on 98 different species of woody landscape plants which belonged to 38 different families were investigated. The soils used were Daegu Series developed out of Residiuum of Shale which is commonly found in Bongduk-dong, Daegu City and Hayang-eup, Kyungsan-gun, Kyungpllk Province. Some of the results obtained are summerized as follows : 1. Among the tested palnts, 33 species including European holly contained comparatively ow level of boron and 53 ones such as apple tree required medium level of boron for the normal growth. On the contrary, the rest of 9 species including Manshurian fullmoon maple accumulated much high level of boron for the normal growth. 2. Among the tested plants, tree of heaven contained the highest level of boron(90.4±0.15ppm) while arrow bamboo contained the lowest one(5.3±0.79ppm) 3. No significant correlation between the single leaf area and the boron content of the leaves was recognized when the area of the single leaf is less than 100㎠ but highly significant correlation(r=0.769**) between above two characters was recognized when that is over 100㎠/leaf. 4. when the tested plants were classified by the content of boron in the leaves, the rate of plants having the contents of 5∼10ppm, 10∼20ppm, 20∼30ppm, 30∼40ppm, 40∼50ppm, 50∼60ppm, and over 60ppm were 1%, 35.8%, 29.6%, 23.5%, 5.1%, 2.0%, and 3.0% respectively. 5. No significant correlation(r=0.072) between the content of boron in the soils naturally developed out of Shale and the content of boron in the leaves tested was recognized.

선형 시변 시스템의 웨이브렛 망 근사화와 가중치 학습

이영석,이인수,김명식 金烏工科大學校 1997 論文集 Vol.18 No.-

This paper discusses approximation modeling of discrete-time linear time-varying system(LTVS). The wavelet transform is considered as a tool for representing and approximating a LTVS. The joint time-frequency properties of wave analysis are appropriate for describing the LTVS. Simulation results are included to illustrate the potential application of the technique.

냉각수 유동 형태 개선에 따른 엔진 성능향상에 관한 연구

홍명석,백정석 朝鮮大學校 機械技術硏究所 2004 機械技術硏究 Vol.7 No.2

The ideal cooling system of engine and the effect followed of it contribute greatly to the engine performance. The change of engine performances depending on the improvement of engine cooling system is the main subject for this study and these experiments are carried out in the specific engine. which has same technical specifications by fitting the conventional cooling system and the split cooling system. respectively and get various experimental results like engine outputs. fuel consumption. engine cooling temperature. and surface temperature of metal. etc. Reformed split cooling system is transformed from the in-line type coolant flow. which is flowing via water jacket from the cylinder block to cylinder head into the parallel type coolant flow which is also flowing via water jacket but cylinder head & cylinder block separated and blocked each other. Through these experiments. it took effect on the improvement of engine performance by advancing the ignition timing due to the reduction of engine knocking as a result of the drop of temperature in cylinder head and also this brought various positive effects like the suppression of blow-by gas creation, improved thermal efficiency, and so on due to the reduction of piston friction loss as a result of the temperature rise of metal surface and the reduction of piston gaps as a result of the reduction of total heat capacity.

산성비가 튤립(Tulipa gesneriana L.cv.Golden Apeldoorn)의 생육에 미치는 영향 : Tulipa gesneriana L. cv. Golden Apeldoorn

이재석,최명자 대구효성가톨릭대학교 응용과학연구소 1997 응용과학연구논문집 Vol.5 No.-

노지에 생육중인 튤립(Tulipa gesneriana L cv. Golden Apeldoorn)에 인공산성비를 엽면적 확대기인 3월 25일부터 3~4일 간격으로 2.0mm강우량을 10회 토양에 관주한 후 튤립의 생육,구근의 생장 및 수량에 미치는 영향을 조사하여 얻은 결과는 다음과 같다. 1. 초장, 엽장, 엽폭,화고,화폭,및 엽면적은 pH가 낮아질수록 감소하였으며, 잎이 타원형에서 긴타원형으로 변형되었다. 2. T/R율은 대조구(pH 6.3) 0.18에 비해 pH 10처리는 0.27로서 높게 나타났으며,건물중은 pH가 낮아짐에 따라 감소하였다. 3. 제1,2,3 절간의 생장은 pH 수준별로 일정한 경향을 보이지 않았으나, 목(top)생장은 pH가 낮아짐에 따라 감소하는 경향이었다. This study was carried out to investigate the effects of simulated acid rain on growth and bulb yield of tulip (Tulipa gesneriana L. cv. Golden Apeldoorn) 1. As the pH decreased, plant height, leaf width, leaf length, flower width, flower length and dry weight were decreased. Leaf shap was changed from elliptical to oblong. 2. T/R ratio of treatment of pH 1.0 was a hight level 0.27, as very high compared with control (pH 6.3), 0.18. Dry weight/per plant was decreased as decreasing pH. 3. The growth of 1st, 2nd and 3rd inernodes was not affected by pH levels, but the top growth was decreased as decreasing pH of simulated acid rain. 4. Bulb weight, bulb weight per plant and bulb number per plant were decreased as decreasing pH of simulated acid rain. Bulb number per plant of pH 1.0 was 1.3±0.5 and dividing of bulb of pH 1.0 was little. 5. Rate of yield multiplication of pH 1.0 was revealed only 130%, as compared with control, 420%. Rate of weight multiplication and yield per 3.3㎡ were decreased as decreasing pH of simulated acid rain.

양다래 과실의 아미노산조성과 유리당의 분석

李明烈,朴貞淑,李柱烈,金忠模,洪石淳 조선대학교 기초과학연구소 1992 自然科學硏究 Vol.15 No.1

Actindia chinensis의 一般成分, 遊離糖 및 amino acid 組成狀態를 알아보기 위하여 HPLC 등으로 분석, 定量한 結果는 다음과 같다. 1. 粗蜜白質量은 2.77%, 炭水化物量은 11.97%이었다. 2. 遊離糖으로는 glucose, fructose, sucrose 및 maltose가 檢出되었으며 과육부에는 fructose, 껍질부에는 sucrose 및 maltose가 많이 함유되었다. 3. 과육질 및 껍질부에서 17種의 總 및 유리 amino acid이 검출되었는데 과육부에는 isoleucine 191.52mg/100g, glutamic acid 160.93mg/100g, 껍질부에는 g1utamic acid 203.36mg/100g, aspartic acid 168.46mg/100g으로 가장 많이 함유되어 있으며, 必須 아미노산 量은 껍질부가 528.23mg/100g으로 과육부 (350.90mg/loog)보다 약 1.5배 정도 많았다. 4. 遊離 아미노산 量은 과육부 (136.89mg/loog)가 껍질부 (120.55mg/100g)에 비해 다소 많았으며 他 과일에 비하여 histidine, arginine量이 많았다. Approximate compositions, and contents of free sugars and amino acids of edible and nonedidle portion in Kiwi fruit 〔Actindia chinensis planch〕were analyzed by HPLC. The results were as follows; The contents of protein and sugars in kiwi fruit were 2.77% and 11.97%, respectively. Glucose, fructose, sucrose and maltose were detected and fructose was rich in edible portion, and sucrose and maltose in nonedible portion. 17 kinds of total amino acids were detected in edible and nonedible portion. The contents of isoleucine(191.52mg/100g) and glutamic acid (160.93mg/100g) in edible portion, and glutamic acid (203.36mg/100g) and asparatic acid (168.48mg/100g) in nonedible portion were high. Total amounts of essential amino acids of nonedidle portion (523.23mg/100g) were rich than that of edible portion (350.90mg/100g). Free amino acids were contained 136.89mg/100g in edible portion and 120.55mg/100g in nonedible portion. Histidine and arginine were found in abundance, compared to other fruits (orange and strawbeery).