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Malg-Um Lim,Jae-Sung Ryu,Ghislain Moussavou,Ju-Taek Lee,Hyun-Ki Min,Yoon-Ju Na,Chi-Hyun Ahn,In-Sun Kim,Ju-Hyoung Lee,Ji-Su Kim,Sun-Uk Kim,Kyu-Tae Chang,Kisung Ko,Deog-Bon Koo,Young-Kug Choo 한국당과학회 2013 한국당과학회 학술대회 Vol.2013 No.1
Gangliosides, play important regulatory roles in cell adhesion, survival and immunosuppressive activity. In this study, we investigated whether gangliosides can affect to immune responses in lipopolysaccharide (LPS)-stimulated porcine kidney cell line, PK15. Cell proliferation decreased, whefreas nitro oxide (NO) increased in 5 ㎍/ml of LPS-stimulated PK15. In high-performance thin layer chromatography (HPTLC) and immunofluorsence analysis showed that gangliosides GM3, GM2, GD3, GD1a and GD1b were detected, however, expression of GM3 and GM2 decreased in LPS-stimulated PK15. Furthermore, we knock- downed UDP-glucose:ceramide glucosyltransferase (Ugcg) by short hairpin (sh) RNA, which catalyzes the initial step in glycosphingolipids biosynthesis pathway. Western blot and HPTLC analyse showed that Ugcg protein and gangliosides expression decreased in Ugcg shRNA infected PK15. When we stimulated Ugcg gene knock-downed PK15 with LPS (5 ㎍/ml), cell proliferation was decreased, however, NO release increased compared to un-infected PK15. Moreover, we investigated the expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and tumor necrosis factor-α (TNF-α), and these protein expression levels increased in LPS-stimulated Ugcg gene knock-downed PK15. These results suggest that gangliosides, specifically GM3 and GM2, may play a role in the interaction with components of the inflammatory response, and to be relevant for designing future therapeutic strategies intended to prolong xenotransplantation.
Expression of gangliosides during differentiation to endothelial cells of mouse embryonic stem cells
Malg-Um Lim,Jae-Sung Ryu,Ghislain Moussavou,Ju-Taek Lee,Hyun-Ki Mun,Yoon-Ju Na,Su-Bin Lee,In-Sun Kim,Ju-Hyoung Lee,Ji-Su Kim,Sun-Uk Kim,Kyu-Tae Chang,Young-Kug Choo 한국당과학회 2012 한국당과학회 학술대회 Vol.2012 No.1
Malg-Um Lim,Sung-Youn Heo,Mi-Ran Hwang,Yoon-Ju Na,Ji-Su Kim,Sun-Uk Kim,Kyu-Tae Chang,Young-Kug Choo 한국당과학회 2013 한국당과학회 학술대회 Vol.2013 No.1
Gangliosides are sialic acid-containing glycosphingolipids that are abundant in neurons and have a variety of functions in developing and mature tissue. Mini-pig neural stem cells contained GM2 and GM3 as major gangliosides. In order to study their distribution in mini-pig neural stem cells and its possible changes during the differentiation of neuronal cells, cells were stained with specific monoclonal antibodies against 11 ganglio-series gangliosides including those mentioned above. GM2 and GM3 were expressed in a spatio-temporally different manner during differentiation of mini-pig neural stem cells, but other gangliosides were not immunohistochemically detected. These results suggest that ganglioside GM2 and GM3 play an important role in the regulation of neuronal differentiation of mini-pig neural stem cells through the activation of retionic acid (RA) and basic fibroblast growth factor (bFGF).
Young-Kug Choo, Jae-Sung Ryu, Malg-Um Lim, Mi-Ran Hwang, Hyun-Ki Min, Gislain Moussavou, Ji-Su Kim, Sun-Uk Kim, Kisung Ko, Young-Ho Cho, Sang-Yoon Nam, Kyu-Tae Chang 충북대학교 동물의학연구소 2012 Journal of Biomedical and Translational Research Vol.13 No.4
Flavonoids have a range of biological activities, including anti-allergic, anti-inflammatory, anti-microbial, and anti-cancer activities, as demonstrated by in vitro studies. In this study, we investigated whether luteolin can be applied to suppression of lipopolysaccharide (LPS)-stimulated inflammatory responses in murine macrophages. Luteolin was found to reduce nitric oxide (NO) production in LPS-stimulated Raw 264.7 cells. In addition, expression of inducible nitric oxide synthetase (iNOS), cyclooxygenase-2 (COX-2), and pro-inflammatory cytokine tumor necrotic factor-α (TNF-α) at the mRNA and protein levels were decreased. These inhibitory effects were found to be caused by the blockade of nuclear factor kappa-light- chain-enhancer of activated B cells (NF-κB) activation and phosphorylation of mitogen-activated protein (MAP) kinase family, extracellular signal-regulated kinases 1/2 (ERK1/2), c-Jun N-terminal kinase (JNK), and p38 MAP kinase. In addition, pre-treatment with luteolin resulted in reduced ganglioside expression levels and inhibited expression of GT1b in Raw 264.7 cells. On the basis of these observations, we suggest that luteolin has potential as an anti-inflammatory drug candidate, and ganglioside GT1b may play a role in the inflammatory process.
유재성,Malg-Um Lim,Mi-Ran Hwang,Hyun-Ki Min,Gislain Moussavou,김지수,김선욱,고기성,조영호,남상윤,장규태,추영국 충북대학교 동물의학연구소 2012 Journal of Biomedical and Translational Research Vol.13 No.4
Flavonoids have a range of biological activities, including anti-allergic, anti-inflammatory, anti-microbial, and anti-cancer activities, as shown by in vitro studies. In this study, we investigated whether luteolin can be applied to the suppression of lipopolysaccharide (LPS)-stimulated inflammatory responses in murine macrophages. Luteolin was found to reduce nitric oxide (NO) production from LPS-stimulated Raw 264.7 cells. moreover, expression of inducible nitric oxide synthetase (iNOS), cyclooxygenase-2 (COX-2) and pro-inflammatory cytokine tumor necrotic factor-α (TNF-α) at the mRNA and protein levels were decreased. These inhibitory effects were found to be caused by the blockage of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) activation and the phosphorylation of mitogen-activated protein (MAP) kinase famaily, extracellular signal-regulated kinases 1/2 (ERK1/2), c-Jun N-terminal kinase (JNK) and p38 MAP kinase. Furthermore, pre-treatment with luteolin reduced ganglioside expression levels and inhibited GT1b expression in Raw 264.7 cells. On the basis of these observations, we suggest that luteolin has potential as an anti-inflammatory drug candidate, and ganglioside GT1b may play a role in the inflammatory.
Human leukocytes regulate ganglioside expression in cultured micro-pig aortic endothelial cells
Jin-Hyoung Cho,Ji-Su Kim,Malg-Um Lim,Hyun-Ki Min,Dong-Hoon Kwak,Jae-Sung Ryu,Ju-Taek Lee,Sun-Uk Kim,Chang-Hwan Kim,Chang-Hyun Kim,Deog-Bon Koo,Kyu-Tae Chang,Young-Kug Choo 한국실험동물학회 2012 Laboratory Animal Research Vol.28 No.4
Gangliosides are ubiquitous components of the membranes of mammalian cells that are thought to play important roles in various cell functions such as cell-cell interaction, cell adhesion, cell differentiation, growth control, and signaling. However, the role that gangliosides play in the immune rejection response after xenotransplantation is not yet clearly understood. In this study, the regulatory effects of human leukocytes on ganglioside expression in primary cultured micro-pig aortic endothelial cells (PAECs) were investigated. To determine the impact of human leukocytes on the expression of gangliosides in PAECs, we performed high-performance thin layer chromatography (HPTLC) in PAECs incubated with FBS, FBS containing human leukocytes, human serum containing human leukocytes, and FBS containing TNF-α. Both HPTLC and immunohistochemistry analyses revealed that PAECs incubated with FBS predominantly express the gangliosides GM3, GM1, and GD3. However, the expression of GM1 significantly decreased in PAECs incubated for 5 h with TNF-α (10 ng/mL), 10% human serum containing human leukocytes, and 10% FBS containing human leukocytes. Taken together, these results suggest that human leukocytes induced changes in the expression profile of ganglioside GM1 similar to those seen upon treatment of PAECs with TNF-α. This finding may be relevant for designing future therapeutic strategies intended to prolong xenograft survival.
( Ghislain Moussavou ),( Dong Hoon Kwak ),( Malg Um Lim ),( Ji Su Kim ),( Sun Uk Kim ),( Kyu Tae Chang ),( Young Kug Choo ) 생화학분자생물학회(구 한국생화학분자생물학회) 2013 BMB Reports Vol.46 No.11
Gangliosides are complex glycosphingolipids that are the major component of cytoplasmic cell membranes, and play a role in the control of biological processes. Human mesenchymal stem cells (hMSCs) have received considerable attention as alternative sources of adult stem cells because of their potential to differentiate into multiple cell lineages. In this study, we focus on various functional roles of gangliosides in the differentiation of hMSCs into osteoblasts or neuronal cells. A relationship between gangliosides and epidermal growth factor receptor (EGFR) activation during osteoblastic differentiation of hMSCs was observed, and the gangliosides may play a major role in the regulation of the differentiation. The roles of gangliosides in osteoblast differentiation are dependent on the origin of hMSCs. The reduction of ganglioside biosynthesis inhibited the neuronal differentiation of hMSCs during an early stage of the differentiation process, and the ganglioside expression can be used as a marker for the identification of neuronal differentiation from hMSCs. [BMB Reports 2013; 46(11): 527-532]