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가장자리를 경사지게 만든 슬랩바닥에 대한 계절별 열손실량의 계산
Kusuda T.,Mizumo M.,Bean J. W. 대한설비공학회 1982 설비저널 Vol.11 No.4
DOE-2, BLAST AND NBSLD와 같은 광범위한 에너지 해석 프로그램에 의거하여 효과적으로 가장자리를 경사지게 만든 슬랩에서의 열전달 계산을 할 수 있도록 그 바닥에서 일어나는 열전달 계산을 재현하였다. 슬랩 바닥면으로부터의 깊이에 따른 매달의 평균 온도를 만들어내기 위해 Lachenbruch 방법에 근거를 둔 수치해석 과정을 깊이 연구하였다. Lachenbruch방법으로 얻어진 자료들을 사용하여 슬랩 바닥면으로부터의 깊이에 따른 매달의 평균 온도를 결정하는 간단한 과정을 만들어냈다. 또한, 이 슬랩에서의 열전달을 매 시간별 레스폰스 인자로 해석하는 데에도 이 매달 평균 온도의 자료들을 사용하였다.
Neonatal Research Network of Japan and Neonatal Database
Satoshi Kusuda 대한신생아학회 2023 Neonatal medicine Vol.30 No.1
In 2004, the Neonatal Research Network of Japan initiated a database registration system for high-risk infants in Japan. Currently, 65% of all very low the birth weight infants or infants born at <32 weeks of gestation are registered in the database. Approximately 4,000 new cases are registered annually. A unique feature of the database is that it includes outcome data at 1.5, 3, and 6 years after discharge from neonatal intensive care units. All registered data are fed back annually to the participating hospitals and used to benchmark neonatal care. Results from this database have also been published in many international journals. With the development of this database, neonatal medicine in Japan has progressed.
Effect of trehalose and trehalase production for mycelium growth of Tricholoma matsutake
Mizuho Kusuda,Ryosuke Yodono,Mitsuhiro Ueda,Norifumi Shirasaka,Kazutaka Miyatake,Takao Terashita 한국버섯학회 2010 한국버섯학회지 Vol.8 No.4
Effect of trehalose for the mycelial growth of Tricholoma matsutake were examined. When T. matsutake Z-1 strain was cultured in the partially modified matsutake liquid (PMML) medium and the Hamada matsutake liquid (HML) medium supplemented with trehalose at 24 ℃ for 80days, the vegetative mycelial dry weights showed higher value compared with those of PMML medium and HML medium supplemented with glucose (control). The range of the effect of 1.0-8.0% carbohydrate substrate on vegetative mycelial growth was investigated. The optimal concentration for mycelial growth was 2.0% for the glucose medium but 8.0% for the trehalose medium. To evaluate the potential of the production of trehalase from T. matsutake, the extracellular trehalase activity during the vegetative mycelial growth was measured. The activity of the extracellular trehalase increased during vegetative mycelial growth of T. matsutake and was maximal 70 days after inoculation. This extracellular enzyme was investigated for the purification and the characterization. The partially purified trehalase was obtained from about 1.53l static culture filtrate, with 19.1% recovery, and about 2,940 fold purification. The molecular mass was about 62.6kDa (SDS-PAGE) and 70kDa (Gel-filtration). The enzyme was most active around 40℃ and pH 5.0 and stable over a pH of 4.5~ 6.5 for 30min at 37℃. The enzyme readily hydrolyzed trehalose having α -1,1 glucosidic bond. However, it did not hydrolyze disaccharides such as maltose, iso-maltose, cellobiose, saccharose and lactose.