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Potential Antitumor Activity of SIM-89 in Non-Small Cell Lung Cancer Cells
Jun Pei,Baohui Han,Tianqing Chu,Minhua Shao,Jiajun Teng,Huifang Sha,Aiqing Gu,Rong Li,Jialin Qian,Weifeng Mao,Ying Li 연세대학교의과대학 2017 Yonsei medical journal Vol.58 No.3
Purpose: c-Met and its ligand, hepatocyte growth factor (HGF), play a critical role in oncogenesis and metastatic progression. The aim of this study was to identify inhibited enzymogram and to test the antitumor activity of SIM-89 (a c-Met receptor tyrosine kinaseinhibitor) in non-small cell lung cancer. Materials and Methods: Z’-LYTE kinase assay was employed to screen the kinase enzymogram, and mechanism of action (MOA) analysis was used to identify the inhibited kinases. Cell proliferation was then analyzed by CCK8 assay, and cell migration was determinedby transwell assay. The gene expression and the phosphorylation of c-Met were examined by realtime-PCR and western blotting, respectively. Finally, the secretion of HGF was detected by ELISA assay. Results: c-Met, activated protein kinase (AMPK), and tyrosine kinase A (TRKA) were inhibited by SIM-89 with the IC50 values of 297 nmol/L, 1.31 μmol/L, and 150.2 nmol/L, respectively. SIM-89 exerted adenosine triphosphate (ATP) competitive inhibition on c-Met. Moreover, the expressions of STAT1, JAK1, and c-Met in H460 cells were decreased by SIM-89 treatment, and c-Met phosphorylationwas suppressed in A549, H441, H1299, and B16F10 cells by the treatment. In addition, SIM-89 treatment significantly decreased the level of HGF, which accounted for the activation of c-Met receptor tyrosine kinase. Finally, we showed cell proliferationinhibition and cell migration suppression in H460 and H1299 cells after SIM-89 treatment. Conclusion: In conclusion, SIM-89 inhibits tumor cell proliferation, migration and HGF autocrine, suggesting it’s potential antitumoractivity.
Dynamic angling side-view mirror for supporting recognition of a vehicle in the blind spot
Junpei Kuwana,Makoto Itoh 제어로봇시스템학회 2008 제어로봇시스템학회 국제학술대회 논문집 Vol.2008 No.10
This paper proposes a novel driver support system named DAMS (Dynamic Angling Mirror System) for enhancing driver recognition of a vehicle in the blind spot when his or her vehicle is going to change lanes. The DAMS moves the corresponding side-view mirror dynamically when another vehicle entered the blind spot. A cognitive experiment with a driving simulator was conducted to investigate effectiveness of the DAMS. In addition, we have modified the original DAMS. The new one is named EDAMS (Enhanced Dynamic Angling Mirror System), which turns on a lamp when the system changes the yaw angle of the side-view mirror for supporting driver"s situation awareness. The result of an experiment suggests that EDAMS is more effective and acceptable than the conventional DAMS.
Junpei Zhong,Yu-fai Fung,Mingjun Dai 제어·로봇·시스템학회 2010 International Journal of Control, Automation, and Vol.8 No.3
Particle Filter (PF) is a sophisticated model estimation technique based on simulation. Due to the natural limitations of PF, two problems, namely particle impoverishment and sample size dependency, frequently occur during the particles updating stage and these problems will limit the accuracy of the estimation results. In order to alleviate these problems, Ant Colony Optimization is incorporated into the generic PF before the updating stage. After executing the Ant Colony optimization, impoverished particle samples will be re-positioned and closer to their locally highest likelihood distribution function. Our experimental results show that the proposed algorithm can realize better tracking performance when comparing to the generic PF, the Extended Kalman Filter and other enhanced versions of PF.
Phylogenetic Diversity of Bacteria in an Earth-Cave in Guizhou Province, Southwest of China
JunPei Zhou,YingQi Gu,ChangSong Zou,MingHe Mo 한국미생물학회 2007 The journal of microbiology Vol.45 No.2
The objective of this study was to analyze the phylogenetic composition of bacterial community in the soil of an earth-cave (Niu Cave) using a culture-independent molecular approach. 16S rRNA genes were amplified directly from soil DNA with universally conserved and Bacteria-specific rRNA gene primers and cloned. The clone library was screened by restriction fragment length polymorphism (RFLP), and representative rRNA gene sequences were determined. A total of 115 bacterial sequence types were found in 190 analyzed clones. Phylogenetic sequence analyses revealed novel 16S rRNA gene sequence types and a high diversity of putative bacterial community. Members of these bacteria included Proteobacteria (42.6%), Acidobacteria (18.6%), Planctomycetes (9.0%), Chloroflexi (Green nonsulfur bacteria, 7.5%), Bacteroidetes (2.1%), Gemmatimonadetes (2.7%), Nitrospirae (8.0%), Actinobacteria (High G+C Gram-positive bacteria, 6.4%) and candidate divisions (including the OP3, GN08, and SBR1093, 3.2%). Thirty-five clones were affiliated with bacteria that were related to nitrogen, sulfur, iron or manganese cycles. The comparison of the present data with the data obtained previously from caves based on 16S rRNA gene analysis revealed similarities in the bacterial community components, especially in the high abundance of Proteobacteria and Acidobacteria. Furthermore, this study provided the novel evidence for presence of Gemmatimonadetes, Nitrosomonadales, Oceanospirillales, and Rubrobacterales in a karstic hypogean environment.
Junpei Isozaki,Balaji Veerasamy,Wataru Kitagawa,Takaharu Takeshita 전력전자학회 2015 ICPE(ISPE)논문집 Vol.2015 No.6
This paper presents duality of PWM (Pulse Width Modulation) strategies for reducing the DC ripples between current and voltage source AC/DC converters. The authors propose the PWM strategy of a voltage source converter for reducing the DC current ripples using the PWM strategy of a current source converter. The validity of the duality of the PWM strategies between current and voltage source converters is verified by experiments.
JunPei Zhou,Yanyan Dong,Yajie Gao,Xianghua Tang,Junjun Li,YUN-JUAN YANG,Bo Xu,Zhenrong Xie,Zunxi Huang 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.4
The 774-bp pectate lyase gene plyAI4 from Bacillus sp. I4 was cloned and expressed in E. coli. The gene encodes a 257-residue polypeptide (PlyAI4, 28.3 kDa)with the highest identities of 97.3% with a putative pectate lyase from Bacillus subtilis BSn5 (ADV94306) and 60.3%with an identified pectate lyase of the polysaccharide lyase family (PL) 3 from Paenibacillus amylolyticus 27C64(ADB78774). The purified recombinant PlyAI4 (rPlyAI4)exhibited apparently optimal activity at pH 10.5 ~ 11.0 and 50oC. Compared with the majority of reported alkaline pectate lyases, rPlyAI4 exhibited more residual enzyme activity at 20oC (~45%) or at 70oC (~50%) and better thermostability at 70oC (~60 min half-life at 70oC). In the presence of 20% (v/v) ethanol, pectate lyase activity was enhanced by 0.2 fold. After incubation in 40% (v/v)ethanol at 37oC and pH 8.5 for 1 h, the purified rPelAI4retained more than 75% of the initial activity. Sequence analysis proposed a new signature block, A-D-G-[V/I]-H,for PL 3 pectate lyases. These properties may prove to be important with regards to PlyAI4 for basic research and industrial application.
Stereolithographic biomodeling of equine ovary based on 3D serial digitizing device
Junpei KIMURA,Nobunori Kakusho,Kenji Yamazawa,Yuuko Hirano,Yasuo Nambo,Hideo Yokota,Ryutaro Himeno 대한수의학회 2009 JOURNAL OF VETERINARY SCIENCE Vol.10 No.2
The 3D internal structure microscopy (3D-ISM) was applied to the equine ovary, which possesses peculiar structural characteristics. Stereolithography was applied to make a life-sized model by means of data obtained from 3D-ISM. Images from serially sliced surfaces contributed to a successful 3D reconstruction of the equine ovary. Photopolymerized resin models of equine ovaries produced by stereolithography can clearly show the internal structure and spatial localizations in the ovary. The understanding of the spatial relationship between the ovulation fossa and follicles and/or corpora lutea in the equine ovary was a great benefit. The peculiar structure of the equine ovary could be thoroughly observed and understood through this model.