Identification of Genes Modulated by High Extracellular Calcium in Coculture of Mouse Osteoblasts and Bone Marrow Cells by Oligo Chip Assay

Kim, Hyung-Keun,Song, Mina,Jun, ji-Hae,Woo, Kyung-Mi,Kim, Gwan-Shik,Baek, Jeong-Hwa The Korean Academy of Oral Biology 2006 International Journal of Oral Biology Vol.31 No.2

Calcium concentration in the bone resorption lacunae is high and is in the mM concentration range. Both osteoblast and osteoclast have calcium sensing receptor in the cell surface, suggesting the regulatory role of high extracellular calcium in bone merabolism. In vitro, high extracellular calcium stimulated osteoclastogenesis in coculture of mouse osteoblasts and bone marrow cells. Therefore we examined the genes that were commonly regulated by both high extracellular calcium and 1,25(OH)_(2)vitaminD_(3)(VD3) by using mouse oligo 11 K gene chip. In the presence of 10 mM[Ca^(2+)]e or 10 nM VD3, mouse calvarial osteoblasts and bone marrow cells were co-cultured for 4 days when tartrate resistant acid phosphatase-positive multinucleated cells start to appear. Of 11,000 genes examined, the genes commonly regulated both by high extracellular calcium and by VD3 were as follows; 1) the expressions of genes which were osteoclast differentiation markers or were associated with osteoclastogenesis were up-regulated both by high extracellular calcium and by VD3; trap, mmp9, car2, ctsk, ckb, atp6b2, tm7sf4, rab7, 2) several chemokine and chemokine receptor genes such as sdf1, scya2, scyb5, scya6, scya8, scya9, and ccr1 were up-regulated both by high ectracellular calcium and by VD3, 3) the genes such as mmp1b, mmp3 and c3 which possibly stimulate bone resorption by osteoclast, were commonly up-regulated, 4) the gene such as c1q and msr2 which were related with macrophage function, were commonly down-regulated, 5) the genes which possibly stimulate osteoblast differentiation and/or mineralization of extracellular matrix, were commonly down-regulated;slc8a1, admr, plod2, lox, fosb, 6) the genes which possibly suppress osteoblast differentiation and/or mineralization of extracellular matrix, were commonly up-regulated;s100a4, npr3, mme, 7) the genes such as calponin 1 and tgfbi which possibly suppress osteoblast differentiation and/or mineralization of extracellular matrix, were up-regulated by high extracelluar calcium but were down-regulated by VD3. These results suggest that in coculture condition, both high extracellular calcium and VD3 commonly induce osteoclastogenesis but suppress osteoblast differentiation/mineralization by regulating the expression of related genes.

후방골절단술과 Skeletal anchorage system를 이용한 사악구치부압하 : 증례보고 CASES REPORT

임광수,풍무걸,김기형,이희철,김우형,김동우 대한악안면성형재건외과학회 2002 Maxillofacial Plastic Reconstructive Surgery Vol.24 No.1

The severe extrusion of the maxillary [post.teeth after the early loss of the mandibular post.teeth makes the fabrication of the mandibular prothesis difficult, and without proper planning.the treatment results are often unsatisfactoy. Several treatment modalitys have been introducted to correct the interarch space deficency.:Reduction the occlusal surfaces,Crown lengthening,Endodontics & restorative approach,Extraction, Orthodontic intrusion and segmental osteotomy. Because of many advantages,conservative method is widely used if it has no particular limits of treatment adjustment, but the cases of surgical correction indication are found in clinical experience. In our clinic,we corrected severe extruded maxillary post.teeth by performance of a unilateral posterior osteotomy with superior repositioning of the segment and orthodontic intrusion using Skeletal anchorage system,so here we reported the cases with a study of documents.

대구시 아파트지역의 분리수거 및 재활용에 관한 연구

우형택,곽형숙 한국환경과학회 1995 한국환경과학회지 Vol.4 No.3

Recycling is receiving increasing social attention today as our nation begins to grapple with the significant problems caused by huge amount of municipal solid waste. The topic of recycling is not simple but extremely complicated. This study attempts to provide basic data and policy options for expanding and improving separated collection and recycling in public residential areas, through three case study of apartment housing areas in Taegu Metropolitan City. The main results of this study are summarized as follows. For the significant period of time, all three case areas had in common the extreme difficulty in establishing and operating the system of connecting public participation, collection and storage, transportation, and actual recycling of materials because of a variety of problems involved in this process. Both amounts of and prices for collected materials fluctuated considerably over time mainly due to monthly changes in recyclable home materials and the dynamic nature of recycling markets. Public questionnaire survey revealed the very high level of participation in separated collection, not only because almost all respondents well understood the necessity and importance of recycling, but because they also knew how to do separated collection. But overall activities were rated low and most respondents suggested the enlargement of public participation, the improvement of collection and storage facilities, and collection transportation networks. In particular, most respondents had little experience of using recycled products and used mainly reproduced soap and bathroom tissue. Furthermore, they were considerably unsatisfied with low variety and quality of recycled products, their high prices and low availability in the market. Finally potential policy options and activities for improving separated collection and recycling are suggested.

환경의식의 종합적 측정을 위한 설문지 개발

우형택,문연화 대구효성가톨릭대학교 1998 연구논문집 Vol.58 No.2

With respect to the increasing importance of understanding our environmental concerns, this study was conducted to develop the questionnaire for the comprehensive and objective measurement of environmental concerns and test its reliability. The major research methods taken include the design of environmental questionnaire based on literature review and related theories, and the process of increasing the reliability of developed questionnaire through the repetition of reliability analysis. The total of 3 surveys were conducted and the total of 510 persons including diverse sample groups were questionnaired. The SAS PC+ package program was used in the reliability analysis of surveyed data. The first designed questionnaire consisted of 50 items including 25 items related to environmental pollution and 25 items related to nature conservation, reflecting various factors of environmental concerns. The result of the first reliability analysis showed that Cronbach-αfor the total was 0.75. To increase the reliability, items having low correlation with the total were eliminated, corrected, or revised. The reliability of redesigned questionnaire consisting of 46 items was significantly improved to the level of Cronbach-α0.88, the high reliability of measurement. In the second reliability analysis, 11 items were further revised and corrected to develop the questionnaire having the very high reliability. The reliability of the finalized questionnaire was further increased to the level of Cronbach-α0.92.

成功的인 軍指揮官에 關한 考察

禹亨武 충남대학교 행정대학원 1985 行政硏究 Vol.5 No.-

신증후출혈열 환자의 혈청학적 및 분자생물학적 진단 검사법 비교

우영대,문희주,배형준 대한의생명과학회 2000 Journal of biomedical laboratory sciences Vol.6 No.2

우리나라에서 발생하고 있는 급성 출혈성 질환인 신증후출혈열의 원인 바이러스는 Family Bunyaviridae의 Genus Hantavirus에 속하는 한탄과 서울바이러스에 의하여 발생되고 있다. 본연구에서는 신증후출혈열로 의뢰된 환자에서 한탄바이러스에 대한 항체가를 간접면역형광항체법(indirect immunofluorescent antibody technique, IFAT), 면역효소측정법(enzyme-linked immunosorbent assay, ELISA) (IgG, IgM), 고비중입자응집반응(high density composite particle agglutination, HDPA) 및 플라크감소중화시험(plaque reduction neutrafization test, PRNT)등으로 비교 측정하였고, 신증후출혈열환자로 확진된 15명의 한타바이러스 혈청형을 PRNT와 혈청형 특이 역전사 효소 중합효소연쇄반응(nested reverse transcriptase polymerase chain reaction, nested RT-PCR)으로 확인하였다. 신증후출혈열로 의뢰된 환자에서의 한탄바이러스에 대한 IFAT, ELISA(IgG,IgM),HDPA 그리고 PRNT 비교에서 형광항체, ELISA IgG, 응집항체 및 중화항체는 8명 모두 높게 나타났으며, ELISA IgM은 5명에서는 현저히 높은 항체를 보유하고 있었다. 신증후출혈열 환자 15명에서는 높은 형광항체와 중화항체 역가를 나타내었고, 15명 중 12명은 한탄바이러스, 2명은 서울바이러스에 대한 높은 중화항체를 갖고 있었으며, 1명은 두 바이러스에 대하여 동일한 항체 역가를 나타내었으며, 혈청형 특이 primer를 사용한 nested RT-PCR에서는 15명 중 3명과 1명만이 한탄바이러스와 서울바이러스 primer에 대해 RNA가 검출되었다. The etiologic agents of haemorrhagic fever with renal syndrome(HFRS) in Korea are Hantaan and Seoul virus in the genus Hantavirus, family Bunyaviridae. Antibody titers of sera from HFRS patients against Hantaan virus were measured by immunofluorescent antibody technique(IFAT), enzyme-linked immunosorbent assay (ELISA), high density composite particle agglutination (HDPA) and plaque reduction neutralization test (PRNT). PRNT and nested reverse transcriptase polymerase chain reaction (nested RT-PCR) was used for serotypic differentiation of Hantaviruses against Hantaan and Seoul virus. Eight doubtful HFRS patients showed higher fluorescent, IgG ELISA, agglutination and neutralizing antibody titer by IFAT, ELISA IgG, HDPA and PRNT, respectively. Five out of them showed high IgM antibody titer by IgM capture ELISA against Hantaan virus, remarkably. Fifteen HFRS patients showed higher fluorescent antibody titer by IFAT. In PRNT, 12 out of them showed high neutralizing antibody titer aginst HTNV, 2 against SEOV and 1 against both viruses. In nested RT-PCR using serotype specific-primer, 3 out of them showed positive against HTNV and 1 against SEOV.

수술방법에 따른 액취증의 합병증 비교

김형우, 이의태 충북대학교 의과대학 충북대학교 의학연구소 2012 忠北醫大學術誌 Vol.22 No.1

연구목적 : 액취증은 겨드랑이에서 암내라고 불리는 특징적인 냄새를 내는 증상으로 비교적 흔하면서 환자에게 사회적, 정신적으로 많은 괴로움을 주는 질환이다. 이러한 액취증을 치료하기 위해 과거로부 터 수술적 절제술이 많이 시술되어 왔다. 하지만 불행하게도 수술적 절제술은 여러 가지 합병증이 보고 되고 있다. 현재, 액취증을 치료하기 위해 흡입술이 도입되면서 기존 치료방법과 효과와 만족도에 대한 비교의 필요성이 대두되어 왔다. 대상 및 방법 : 2005년 7월부터 2009년 3월까지 충북대학교병원 성형외과에서 액취증 일차 수술을 한 환자는 총 24명(남자 15, 여자 9)으로 이중 수술적 절제술이 8명, 흡입술이 16명이었다. 추적은 의무기록 검토 및 전화통화를 통한 설문으로 이루어 졌으며, 시술을 받은 24명의 환자중 수술적 절제 술 8명 중 7명(87.5%), 흡입술 16명 중 10명(62.5%)과 인터뷰가 이루어 졌다. 나머지 환자들은 수 신거부, 전화번호변경 등으로 추적하지 못했다. 인터뷰가 이루어진 17명의 환자들은 최소 10개월(평균 27.3개월) 간의 추적기간을 거쳤다. 액취제거에 대한 각 시술의 효과는 매우좋음, 좋음, 보통, 나쁨, 시술에 대한 만족도는 매우만족, 만족, 보통, 불만족 이렇게 각각 4단계로 분류하였다. 결과 : 효과에 대해서 수술적 절제술을 시행한 환자군에서는 매우좋음 4명(57.1%), 좋음 3명(42.9%) 으로 모두 좋음 이상의 답변을 하였고 흡입술을 시행한 환자군에서는 매우좋음 1명(10%), 좋음 5명 (50%), 나쁨 4명(40%)으로 답변을 하였다. 만족도에 대해서 수술적 절제술을 시행한 환자군 중 5명 (71.4%)이 만족 이상을 답변하였고, 흡입술을 시행한 환자군 중 4명(40%)이 만족 이상을 답변하였다. 합병증으로 수술적 절제술의 경우 과색소침착을 동반한 경계부위 조직괴사 및 과색소침착, 상처개열, 혈 종 각 1명으로 나타났고(총 3/7, 42.9%), 흡입술에서는 어떠한 합병증도 발견되지 않았다(0%). 결론 : 수술적 절제술 환자의 효과 및 만족도가 더 높지만 합병증이 흔한 것으로 나타났으며 흡입술의 경우 합병증은 없지만 효과 및 만족도가 떨어지는 것으로 나타났다. 이에 따라 두가지 방법 어느 하나 가 다른 하나에 비해 반드시 우월하다고 볼 수는 없는 것으로 보이며 환자와 상황에 따라서 알맞은 방 법을 쓰는 것이 좋을 것으로 생각된다.

한국 중학생의 환경태도 평가를 위한 측정도구 개발

우형택 한국환경과학회 2003 한국환경과학회지 Vol.12 No.7

This study was conducted to design long and short forms of reliable and valid Likert-type scales to measure environmental attitudes of Korean junior high school students. 8 tests were applied to the construction of a 32-item long form and 8-item short form of the scale. Two scales were field tested on 469 respondents consisting of male and female students in urban and rural areas and students participating in environmental programs. The 32-item scale was revealed to be highly reliable, content and construct valid. Reliability and homogeneity of this scale were evidenced by 0.92 coefficient alpha and positive interitem correlation values ranging from 0.10 to 0.52. Factor analysis and known-group comparison showed the sufficient validity of long form scale. Reliability and validity of short form scale were also evidenced by good results of 8 test analyses. The long and short forms were strongly correlated, r-value of 0.90. Consequently, both long form and short form scales were evidenced very reliable and valid in measuring environmental attitudes of junior high school students in Korea. They can be useful for a variety of survey conditions and constraints and testing environmental attitude.

공진 기초를 이용한 아스팔트 콘크리트의 동적 전단계수 추정에 관한 연구

우제윤,김수일,이형인 연세대학교 산업기술연구소 1985 논문집 Vol.17 No.2

In this study, a resonant footing technique to estimate the dynamic shear modulus of asphalt concrete using the torsional vibration test is developed. From this study an empirical formula is proposed to estimate the dynamic shear modulus of asphalt concrete. The numerical results are compared with the values estimated from the measurement of primary wave velocities. The oscillator made for this study has thin circular bars which are considered as springs. Heavy upper part of the oscillator is connected to lower aluminum plate by means of springs. The oscillator is designed so that torsional vibration of aluminum plate is caused by magnetic force and footing-asphalt concrete system also vibrates together with aluminum plate. From the experimental result, the maximum difference of the dynamic shear modulus from the resonant footing test and primary wave velosity detection shows 3% deviation when the Poisson`s ratio is assumed to be 0.3. The apparatus made for this study is thought to estimate the dynamic shear modulus of asphalt concrete properly. It is also found that the dynamic shear modulus is not influenced by confining pressure within the range of confining pressure applied in this study.

일회 호흡법(Single breath method)을 사용한 심박출량 측정

우성,조형상 중앙대부설한국의과학연구소 1984 한국의과학 Vol.16 No.4