냉동 제대혈 세포의 체외 증폭

김삼용,김철희,배광봉,김현수,박상준,김종숙,윤환중,조덕연 충남대학교 암연구소 1998 癌共同硏究所 硏究誌 Vol.2 No.1

Background : Cord blood(CB), which has no HLA restriction, is an alternative to bone marrow for hematopoietic stem cell transplantation. The use of cord blood, however, is limited by the number of progenitor/stem cells necessary to reconstitute the older child or adult. Therefore, ex vivo expansion of CB could have tremendous impact on diverse clinical settings. We studied the ex vivo expansion of isolated population of CD34_(+) cells from cryopreserved CB cells. Methods : CD34 cells were isolated from cryopreserved CB mononuclear cells. Purified cells were cultured with various combinations of hematopoietic growth factors including erythropoietin(EPO), stem cell factor(SCF), granulocyte-colony-stimulating factor(G-CSF), gra-nulocyte, macrophage-colony-stimulating factor(GM-CSF), interleukin-1β(IL-1β), 1L-3, and IL-6. After 7, 10 or 14 days of culture, the fold increases of colony-forming unit- granu-locyte, macrophage(CFU-GM), burst-forming unit-erythroid(BFU-E), colony-forming unit-mix (CFU-Mix), and high proliferative potential colony-forming cell(HPP-CFC) were evaluated. Results : Ten-day culture with the combination of EPO, SCF, G-CSF, IL-1β, and IL-3 resulted in a median of 60-fold increase of CFU-GM, which was greater than those with the combinations of less than 5 growth factors. The addition of IL-6 or GM-CSF to this combination did not enhance CFU-GM expansion. Ten-day culture was significantly superior to 7-day culture for CFU-GM expansion. Prolongation of culture to 14 days, however, revealed decreased expansion of CFU-GM compared to 10 days. BFU-E and CFU-Mix were expanded to 2~5 folds in 7-day culture with the combination of EPO, SCF, and G-CSF. Further expansion was not achieved in 10-day culture and colonies disappeared in 14-day culture. HPP-CFC was expanded to a median of 7.5 folds in 7-day culture with the combination of EPO, SCF, G-CSF, IL-1β, IL-3, and IL-6. Neither 10-day or 14 day-culture enhanced expansion of HPP-CFU. Conclusion : Cryopreserved cord blood cells maintain ex vivo expansion potential. In our system, 10-day culture with the combination consisting of EPO, SCF, G-CSF, IL-1β, and IL-3 seems to be adequate for hematopoietic progenitor/stem cell expansion from cryopreserved cord blood cells.

수술 전 진단된 충수돌기 점액류종 1례

허광식,손현화,김동규,박찬국,김만우,김정용,기근홍 조선대학교 부설 의학연구소 2000 The Medical Journal of Chosun University Vol.25 No.1

Primary unruptured appendiceal mucocele is a rare disease. It is very difficult to diagnose preoperatively because of the rarity of classical symptoms and low incidence. Usually it is discovered incidentally during abdominal ultrasonography(US), computed tomography(CT), or appendectomy for acute appendicitis. If untreated, one type of mucoceles may rupture producing a potentially fatal entity known as pseudomyxoma peritonei, therefore preoperative diagnosis is very important. With the advent of colonoscopy, abdominal US and CT, it has been possible to preoperatively diagnose mucocele of appendix. We experienced a case of appendiceal mucocele in a 60 year-old woman who had symptoms of lower abdominal discomfort and right lower quadrant abdominal pain, and it was diagnosed preoperatively by colonoscopy, abdominal US and CT. So we present this case with review of literature.

下道給代金 直接支給과 관련한 發注者의 契約管理 方案에 관한 硏究 : 判例硏究 및 法理分析을 중심으로 Focusing on Analyzing Case and Legal Theories

이종광,김용수 대한건축학회 2005 大韓建築學會論文集 : 構造系 Vol.21 No.1

The purpose of this study is to conceptualize the legal conditions regarding direct payment to subcontractor con tamed in Article 14 of The Fair Trade Subcontract Transactions Act and Its Impacts While demonstrating the practical and theoretical matters, this study also suggests ways to improve the owner's contract management methods, which help the management reduce unnecessary claims and commercial disputes The literature research, case study, and legal theory analysis are adopted as research methods The primary results of the study are summarized as the following (1) The basic legal relationship among owner, contractor and subcontractor is maintained even when the owner's obligation for prime payment and contractor's obligation for subcontract payment is liquidated according to the legal framework of direct payment to subcontractor (2) When contractor's assets for relevant construction projects are confronted with the seizure (or provisional seizure) claim by the third party creditor, the claim is effective If it occurs before direct payment to subcontractor (3) An owner is not allowed to claim financial compensation from the assets designated for direct payment for contractor's defects liability and responsibility of damage compensation On the other hand, Contractor's debt to subcontractor - e g charge for discounting of a bill, overdue Interest-which has no concern with subcontract money is not comprised m the scope of the money of direct payment (4) In the pre-direct payment to subcontractor phase, it is appropriate for the owner to prevent direct payment to subcontractor because the alteration of the money flow based on the contractual relationship hinder the stabilization of the construction organization In a direct payment to subcontractor phase, however, the owner must manage a series of situations with regard to direct payment to subcontractor An owner must grasp the level of the credit and debt among contractor, subcontractor and contractor's third creditors, while defining the scope of the money of direct payment and the owner must prevent the money utilized for construction from leaking and make It flow into the construction organization

韓國 製造業 部門의 産業災害 決定要因 推定

형광석,조삼용 全南大學校企業經營硏究所 1994 産業經濟硏究 Vol.17 No.1

The Occupational Injuries(hereafter O. I.) is serious problem in Korea. In 1991, about 6 workers died, and 127 thousand working days were lost from O. I. per day on the average. The total economic loss amounted to 8.4% of government expenditure. Meanwhile few economic reserches have been dome on the O. I. in Korea. The existing theories consider the O. I. as an individual problem which results from the individual optimal choice. Therby, they fail to see the social characters of the O. I. and to analyze interactions among the capital, the lavbor, the state. This paper aimed to show the determining factors of O. I. in the manufacturing sector, which is leading sector in economic development of Korea and amounts to 50% of all industrial O. I., and the internal dynamics among the capital, the labor, and the state in Korea. The major findings are as follows : ① the work hours positively have influences on the O. I. The capital-labor ratio and the labor productivity have few influences on the O. I. ② Technology plays a important role in the decreasing of O. I. But it whould be a factor that raises the severity of the O. I. ③ In Korea, increasing of the O. I. correspond to the situation on the suppressing labor control by the state. ④ The O. I. in the heavy-chemical industry is more serious than that in the light industry. The determinants of the O. I. in the manufacturing sector are work hours, the capital labor ratio, labor productivity, technology, industrial property. In conclusion, the O. I. was the products of the internal dynamics of the capital, the labor, and the state.

노인환자에서 전신마취 후 발견된 뇌경색

이광범,김정률,안용미,최준석,박천희 朝鮮大學校 附設 醫學硏究所 2008 The Medical Journal of Chosun University Vol.33 No.3

Perioperative cerebral infarction is rare but may be fatal. This is a case report of acute cerebral infarction following general anesthesia in geriatric patient. A 72 year old female patient was admitted for transabdominal hysterectomy and bilateral saipingooophorectomy and pelvic lymphnode dissection. Preanesthetic assessment revealed a history of hypertension and diabetes mellitus. After surgery, she was alert but she gradually appeared to have right hemiparesis and confusion. Emergency CT brain scan showed early cerebral infarction in the middle cerebral artery territory. She was transferred to the depart ment of neurology and received treatment. She was discharged at 19 days after surgery with improved symptoms.

윤리적 의사결정 관점의 소프트웨어 불법복제에 관한 연구

김광용 한국정보사회진흥원 2004 정보화정책 Vol.11 No.4

본 연구는 소프트웨어 불법복제를 연구함에 있어 윤리적 의사결정 연구 분야에서 많이 활용되고 있는 계획된 행동이론(Theory of Planned Behavior: TPB)을 이용하여, 조직의 구성원들이 어떻게 불법복제를 하고 있는지를 살펴보고자 하였다. 더 나아가 개인의 인식과 조직의 윤리문화가 어떻게 소프트웨어 불법복제에 영향을 미치는지를 파악하여 궁극적으로 불법 소프트웨어 사용의 근절을 위한 적절한 정책이 무엇인지를 파악하고자 하였다. 연구결과 계획된 행동이론은 한국의 조직 구성원들의 소프트웨어 불법복제를 잘 설명하는 모델이며, 조직의 윤리문화는 불법 소프트웨어 사용에 영향을 미치는 중요한 요인이 된다는 것을 실증적으로 입증하여 향후 다양한 소프트웨어 정품사용의 정책 입안에 일조하였다.

인트라넷 이용채택에 관한 연구

이대용,정광진 조선대학교 경영경제연구소 2000 經營經濟硏究 = Management and economics research Vol.23 No.1

독일어의 변모음(Umlaut) 현상에 대한 통시적 고찰

김용경,천성광 부산대학교 사범대학 1995 교사교육연구 Vol.30 No.-

Als "Umlaut" bezeichnet man die Erscheinung der totalen oder partiellen Assimilation eines Stammsilbenvokals an einen Vokal oder Halbvokal der folgenden Silbe. Da solche Vokalvera¨nderungen nur in "Kombination" mit einer bestimmten Lautumgebung eintreten ko¨nnen, werden sie "kombinatorische Lautwandel" genannt. Diese Vorga¨nge sind in der gesamten Sprachgeschichte zu beobachten. Je nach der Art des bewirkenden Vokals spricht man von i-, u-, und a-Umlaut. Der i-Umlat, d. h. Lautwandel des Stammsilbenvokals vor i oder j der unbetonten Folgesilbe, ergreift die folgende : e¨ >i im Germ : a>e im Ahd. (Prima¨rumlaut) ; a>a¨ im Mhd. (Sekunda¨rumlaut) : o>o¨, u>u¨, a￣>a¨￣ (ae ), o￣>o¨￣(oe), u￣>u¨￣ (iu), ou>o¨u¨(o¨u), uo>u¨o¨>u¨□( e) im Mhd (Restumlaut). Der u-Umlaut meint e¨>i-Wandel des Stammsilbenvokals vor u der Folgsilbe im Ahd., der a-Umlaut ("Brechung" nach J. Grimm) u>o(schon im Germ.) oder i>e¨(u¨berwiegend im Ahd.) vor a, e, o der Folgesilbe. Auf diese Umlauterscheinungen gehen die Vokalwechsel zuru¨ck, die im heutigen Deutsch bei Konjugationen der Verben (z. B. geben - gibt, fahren - fa¨hrt), Pluralbildungen der Nomina (z. B. Lamm - La¨mmer), Komparationen der Adjektiven (z. B. lang-la¨nger) und Wortbildungen mit Suffixen (z. B. Berg - Gebirge, bauen - Geba¨ude) oft eintreten.

중ㆍ고등학생 응급처치 지식수준에 관한 실태 조사

홍경용,남철현,최상복,위광복,김기열 韓國保健敎育.健康增進學會 2001 보건교육건강증진학회지 Vol.18 No.3

This study was conducted to examine middle and high school students' knowledge level of first aid, health promotion, and safety. Data were collected from 1.165 students from March 6, 2000 to April 4, 2000. The results of this study are summarized as follows. 1.80.1% of the respondents felt the necessity of first aid. The sources of information about methods of first arid were school (43.9%), mass media (15.6%), and self-study (10.5%). 30.0% of them never leaned the methods of first aid. 2.In the case of obtaining information on first aid through mass media, 58.2% of them got it from TV. 7.3% of them obtained it from magazines, while 5.8% of them got it from newspapers. According to their viewpoints of adequate time to teach the methods of first aid, 38.6% of them thought that the methods had to be taught in the primary school and 25.2% of them thought that they had to be taught in the kindergarten. 24.9% of them replied that the methods had to be taught in the middle school, while 11.3% of them replied that they had to be taught in the high school. 3.The points for knowledge of first aid were 98.29±20.91 on the basis of 150 points (65.5 points on the basis of 100 points). The points showed significant differences in the variables of sex, education level, numbers of siblings, parents' education level, and guardian's occupation.

모바일 비즈니스 수익 모델 분류에 관한 탐색적 연구

이대용,정광진,김희철 조선대학교 경영경제연구소 2002 經營經濟硏究 = Management and economics research Vol.25 No.2