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Purification and Characterization of Heat-Tolerant Protease Produced by Bacillus polyfermenticus SCD
Gooi Hun Choi,Mi Na Jo,Jin Man Kim,Cheon Jei Kim,Kee Tae Kim,Hyun Dong Paik 한국미생물생명공학회(구 한국산업미생물학회) 2013 Journal of microbiology and biotechnology Vol.23 No.11
A protease produced by Bacillus polyfermenticus SCD was purified and characterized as a new detergent material. The protease was purified from supernatant produced by B. polyfermenticus SCD, by ammonium sulfate precipitation, ion-exchange chromatography on a DEAE-Sephadex A-50, and finally gel filtration chromatography on Sephadex G-50. The molecular mass of this enzyme was 44 kDa based on SDS-PAGE. The optimum temperature and pH were 50oC and pH 8.0. The ranges of its stability to the pH and temperature were 7.0 to 9.0 and under 40oC, respectively. The enzyme was highly stable in the presence of the surfactants like Triton X-100 (0.1%), showing a 2-fold increase in its proteolytic activity. However, the enzyme was slightly inhibited by the chelating agent EDTA (1 mM). The enzyme has a maximum activity at 50°C and the activity can be increased by surfactants such as Triton X-100 and Tween 80.
Mobile taxi booking application service’s continuance usage intention by users
Weng, Gooi Sai,Zailani, Suhaiza,Iranmanesh, Mohammad,Hyun, Sunghyup Sean Pergamon 2017 Transportation Research Part D. Transport & Enviro Vol.57 No.-
<P><B>Abstract</B></P> <P>The long-term development of a mobile booking taxi application service depends on the continued use of its passengers. The aim of this study is to investigate the determinants of the mobile taxi booking application service’s continuance intention, using the technology continuance theory by including the perceived risk and subjective norms. The data were collected by surveying 387 users of the mobile taxi application service. The data were analysed by applying the partial least squares technique. The analysis showed that the technology continuance theory has extensive power to explain the continuance intention to use the mobile booking taxi application. Subjective norms also have a significant effect on the attitude of mobile booking taxi application users which represents an important contribution to technology continuance theory extension. The theoretical and practical significances of the study have been discussed.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The early acceptance of an MTB Apps does not guarantee continuation. </LI> <LI> Studies on continuance intention of users toward MTB App use are still lacking. </LI> <LI> Results will help App developers to grasp the factors that result in continued MTB App use. </LI> </UL> </P>
Multi-Agent System for Optimization of Microgrids
Foo Y.S. Eddy,Gooi H.B 전력전자학회 2011 ICPE(ISPE)논문집 Vol.2011 No.5
Microgrids are low voltage networks usually located at the consumer end of the distribution system. It typically consists of consumer loads, energy storage and small generation systems and is capable of islanding to protect itself against grid supply interruption. With the increased awareness of clean energy power systems, renewable technologies such as solar PV, wind turbines and fuel cells are gradually emerging within the power system network, and control and management for these equipment are necessary to ensure the stable operation of microgrids. However, existing centralized control systems are unable to handle the large number of renewable components and thus, a decentralized control scheme called Multi-Agent System (MAS) is introduced to manage these components. The implementation of distributed control will include JADE as the platform for agent communications as well as developing customizable agents for specific microgrid requirements such as ancillary services, power trading and negotiation and network security.
최귀헌,이장현,조미나,윤여창,백현동,Choi, Gooi-Hun,Lee, Jang-Hyun,Jo, Mi-Na,Yoon, Yo-Chang,Paik, Hyun-Dong Korean Society for Food Science of Animal Resource 2008 한국축산식품학회지 Vol.28 No.1
The cell growth and antioxidant activity of Bacillus polyfermenticus SCD were studied in tryptic soy broth (TSB) medium and whey protein concentrate (WPC)-based medium. Overall, higher lactose contents in WPC-35 medium (up to 2.0%), and longer culture times correlated with greater cell viability. In WPC-35 medium with 1.5% and 2.0% lactose, the cell growth of B. polyfermenticus SCD was similar to growth in TSB medium. The 1,1-diphenyl-2-picyrylhydrazyl (DPPH) radical scavenging activity of culture supernatant of B. polyfermenticus SCD in WPC-35 medium was measured to assess antioxidant activity. The antioxidant activity increased up to 32 hr of culture, reaching a maximum of 75.57% DPPH radical scavenging activity. The antioxidant activity seemed to follow the typical kinetics of primary metabolite synthesis. The antioxidant activity of B. polyfermenticus SCD supernatant in WPC-35 medium was more effective and stable than supernatant from TSB medium. These results suggest that WPC-35 medium is effective for the production of antioxidant by B. polyfermenticus SCD.
Jang-Hyun Lee,Mi-Seung Chae,Gooi Hun Choi,Na-Kyoung Lee,Hyun-Dong Paik 한국식품과학회 2009 Food Science and Biotechnology Vol.18 No.4
Production of the antioxidant substances by Bacillus polyfermenticus SCD was investigated using shake-flask fermentation. The one-factor-at-a-time method was first employed to determine the key ingredients for optimal medium composition, then further investigation of the medium composition was performed using response surface methodology (RSM). The antioxidant activity was measured using 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) assays. After screening various elements, fructose, tryptone, and MgSO₄ㆍ7H₂O were chosen as the main factors for study in the statistical experimental design. Central composite design (CCD) was then used to determine the optimal concentrations of these 3 components. Under the proposed optimized medium containing 2.8% fructose, 1.34% tryptone, 0.015% MgSO₄ㆍ7H₂O, 0.5% NaCl, and 0.25% K₂HPO₄, the model predicted an antioxidant activity of 80.5% (R²=0.9421). The actual experimental results were in agreement with the prediction.
Kim, Do-Hee,Lee, Hyun-Jung,Choi, Gooi-Hun,Kim, Ok-Hyeon,Lee, Kwang-Gill,Yeo, Joo-Hong,Lee, Jun-Young,Lee, Sang-Hyung,Youn, Young-Chul,Lee, Jang-Han,Paik, Hyun-Dong,Lee, Won-Bok,Kim, Sung-Su,Jung, Hee- Korean Society for Food Science of Animal Resource 2009 한국축산식품학회지 Vol.29 No.2
Previous studies indicate that BF-7 enhances learning and memory in normal and elderly individuals. Here, we evaluated whether milk containing BF-7 (BF-7 milk) could improve the brain function, with thirty normal university students $(21{\pm}1.2 years)$. Two versions of the Paced Auditory Serial Addition Test were used under double-blinded conditions to measure the efficacy of BF-7 milk on learning and memory, especially working memory and attention, and on mathematical ability. As a result, BF-7 milk improved the accuracy of the task more than 3-fold. Furthermore, BF-7 milk protected cultured neuronal cells from 3-hydroxykynurenine, a normal endogenous brain stress agent. These results indicate that BF-7 milk enhances memory, attention and mathematical ability in normal persons.
BF-7 강화 우유의 뇌기능보호 효과 및 물리화학적 특성
최귀헌,조미나,문선희,임성민,정아람,윤여창,백현동,Choi, Gooi-Hun,Jo, Mi-Na,Moon, Sun-Hee,Lim, Sung-Min,Jung, A-Ram,Yoon, Yoh-Chang,Paik, Hyun-Dong Korean Society for Food Science of Animal Resource 2008 한국축산식품학회지 Vol.28 No.4
The impact of storage on the neuroprotective effects against $A\beta$-induced cell death and physicochemical characteristics of milk fortified with BF-7 were investigated. The BF-7 milk exerted protection of neuronal cells SK-N-SH from amyloid beta ($A\beta$)-induced neuronal stress. Our results showed that incubation of the cell with pretreated BF-7 milk, significantly attenuated apoptotic stress by $A\beta$, considered in cell morphology and nucleus shape. The general compositions were maintained consistently in BF-7 fortified milk (BF-7 milk). The BF-7 did not make any disturbance on pH and titratable acidity. The color change was not detected, either. Also, any microorganism had not been detected with more than 7 days storage at $4^{\circ}C$. In sensory evaluation study. the average scores of each sensory attribute were quite similar with plain milk. In conclusion, our results strongly indicate that BF-7 characteristics are quite adequate to be included in milk and BF-7 milk is still working well on neuro-protection, result in enforcing our brain and delaying neurodegeneration.
글루티노사오리나무의 생장과 재질에 관한 연구 -물리적 성질-
한주환 ( Ju Hwan Han ),정재훈 ( Jae Hun Jeong ),이귀용 ( Gooi Yong Lee ),김병로 ( Byung Ro Kim ) 한국목재공학회 2015 목재공학 Vol.43 No.1
본 연구는 4개 종자산지(Yugoslavia, Italy, United Kingdom, Bulgary)의 글루티노사오리나무(24년생)를 대상으로 물리적 특성을 조사하여 생장(평균연륜폭)과 목재의 재질관계를 규명하였다. 함수율, 밀도, 수축률 및 T/R비는 모두 산지간에 차이가 없었다. 또한 이런 성질들은 생장과도 관계가 없는 것으로 나타났다. 종자산지가 다른 글루티노사오리나무의 중요한 재질지표인 밀도와 수축률의 경우, 생장의 차이에 따라 밀도와 수축률이 차이가 거의 없는 것으로 나타나, 생장이 우수한 것이 우수품종의 기준일 것으로 생각된다. Relationship between annual ring width and physical properties of 24-year old alder woods (Alnus glutinosa L.) that had different seed origins from four provenances such as Bulgary, Italy, United Kindom, and Yugoslavia was investigated. No difference among different origins of seeds was found for the moisture content, density, and shrinkage or tangential radial (T/R) ratio. These properties had also no relationship with the growth rate. As important parameters among wood quality indices, no difference in the density or shrinkage on wood that has different growth rates was detected among alder woods from four different origins of seeds. Therefore, it is considered that higher growth rate might be a key factor for selecting the seed origin of the alder tree.