세포응집물질을 생산하는 Aspergillus sp. LAM 94-142의 특성 및 물질생산 조건

이동희,이노운,이현우 건국대학교 산업기술연구원 1996 건국기술연구논문지 Vol.21 No.-

토양에서 분리한 세포응집물질을 생산하는 균주 LAM 94-142는 형태, 배양 및 생리학적 특성을 조사한 결과 Aspergillus parasiticus 또는 그 근연균으로 동정되었으며 물질을 생산하기 위한 배양최적 조건을 검토한 결과는 다음과 같다. 최적 탄소원과 질소원은 각각 1.5% glucose와 0.35% yeast extract였으며 Ca²+을 첨가하였을 때 물질생산이 현저히 증가되었으며 K+, Cu²+, Fe²+ 등의 금속이온은 물질 생성을 저해하였다. 또 인산의 K염은 물질 생합성을 촉진하였으나 ammonium염은 크게 저해하였다. 그리고 최적 초발 pH와 온도는 pH7과 30℃였으며 4일간 배양하였을 때 물질 생산량이 최대였다. A strain of mold, LAM 94-142, capable of producing cell aggregation factor was isolated from soil and identified as Aspergillus parasiticus or its related strain on the morphological, cultural and physiological characteristics. For production of the cell aggregation factor, glucose and yeast extract was favorable as carbon and nitrogen source, respectively. The factor production was increased by the addition of Ca²+ and potassium phosphates but inhibited by K+, Cu²+, Fe²+ and ammonium phosphates. Optimal initial pH and temperature for the factor production was pH 7 and 30℃, respectively. The production of the factor reached at maximum after 4 days in the medium containing 1.5% glucose, 0.35% yeast extract, 0.07% CaCl₂, and 0.05% KH₂PO₄.

DAF 공정에서 무기 고형입자의 유체역학적 충돌효율과 부상특성

곽동희,김성진,이화경,정흥조,이재욱,정팔진 대한상하수도학회 2002 상하수도학회지 Vol.16 No.6

Separation characteristics of inorganic particles occurred during heavy rainwater were investigated in DAF (dissolved air flotation) process. In order to remove the inorganic particles effectively, the collision and flotation efficiencies were examined from a hydrodynamic point of view. Generally, the collision efficiency increased with floc size under the variation of fluid dynamic conditions including inertial force. However, more precise model should be required to analysis the collision efficiency expressed both the physical properties for inorganic particles and hydrodynamic conditions for a reactor.

메치실린 내성 황색 포도상구균에 유효한 항생물질을 생산하는 방선균의 선별 및 물질생산조건

이동희,홍환석 건국대학교 산업기술연구원 1999 건국기술연구논문지 Vol.24 No.-

토양시료로부터 다제내성 MRSA에 유효한 항생물질을 생산하는 Actinomycetes 균주 LAM 98-90을 분리하여 동정한 결과 Streptomyces 속 균주였으며 spore chain은 spiral, spore surface는 smooth type이었고, 세포벽은 LL-DAP를 함유하였다. Aerial mass color는 회색이었으며 melanoid와 가용성 색소는 생성하지 않았다. 그리고 LAM 98-90에 의한 항생물질의 생산조건을 검토한 결과 탄소원으로는 soluble starch가, 질소원으로는 yeast extract, beef extract, peptone이 가장 우수하였으며 Mg²+ 첨가로 물질생산량이 많이 증가하였다. 생산 최적온도와 초발 pH는 각각 35℃와 7이었으며, soluble starch 2%, yeast extract 0.1%, beef extract 0.1%, peptone 0.1%, MgSO₄7H₂O 0.05%, K₂HPO₄ 0.05%, NaCl 0.05% 조성의 배지로 5일간 배양하였을 때 배양액내의 항생물질 축적량이 최대에 달하였다. LAM 98-90 균주가 생산하는 항생물질은 Gram 양성세균에 대해서는 항균활성이 우수하였으나 Gram 음성세균에는 비교적 약하였으며 진균류에는 전혀 작용하지 못하였다. An antibiotic producing strain LAM 98-90, effective to the multidrug-resistant MRSA, was isolated from soil. The spore chain of LAM 98-90 was spiral, and the spore sulface was smooth. LAM 98-90 has LL-diaminopimelic acid in the cell wall. The aerial mass color of the strain was gray, and the reverse side color was brown. The strain did not produce melanoid and other soluble pigments. And according to the Taxon program, LAM 98-90 was identified to one strain of Streptomyces. For the antibiotic production, soluble starch, soluble starch, yeast extract, beef extract and peptone were favorable as carbon and nitrogen sources. The antibiotic production was stimulated by the addition of Mg²+, but inhibited by Ag+, Co²+ and Fe²+ completely. Optimal temperature and initial pH for production of the antibiotic was 35℃ and pH 7, respectively. Accumulation of the antibiotic in the culture broth reached at maximal level after 5 days cultivation in the medium containing soluble starch 2%, yeast extract 0.1%, beef extract 0.1%, peptone 0.1%, K₂HPO₄ 0.05%, MgSO₄·7H₂O 0.05%, and NaCl 0.05%, and NaCl 0.05%. The antibiotic inhibited the growth of Gram positives well, however moderated for Gram negatives, and did not inhibit yeast and mold.

원수중의 천연유기물이 부상분리공정에 미치는 영향

곽동희,정팔진,이성우 全北大學校 附設 都市및環境硏究所 1997 都市 및 環境硏究 Vol.12 No.1

Many water bodies used for water supply may contain solid particles with a lower density. These solids are hardly removed in the sedimentation unit due to the own tendency of floating. In order to treat this type of water, the flotation process in known to be an effective method. A series of laboratory experiments has been performed to investigate the effect of natural organic matter(NOM) in raw water on flotation process. Foam formation potential(FFP) could be increase by NOM in raw water, alum is stimulate the trend of FFP to increase. Results of DAF treatment studies show that flotation efficiency can be increase by AOM, such as ethyl ester, sodium propionate, sodium dodecylbenzenesulfonate. Meanwhile, flotation efficiency can be decrease by AOM, such as glycerol, starch, pentane, peptone, isoamyl alcohol, humic acid etc. Also, the flotation efficiency by AOM corresponds well the tendency of FFP.

Streptomyces속 균주 YS-87-8에 의한 항산화성 물질의 생산조건

이동희 건국대학교 1989 學術誌 Vol.33 No.2

One Streptomyces sp. capable of producing antioxidant was isolated from soil and named as YS-87-8, and the cultural conditions were studied. For the antioxidant production, sucrose and peptone was favorable as carbon and nitrogen source, respectively. The antioxidant production was slightly increased by the addition of Ca2+, but depressed markedly by Ag+, Co2+, Hg2+, Zn2+, Fe2+ and Fe3+. And the addition of amino acid such as histidine, proline, leucine and glycine, and vitamin such as hesperidin, folic acid and cyanocobalamin increased the production of the antioxidant remarkably. Optimal initial pH and temperature for the antioxidant production was pH 8 and 25℃, respectively. The production of the antioxidant reached at maximum after 7-8 days cultivation in the medium containing 1.5% sucrose, 0.4% peptone, 0.1% K2HPO4, 0.05% CaCl2ㆍ2H2O, 0.05% MgSO4ㆍ7H2O, 0.05% NaCl, 0.005% histidine and 10ppm hesperidin.

빗물의 용도결정을 위한 세균학적 안전성 및 수질 평가

곽동희,독고석,한무영 대한상하수도학회 2003 상하수도학회지 Vol.17 No.5

Recently, there has been an increasing interest to promote the utilization of rainwater. In this paper, concentration of organic compound and heavy metal in rainwater samples was analyzed to examine the hazardous level of rainwater for utilization. And an experiment for separating the opportunistic and pathogenic bacteria was also performed. Unlike the quality of ground water and surface water, only a few couple of bacteria types have found in the rainwater samples. This means that the quality of rainwater is by far safer than other water sources. Many opportunistic colony which inhabits in nature was found in the most of samples while the pathogenic bacteria was not detected. Organic compounds have not found in rainwater. Concentration of heavy metal in it also shows under the regulation of drinking water. Conclusively, on the basis of the analysis results on five samples, rainwater could be used directly for various purpose without additional water treatment process.

Bacillus subtilis가 생산하는 항진균성 항생물질 LAM-44B의 정제 및 특성

이동희,권태종 건국대학교 산업기술연구원 2005 건국기술연구논문지 Vol.30 No.-

A novel antifungal antibiotic for medically isolated azole-resistant Candida albicans was purified from the culture broth of Bacillus subtilis LAM 97-44 by butanol extraction. Diaion HP-20 and Dowex-50 adsorption chromatography, silica gel flash chromatography followed by 1st and 2nd HPLC and designated LAM-44B. LAM-44B was stable for 60min at 100℃, and pH range from 2 to 12. MIC value were observed at 1-6㎍/mL against various Candida albicans strains. The antibiotic showed no acute toxicity to rat, and no cytotoxicity to S180, MKN-45 P388, HeLa and 3T3 cell lines. LAM-44B was colorless powder soluble in water, methanol, butanol and positive to ninhydrin, Molish and Anthrone reaction. The antibiotic had maximum absorption at 281, 292, 305 and 320nm in methanol, and melting point was 232℃. The molecular weight and formula were determined to be 696 and C35H54NO13 by 'H-NMR, 13C-NMR, IR, ESI mass spectrum and elemental analysis.

수용성 항진균성 항생물질을 생산하는 방선균 LAM-593의 분리 및 물질생산조건

이동희,김중배,이노운 建國大學校 附設 産業技術硏究所 1993 논문집 Vol.18 No.-

토양에서 분리 선별한 수용성 항진균성 물질을 생산하는 Streptomyces속 균주 LAM-593에 의한 항진균성 물질의 생산조건을 검토한 결과는 다음과 같다. 물질생산을 위한 탄소원으로는 soluble starch가, 질소원으로는 soybean meal과 peptone이 가장 우수하였으며 glycerol의 첨가로 물질 생성량이 현저히 증가하였으나 인산염에 의해서는 저해되었다. 생산 최적온도와 초발 pH는 각각 30℃와 9.5였으며, soluble starch 1.0%, glycerol 1.0%, soybean meal 0.3%, peptone 0.1%, MgSO₄·7HO 0.05%, NaCI 0.05%, silicone oil 0.01% 조성의 배지(pH 9.5) 2.5L를 5L fermenter에 넣고 전배양한 배양액을 3% 수준으로 접종하여 통기량 0.9vvm, 교반속도 200rpm으로 30℃ 에서 2일간 배양하였을 때 비지내의 항진균성 물질의 축적량이 최대에 달하였다. Cultural conditions for production of water soluble antifungal antibiotic by Streptomyces sp, strain LAM-593, isolated from soil, were studied. For the antibiotic production, soluble starch and soybean meal was favorable as carbon and nitrogen source, respectively. The antibiotic production was stimulated by the addition of Mg? and glycerol. Optimal temperature and initial pH for production of the antibiotic was 30℃ and pH 9.5, respectively. Accumlation of the antibiotic in th culture broth reached at maximum level after 2 days cultivation under aeration rate 0.9 vvm and impeller speed 200rpm in 5L-fermenter with 3L of medium containing soluble starch 1.0%, glycerol 1.0%, soybean meal 0.3%, peptone 0.1%, MgSO₄·7H₄O 0.05% and Nacl 0.05%.

마그네슘 풍부 해양미네랄 용액이 hairless 마우스의 아토피성 피부염에 미치는 영향

김동희,이규재,최주봉,이영미,윤양숙,김정례,장병수,양용석 韓國電子顯微鏡學會 2008 Applied microscopy Vol.38 No.3

Atopic dermatitis (AD) is a chronically relapsing inflammatory skin disease that often has asthma and allergic rhinitis. Magnesium salts, the important component of minerals in Dead Sea water, are known to exhibit beneficial effects in inflammatory disease. Favorable effects of magnesium ions and sea water treated to the skin of patients with contact dermatitis have been reported. But histological and immunological investigations are insufficient. This study was performed to examine the inhibitory effect of magnesium-rich sea mineral water on the development of AD-like skin lesions in hairless mice. AD-like skin lesions are induced by the repeated application of 2,4-dinitrochlorobenzene (DNCB). Local application of magnesium-rich sea mineral water on hairless mice skin applied with DNCB inhibited the development of AD-like skin lesions as exemplified by a significant increase in skin hydration (p<0.01), and a decrease in epidermal water loss (p<0.01). Serum IgE level was also significantly decreased (p<0.01). These results suggest that magnesiumrich sea mineral water inhibits the development of DNCB-induced AD-like skin lesions in hairless mice. These observations indicate that magnesium-rich sea mineral water may be alternative and assistant substances for the management of AD. 아토피성 피부염은 주로 천식과 비염 등을 동반하는, 주위에서 흔히 볼 수 있는 만성 염증성 피부질환으로 유전학적, 환경적, 면역학적 요인이 복잡하게 연관되어 발병한다. 해수에 포함된 마그네슘염은 피부에 작용하여 피부장벽을 보호하는 것으로 알려지고 그에 대한 면역학적인 연구와 조직학적 연구는 아직 부족한 실정이다. 이번 연구에서는 피부염을 인위적으로 일으키는 hapten 형성물질인 DNCB를 hairless mice에 도포하여 아토피 피부염 동물 모델로 만든 후, 마그네슘이 다량 함유된 해양 미네랄수를 처리한 후 피부장벽에 미치는 영향을 관찰하였다. DNCB로 피부염을 유발한 hairless mice에 해양미네랄수를 국소적으로 도포하였을 때 유의한 피부수분함량이 증가와 경피수분손실의 감소를 확인하였다 (p<0.01). 피부측정에서 피부거칠기(skin roughness, p<0.05)와 스케일생성 (skin scaliness, p<0.01)은 실험군에서 유의한 개선효과를 나타내었으며 조직학적 검사에서도 피부손상지수의 유의한 감소 (p<0.01)와 비만세포와 (p<0.01) 호산구의 감소(p<0.05) 소견을 보였고 또한 혈청 IgE의 감소를 관찰할 수 있었다(p<0.01). 이상과 같이 마그네슘이 다량 함유된 해양 미네랄수 도포는 피부장벽의 손상을 줄이고 피부수분손실을 효과적으로 줄임으로 아토피성 피부염 증상 유발을 억제할수 있음을 확인하였다. 현재까지 아토피성 피부염의 관리를 위하여 세라마이드나 식물성 오일의 보습제가 주로 활용되고 있는 상황에서 부가적인 피부장벽의 보호를 위하여 탈염 해양 미네랄수의 활용이 가능할 것으로 판단되며 장기적으로 아토피 피부염치료의 대체, 혹은 보조적 물질로 활용될 수 있을 것으로 기대된다.

Bacillus subtilis LAM 97-44가 생산하는 항진균성 항생물질의 정제 및 특성

이동희,이노운,권태종 한국농화학회 2003 Applied Biological Chemistry (Appl Biol Chem) Vol.46 No.2

병원에서 분리한 azole계 항진균성 항생물질에 대한 내성을 가지고 있는 Candida albicans에 대해 강한 활성을 가지는 항진균성 물질을 Bacillus subtilis LAM 97-44의 배양액으로부터 분리 정제한 후 그 특성을 조사하였다. 원심분리한 배양상등액을 butanol 추출, Diaion HP-20 과 Dowex-50 adsorption chromatography, silica gel flash chromatography와 HPLC로 정제하였고 TLC와 HPLC로 확인하여 그 물질을 LAM-44A라 명명하였다. LAM-44A는 pH와 열에 매우 안정하였으며 Candida sp., Cryptococcus sp. 등에 대해 강한 활성을 나타낸 반면에 독성은 매우 적었다. 분리한 물질은 273 nm에서 최대흡광도를 가진 융점 202˚C의 무색분말이었으며 ninhydrin 반응결과 음성이었고 ^1H-NMR, ^13C-NMR, IR spectrum, 원소분석 등의 결과로 볼 때 분자량 282의 C_14H_34O_5의 화학식을 가진 물질로 동정되었다. A novel antifungal antibiotic for azole-resistant Candida albicans was purified from the culture broth of Bacillus subtilis LAM 97-44 by butanol extraction, Diaion HP-20 and Dowex-50 adsorption chromatography, silica gel flash chromatography followed by HPLC and designated LAM-44A. LAM-44A was stable for 60 min at l00˚C, and pH range from 2 to 10. MIC values were observed at 0.5-3.5 ㎍/㎖ against various Candida albicans strains. The antibiotic showed no cytotoxicity for S180, MKN-45, P388, HeLa and 3T3 at the concentration of 1 ㎎/㎖. LAM-44A was colorless powder soluble in water, methanol, ethanol, butanol and negative to ninhydrin reaction. The antibiotic had maximum absorption at 273 nm in methanol, and melting point was 202˚C. The molecular weight and formula were determined to be 282 and C_14H_34O_5 by ^1H-NMR, ^13C-NMR, IR spectrum and elemental analysis.