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Evolution of ALPPS: The Simpler, Safer and Effective One---TELPP
( Shu You Peng ),( Xu An Wang ),( Cong Yun Huang ),( You Yong Zhang ),( Jiang Tao Li ),( De Fei Hong ),( Xiu Jun Cai ),( Yi Fang Wang ),( Xiao Liang ),( Jian Wei Wang ) 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1
Aims: The characteristic of associating liver partition and portal vein ligation for staged hepatectomy(ALPPS) carries high mortality and morbidity. There is room for improvement. We suggest Terminal Branches Portal Vein Embolization (TBPVE) as a way to compart the liver. As a result, only a single surgical operation is required.This method is termed Terminal branches portal vein Embolization Liver Partition Planned hepatectomy (TELPP). Methods: Patients with unresectable primary or metastatic liver tumor were performed with TELPP. The procedure of TELPP was that in addition to PVE, embolization agent was infused to the terminal branches of portal vein of S5,S8 or S4. CT scan was taken one or two weeks later, and standard liver volume(SLV), FLR and FLR/SLV are calculated. Two weeks later when the FLR and liver function is appropriate, open or laparoscopic hepatectomy is performed. Results: The study included 11patients including hepatocellular carcinoma: n =8, intrahepatic cholangiocarcinoma: n = 1, hilarcholangiocarcinoma: n =1, colorectal liver metastasis: n =1. After a waiting period of 14 days, the volume of theFLR had increased from 382mlto 578ml, representing a median volume increase of 51% (range =32.5%-86.7%). Of the 11patients with hepatectomy, right hemihepatectomy (n=2), extended right hemihepatectomy (n=5), right trisecmentectomy(2), extended left hemihepatectomy (n=1) and left trisecmentectomy(1). No patient died, and no serve perioperative morbidity occurred. Conclusions: ALPPS and all modifications need two-stage operations with a high morbidity and mortality rate. It seems that TELPP is very promising. It has the merit of ALPPS as extraordinarily rapid increasement of FLRvolume, yet the morbidity and mortality is much lower, owing to the fact that unlike ALPPS, there is no two liver raw surfaces left behind in the abdominal cavity to produce bile leak, as only single surgical operation is required
Yin-Hua Zhang,De-Ping Ding,De-Qiang Ma,Juan Li,Lin-Li Chen,Kang-Jian Ao,You-You Tian 연세대학교의과대학 2018 Yonsei medical journal Vol.59 No.9
Purpose: To explore the influence of S100 calcium binding protein A4 (S100A4) knockout (KO) on methionine-choline-deficient(MCD) diet-induced non-alcoholic fatty liver disease (NAFLD) in mice. Materials and Methods: S100A4 KO mice (n=20) and their wild-type (WT) counterparts (n=20) were randomly divided into KO/MCD, Ko/methionine-choline-sufficient (MCS), WT/MCD, and WT/MCS groups. After 8 weeks of feeding, blood lipid and liverfunction-related indexes were measured. HE, Oil Red O, and Masson stainings were used to observe the changes of liver histopathology. Additionally, expressions of S100A4 and proinflammatory and profibrogenic cytokines were detected by qRT-PCR andWestern blot, while hepatocyte apoptosis was revealed by TUNEL staining. Results: Serum levels of aminotransferase, aspartate aminotransferase, triglyceride, and total cholesterol in mice were increasedafter 8-week MCD feeding, and hepatocytes performed varying balloon-like changes with increased inflammatory cell infiltrationand collagen fibers; however, these effects were improved in mice of KO/MCD group. Meanwhile, total NAFLD activity scoresand fibrosis were lower compared to WT+MCD group. Compared to WT/MCS group, S100A4 expression in liver tissue of WT/MCD group was enhanced. The expression of proinflammatory (TNF-α, IL-1β, IL-6) and profibrogenic cytokines (TGF-β1, COL1A1,α-SMA) in MCD-induced NAFLD mice were increased, as well as apoptotic index (AI). For MCD group, the expressions ofproinflammatory and profibrogenic cytokines and AI in KO mice were lower than those of WT mice. Conclusion: S100A4 was detected to be upregulated in NAFLD, while S100A4 KO alleviated liver fibrosis and inflammation, inaddition to inhibiting hepatocyte apoptosis.
You, Dae Jong,Kim, Do Hyung,De Lile, Jeffrey Roshan,Li, Chengbin,Lee, Seung Geol,Kim, Ji Man,Pak, Chanho Elsevier 2018 Applied catalysis. A, General Vol.562 No.-
<P><B>Abstract</B></P> <P>Pd-based core-shell alloy-supported catalysts were prepared sequentially via a microwave-assisted polyol method and galvanic replacement. To investigate the effect of the core composition on the catalytic activity of such catalysts, three different Pd alloy cores (PdNi, PdCu, and PdNiCu) were prepared on carbon supports using a polyol method. Then, Pd and Ir were introduced simultaneously to form shells on the Pd alloy cores by galvanic replacement in aqueous solution, thereby producing catalysts designated as PdNi@PdIr/C, PdCu@PdIr/C, and PdNiCu@PdIr/C. X-ray diffraction revealed that all three catalysts exhibited the face-centered cubic structure of Pd without the presence of individual phases for Ni, Cu, and Ir. The core-shell structure of the Pd-based alloy nanoparticles on the carbon support was verified by the electron energy loss spectroscopy line profile of a 25 nm nanoparticle of PdNiCu@PdIr/C. Among the three Pd-based core-shell catalysts, the highest electrochemical surface area and oxygen reduction reaction (ORR) activity was observed for PdNiCu@PdIr/C. In addition, the membrane electrode assembly employing the PdNiCu@PdIr/C catalyst displayed a significantly improved voltage compared to the other two catalysts under high-temperature polymer electrolyte membrane fuel cell conditions at 150 °C. Single-cell durability tests conducted to measure the voltage change at a constant current density of 0.2 A cm<SUP>−2</SUP> showed a decay ratio of 12.3 μV h<SUP>−1</SUP>. These results suggest that the composition of the core in core-shell nanoparticles has an important influence on both the electronic properties in the Pd alloy core and compressive lattice strain on the PdIr shell. Control of these synergistic effects provides a new approach for developing catalysts with high ORR activity.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Three catalysts with PdNi, PdNiCu and PdCu cores and a PdIr shell were prepared by polyol method and galvanic replacement. </LI> <LI> A membrane electrode assembly with PdNiCu@PdIr/C catalyst showed the significantly improved performance and durability. </LI> <LI> The core component of core-shell catalysts has important role to improve the activity toward the oxygen reduction reaction. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Assembly of Laccase over Platinum Oxide Surface and Application as an Amperometric Biosensor
Quan, De,Kim, You-sung,Yoon, Kyung-Byung,Shin, Woon-sup Korean Chemical Society 2002 Bulletin of the Korean Chemical Society Vol.23 No.3
Laccase could be successfully assembled on an amine-derivatized platinum electrode by glutaraldehyde coupling. The enzyme layer formed on the surface does not communicate electron directly with the electrode, but the enzymatic activity of the surf ace could be followed by electrochemical detection of enzymatically oxidized products. The well-known laccase substrates, ABTS (2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)) and PPD (p-phenylenediamine) were used. ABTS can be detected down to 0.5 ${\mu}M$ with linear response up to 15 ${\mu}M$ and current sensitivity of 75 nA/ ${\mu}M.$ PPD showed better response with detection limit of 0.05 ${\mu}M$, linear response up to 20 ${\mu}M$, and current sensitivity of 340 nA/ ${\mu}M$ with the same electrode. The sensor responses fit well to the Michaelis-Menten equation and apparent $K_M$ values are 0.16 mM for ABTS and 0.055 mM for PPD, which show the enzymatic reaction is the rate-determining step. The laccase electrode we developed is very stable and more than 80% of initial activity was still maintained after 2 months of uses.
Quan, De,Kim, You-Sung,Shin, Woon-Sup Korean Chemical Society 2004 Bulletin of the Korean Chemical Society Vol.25 No.8
Tyrosinase was covalently immobilized on platinum electrode according to the method we developed for laccase (Bull. Korean Chem. Soc. 2002, 23(7), 385) and p-chlorophenol, p-cresol, and phenol could be detected with sensitivities of 334, 139 and 122 nA/ ${\mu}M$ and the detection limits of 1.0, 2.0, and 2.5 ${\mu}M$, respectively. The response time ($t_{90\%}$) is 3 seconds for p-chlorophenol, and 5 seconds for p-cresol and phenol. The optimal pHs of the sensor are in the range of 5.0- 6.0. This sensor can tolerate at least 500 times repeated injections of p-chlorophenol with retaining 80% of initial activity. In case of tyrosinase and laccase co immobilized platinum electrode, the sensitivities are 560 nA/ ${\mu}M$ for p-phenylenediamine (PPD) and 195 nA/ ${\mu}M$ for p-chlorophenol, respectively. The sensitivity of the bi-enzyme sensor for PPD increases 70% compared to that of only laccase immobilized one, but the sensitivity for p-chlorophenol decreases 40% compared to that of only tyrosinase immobilized one. The sensitivity increase for the bi-enzyme sensor for PPD can be ascribed to the additional catalytic function of the co-immobilized tyrosinase. The sensitivity decrease for p-chlorophenol can be explained by the “blocking effect” of the co-immobilized laccase, which hinders the mass transport through the immobilized layer. If PPD was detected with the electrode that had been used for p-chlorophenol, the sensitivity decreased 20% compared to that of the electrode that had been used only for PPD. Similarly, if p-chlorophenol was detected with PPD detected electrode, the sensitivity also decreased 20%. The substrate-induced conformation changes of the enzymes in a confined layer may be responsible for the phenomena.
Baimantuoluosides D-G, Four New Withanolide Glucosides from the Flower of Datura metel L.
Bing-you Yang,Yong-gang Xia,Qiu-hong Wang,De-qiang Dou,Hai-xue Kuang 대한약학회 2010 Archives of Pharmacal Research Vol.33 No.8
In our search for bioactive anti-psoriasis compounds from the flower of Datura metel L, we isolated four new withanolide glucosides, baimantuoluosides D, E, F and G (1-4). The structures of the new compounds are (5α,6α,7β,22R)-5,6,7,27-tetrahydroxy-1-oxowitha-2,24-dien-27-O-β-D-glucopyranoside (1), (5α,6β,7α,22R)-5,6,7,27-tetrahydroxy-1-oxowitha-2,24-dien-27-O-β-D-glucopyranoside (2), (5α,6β,7α,12β,22R)-5,6,7,12,27-pentahydroxy-1-oxowitha-2,24-dien-27-O-β-D-glucopyranoside (3), and (5α,6β,22R)-5,6,27-trihydroxy-1-oxowitha-2,24-dien-27-O-β-D-glucopyranoside (4) on the basis of chemical and physicochemical evidence.