Molecular Cloning and Characterization of a Novel Cold-Adapted Family VIII Esterase from a Biogas Slurry Metagenomic Library

( Xiao Jie Cheng ),( Xu Ming Wang ),( Tian Lei Qiu ),( Mei Yuan ),( Jiang Uang Sun ),( Jun Lian Gao ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.11

A novel esterase gene, est01, was successfully unearthed from a biogas digester microbiota metagenomic library. The 1,194 bp est01 gene encodes a protein of 44,804 Da (designated Est01). The amino acid sequence of Est01 shows only moderate (33%) identity to a lipase/ esterase. Phylogenetic analysis and biochemical characterization confirmed that Est01 is a new member of family VIII esterases. The purified Est01 from recombinant Escherichia coli BL21 (DE3) showed high hydrolytic activity against short-chain fatty acid esters, suggesting that it is a typical carboxylesterase rather than a lipase. Furthermore, the Est01 was even active at 10°C (43% activity remained), with the optimal temperature at 20°C, and had a broad pH range from 5.0 to 10.0, with the optimal pH of 8.0. These properties suggest that Est01 is a cold-adaptive esterase and could have good potential for low-temperature hydrolysis application.

Copy Detection Technique with Enhanced Efficiency by using Substring Comparison Algorithm

Xiao-hui Cheng,Meng Hu,Tong Wang,Yun Deng 보안공학연구지원센터 2016 International Journal of Security and Its Applicat Vol.10 No.12

As the information was being overloaded from time to time, a significant technique should be accomplished by several search engines that exists will use from the databases to eradicate the replicas of data which was available in articles and to present the outcomes of the search to users in terms of percentage of the amount of the data copied from the original file. Plagiarism is the process of bestowing one’s creative ideas as our individual conception. It is does not meant that anybody cannot use other’s considerations or workings, anybody can use their data by giving the actual credit the original users by stating their names in literature or in references and also by giving special note on acknowledgements. Copying the content of the others work is mainly considered to be a big crime in terms of research and in terms of the owning the idea of the others. The main idea of this work is to identify the extent of the data that was being copied or to identify the amount of data that was being copied from other peoples work or their own credited work. Performance of plagiarizing a document is not limited to word files or pdf files, it is possible to plagiarize even images and other files too. Hence, we were focused on finding the plagiarism in word files which includes academics and research articles.

Differential effects on the MICE sustainability and customer loyalty perceived by domestic and international attendees

Cheng, Xiao Xi(정효희),Kim, Chul Won(김철원) 한국전시산업융합연구원 2017 한국과학예술융합학회 Vol.30 No.-

The objective of this study is to explore differential effects of the sustainability perceived by domestic and international participants in the MICE (Meetings, Incentive Travel, Convention, and Exhibition) held in the Republic of Korea and to examine the relationship between customer loyalty and perception of the sustainable MICE. In this study, 30 sustainability variables for the MICE were extracted and tested by the factor analysis, representing four underlying dimensions: economic sustainability, social sustainability, environmental sustainability and event sustainability. The results revealed that significant differences in these four dimensions of the sustainable MICE were perceived by domestic and international participants. The largest difference were perceived by domestic and international participants with respect to the environmental sustainability factor. The mean scores indicated that international participants tended to give higher evaluations than domestic participants. Second, there were statistically significant relationships among economic, environmental, and event sustainability factors and customer loyalty from the perspective of international participants. For domestic participants, only event sustainability had a vital relationship with customer loyalty. The study implied how policy makers and the MICE managers should adopt four dimensions of sustainability to establish a harmonious and sustainable MICE practices.

Functional Magnetic Resonance Imaging in the Diagnosis of Locally Recurrent Prostate Cancer: Are All Pulse Sequences Helpful?

Xiao-Li Liao,Jun-Bao Wei,Yong-Qiang Li,Jian-Hong Zhong,Cheng-Cheng Liao,Chang-Yuan Wei 대한영상의학회 2018 Korean Journal of Radiology Vol.19 No.6

Objective: To perform a meta-analysis to quantitatively assess functional magnetic resonance imaging (MRI) in the diagnosis of locally recurrent prostate cancer. Materials and Methods: A comprehensive search of the PubMed, Embase, Cochrane Central Register of Controlled Trials, and Cochrane Database of Systematic Reviews was conducted from January 1, 1995 to December 31, 2016. Diagnostic accuracy was quantitatively pooled for all studies by using hierarchical logistic regression modeling, including bivariate modeling and hierarchical summary receiver operating characteristic (HSROC) curves (AUCs). The Z test was used to determine whether adding functional MRI to T2-weighted imaging (T2WI) results in significantly increased diagnostic sensitivity and specificity. Results: Meta-analysis of 13 studies involving 826 patients who underwent radical prostatectomy showed a pooled sensitivity and specificity of 91%, and the AUC was 0.96. Meta-analysis of 7 studies involving 329 patients who underwent radiotherapy showed a pooled sensitivity of 80% and specificity of 81%, and the AUC was 0.88. Meta-analysis of 11 studies reporting 1669 sextant biopsies from patients who underwent radiotherapy showed a pooled sensitivity of 54% and specificity of 91%, and the AUC was 0.85. Sensitivity after radiotherapy was significantly higher when diffusion-weighted MRI data were combined with T2WI than when only T2WI results were used. This was true when meta-analysis was performed on a per-patient basis (p = 0.027) or per sextant biopsy (p = 0.046). A similar result was found when 1H-magnetic resonance spectroscopy (1H-MRS) data were combined with T2WI and sextant biopsy was the unit of analysis (p = 0.036). Conclusion: Functional MRI data may not strengthen the ability of T2WI to detect locally recurrent prostate cancer in patients who have undergone radical prostatectomy. By contrast, diffusion-weight MRI and 1H-MRS data may improve the sensitivity of T2WI for patients who have undergone radiotherapy.

Advanced aerostatic stability analysis of suspension bridges

Xiao, Ru-Cheng,Cheng, Jin Techno-Press 2004 Wind and Structures, An International Journal (WAS Vol.7 No.1

Aerostatic instability of a suspension bridge may suddenly appears when the deformed shape of the structure produces an increase in the value of the three components of displacement-dependent wind loads distributed in the structure. This paper investigates the aerostatic stability of suspension bridges using an advanced nonlinear method based on the concept of limit point instability. Particular attention is devoted to aerostatic stability analysis of symmetrical suspension bridges. A long-span symmetrical suspension bridge (Hu Men Bridge) with a main span of 888 m is chosen for analysis. It is found that the initial configuration (symmetry or asymmetry) may affect the instability configuration of structure. A finite element software for the nonlinear aerostatic stability analysis of cable-supported bridges (NASAB) is presented and discussed. The aerostatic failure mechanism of suspension bridges is also explained by tracing aerostatic instability path.

Structure and bioactivity of triterpenoids from the stems of Schisandra sphenanthera

Cheng-Qin Liang,Rong-Hua Luo,Ju-Ming Yan,Yan Li,Xiao-Nian Li,Yi-Ming Shi,Shan-Zhai Shang,Zhong-Hua Gao,Liu-Meng Yang,Yong-Tang Zheng,Wei-Lie Xiao,Hong-Bin Zhang,Han Dong Sun 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.2

Two new triterpenoids, schisphendilactoneA and B (1 and 2), together with three known triterpenoids,were isolated from the stems of Schisandra sphenanthera. Their structures were elucidated by spectroscopic methods,and the absolute configuration of 1 was determined bysingle-crystal X-ray diffraction. Compound 2 showedmoderate inhibitory activity against SW480 cancer cellline, and compound 5 exhibited promising anti-HIV-1activity with EC50 value of 0.52 lg ml-1 and therapeuticindex value of 117.12.

Serviceability reliability analysis of cable-stayed bridges

Cheng, Jin,Xiao, Ru-Cheng Techno-Press 2005 Structural Engineering and Mechanics, An Int'l Jou Vol.20 No.6

A reliability analysis method is proposed in this paper through a combination of the advantages of the response surface method (RSM), finite element method (FEM), first order reliability method (FORM) and the importance sampling updating method. The accuracy and efficiency of the method is demonstrated through several numerical examples. Then the method is used to estimate the serviceability reliability of cable-stayed bridges. Effects of geometric nonlinearity, randomness in loading, material, and geometry are considered. The example cable-stayed bridge is the Second Nanjing Bridge with a main span length of 628 m built in China. The results show that the cable sag that is part of the geometric nonlinearities of cable-stayed bridges has a major effect on the reliability of cable-stayed bridge. Finally, the most influential random variables on the reliability of cable-stayed bridges are identified by using a sensitivity analysis.

Relationship Between PTEN and Livin Expression and Malignancy of Renal Cell Carcinomas

Cheng, Tao,Zhang, Jian-Guo,Cheng, Yuan-He,Gao, Zhong-Wei,Ren, Xiao-Qiang Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.6

Objectives: This study focused on PTEN and Livin expression and associations with malignancy in human renal clear cell carcinomas (RCCC). Methods: PTEN and Livin expression was assessed in 100 RCCC tissue samples, 50 paracarcinoma cases, and 20 normal renal tissue samples using the immunohistochemical Streptavidin proxidase (SP) method. The relationships between binding and corresponding biological characteristics, such as histological grade, lymph node metastases, and clinical stages were analyzed. Results: Positive PTEN expression in RCCC was significantly lower than in renal tissue adjacent to carcinoma tissue and normal renal tissue (P<0.01). Livin expression in the renal tissue adjacent to the carcinoma and normal renal tissues exhibited only low levels, whereas overall Livin expression in RCCC was statistically significant (P<0.01). In RCCC, PTEN expression rate gradually decreased with an increase in clinical stage, whereas that of Livin increased to statistically significant levels (P<0.01), PTEN and Livin levels being negatively correlated (r=-0.395, P<0.01). Conclusions: PTEN and Livin are important in RCCC development. The two factors combined are expected to provide indices for estimating RCCC malignancy and progression levels, as well as references for RCCC diagnosis and treatment.

Application of inverse reliability method to estimation of cable safety factors of long span suspension bridges

Cheng, Jin,Xiao, Ru-Cheng Techno-Press 2006 Structural Engineering and Mechanics, An Int'l Jou Vol.23 No.2

An efficient and accurate algorithm is proposed to estimate cable safety factor of suspension bridges satisfying prescribed reliability levels. Uncertainties in the structure and load parameters are incorporated. The proposed algorithm integrates the concepts of the inverse reliability method and deterministic method for assessing cable safety factors of suspension bridges. The unique feature of the proposed method is that it offers a tool for cable safety assessment of suspension bridges, when the reliability level is specified as a target to be satisfied by the designer. After the accuracy and efficiency of the method are demonstrated through two numerical examples, the method is used to estimate cable safety factors of suspension bridges with span length ranging from 2000 to 5000 m. The results show that the deterministic method overestimates cable safety factor of suspension bridges because of neglecting the parameter uncertainty effects. The actual cable safety factor of suspension bridges should be estimated based on the proposed method.

Construction and Differential Screening of a cDNA Library Specific to Osmotic Stress of Haloxylon ammodendron Seedlings

( Xiao Cheng Jiang ),( Xin Hong Guo ),( Xiao Ling Pan ),( Song Quan Song ) 생화학분자생물학회 2004 BMB Reports Vol.37 No.5

A subtracted cDNA library specific to osmotic stress of Haloxylon ammodendron (Mey.) Bge seedlings was constructed by suppression subtractive hybridization (SSH) and T/A cloning. SSH was performed between two groups of H. ammodendron seedlings, one was cultivated in Hoagland (H) solution as a driver and the other group was treated with osmotic stress of the Hoagland solution by the addition of 400 mM mannitol (M), as a tester. The library consisted of about 400 recombinant clones, with the average size being of 500 bp, ranging from 300 bp to 1500 bp. Using a PCR-select differential screening kit, 100 recombinant clones were randomly chosen from the subtracted cDNA library and hybridized with forward,reverse subtracted and unsubtracted probes for two rounds. As a result, 21 positive clones specific to osmotic stress were obtained and some of them were verified by Northern blot analysis. The sequencing analysis of 6 positive clones and the following homology comparison to GenBank [blastx] non-redundant databases characterized that two sequences obtained in this experiment may contribute to novel drought-related genes.