Broad-specificity amino acid racemase, a novel non-antibiotic selectable marker for transgenic plants

Yi‑Chia Kuan,Venkatesan Thiruvengadam,Jia‑Shin Lin,Jia‑Hsin Liu,Tsan‑Jan Chen,Hsin‑Mao Wu,Wen‑Ching Wang,Liang‑Jwu Chen 한국식물생명공학회 2018 Plant biotechnology reports Vol.12 No.1

The broad-specificity amino acid racemase (Bsar) from Pseudomonas putida catalyzes the racemization of various amino acids, offering a flexible and feasible platform to develop a new non-antibiotic selectable marker system for plant transformation. In the present study, we demonstrated that a Bsar variant, Bsar-R174K, that is useful as a selectable marker gene in Arabidopsis and rice that were susceptible to l-lysine and D-alanine. The introduction of wild-type Bsar, Bsar-R174K or Bsar-R174A into E. coli lysine or asparagine auxotrophs was able to rescue the growth of these microorganisms in minimal media supplemented with selectable amino acid enantiomers. The transformation of Arabidopsis with Bsar or Bsar variants based on d-alanine selection revealed that Bsar-R174K had the greatest efficiency (2.40%), superior to kanamycin selectionbased transformation (1.10%). Whereas, l-lysine-based selection exhibited lower efficiency for Bsar-R174K (0.17%). The progenies of selected Bsar-R174K transgenic Arabidopsis revealed normal growth properties. In addition, Bsar-R174K transgenic rice was obtained on l-lysine medium with an efficiency of 0.9%, and the progenies of the transgenic rice revealed morphologically normal phenotypes comparable with their wild-type counterparts. This study presents the first report of broad range amino acid racemase Bsar-R174K as a non-antibiotic selectable marker system applied in transgenic plants.

從影響層面着中國小說的歷史定位 : 以靑心才人 <<金雲翹傳>>爲例

陳益源 中國語文硏究會 1998 中國語文論叢 Vol.15 No.-

Origin

( Chen Ching-yi ) 한국기초조형학회 2018 한국기초조형학회 작품집 Vol.2018 No.2

Examining the Predictors of Turnover Behavior in Newly Employed Certified Nurse Aides: A Prospective Cohort Study

Chen Hsiao-Ching,Wang Jiun-Yi,Lee Ya-Chen,Yang Shang-Yu 한국산업안전보건공단 산업안전보건연구원 2023 Safety and health at work Vol.14 No.2

Background: The turnover rate of nurse aides in Taiwan is high. However, the predictors of turnover behavior in the newly employed are still unclear. Objective: To examine the predictors of turnover behavior in newly employed licensed nurse aides. Methods: A longitudinal study design was used and subjects were newly employed certified nurse aides from a nurse aid training association in Taiwan. A total of five questionnaire surveys were conducted. The questionnaire was mainly used to collect information on turnover behavior, personal socioeconomic background, workplace psychosocial hazards, worker health hazards, and musculoskeletal disorders. Results: A total of 300 participants were recruited in the study. Cox regression analysis results showed that short working experience (hazard ratio [HR] = 0.21, p < 0.01), work as non-home nurse aides (HR = 0.58, p = 0.01), low monthly salary (HR = 0.68, p < 0.01), high work mental load (HR = 1.01, p = 0.01), low workplace justice (HR = 0.97, p < 0.01), high workplace violence (HR = 1.60, p < 0.01), high burnout (HR = 1.01, p = 0.04), poor mental health (HR = 1.06, p = 0.04), and high total number of musculoskeletal disorder sites (HR = 1.08, p = 0.01) contribute to a higher risk of turnover. Conclusion: The results indicated that employment period, work as a home nurse aide, monthly salary, work mental load, workplace justice, workplace violence, work-related burnout, mental health, and total number of musculoskeletal disorder sites are predictors of turnover behavior in newly employed certified nurse aides.

Alternating-Projection-Based Channel Estimation for Multicell Massive MIMO Systems

Chen, Yi Liang,Ran, Rong,Oh, Hayoung The Korea Institute of Information and Commucation 2018 Journal of information and communication convergen Vol.16 No.1

In massive multiple-input multiple-output (MIMO) systems, linear channel estimation algorithms are widely applied owing to their simple structures. However, they may cause pilot contamination, which affects the subsequent data detection performance. Therefore, herein, for an uplink multicell massive multiuser MIMO system, we consider using an alternating projection (AP) for channel estimation to eliminate the effect of pilot contamination and improve the performance of data detection in terms of the bit error rates as well. Even though the AP is nonlinear, it iteratively searches the best solution in only one dimension, and the computational complexity is thus modest. We have analyzed the mean square error with respect to the signal-to-interference ratios for both the cooperative and non-cooperative multicell scenarios. From the simulation results, we observed that the channel estimation results via the AP benefit the following signal detection more than that via the least squares for both the cooperative and non-cooperative multicell scenarios.

GreenIoT Architecture for Internet of Things Applications

( Yi-wei Ma ),( Jiann-liang Chen ),( Yung-sheng Lee ),( Hsin-yi Chang ) 한국인터넷정보학회 2016 KSII Transactions on Internet and Information Syst Vol.10 No.2

A power-saving mechanism for smartphone devices is developed by analyzing the features of data that are received from Internet of Things (IoT) sensors devices to optimize the data processing policies. In the proposed GreenIoT architecture for power-saving in IoT, the power saving and feedback mechanism are implemented in the IoT middleware. When the GreenIoT application in the power-saving IoT architecture is launched, IoT devices collect the sensor data and send them to the middleware. After the scanning module in the IoT middleware has received the data, the data are analyzed by a feature evaluation module and a threshold analysis module. Based on the analytical results, the policy decision module processes the data in the device or in the cloud computing environment. The feedback mechanism then records the power consumed and, based on the history of these records, dynamically adjusts the threshold value to increase accuracy. Two smart living applications, a biomedical application and a smart building application, are proposed. Comparisons of data processed in the cloud computing environment show that the power-saving mechanism with IoT architecture reduces the power consumed by these applications by 24% and 9.2%.

Control Design of the Brushless Doubly-Fed Machines for Stand-Alone VSCF Ship Shaft Generator Systems

Yi Liu,Wu Ai,Bing Chen,Ke Chen 전력전자학회 2016 JOURNAL OF POWER ELECTRONICS Vol.16 No.1

This paper presents a stand-alone variable speed constant frequency (VSCF) ship shaft generator system based on a brushless doubly-fed machine (BDFM). In this system, the output voltage amplitude and frequency of the BDFM are kept constant under a variable rotor speed and load by utilizing a well-designed current vector controller to regulate the control winding (CW) current. The control scheme is proposed, and the hardware design for the control system is developed. The proposed generator system is tested on a 325 TEU container vessel, and the test results show the good dynamic performance of the CW current vector controller and the whole control system. A harmonic analysis of the output voltage and a fuel consumption analysis of the generator system are also implemented. Finally, the total efficiency of the generator system is presented under different rotor speeds and load conditions.

Identification of DNA methylation and genetic alteration simultaneously from a single blood biopsy

Chen Xiaomin,Liu Jiahui,Li Jun,Xie Yinpeng,Yu Zichen,Shen Lu,Liu Qingfeng,Wu Wei,Zhao Qiang,Lin Haoxiang,Liu Gaotong,Luo Qiuping,Yang Ling,Huang Yi,Zhao Meiru,Yi Xin,Xia Xuefeng 한국유전학회 2023 Genes & Genomics Vol.45 No.5

Background High-throughput sequencing of blood cell-free DNA (cfDNA) techniques offer an opportunity to characterize and monitor cancer rapidly in a non-invasive and real-time manner. Nonetheless, there lacks a tool within therapeutic arsenal to identify multi-omics alterations simultaneously from a single biopsy. In current times, bisulfite-based sequencing detects 5mC and 5hmC at single-base resolution is the golden standard of DNA methylation, while the degradation of DNA and biased sequencing data are the problems of this method. Objective To identify the consistency analysis of methylation and genetic variation with single library, we presented a platform detecting multi-omics data simultaneously from a single blood biopsy using bisulfite-free method of genomic methylation sequencing (GM-seq) mediated by TET enzyme. Methods We detected methylomic and genetic changes simultaneously from a single blood biopsy in NA12878 and randomly chose ten blood biopsies from colorectal cancer or lung cancer patients to validate the ability of GM-seq. Results Similar cytosine methylation level between whole genome bisulfite sequencing (WGBS) and GM-seq were identified in NA12878. Moreover, longer insert size, CpGs coverage and GC distribution were outperformed than WGBS. In addition, the comparison of the single nucleotide polymorphism (SNP), insertion-deletion (Indel) and copy number variation (CNV) in NA12878 or ctDNA from liver cancer between GM-seq and whole genome sequencing (WGS) show a good consistency, indicating that this method is feasible for detecting genetic variation in blood. Conclusion In conclusion, our work demonstrated a method for identification of the methylated modification and genetic variations simultaneously from a single blood biopsy.

Two-dimensional Elastic Analysis of Doubly Periodic Circular Holes in Infinite Plane

Yi-Zhou Chen,Kang Yong Lee 대한기계학회 2002 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.16 No.5

Two-dimensional elastic analysis of doubly periodic circular holes in an infinite plane is given in this paper. Two cases of loading, remote tension and remote shear, are considered. A rectangular cell is cut from the infinite plane. In both cases, the boundary value problem can be reduced to a complex mixed one. It is found that the eigenfunction expansion variational method is efficient to solve the problem. Based on the deformation response under certain loading, the notched medium could be modeled by an orthotropic medium without holes. Elastic properties for the equivalent orthotropic medium are investigated, and the stress concentration along the hole contour is studied. Finally, numerical examples and results are given.

Factors Related to the Home Teaching of English Language to Preschool aged Children: A Taiwanese Study

Yi-Chen Lan,Sheila Degotardi,Jane Torr 환태평양유아교육연구학회 2011 Asia-Pacific journal of research in early childhoo Vol.5 No.2

Issues surrounding the introduction of English as a foreign language (EFL) instruction to children during the early childhood period are subject to much debate. Official government policies may not always reflect actual parental practices. Little is known about the extent to which explicit EFL teaching by mothers occurs in the home context. This study investigated whether Taiwanese mothers of 3 to 6 year old children were teaching EFL to their children at home and the characteristics associated with this practice. A sample of 466 mothers of preschoolers completed a questionnaire identifying their home teaching practices and assessing their attitudes towards teaching EFL. Results indicated a positive trend towards home EFL teaching, with teaching mothers tending to be aged between 30-39 years, more highly educated and more confident about their own English abilities than those who were not teaching. The findings raise significant issues that can inform EFL teaching policy and practice during the early childhood years.