Verification of Wisteria floribunda agglutinin-positive glycoproteins as a cholangiocarcinoma marker

Atsushi Matsuda,Atsushi Kuno,Hideki Matsuzaki,Toru Kawamoto,Toshihide Shikanai,Yasuni Nakanuma,Masakazu Yamamoto,Nobuhiro Ohkohchi,Yuzuru Ikehara,Junichi Shoda,Jun Hirabayashi,Hisashi Narimatsu 한국당과학회 2012 한국당과학회 학술대회 Vol.2012 No.1

Cholangiocarcinoma (CC) is a lethal malignancy which exhibits asymptomatic growth infiltrating the surrounding structures, and thus,CC is usually detected at an advanced stage. The mainstay of treatment for CC is complete resection with negative surgical margins. Therefore, its diagnosis at a relatively early stage is demanded for performing the surgical resection. Since the definitive CC diagnosis relies on invasive methods such as biliary cytology and biopsy, a noninvasive assay with high diagnostic accuracy is keenly required. In the previous meeting, we reported that Wisteria floribunda agglutinin (WFA) is the best probe lectin which reliably distinguishes between CC and normal bile duct epithelia in tissue sections. Moreover, L1 cell adhesion molecule (L1CAM), CA125, and maspin were assigned as the reliable CC marker candidates by WFA-assisted glycoproteomics and immunohistochemistry. In this meeting, we will introducethe verification and validation process in one of the above candidates, L1CAM. Since the serum concentration of L1CAM was low as described in other reports (< 5 ng/mL), we firstly constructed a highly-sensitive detection system to confirm the existence of L1CAM in both bile and serum of CC patients with immunoprecipitation and western blotting. We then performed highly-sensitive glycan profiling with antibody-assisted lectin microarray (limit of detection: 25 pg) and confirmed WFA-positivity of biliary L1CAM from the CC patients. The subsequent validation study using bile samples from CC patients (n = 29) and patients with benign bile duct diseases (n = 29) showed that WFA-positive L1CAM distinguished CC from the benigndiseases with good specificity (sensitivity = 0.66, specificity = 0.93, overall accuracy = 0.79, area under the receiver operating curve [AUC] = 0.82). The combined use of the L1CAM assay with the highly-sensitive assay detecting WFA-positive sialylated mucin 1 (WFA-sialyl MUC1), a reliable CC marker (Matsuda A., et al., Hepatology, 2010), sufficiently improved the diagnostic accuracy of CC (overall accuracy = 0.84, AUC = 0.93). This combination assay using WFA–L1CAM and WFA–sialyl MUC1 will possibly be a useful serological test with enhanced reliability.

Elucidation of the Noise in Corrosion of Aluminum Foil

( Atsushi Chiba ),( Atsushi Hattori ),( Weng Chang Wu ) 한국부식방식학회(구 한국부식학회) 2004 Corrosion Science and Technology Vol.3 No.3

Investigation and design of a new shock absorbing device that cooperates between two colliding objects

Atsushi Kawaguchi,Kai Sedlaczek,Atsushi Kawamoto,Peter Eberhard 대한기계학회 2009 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.23 No.4

This paper deals with a new type of shock absorbing device that cooperates between two colliding objects. The new device utilizes a four-bar-chain-like articulated mechanism with some possible actuations. The devices are assumed to be deployed in the pre-crash phase (by sensing and identifying unavoidable collisions) so as to provide an extended deformable region between the two objects. Moreover, by functioning like a four-bar-chain mechanism, they produce a repulsive effect by pushing each other and sliding in the opposite lateral direction. To investigate the capacity of the proposed articulated shock absorbing mechanism, a standard numerical optimization technique called SQP and a new optimization technique called ALPSO are applied. ALPSO is an attractive method for solving multimodal optimization problems based on Particle Swarm Optimization and constraint treatment using an Augmented Lagrange Method. We demonstrate ALPSO and show its applicability to this problem. The optimization process automatically determines the mode of the operation and gives an estimation of the development potential of the new device.

Minimally invasive radical hysterectomy and the importance of avoiding cancer cell spillage for early-stage cervical cancer: a narrative review

Atsushi Fusegi,Hiroyuki Kanao,Shiho Tsumura,Atsushi Murakami,Akiko Abe,Yoichi Aoki,Hidetaka Nomura 대한부인종양학회 2023 Journal of Gynecologic Oncology Vol.34 No.1

Radical hysterectomy is a standard surgery to treat early-stage uterine cervical cancer. The Laparoscopic Approach to Cervical Cancer (LACC) trial has shown that patients receiving minimally invasive radical hysterectomy have a poorer prognosis than those receiving open radical hysterectomy; however, the reason for this remains unclear. The LACC trial had 2 concerns: the learning curve and the procedural effects. Appropriate management of the learning curve effect, including surgeons’ skills, is required to correctly interpret the result of surgical randomized controlled trials. Whether the LACC trial managed the learning curve effect remains controversial, based on the surgeons’ inclusion criteria and the distribution of institutions with recurrent cases. An appropriate surgical procedure is also needed, and avoiding intraoperative cancer cell spillage plays an important role during cancer surgery. Cancer cell spillage during minimally invasive surgery to treat cervical cancer is caused by several factors, including 1) exposure of tumor, 2) the use of a uterine manipulator, and 3) direct handling of the uterine cervix. Unfortunately, these issues were not addressed by the LACC trial. We evaluated the results of minimally invasive radical hysterectomy while avoiding cancer cell spillage for early-stage cervical cancer. Our findings show that avoiding cancer cell spillage during minimally invasive radical hysterectomy may ensure an equivalent oncologic outcome, comparable to that of open radical hysterectomy. Therefore, evaluating the importance of avoiding cancer cell spillage during minimally invasive surgery with a better control of the learning curve and procedural effects is needed.

Glycomics meets histopathology: Lectin microarray can profile the whole glycoproteins in specific groups of cells isolated by laser microdissection

Atsushi Kuno,Masaharu Nomura,Hideki Matsuzaki,Tomoko Nakagawa,Atsushi Matsuda,Yoshitoshi Hirao,Masao Sasaki,Norihiro Ikeda,Toshitaka Nagao,Yuzuru Ikehara,Hisashi Narimatsu 한국당과학회 2012 한국당과학회 학술대회 Vol.2012 No.1

Cell glycome is defined by the glyco synthesis machinery regulated by harmonized expression of more than 100 glycogenes. The machinery-dependent glycome drastically shifts during cell progression and differentiation in association with tumorigenesis and malformation, and thus it motivates us to discover the disease-related alteration in glycosylation. Glycan-targeted histochemical approaches using lectin and anti-glycogene antibodies have provided some key information to characterize specific histological types of cells in pathology. However, this approach is not suitable for the comprehensive analysis targeting the cell glycome, and thus may fail to provide insight into glycome shift during the disease progression. Several years ago, we developed the methodology for rapid and systematic glycome shift analysis targeting formalin-fixed tissue specimens by means of lectin microarray. The resultant method enabled simultaneous observation of over 40 lectins interacted with glycoproteins in 1 mm2 of the tissue specimens. Recently, we sophisticated this methodology to be suitable for comparative analysis of a series of cells in specific groups isolated from a single tissue specimen by laser microdissection, and now our research has gained interest in the variability and distribution of cell glycome in the tissue, i.e., “tissue glycome mapping”. In this meeting, we will summarize the advantage of this new methodology and its application for glyco-biomarker discovery, as well as the construction of “tissue glycome atlas”.

Reduction of acetaldehyde formation from pulverized solid woods by thermal and chemical treatments

Mizukoshi, Atsushi,Kurosaki, Yosuke,Yamamoto, Naomichi,Noguchi, Miyuki,Iizuka, Atsushi,Yamasaki, Akihiro,Yanagisawa, Yukio Springer-Verlag 2018 Wood science and technology Vol.52 No.2

<P>High concentrations of acetaldehyde are often observed in indoor environments. One possible source of this is acetaldehyde formation produced by ethanol from wood building materials. To characterize indoor acetaldehyde formation and propose methods for its reduction, chamber-based experiments were conducted in which alcohols were added to pulverized solid woods. The results indicated that acetaldehyde was generated from the ethanol-added wood materials, and softwoods, namely Japanese cedar, Japanese cypress, and Swedish redwood generated more acetaldehyde than the other three woods. The experiments using different types of alcohols of methanol, ethanol, 1-propanol, and 2-propanol revealed a tendency similar to the reported enzymatic profile of the higher plant-derived alcohol dehydrogenase. It was confirmed that acetaldehyde generation was reduced by treatment methods typically used for inactivating enzymes, namely chemical treatments using pyrazole, borate, and urea and thermal treatments. The findings suggest that the chemical and thermal treatment methods are useful to reduce acetaldehyde generation in indoor environments.</P>

A Brief History of Monetary Policy in Post-war Japan : Credit Squeezing and Foreign Exchange Policy

Ichinose, Atsushi 啓明大學校 産業經營硏究所 2002 經營經濟 Vol.35 No.1

일본 모모야마대학 Atsushi Inchinose 교수님이 발표한 A Brief History of Monetary policy in Post-wat Japan의 논문을 요약하며 다음과 같다. 첫째, 일본정부는 외환정책(외환통제정책과 환율정책)을 활용하여 경제성장을 달성 하려고 추구해왔다. 이러한 강제적인 외환정책으로 인하여 일본의 통화긴축정책은 그 시점이 5차례나 늦어지게 되었다. 이와 같이 지연된 통화정책 때문에 외부비경제, 저축가지의 감소 및 장기적인 불황이 초래되었다. 둘째, 고속성장기의 1970년대까지 일본의 통화정책은 국제수지연건에 의해 제약을 받아왔다. 예를 들어, 국제수지가 적자인 경우 일본은행은 공정할인율수단이나 신용재분수단을 통해 기축금융정책을 취해야 한다. 그러나 봅 논문의 주장에 의하면 국제 수지와 통화정책간의 관계만을 관찰하는 것은 불충분하다는 것이다. 즉, 전후 일본의 통화정책에서는 재무성의 외환통제와 외환보유고 정책이 고려되어야 한다는 것이다. 본 논문은 현재의 경제적 불황을 통화정책의 실패에서 찾으려는 흥미 있는 논문으로 평가된다. 즉 외환정책을 너무 강조한 나머지 긴축종화정책의 시기가 너무 늦게 이루어졌다는 것이다. 그러나 논평자는 다음과 같은 두 가지 보완점을 지적하고 싶다. 첫째 기본적으로 통화정책의 목표는 경제성장과 물가안정을 달성하는 것이다. 실제로 한 나라의 통화정책에 영향을 주는 요인들은 많다. 본 논문에서도 통화긴축시점별로 통화정책에 영향을 준 요인들을 보여주었다. 특히 1970년대 이후 많은 나라들이 금리위주의 통화정책을 시행해왔다. 예를 들어 단기금리의 조정을 통해 금융시장, 자산시장, 외환시장 및 시장참가자의 기대에 영향을 주면, 이를 통해 소비, 투자 그리고 수출입에 영향을 주어 최종적으로는 경제성장과 물가안정 등 통화정책의 목적을 달성할 수가 있는 것이다. 따라서 특정의 통화정책에 대한 원인을 규명하는 것은 매우 어렵다고 본다. 본 논문은 정부의 외환정책과 통화정책지연간의 관계를 찾으려고 시도하였다. 그러나 그 논리가 다소 불분명하다. 즉, 본 논문은 사후적 관계를 도축했을 뿐, 이론적(사전적)관계를 도출하는 데에는 한계가 있다. 둘째, 마찬가지로 실증분석을 위해서는 통계적인 유의수준을 활용하여 검정하는 것이 필요하다고 본다.

Current understanding of primary biliary cholangitis

( Atsushi Tanaka ) 대한간학회 2021 Clinical and Molecular Hepatology(대한간학회지) Vol.27 No.1

Primary biliary cholangitis (PBC) causes chronic and persistent cholestasis in the liver, eventually resulting in cirrhosis and hepatic failure without appropriate treatment. PBC mainly develops in middle-aged women, but it is also common in young women and men. PBC is considered a model of autoimmune disease because of the presence of disease-specific autoantibodies, that is, antimitochondrial antibodies (AMAs), intense infiltration of mononuclear cells into the bile ducts, and a high prevalence of autoimmune diseases such as comorbidities. Histologically, PBC is characterized by degeneration and necrosis of intrahepatic biliary epithelial cells surrounded by a dense infiltration of mononuclear cells, coined as chronic non-suppurative destructive cholangitis, which leads to destructive changes and the disappearance of small- or medium-sized bile ducts. Since 1990, early diagnosis with the detection of AMAs and introduction of ursodeoxycholic acid as first-line treatment has greatly altered the clinical course of PBC, and liver transplantation-free survival of patients with PBC is now comparable to that of the general population. (Clin Mol Hepatol 2021;27:1-21)

NiO Thin Films with Self-patterned Surfaces for Templates of Nanoparticles

Atsushi Sugihara,Seiji Mitani,Koki Takanashi 한국자기학회 2007 한국자기학회 학술연구발표회 논문개요집 Vol.- No.-

Effect of alendronate on muscle mass: Investigation in patients with osteoporosis

Atsushi Harada,Sadayuki Ito,Yasumoto Matsui,Yoshihito Sakai,Marie Takemura,Haruhiko Tokuda,Tetsuro Hida,Hiroshi Shimokata 대한골다공증학회 2015 Osteoporosis and Sarcopenia Vol.1 No.1

Objectives: Many osteoporosis drugs reliably increase bone mass in the elderly; if these drugs also had a positive effect on muscle, their benefit would be even greater. We examined the effect of alendronate monotherapy on muscle mass in patients with osteoporosis. Methods: In this retrospective cohort, case-control study, patients from an osteoporosis database were divided into 2 groups: alendronate-treated patients (group A; n ¼ 199) and a control group receiving no drug treatment (group C; n ¼ 233). Appendicular skeletal muscle mass (ASM) and skeletal muscle mass index (SMI) measured by dual-energy X-ray absorptiometry were assessed at approximately 1 year. The change in muscle mass was compared between the groups. Results: At baseline, group A included more women and had lower height, weight, bone mineral content, and muscle mass than group C. A comparison of changes after 1 yeardadjusted for age, sex, observation period, body mass index and initial valuesdrevealed that the muscle mass in group A showed increases by 0.137 kg/m2 in SMI, 514 g in ASM, and 319 g in lower limb muscle mass (LLM). Group C showed no changes in muscle mass. A significant difference in the amount of change in ASM and LLM was found between the groups after adjustment: 2.5 times and 4.4 times higher, respectively, in groups A and C. However, the difference in SMI disappeared after adjustment. Conclusions: This is the first study to show that alendronate may have a positive effect not only on bone, but on muscle as well.